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WO2008108074A1 - 蛍光プローブ - Google Patents

蛍光プローブ Download PDF

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Publication number
WO2008108074A1
WO2008108074A1 PCT/JP2008/000392 JP2008000392W WO2008108074A1 WO 2008108074 A1 WO2008108074 A1 WO 2008108074A1 JP 2008000392 W JP2008000392 W JP 2008000392W WO 2008108074 A1 WO2008108074 A1 WO 2008108074A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluorophore
substance
measured
wavelength
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/000392
Other languages
English (en)
French (fr)
Inventor
Tetsuo Nagano
Hirotatsu Kojima
Sakiko Aizawa
Kazuki Kiyose
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Tokyo NUC
Original Assignee
University of Tokyo NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Tokyo NUC filed Critical University of Tokyo NUC
Priority to US12/527,898 priority Critical patent/US8465985B2/en
Priority to JP2009502453A priority patent/JP5559529B2/ja
Publication of WO2008108074A1 publication Critical patent/WO2008108074A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1003—Carbocyclic compounds
    • C09K2211/1007—Non-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1018—Heterocyclic compounds
    • C09K2211/1025—Heterocyclic compounds characterised by ligands
    • C09K2211/1029—Heterocyclic compounds characterised by ligands containing one nitrogen atom as the heteroatom
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/141111—Diverse hetero atoms in same or different rings [e.g., alkaloids, opiates, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Materials Engineering (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

　励起光波長／蛍光波長が生体組織透過性に優れた近赤外領域にあって測定対象物質をレシオ法で測定可能な蛍光プローブであって、蛍光団A－S－蛍光団B（蛍光団A及び蛍光団Bは600 nm～950 nmの波長の励起光を照射した場合に蛍光を発する蛍光団であって、蛍光団Aは測定対象物質との特異的な反応の前後で蛍光特性が変化する性質を有し、Sは蛍光団Aと蛍光団Bとを連結するスペーサーを示す）で表され、測定対象物質との特異的な反応の前後で蛍光団Aと蛍光団Bとの間の蛍光共鳴エネルギー効率の実質的な変化を生じる化合物を含む蛍光プローブであって、蛍光団Aが下記の式（AI）で表される蛍光プローブ。
PCT/JP2008/000392 2007-03-01 2008-02-29 蛍光プローブ Ceased WO2008108074A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/527,898 US8465985B2 (en) 2007-03-01 2008-02-29 Fluorescent probe
JP2009502453A JP5559529B2 (ja) 2007-03-01 2008-02-29 蛍光プローブ

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007050941 2007-03-01
JP2007-050941 2007-03-01

Publications (1)

Publication Number Publication Date
WO2008108074A1 true WO2008108074A1 (ja) 2008-09-12

Family

ID=39737972

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/000392 Ceased WO2008108074A1 (ja) 2007-03-01 2008-02-29 蛍光プローブ

Country Status (3)

Country Link
US (1) US8465985B2 (ja)
JP (1) JP5559529B2 (ja)
WO (1) WO2008108074A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009107769A1 (ja) * 2008-02-29 2009-09-03 国立大学法人東京大学 活性酸素測定用試薬
WO2011105610A1 (ja) * 2010-02-26 2011-09-01 国立大学法人名古屋大学 インスレーター及びその利用
US20110313129A1 (en) * 2009-02-11 2011-12-22 Life Technologies Corporation Large stokes shift dyes

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2796711A1 (en) * 2010-04-22 2011-10-27 Board Of Regents, The University Of Texas System Fluorescent nitric oxide probes and associated methods
WO2013184641A1 (en) * 2012-06-06 2013-12-12 Board Of Regents, The University Of Texas System Fluorescent nitric oxide probes and associated methods
US10067112B2 (en) 2015-09-30 2018-09-04 Battelle Memorial Institute Autonomous sensor fish to support advanced hydropower development
CN108659142B (zh) 2018-05-29 2020-09-29 苏州百源基因技术有限公司 一种荧光标记的多糖及其制备方法和用途
CN117430804B (zh) * 2023-11-28 2024-04-05 渤海大学 纯水环境快速检测次氯酸根荧光探针的制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003501540A (ja) * 1999-06-09 2003-01-14 カーネギー メロン ユニヴァーシティ 反応性蛍光試薬としてのｐＨ官能性シアニン染料
JP2004315501A (ja) * 2003-03-28 2004-11-11 Tetsuo Nagano 亜鉛蛍光プローブ

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4402191B2 (ja) 1999-02-18 2010-01-20 哲雄 長野 亜鉛蛍光プローブ
JP3692488B2 (ja) 1999-05-14 2005-09-07 独立行政法人科学技術振興機構 基質ペプチドの両端に蛍光発光化合物で修飾したカスパーゼ活性を検出する新規な蛍光プローブ
EP1394219A1 (en) 1999-06-09 2004-03-03 Carnegie-Mellon University PH sensitive cyanine dyes as reactive fluorescent reagents
CA2401359A1 (en) * 2000-02-28 2001-08-30 Daiichi Pure Chemicals Co., Ltd. Fluorescent probe for zinc
JP4309253B2 (ja) 2001-06-14 2009-08-05 哲雄 長野 亜鉛蛍光プローブ
US7696245B2 (en) * 2003-03-28 2010-04-13 Sekisui Medical Co., Ltd. Fluorescent probe for zinc
JP4759506B2 (ja) * 2004-02-23 2011-08-31 哲雄 長野 蛍光プローブ
US8178669B2 (en) * 2005-11-14 2012-05-15 The University Of Tokyo Fluorescent probe for peroxynitrite

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003501540A (ja) * 1999-06-09 2003-01-14 カーネギー メロン ユニヴァーシティ 反応性蛍光試薬としてのｐＨ官能性シアニン染料
JP2004315501A (ja) * 2003-03-28 2004-11-11 Tetsuo Nagano 亜鉛蛍光プローブ

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KAZUTAKA KIYOSE ET AL.: "Tricarbocyanine Kokkaku o Yusuru Ratio-gata Keiko Probe no Kaihatsu", DAI 126 KAI THE PHARMACEUTICAL SOCIETY OF JAPAN SENDAI2006 YOSHISHU 2, vol. 126 th, no. 2, 6 March 2006 (2006-03-06), pages 5 *
KIYOSE K. ET AL.: "Development of a Ratiometric Fluorescent Zinc Ion Probe in Near-Infrared Region, Based on Tricarbocyanine Chromophore.", J. AM. CHEM. SOC., vol. 128, no. 20, 24 May 2006 (2006-05-24), pages 6548 - 6549, XP003016875 *
KOJIMA H. ET AL.: "Development of Near-Infrared Fluorescent Probes for Nitric Oxide and Zinc Ion.", PROC. SPIE. INT. SOC. OPT. ENG., vol. 6441, January 2007 (2007-01-01), pages 64411P.1 - 64411P.12, XP055351773 *
SASAKI E. ET AL.: "Highly Sensitive Near- Infrared Fluorescent Probes for Nitric Oxide and Their Application to Isolated Organs.", J. AM. CHEM. SOC., vol. 127, no. 11, 23 March 2005 (2005-03-23), pages 3684 - 3685, XP055351772 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009107769A1 (ja) * 2008-02-29 2009-09-03 国立大学法人東京大学 活性酸素測定用試薬
JPWO2009107769A1 (ja) * 2008-02-29 2011-07-07 国立大学法人 東京大学 活性酸素測定用試薬
US20110313129A1 (en) * 2009-02-11 2011-12-22 Life Technologies Corporation Large stokes shift dyes
US9624424B2 (en) 2009-02-11 2017-04-18 Life Technologies Corporation Large stokes shift dyes
WO2011105610A1 (ja) * 2010-02-26 2011-09-01 国立大学法人名古屋大学 インスレーター及びその利用

Also Published As

Publication number Publication date
US20110117666A1 (en) 2011-05-19
JPWO2008108074A1 (ja) 2010-06-10
JP5559529B2 (ja) 2014-07-23
US8465985B2 (en) 2013-06-18

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