[go: up one dir, main page]

WO2008146649A1 - 酵素含有ゲル及び核酸の増幅キット - Google Patents

酵素含有ゲル及び核酸の増幅キット Download PDF

Info

Publication number
WO2008146649A1
WO2008146649A1 PCT/JP2008/059183 JP2008059183W WO2008146649A1 WO 2008146649 A1 WO2008146649 A1 WO 2008146649A1 JP 2008059183 W JP2008059183 W JP 2008059183W WO 2008146649 A1 WO2008146649 A1 WO 2008146649A1
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
nucleic acid
containing gel
acid amplification
amplification kit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/059183
Other languages
English (en)
French (fr)
Inventor
Ken Takubo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp filed Critical Olympus Corp
Priority to JP2009516257A priority Critical patent/JPWO2008146649A1/ja
Publication of WO2008146649A1 publication Critical patent/WO2008146649A1/ja
Priority to US12/625,469 priority patent/US20100081175A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

本発明は、タンパク質と核酸を含有する試料から、容易かつ簡便に、タンパク質を失活させて該試料中の核酸を増幅するために用いられる酵素含有ゲル、該酵素含有ゲルを有する核酸の増幅キット、及び該酵素含有ゲルを用いた核酸の増幅方法を提供することを課題とする。本発明は、耐熱性酵素を含有することを特徴とする酵素含有ゲル、前記酵素含有ゲルとタンパク質分解酵素とを有する核酸の増幅キット、前記酵素含有ゲルを用いる核酸の増幅方法を提供する。
PCT/JP2008/059183 2007-05-25 2008-05-20 酵素含有ゲル及び核酸の増幅キット Ceased WO2008146649A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2009516257A JPWO2008146649A1 (ja) 2007-05-25 2008-05-20 酵素含有ゲル及び核酸の増幅キット
US12/625,469 US20100081175A1 (en) 2007-05-25 2009-11-24 Enzyme-containing gels and nucleic acid amplifying kits

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-139144 2007-05-25
JP2007139144 2007-05-25

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/625,469 Continuation US20100081175A1 (en) 2007-05-25 2009-11-24 Enzyme-containing gels and nucleic acid amplifying kits

Publications (1)

Publication Number Publication Date
WO2008146649A1 true WO2008146649A1 (ja) 2008-12-04

Family

ID=40074913

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/059183 Ceased WO2008146649A1 (ja) 2007-05-25 2008-05-20 酵素含有ゲル及び核酸の増幅キット

Country Status (3)

Country Link
US (1) US20100081175A1 (ja)
JP (1) JPWO2008146649A1 (ja)
WO (1) WO2008146649A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023506338A (ja) * 2019-12-11 2023-02-16 イルミナ インコーポレイテッド フローセルへの固定化
JP2024036617A (ja) * 2020-01-31 2024-03-15 株式会社島津製作所 病原体検出方法およびキット

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019009592A2 (ko) * 2017-07-04 2019-01-10 한국과학기술연구원 Ucst 소재를 적용한 입자 및 이를 이용한 핵산 증폭 방법
CN115141877A (zh) * 2021-03-30 2022-10-04 杭州杰毅生物技术有限公司 一种核酸等温扩增方法及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
EP0515506B1 (en) * 1990-02-16 2000-01-05 F. Hoffmann-La Roche Ag Improvements in the specificity and convenience of the polymerase chain reaction
US5840305A (en) * 1996-03-14 1998-11-24 The Picower Institute For Medical Research Treatment of HIV-Infection by interfering with host cell cyclophilin receptor activity

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BLAIR P. ET AL.: "Wax-embedded PCR reagents", PCR METHODS AND APPLICATIONS, vol. 4, no. 3, 1994, pages 191 - 194, XP000484537 *
KAGAKU JITEN: "Tokyo Kagaku Dojin", vol. 1ST ED., 1994, pages: 439 *
THE JAPANESE BIOCHEMICAL SOCIETY: "Shin Seikagaku Jikken Koza 1 Tanpakushitsu II -Ichiji Kozo-", vol. 1ST ED., 1990, TOKYO KAGAKU DOJIN, pages: 255 - 256 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023506338A (ja) * 2019-12-11 2023-02-16 イルミナ インコーポレイテッド フローセルへの固定化
JP7737309B2 (ja) 2019-12-11 2025-09-10 イルミナ インコーポレイテッド フローセルへの固定化
JP2024036617A (ja) * 2020-01-31 2024-03-15 株式会社島津製作所 病原体検出方法およびキット

Also Published As

Publication number Publication date
US20100081175A1 (en) 2010-04-01
JPWO2008146649A1 (ja) 2010-08-19

Similar Documents

Publication Publication Date Title
WO2006043178A3 (en) Enzymes
GB2446525A (en) Stem cell culture medium and method
DK1680516T3 (da) Ruminddelt autonom mærkning
WO2007100934A3 (en) Methods and compositions for the rapid isolation of small rna molecules
WO2006020767A3 (en) Thiazole based inhibitors of atp-utilizing enzymes
AU2003241311A1 (en) Simple catalytic dna biosensors for ions based on color changes
WO2005047511A3 (en) Bacterial expression of protease inhibitors and variants thereof
EP1952469A4 (en) DIRECT TRANSFER OF ELECTRONS USING ENZYMES IN BIOANODES, BIOCATHODS AND BIOCULTURABLE BATTERIES
WO2009158627A3 (en) Cellulosic protein expression in yeast
ATE450620T1 (de) Stabiler kreatinin-biosensor mit drei enzymen
WO2005087951A3 (en) Reagents, methods and kits for use in deactivating nucleic acids
WO2008146649A1 (ja) 酵素含有ゲル及び核酸の増幅キット
WO2004048935A3 (en) Detection of protease enzymes
WO2008034622A3 (en) A method of detecting and/or quantifying expression of a target protein candidate in a cell, and a method of identifying a target protein of a small molecule modulator
WO2005047462A3 (en) Universal control for nucleic acid amplification
WO2005028680A3 (en) Methods, compositions, and kits for the concentration and detection of microorganisms
WO2006025983A3 (en) Labeled substrate conjugates for identifying enzyme inhibitors
WO2006029389A9 (en) Tryptophan-processing enzymes, nucleic acids encoding them and methods for making and using them
WO2006010930A3 (en) Enzymes
WO2007140004A3 (en) Primers for use in detecting metallo-beta-lactamases
WO2006063116A3 (en) Bifunctional enzyme with ϝ-glutamyleysteine synthetase and glutathione synthetase activity and uses thereof
WO2004020970A3 (en) α-AMYLASE ASSAY AND USES THEREOF
TW200708732A (en) Water-miscible conductive ink for use in enzymatic electrochemical-based sensors
WO2005122709A3 (en) Purification methods and uses thereof
WO2005024045A3 (en) Primers for use in detecting beta-lactamases

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08752979

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2009516257

Country of ref document: JP

Kind code of ref document: A

122 Ep: pct application non-entry in european phase

Ref document number: 08752979

Country of ref document: EP

Kind code of ref document: A1