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WO2008157689A3 - Methods of nucleic acid amplification analysis - Google Patents

Methods of nucleic acid amplification analysis Download PDF

Info

Publication number
WO2008157689A3
WO2008157689A3 PCT/US2008/067496 US2008067496W WO2008157689A3 WO 2008157689 A3 WO2008157689 A3 WO 2008157689A3 US 2008067496 W US2008067496 W US 2008067496W WO 2008157689 A3 WO2008157689 A3 WO 2008157689A3
Authority
WO
WIPO (PCT)
Prior art keywords
rtd
pcr
amplification
nucleic acid
reactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/067496
Other languages
French (fr)
Other versions
WO2008157689A9 (en
WO2008157689A2 (en
Inventor
Alexander M Chagovetz
James P Keener
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Utah Research Foundation Inc
Original Assignee
University of Utah Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Utah Research Foundation Inc filed Critical University of Utah Research Foundation Inc
Publication of WO2008157689A2 publication Critical patent/WO2008157689A2/en
Publication of WO2008157689A9 publication Critical patent/WO2008157689A9/en
Publication of WO2008157689A3 publication Critical patent/WO2008157689A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for quantifying nucleic acid sequences in amplification or reverse transcription reactions, involving the use of real-time detection (RTD) polymerase chain reaction (PCR) systems. The invention includes formulating mathematical models, based on kinetic descriptions of PCR for different RTD systems, and development of application algorithms for these models to quantitatively analyze RTD-PCR experiments. The invention also includes quantitative analysis of the effects of non-specific (secondary reactions) amplification on the efficiency of specific target amplification. The invention also includes quantitative analysis of multiple specific concurrent amplifications (multiplex reactions) including internal control relative quantification experiments.
PCT/US2008/067496 2007-06-19 2008-06-19 Methods of nucleic acid amplification analysis Ceased WO2008157689A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82065207A 2007-06-19 2007-06-19
US11/820,652 2007-06-19

Publications (3)

Publication Number Publication Date
WO2008157689A2 WO2008157689A2 (en) 2008-12-24
WO2008157689A9 WO2008157689A9 (en) 2009-03-12
WO2008157689A3 true WO2008157689A3 (en) 2009-07-09

Family

ID=40156966

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/067496 Ceased WO2008157689A2 (en) 2007-06-19 2008-06-19 Methods of nucleic acid amplification analysis

Country Status (1)

Country Link
WO (1) WO2008157689A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012255142B2 (en) 2011-05-18 2015-03-05 Diasorin Italia S.P.A. Systems and methods for valving on a sample processing device
KR20140022399A (en) 2011-05-18 2014-02-24 쓰리엠 이노베이티브 프로퍼티즈 컴파니 Systems and methods for volumetric metering on a sample processing device
EP2709762B1 (en) 2011-05-18 2021-03-31 DiaSorin S.p.A. Systems and methods for detecting the presence of a selected volume of material in a sample processing device
WO2014186526A2 (en) * 2013-05-15 2014-11-20 Thorne Diagnostics, Inc. Nucleic acid amplification signal acquisition and signal analysis

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5720923A (en) * 1993-07-28 1998-02-24 The Perkin-Elmer Corporation Nucleic acid amplification reaction apparatus
US7097973B1 (en) * 1999-06-14 2006-08-29 Alpha Mos Method for monitoring molecular species within a medium

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5720923A (en) * 1993-07-28 1998-02-24 The Perkin-Elmer Corporation Nucleic acid amplification reaction apparatus
US7097973B1 (en) * 1999-06-14 2006-08-29 Alpha Mos Method for monitoring molecular species within a medium
US7115229B2 (en) * 1999-06-14 2006-10-03 Alpha Mos Apparatus and method for monitoring molecular species within a medium

Also Published As

Publication number Publication date
WO2008157689A9 (en) 2009-03-12
WO2008157689A2 (en) 2008-12-24

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