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WO2008107782A3 - Hydroxylase modifiée et ses applications - Google Patents

Hydroxylase modifiée et ses applications Download PDF

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Publication number
WO2008107782A3
WO2008107782A3 PCT/IB2008/000524 IB2008000524W WO2008107782A3 WO 2008107782 A3 WO2008107782 A3 WO 2008107782A3 IB 2008000524 W IB2008000524 W IB 2008000524W WO 2008107782 A3 WO2008107782 A3 WO 2008107782A3
Authority
WO
WIPO (PCT)
Prior art keywords
threonine
hydroxylase
applications
phenylacetyl
valine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2008/000524
Other languages
English (en)
Other versions
WO2008107782A2 (fr
Inventor
Micheal Durairaaj
Twinkle Jasmine Masilamani
Ravi Kanth Harit
Cavery Manian Krishnan
Ramanan Thirumoorthy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Orchid Pharma Ltd
Original Assignee
Orchid Chemicals and Pharmaceuticals Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orchid Chemicals and Pharmaceuticals Ltd filed Critical Orchid Chemicals and Pharmaceuticals Ltd
Publication of WO2008107782A2 publication Critical patent/WO2008107782A2/fr
Publication of WO2008107782A3 publication Critical patent/WO2008107782A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne une hydroxylase mutante ayant une activité accrue et une plus grande spécificité de substrat pour le phénylacétyl-7-ADCA pour la production de phénylacétyl-désacétyl-7-ACA, qui porte une ou plusieurs modifications des acides aminés dans les positions des résidus comparé à l'hydroxylase de type sauvage du groupe de résidus suivant : l'acide glutamique en position 16, la tyrosine en position 38, la proline en position 72, la thréonine en position 90, la valine en position 150, la proline en position 186, la valine en position 221, la méthionine en position 229, la thréonine en position 273, la thréonine en position 304 et l'alanine en position 311.
PCT/IB2008/000524 2007-03-06 2008-03-07 Hydroxylase modifiée et ses applications Ceased WO2008107782A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN445/CHE/2007 2007-03-06
IN445CH2007 2007-03-06

Publications (2)

Publication Number Publication Date
WO2008107782A2 WO2008107782A2 (fr) 2008-09-12
WO2008107782A3 true WO2008107782A3 (fr) 2008-11-13

Family

ID=39738872

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/000524 Ceased WO2008107782A2 (fr) 2007-03-06 2008-03-07 Hydroxylase modifiée et ses applications

Country Status (1)

Country Link
WO (1) WO2008107782A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104364376A (zh) * 2012-01-10 2015-02-18 幽兰化学医药有限公司 突变的头孢菌素羟化酶及其在脱乙酰基头孢菌烷酸合成中的应用

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107557348B (zh) * 2016-07-01 2022-07-08 百瑞全球有限公司 头孢菌素羟化酶突变体、编码该突变体的dna、使用该突变体的方法及其应用
CN114540320B (zh) * 2022-03-28 2024-01-26 上海光玥生物科技有限公司 胞苷羟化酶及其基因、用途和新冠口服药Molnupiravir的合成方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001085951A1 (fr) * 2000-05-09 2001-11-15 Acs Dobfar Uk Limited Expandase modifiee et utilisations
WO2007023369A1 (fr) * 2005-08-25 2007-03-01 Orchid Chemicals & Pharmaceuticals Limited Expandase-hydroxylase modifiee et applications correspondantes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001085951A1 (fr) * 2000-05-09 2001-11-15 Acs Dobfar Uk Limited Expandase modifiee et utilisations
WO2007023369A1 (fr) * 2005-08-25 2007-03-01 Orchid Chemicals & Pharmaceuticals Limited Expandase-hydroxylase modifiee et applications correspondantes

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104364376A (zh) * 2012-01-10 2015-02-18 幽兰化学医药有限公司 突变的头孢菌素羟化酶及其在脱乙酰基头孢菌烷酸合成中的应用
CN104364376B (zh) * 2012-01-10 2017-06-13 幽兰化学医药有限公司 突变的头孢菌素羟化酶及其在脱乙酰基头孢菌烷酸合成中的应用

Also Published As

Publication number Publication date
WO2008107782A2 (fr) 2008-09-12

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