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WO2008150937A3 - Real-time pcr - Google Patents

Real-time pcr Download PDF

Info

Publication number
WO2008150937A3
WO2008150937A3 PCT/US2008/065181 US2008065181W WO2008150937A3 WO 2008150937 A3 WO2008150937 A3 WO 2008150937A3 US 2008065181 W US2008065181 W US 2008065181W WO 2008150937 A3 WO2008150937 A3 WO 2008150937A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
real
time pcr
rna
expression level
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/065181
Other languages
French (fr)
Other versions
WO2008150937A2 (en
Inventor
Mark G. Erlander
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AIVARADX Inc
Original Assignee
AIVARADX Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AIVARADX Inc filed Critical AIVARADX Inc
Publication of WO2008150937A2 publication Critical patent/WO2008150937A2/en
Anticipated expiration legal-status Critical
Publication of WO2008150937A3 publication Critical patent/WO2008150937A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The disclosure provides for the use of real-time PCR to detect the expression level(s) of genetic material in a sample. The expression level of any gene sequence, in the form of messenger RNA (mRNA) molecules, may be detected and/or quantified. The disclosed methods may be applied to RNA profiling, or determining the expression levels of multiple gene sequences in a biological sample, including a sample containing relatively degraded RNA, such as an FFPE sample.
PCT/US2008/065181 2007-05-29 2008-05-29 Real-time pcr Ceased WO2008150937A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94068607P 2007-05-29 2007-05-29
US60/940,686 2007-05-29

Publications (2)

Publication Number Publication Date
WO2008150937A2 WO2008150937A2 (en) 2008-12-11
WO2008150937A3 true WO2008150937A3 (en) 2009-12-30

Family

ID=40094360

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/065181 Ceased WO2008150937A2 (en) 2007-05-29 2008-05-29 Real-time pcr

Country Status (1)

Country Link
WO (1) WO2008150937A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2256215A1 (en) * 2009-04-30 2010-12-01 Steffen Mergemeier Assay system using a nuclease activity of a nucleic acid polymerase
EP2744916A4 (en) 2011-07-13 2015-06-17 Primeradx Inc MULTIMODAL METHODS FOR SIMULTANEOUS DETECTION AND QUANTIFICATION OF MULTIPLE NUCLEIC ACIDS IN A SAMPLE

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050053942A1 (en) * 2002-06-24 2005-03-10 Sakari Kauppinen Methods and systems for detection and isolation of a nucleotide sequence
US20070065846A1 (en) * 2002-03-13 2007-03-22 Baker Joffre B Gene expression profiling in biopsied tumor tissues

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070065846A1 (en) * 2002-03-13 2007-03-22 Baker Joffre B Gene expression profiling in biopsied tumor tissues
US20050053942A1 (en) * 2002-06-24 2005-03-10 Sakari Kauppinen Methods and systems for detection and isolation of a nucleotide sequence

Also Published As

Publication number Publication date
WO2008150937A2 (en) 2008-12-11

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