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WO2008144052A3 - Bioluminescent imaging of stem cells - Google Patents

Bioluminescent imaging of stem cells Download PDF

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Publication number
WO2008144052A3
WO2008144052A3 PCT/US2008/006429 US2008006429W WO2008144052A3 WO 2008144052 A3 WO2008144052 A3 WO 2008144052A3 US 2008006429 W US2008006429 W US 2008006429W WO 2008144052 A3 WO2008144052 A3 WO 2008144052A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
bioluminescent imaging
mammal
methods
stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/006429
Other languages
French (fr)
Other versions
WO2008144052A2 (en
Inventor
Rampyari Walia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2008144052A2 publication Critical patent/WO2008144052A2/en
Publication of WO2008144052A3 publication Critical patent/WO2008144052A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A61K49/0045—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent agent being a peptide or protein used for imaging or diagnosis in vivo
    • A61K49/0047—Green fluorescent protein [GFP]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A61K49/0045—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent agent being a peptide or protein used for imaging or diagnosis in vivo
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0063—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres
    • A61K49/0069—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres the agent being in a particular physical galenical form
    • A61K49/0097—Cells, viruses, ghosts, red blood cells, viral vectors, used for imaging or diagnosis in vivo

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods and compositions for detecting and localizing light originating from cultured stem cells or stem cells injected into tissue or an animal, especially a mammal, are described. Also disclosed are methods for localization of stem cells in selected regions, as well as for tracking stem cells within the mammal, and for causing stem cell differentiation.
PCT/US2008/006429 2007-05-18 2008-05-19 Bioluminescent imaging of stem cells Ceased WO2008144052A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93904607P 2007-05-18 2007-05-18
US60/939,046 2007-05-18

Publications (2)

Publication Number Publication Date
WO2008144052A2 WO2008144052A2 (en) 2008-11-27
WO2008144052A3 true WO2008144052A3 (en) 2009-02-05

Family

ID=40122249

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/006429 Ceased WO2008144052A2 (en) 2007-05-18 2008-05-19 Bioluminescent imaging of stem cells

Country Status (1)

Country Link
WO (1) WO2008144052A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9719146B2 (en) 2009-09-09 2017-08-01 General Electric Company Composition and method for imaging stem cells
CN102517386B (en) * 2011-12-15 2014-02-12 浙江省农业科学院 A lentiviral T vector for promoter analysis and its construction method and application
US9719127B2 (en) 2012-03-20 2017-08-01 The Regents Of The University Of California Dual inducible vectors and cell lines
CN102978164B (en) * 2012-11-14 2014-12-10 中国人民解放军第三军医大学第一附属医院 Channelrhodopsin-2 (ChR2)-green fluorescence protein (GFP) gene engineered nerve stem cell line and construction method thereof
US20210364497A1 (en) * 2018-04-02 2021-11-25 University Of Miami IFN-beta Reporter System for Primary Cells
EP3976116A4 (en) 2019-05-30 2023-08-23 490 Biotech, Inc. Lux expression in cells and methods of use
CN116703909B (en) * 2023-08-07 2023-10-27 威海海泰电子有限公司 Intelligent detection method for production quality of power adapter

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5635380A (en) * 1994-01-18 1997-06-03 Vanderbilt University Enhancement of nucleic acid transfer by coupling virus to nucleic acid via lipids
US20020073441A1 (en) * 2000-12-13 2002-06-13 Ross Brian D. Compositions and methods for detecting proteolytic activity
US20060035373A1 (en) * 2002-11-01 2006-02-16 Hongbing Zhang Stem cell libraries
WO2006096735A2 (en) * 2005-03-07 2006-09-14 New England Biolabs Inc Enhancing a luminescent signal

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5635380A (en) * 1994-01-18 1997-06-03 Vanderbilt University Enhancement of nucleic acid transfer by coupling virus to nucleic acid via lipids
US20020073441A1 (en) * 2000-12-13 2002-06-13 Ross Brian D. Compositions and methods for detecting proteolytic activity
US20060035373A1 (en) * 2002-11-01 2006-02-16 Hongbing Zhang Stem cell libraries
WO2006096735A2 (en) * 2005-03-07 2006-09-14 New England Biolabs Inc Enhancing a luminescent signal

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
AN ET AL.: "Efficient Lentiviral Vectors for Short Hairpin RNA Delivery into Human cells", HUMAN GENE THERAPY, vol. 14, 10 August 2003 (2003-08-10), pages 1207 - 1212 *
CONTAG ET AL.: "ADVANCES IN IN VIVO BIOLUMINESCENCE IMAGING OF GENE EXPRESSION", ANNU. REV. BIOMED. ENG., vol. 4, 2002, pages 235 - 260 *
CONTAG ET AL.: "Visualizing Gene Expression in Living Mammals Using a Bioluminescent Reporter", PHOTOCHEMISTRY AND PHOTOBIOLOGY, vol. 66, no. 4, 1997, pages 523 - 531 *
GIANNOLA ET AL.: "Hematopoietic Expression of HOXB4 is Regulated in Normal and Leukemic Stem Cells through Transcriptional Activation of the HOXB4 Promoter by Upstream Stimulating Factor (USF)-1 and USF-2", J. EXP. MED., vol. 192, no. 10, 20 November 2000 (2000-11-20), pages 1479 - 1490 *
LIU ET AL.: "Secretion of functional Renilla reniformis luciferase by mammalian cells", GENE, vol. 203, 1997, pages 141 - 148 *
MARKOVA ET AL.: "Cloning and Expression of cDNA for a Luciferase from the Marine Copepod Metridia longa", THE JOURNAL OF BIOLOGICAL CHEMISTRY, 30 January 2004 (2004-01-30) *
ROMAN ET AL.: "Analysis of progenitor cell scaffold combinations by in vivo non-invasive photonic imaging", BIOMATERIALS, vol. 28, 20 February 2007 (2007-02-20), pages 2718 - 2728 *
SHAH ET AL.: "Glioma Therapy and Real-Time Imaging of Neural Precursor Cell Migration and Tumor Regression", ANN. NEURO., vol. 57, 2005, pages 34 - 41 *
TANNOUS ET AL.: "Codon-Optimized Gaussia Luciferase cDNA for Mammalian Gene Expression in Culture and in Vivo", MOLECULAR THERAPY, vol. 11, no. 3, March 2005 (2005-03-01), pages 435 - 443 *
WAUD ET AL.: "Measurement of proteases using chemiluminescence-resonance-energy transfer chimaeras between green fluorescent protein and aequorin", BIOCHEM. J., vol. 357, 2001, pages 687 - 697 *

Also Published As

Publication number Publication date
WO2008144052A2 (en) 2008-11-27

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