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WO2008112683A3 - Synthèse de gènes par amplification d'assemblages circulaires - Google Patents

Synthèse de gènes par amplification d'assemblages circulaires Download PDF

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Publication number
WO2008112683A3
WO2008112683A3 PCT/US2008/056504 US2008056504W WO2008112683A3 WO 2008112683 A3 WO2008112683 A3 WO 2008112683A3 US 2008056504 W US2008056504 W US 2008056504W WO 2008112683 A3 WO2008112683 A3 WO 2008112683A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene synthesis
circular assembly
assembly amplification
amplification
polynucleotide sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/056504
Other languages
English (en)
Other versions
WO2008112683A2 (fr
Inventor
George M Church
Duhee Bang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of WO2008112683A2 publication Critical patent/WO2008112683A2/fr
Publication of WO2008112683A3 publication Critical patent/WO2008112683A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention porte sur des compositions et des méthodes de synthèse de polynucléotides, de réduction des erreurs lors de la synthèse des polynucléotides, de correction des disparités dans les séquences de polynucléotides de synthèse, et de sélection de séquences de polynucléotides correctement assemblées.
PCT/US2008/056504 2007-03-13 2008-03-11 Synthèse de gènes par amplification d'assemblages circulaires Ceased WO2008112683A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US89457307P 2007-03-13 2007-03-13
US60/894,573 2007-03-13
US98951707P 2007-11-21 2007-11-21
US60/989,517 2007-11-21

Publications (2)

Publication Number Publication Date
WO2008112683A2 WO2008112683A2 (fr) 2008-09-18
WO2008112683A3 true WO2008112683A3 (fr) 2008-12-18

Family

ID=39760349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/056504 Ceased WO2008112683A2 (fr) 2007-03-13 2008-03-11 Synthèse de gènes par amplification d'assemblages circulaires

Country Status (1)

Country Link
WO (1) WO2008112683A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9670517B1 (en) 2012-01-16 2017-06-06 Integrated Dna Technologies, Inc. Synthesis of long nucleic acid sequences
JP2015509005A (ja) 2012-02-01 2015-03-26 シンセティック ジェノミクス インコーポレーテッド エラーを最少限に抑える核酸分子の合成のための材料及び方法
US20210147830A1 (en) 2018-06-29 2021-05-20 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
EP4114972A1 (fr) 2020-03-06 2023-01-11 Life Technologies Corporation Synthèse et assemblage d'acide nucléique à fidélité de séquence élevée
WO2024132094A1 (fr) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Extraction de molécules d'acide nucléique à séquence vérifiée

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050069939A1 (en) * 2003-09-26 2005-03-31 Youxiang Wang Amplification of polynucleotides by rolling circle amplification
US20060223098A1 (en) * 2005-03-31 2006-10-05 Lane David J Circularizable nucleic acid probes and amplification methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050069939A1 (en) * 2003-09-26 2005-03-31 Youxiang Wang Amplification of polynucleotides by rolling circle amplification
US20060223098A1 (en) * 2005-03-31 2006-10-05 Lane David J Circularizable nucleic acid probes and amplification methods

Also Published As

Publication number Publication date
WO2008112683A2 (fr) 2008-09-18

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