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WO2008108345A1 - Insect-resistant protein and insect-resistance gene encoding the insect-resistant protein - Google Patents

Insect-resistant protein and insect-resistance gene encoding the insect-resistant protein Download PDF

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Publication number
WO2008108345A1
WO2008108345A1 PCT/JP2008/053794 JP2008053794W WO2008108345A1 WO 2008108345 A1 WO2008108345 A1 WO 2008108345A1 JP 2008053794 W JP2008053794 W JP 2008053794W WO 2008108345 A1 WO2008108345 A1 WO 2008108345A1
Authority
WO
WIPO (PCT)
Prior art keywords
insect
amino acid
acid sequence
resistant protein
resistance gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/053794
Other languages
French (fr)
Japanese (ja)
Inventor
Kotaro Konno
Naoya Wasano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NATIONAL INSTITUTE OF AGLOBIOLOGICAL SCIENCES
Original Assignee
NATIONAL INSTITUTE OF AGLOBIOLOGICAL SCIENCES
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NATIONAL INSTITUTE OF AGLOBIOLOGICAL SCIENCES filed Critical NATIONAL INSTITUTE OF AGLOBIOLOGICAL SCIENCES
Priority to BRPI0808895-0A2A priority Critical patent/BRPI0808895A2/en
Priority to CA2679926A priority patent/CA2679926C/en
Priority to US12/449,917 priority patent/US8624085B2/en
Publication of WO2008108345A1 publication Critical patent/WO2008108345A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Pest Control & Pesticides (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

[PROBLEMS] To provide an insect-resistant protein showing a sufficient resistance to insects even in a small amount; an insect-resistance gene encoding the above insect-resistant protein; a recombinant vector containing the above insect-resistance gene; a host cell and a plant cell having the recombinant vector transferred thereinto; a transformant having been transformed by the insect-resistance gene and a method for producing the same; a recovered protein having been recovered thereby; and an insect-resistant agent comprising the same as the active ingredient. [MEANS FOR SOLVING PROBLEMS] An insect-resistant protein originating in a plant which is an insect-resistant protein comprising a first amino acid sequence having a 50% or more homology with the amino acid sequence represented by SEQ ID NO:1 in Sequence Listing, a second amino acid sequence having a 50% or more homology with the amino acid sequence represented by SEQ ID NO:2 in Sequence Listing and a third amino acid sequence having a 50% or more homology with the amino acid sequence represented by SEQ ID NO:3 in Sequence Listing, and in which the second amino acid sequence has at least one spppp sequence.
PCT/JP2008/053794 2007-03-02 2008-03-03 Insect-resistant protein and insect-resistance gene encoding the insect-resistant protein Ceased WO2008108345A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
BRPI0808895-0A2A BRPI0808895A2 (en) 2007-03-02 2008-03-03 INSECT-RESISTANCE PROTEIN FROM A PLANT, INSECT RESISTANCE GENE, RECOMBINANT VECTOR, HOST CELL, C-PLANT CELL, TRANSFORMER, METHOD TO PRODUCE A TRANSFORMER, PROTEIN AND RESISTANT AGENT
CA2679926A CA2679926C (en) 2007-03-02 2008-03-03 Insect-resistant protein and insect-resistance gene encoding the insect-resistant protein
US12/449,917 US8624085B2 (en) 2007-03-02 2008-03-03 Insect resistant protein and insect-resistance gene encoding the insect-resistant protein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-052798 2007-03-02
JP2007052798 2007-03-02

Publications (1)

Publication Number Publication Date
WO2008108345A1 true WO2008108345A1 (en) 2008-09-12

Family

ID=39738223

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/053794 Ceased WO2008108345A1 (en) 2007-03-02 2008-03-03 Insect-resistant protein and insect-resistance gene encoding the insect-resistant protein

Country Status (5)

Country Link
US (1) US8624085B2 (en)
JP (1) JP4865749B2 (en)
BR (1) BRPI0808895A2 (en)
CA (1) CA2679926C (en)
WO (1) WO2008108345A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012063338A1 (en) * 2010-11-10 2012-05-18 国立大学法人京都工芸繊維大学 Protein having chitinase activity and uses thereof
CN104450739A (en) * 2014-11-22 2015-03-25 浙江大学 Rice source insect-resistant related gene OsHR1 as well as encoded product and application thereof
JPWO2021039659A1 (en) * 2019-08-23 2021-03-04

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5835727B2 (en) * 2011-08-12 2015-12-24 国立研究開発法人農業生物資源研究所 Insect resistance protein and insect resistance gene encoding the insect resistance protein

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6639050B1 (en) * 1997-07-21 2003-10-28 Ohio University Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE NCBI [O] 28 February 2008 (2008-02-28), WASANO N AND KONNO K: "Difinition: Morus alba latex protein mRNA, complete cds", XP055353635, Database accession no. EF535852 *
NAOYA WASANO ET AL.: "Kuwa Nyueki ga Yusuru Kobunshi Taichusei Inshi no Shoseijo", DAI 51 KAI JAPANESE SOCIETY OF APPLIED ENTOMOLOGY AND ZOOLOGY TAIKAI KOEN YOSHI, 1 March 2007 (2007-03-01), pages 156 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012063338A1 (en) * 2010-11-10 2012-05-18 国立大学法人京都工芸繊維大学 Protein having chitinase activity and uses thereof
CN104450739A (en) * 2014-11-22 2015-03-25 浙江大学 Rice source insect-resistant related gene OsHR1 as well as encoded product and application thereof
JPWO2021039659A1 (en) * 2019-08-23 2021-03-04

Also Published As

Publication number Publication date
US20100146668A1 (en) 2010-06-10
JP4865749B2 (en) 2012-02-01
CA2679926A1 (en) 2008-09-12
BRPI0808895A2 (en) 2014-09-02
CA2679926C (en) 2016-05-03
US8624085B2 (en) 2014-01-07
JP2008245640A (en) 2008-10-16

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