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WO2008112980A3 - Method and system for assembly of macromolecules and nanostructures - Google Patents

Method and system for assembly of macromolecules and nanostructures Download PDF

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Publication number
WO2008112980A3
WO2008112980A3 PCT/US2008/057013 US2008057013W WO2008112980A3 WO 2008112980 A3 WO2008112980 A3 WO 2008112980A3 US 2008057013 W US2008057013 W US 2008057013W WO 2008112980 A3 WO2008112980 A3 WO 2008112980A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanostructures
macromolecules
assembly
template
peptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/057013
Other languages
French (fr)
Other versions
WO2008112980A2 (en
Inventor
Vincent Suzara
Paul Bentley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Incitor Inc
Original Assignee
Incitor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/936,045 external-priority patent/US20090118140A1/en
Application filed by Incitor Inc filed Critical Incitor Inc
Publication of WO2008112980A2 publication Critical patent/WO2008112980A2/en
Publication of WO2008112980A3 publication Critical patent/WO2008112980A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Nanotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A template-based system enables the assembly of macromolecules and nanostructures. The template system comprises a plurality of single strand DNA molecules which are substantially parallel, substantially inline each from one end, and substantially equally spaced apart, wherein each DNA molecule has a distinguishable length and a known sequence. The system can be used for the precise, accurate, and efficient synthesis of peptides, proteins and enzymes.
PCT/US2008/057013 2007-03-15 2008-03-14 Method and system for assembly of macromolecules and nanostructures Ceased WO2008112980A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US91814407P 2007-03-15 2007-03-15
US60/918,144 2007-03-15
US11/936,045 2007-11-06
US11/936,045 US20090118140A1 (en) 2007-03-15 2007-11-06 Method and system for assembly of macromolecules and nanostructures

Publications (2)

Publication Number Publication Date
WO2008112980A2 WO2008112980A2 (en) 2008-09-18
WO2008112980A3 true WO2008112980A3 (en) 2008-11-06

Family

ID=39760417

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/057013 Ceased WO2008112980A2 (en) 2007-03-15 2008-03-14 Method and system for assembly of macromolecules and nanostructures

Country Status (1)

Country Link
WO (1) WO2008112980A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8993714B2 (en) 2007-10-26 2015-03-31 Imiplex Llc Streptavidin macromolecular adaptor and complexes thereof
BRPI0911937A2 (en) * 2008-08-06 2015-08-04 Incitor Inc Production method of a product and an item produced
US9102526B2 (en) 2008-08-12 2015-08-11 Imiplex Llc Node polypeptides for nanostructure assembly
WO2011153211A1 (en) 2010-06-01 2011-12-08 President And Fellows Of Harvard College Apparatus for measurement of spinning forces relating to molecules
US9869851B2 (en) 2008-12-02 2018-01-16 The Research Foundation For The State University Of New York Centrifuge force microscope modules and systems for use in a bucket of a centrifuge
US8491454B2 (en) 2008-12-02 2013-07-23 President And Fellows Of Harvard College Spinning force apparatus
WO2010132363A1 (en) * 2009-05-11 2010-11-18 Imiplex Llc Method of protein nanostructure fabrication
WO2017147398A1 (en) 2016-02-25 2017-08-31 Children's Medical Center Corporation Spinning apparatus for measurement of characteristics relating to molecules
WO2017222711A1 (en) * 2016-06-22 2017-12-28 The Charles Stark Draper Laboratory, Inc. Composition for use in molecular chain synthesis
CN111198175B (en) * 2018-11-19 2022-09-27 中国科学院宁波材料技术与工程研究所 Two-dimensional array of macroscopic large-area gold nanorods with controllable distribution of hot spots and its application
WO2025196169A2 (en) * 2024-03-20 2025-09-25 Nuclera Ltd High throughput protein expression screening

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000061775A1 (en) * 1999-04-08 2000-10-19 Pavel Sergeev Synthesis of biologically active compounds in cells
US20050158763A1 (en) * 2003-12-19 2005-07-21 Albena Ivanisevic Aligned long DNA molecules on templates and methods for preparing

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000061775A1 (en) * 1999-04-08 2000-10-19 Pavel Sergeev Synthesis of biologically active compounds in cells
US20050158763A1 (en) * 2003-12-19 2005-07-21 Albena Ivanisevic Aligned long DNA molecules on templates and methods for preparing

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BROCKMAN J.M. ET AL.: "A multistep chemical modification procedure to create DNA arrays on gold surfaces for the study of protein-DNA interactions with surface plasmon resonance imaging", J. AM. CHEM. SOC., vol. 121, no. 35, 1999, pages 8044 - 8051, XP002949098 *
WALDER J.A. ET AL.: "Complementary carrier peptide synthesis: general strategy and implications for prebiotic origin of peptide synthesis", PROC. NATL. ACAD. SCI. USA, vol. 76, no. 1, January 1979 (1979-01-01), pages 51 - 55, XP000857351 *

Also Published As

Publication number Publication date
WO2008112980A2 (en) 2008-09-18

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