[go: up one dir, main page]

WO2008151386A8 - Megakaryocyte differentiation - Google Patents

Megakaryocyte differentiation Download PDF

Info

Publication number
WO2008151386A8
WO2008151386A8 PCT/AU2008/000861 AU2008000861W WO2008151386A8 WO 2008151386 A8 WO2008151386 A8 WO 2008151386A8 AU 2008000861 W AU2008000861 W AU 2008000861W WO 2008151386 A8 WO2008151386 A8 WO 2008151386A8
Authority
WO
WIPO (PCT)
Prior art keywords
scf
tpo
formation
under conditions
conditions sufficient
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/AU2008/000861
Other languages
French (fr)
Other versions
WO2008151386A1 (en
Inventor
Andrew Elefanty
Eduoard Stanley
Elizabeth Ng
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Australian Stem Cell Centre Ltd
Original Assignee
Australian Stem Cell Centre Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2007903224A external-priority patent/AU2007903224A0/en
Application filed by Australian Stem Cell Centre Ltd filed Critical Australian Stem Cell Centre Ltd
Publication of WO2008151386A1 publication Critical patent/WO2008151386A1/en
Publication of WO2008151386A8 publication Critical patent/WO2008151386A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0644—Platelets; Megakaryocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/145—Thrombopoietin [TPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides a method for generating megakaryocytes and/or megakaryocyte precursors from a population of embryonic stem cells (ESCs), the method comprising: (i) culturing ESCs in a serum-free, stromal/feeder cell-free medium for a time and under conditions sufficient for formation of mesoderm and/or mesendoderm; and (ii) differentiating the cells cultured in step (i) in a medium comprising thrombopoietin (TPO), stem cell factor (SCF) and interleukin 3 (IL-3), or any functional fragment, variant or mimetic of TPO, SCF and/or IL-3, for a time and under conditions sufficient for formation of megakaryocytes and/or megakaryocyte precursors.
PCT/AU2008/000861 2007-06-15 2008-06-13 Megakaryocyte differentiation Ceased WO2008151386A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2007903224A AU2007903224A0 (en) 2007-06-15 Megakaryocyte differentiation
AU2007903224 2007-06-15

Publications (2)

Publication Number Publication Date
WO2008151386A1 WO2008151386A1 (en) 2008-12-18
WO2008151386A8 true WO2008151386A8 (en) 2009-02-05

Family

ID=40129148

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2008/000861 Ceased WO2008151386A1 (en) 2007-06-15 2008-06-13 Megakaryocyte differentiation

Country Status (1)

Country Link
WO (1) WO2008151386A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9938500B2 (en) 2006-04-14 2018-04-10 Astellas Institute For Regenerative Medicine Hemangio-colony forming cells

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190000928A (en) 2008-05-06 2019-01-03 아스텔라스 인스티튜트 포 리제너러티브 메디슨 Methods for producing enucleated erythroid cells derived from pluripotent stem cells
WO2009137624A2 (en) 2008-05-06 2009-11-12 Advanced Cell Technology, Inc. Hemangio colony forming cells and non-engrafting hemangio cells
CN101864396B (en) * 2010-05-17 2012-08-22 中国人民解放军军事医学科学院野战输血研究所 Method for inducing megakaryoblast and megakaryocyte in vitro
CA2815426A1 (en) * 2010-10-25 2012-05-10 The Children's Hospital Of Philadelphia Compositions and methods for the generation of platelets and methods of use thereof
PL2785359T3 (en) 2011-11-30 2018-12-31 Astellas Institute For Regenerative Medicine MEZENCHYMAL RIGID CELLS AND RELATED APPLICATIONS
US8961956B2 (en) 2011-11-30 2015-02-24 Ocata Therapeutics, Inc. Mesenchymal stromal cells and uses related thereto
GB201210857D0 (en) 2012-06-19 2012-08-01 Cambridge Entpr Ltd Transcription factor mediated programming towards megakaryocytes
WO2014011407A2 (en) 2012-07-12 2014-01-16 Imstem Biotechnology, Inc. Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof
CA2896053A1 (en) 2012-12-21 2014-06-26 Ocata Therapeutics, Inc. Methods for production of platelets from pluripotent stem cells and compositions thereof
ES2536605B1 (en) * 2013-10-24 2016-03-02 Fundación Pública Andaluza Progreso Y Salud Method of obtaining megakaryocytes and platelets
CN105749252B (en) * 2016-04-29 2025-11-14 南方医科大学 Application of IL-9 as a drug for treating thrombocytopenia
US11713447B2 (en) 2016-08-04 2023-08-01 Icahn School Of Medicine At Mount Sinai Production of megakaryoctye compositions and therapies for treatment of thrombocytopenia
CN113884682B (en) * 2021-04-30 2023-07-21 中国医学科学院血液病医院(中国医学科学院血液学研究所) Use of the product for detecting megakaryocyte or platelet surface marker molecules in the preparation of products for detecting infection

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070141703A1 (en) * 2003-11-19 2007-06-21 Stanley Edouard G Methods for producing blood products from pluripotent cells in cell culture
JP2008518603A (en) * 2004-11-01 2008-06-05 ウイスコンシン アラムニ リサーチ ファンデーション Stem cell-derived platelets

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9938500B2 (en) 2006-04-14 2018-04-10 Astellas Institute For Regenerative Medicine Hemangio-colony forming cells

Also Published As

Publication number Publication date
WO2008151386A1 (en) 2008-12-18

Similar Documents

Publication Publication Date Title
WO2008151386A8 (en) Megakaryocyte differentiation
WO2007002210A3 (en) Embryonic stem cell culture compositions and methods of use thereof
WO2007002385A3 (en) Mesoderm and definitive endoderm cell populations
SG152273A1 (en) Pdx1 expressing endoderm
WO2006020919A3 (en) Compositions and methods for self-renewal and differentiation in human embryonic stem cells
EP4368701A3 (en) Feeder-free culture system
WO2006083782A3 (en) Directed differentiation of embryonic stem cells and uses thereof
GB2451523B (en) Pluripotent cells from rat and other species
NZ592622A (en) Stem cell aggregate suspension compositions and methods for differentiation thereof
MX343462B (en) Cell culture medium.
WO2006070370A3 (en) Stem cells culture systems
WO2006130651A3 (en) Method of forming dendritic cells from embryonic stem cells
WO2008129563A3 (en) Human mesenchymal stem cells and preparation thereof
MY185887A (en) Cell culture improvements
SG10201901417UA (en) Production of proteins in glutamine-free cell culture media
GB2449042A (en) Erythroid cells producing adult-type b-hemoglobin generated from human embryonic stem cells
EP2896690A3 (en) Use of RNA for reprogramming somatic cells
EP1860180A4 (en) METHOD FOR IN VITRO AMPLIFICATION OF ENDOTHELIAL PROGENITOR CELL
WO2008091830A3 (en) Maintenance and propagation of mesenchymal stem cells
WO2008060541A3 (en) In vitro expansion of postpartum-derived cells using microcarriers
WO2011011349A3 (en) Differentiation of human embryonic stem cells
Yasuda et al. NANOG maintains self‐renewal of primate ES cells in the absence of a feeder layer
WO2008063675A3 (en) Endodermal progenitor cells
WO2008067142A3 (en) In vitro differentiation of hematopoietic cells from primate embryonic stem cells
WO2003100010A3 (en) Cytokine-free growth and maintenance of progenitor cells

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08756941

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase in:

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08756941

Country of ref document: EP

Kind code of ref document: A1