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WO2008153223A1 - Composition permettant de mesurer l'affinité de liaison entre un acide nucléique et une substance et utilisation correspondante - Google Patents

Composition permettant de mesurer l'affinité de liaison entre un acide nucléique et une substance et utilisation correspondante Download PDF

Info

Publication number
WO2008153223A1
WO2008153223A1 PCT/JP2008/063705 JP2008063705W WO2008153223A1 WO 2008153223 A1 WO2008153223 A1 WO 2008153223A1 JP 2008063705 W JP2008063705 W JP 2008063705W WO 2008153223 A1 WO2008153223 A1 WO 2008153223A1
Authority
WO
WIPO (PCT)
Prior art keywords
substance
nucleic acid
binding affinity
measuring
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/063705
Other languages
English (en)
Japanese (ja)
Inventor
Kazuhiko Nakatani
Jinhua Zhang
Shiori Umemoto
Shinichi Sasaoka
Takahiro Wazaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nitto Kasei Co Ltd
University of Osaka NUC
Original Assignee
Nitto Kasei Co Ltd
Osaka University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nitto Kasei Co Ltd, Osaka University NUC filed Critical Nitto Kasei Co Ltd
Priority to US12/452,878 priority Critical patent/US20110263041A1/en
Priority to JP2009519347A priority patent/JP4533981B2/ja
Priority to CA2694030A priority patent/CA2694030A1/fr
Priority to EP08791936A priority patent/EP2175275A4/fr
Publication of WO2008153223A1 publication Critical patent/WO2008153223A1/fr
Anticipated expiration legal-status Critical
Priority to US13/484,505 priority patent/US8741659B2/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Cette invention concerne une composition permettant de mesurer l'affinité de liaison entre un acide nucléique et une substance présentant un intérêt et contenant un phosphore organique capable de se lier à l'ARN et présentant une intensité de fluorescence plus élevée dans sa forme non liée à l'ARN par rapport l'intensité de fluorescence dans sa forme liée à l'ARN. Il est alors possible de mesurer l'affinité de liaison entre une substance présentant un interêt et un acide nucléique de manière précise et simple. Il est également possible d'utiliser diverses substances en tant que substance devant être testée.
PCT/JP2008/063705 2007-07-31 2008-07-30 Composition permettant de mesurer l'affinité de liaison entre un acide nucléique et une substance et utilisation correspondante Ceased WO2008153223A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US12/452,878 US20110263041A1 (en) 2007-07-31 2008-07-30 Composition for measuring the binding affinity between nucleic acid and test substance, and use thereof
JP2009519347A JP4533981B2 (ja) 2007-07-31 2008-07-30 核酸と被験物質との結合親和性を測定するための組成物及びその利用
CA2694030A CA2694030A1 (fr) 2007-07-31 2008-07-30 Composition permettant de mesurer l'affinite de liaison entre un acide nucleique et une substance et utilisation correspondante
EP08791936A EP2175275A4 (fr) 2007-07-31 2008-07-30 Composition permettant de mesurer l'affinite de liaison entre un acide nucleique et une substance et utilisation correspondante
US13/484,505 US8741659B2 (en) 2007-07-31 2012-05-31 Composition for measuring the binding affinity between nucleic acid and test substance, and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007199970 2007-07-31
JP2007-199970 2007-07-31

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/452,878 A-371-Of-International US20110263041A1 (en) 2007-07-31 2008-07-30 Composition for measuring the binding affinity between nucleic acid and test substance, and use thereof
US13/484,505 Division US8741659B2 (en) 2007-07-31 2012-05-31 Composition for measuring the binding affinity between nucleic acid and test substance, and use thereof

Publications (1)

Publication Number Publication Date
WO2008153223A1 true WO2008153223A1 (fr) 2008-12-18

Family

ID=40129817

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/063705 Ceased WO2008153223A1 (fr) 2007-07-31 2008-07-30 Composition permettant de mesurer l'affinité de liaison entre un acide nucléique et une substance et utilisation correspondante

Country Status (5)

Country Link
US (2) US20110263041A1 (fr)
EP (2) EP2175275A4 (fr)
JP (1) JP4533981B2 (fr)
CA (1) CA2694030A1 (fr)
WO (1) WO2008153223A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2175275A4 (fr) 2007-07-31 2010-09-01 Univ Osaka Composition permettant de mesurer l'affinite de liaison entre un acide nucleique et une substance et utilisation correspondante
US10464970B2 (en) 2013-10-23 2019-11-05 The United States Of America As Represented By The Secretary, Department Of Health And Human Services Compounds that bind to human immunodeficiency virus rev response element

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2568699B2 (ja) * 1989-07-14 1997-01-08 松下電器産業株式会社 免疫的検出方法
JP2002241397A (ja) * 2001-02-20 2002-08-28 Rikogaku Shinkokai 蛍光性蛋白質および薬物探索法
JP2003334078A (ja) * 1999-04-20 2003-11-25 National Institute Of Advanced Industrial & Technology 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242796A (en) 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
CA1340806C (fr) 1986-07-02 1999-11-02 James Merrill Prober Methode, systeme et reactifs pour le sequencage de l'adn
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5654442A (en) 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
JPH08505121A (ja) 1992-09-03 1996-06-04 アプライド バイオシステムズ，インコーポレイテッド 分子プローブとしての４，７−ジクロロフルオレセイン染料
JP3927630B2 (ja) 1996-09-27 2007-06-13 エーザイ・アール・アンド・ディー・マネジメント株式会社 ウイルス感染症の予防・治療剤
US6699661B1 (en) 1999-04-20 2004-03-02 Kankyo Engineering Co., Ltd. Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
US6448407B1 (en) 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
AU2002367810A1 (en) * 2002-03-28 2003-11-10 Rutgers, The State Of University Of New Jersey Bis-transition-metal-chelate-probes
US20050171079A1 (en) * 2004-02-04 2005-08-04 Schrimpf Michael R. Amino-substituted tricyclic derivatives and methods of use
US7365193B2 (en) * 2004-02-04 2008-04-29 Abbott Laboratories Amino-substituted tricyclic derivatives and methods of use
EP2175275A4 (fr) 2007-07-31 2010-09-01 Univ Osaka Composition permettant de mesurer l'affinite de liaison entre un acide nucleique et une substance et utilisation correspondante

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2568699B2 (ja) * 1989-07-14 1997-01-08 松下電器産業株式会社 免疫的検出方法
JP2003334078A (ja) * 1999-04-20 2003-11-25 National Institute Of Advanced Industrial & Technology 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法
JP2002241397A (ja) * 2001-02-20 2002-08-28 Rikogaku Shinkokai 蛍光性蛋白質および薬物探索法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
OH K.J ET AL.: "Excimer-Based Peptide Beacons: A Conventional Experimental Approach for Monitoring Polypeptide-Protein and Polypeptide-Oligonucleotide Interactions", J. AM. CHEM. SOC., vol. 128, no. 43, 2006, pages 14018 - 14019, XP008128279 *
PACE T.C.S. ET AL.: "Photophysics of Aminoxanthone Derivatives and Their Application as Binding Probes for DNA", PHOTOBIOCHEM. PHOTOBIOL., vol. 82, no. 1, 2006, pages 78 - 87, XP008069388 *
PAINTER G.R. ET AL.: "Initial Binding of 2'-Deoxynucleoside 5'-Triphosphates to Human Immunodeficiency Virus Type 1 Reverse Transcriptase", J. BIOL. CHEM., vol. 266, no. 29, 1991, pages 19362 - 19368, XP008128276 *

Also Published As

Publication number Publication date
US20110263041A1 (en) 2011-10-27
EP2482076A1 (fr) 2012-08-01
US8741659B2 (en) 2014-06-03
CA2694030A1 (fr) 2008-12-18
EP2175275A4 (fr) 2010-09-01
US20120238029A1 (en) 2012-09-20
JPWO2008153223A1 (ja) 2010-08-26
JP4533981B2 (ja) 2010-09-01
EP2175275A1 (fr) 2010-04-14

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