WO2008143365A1

WO2008143365A1 - Composition containing chlorella and herbal extracts

Info

Publication number: WO2008143365A1
Application number: PCT/KP2008/000012
Authority: WO
Inventors: Sin Yong Rim
Original assignee: Individual
Current assignee: Individual
Priority date: 2007-05-18
Filing date: 2008-01-21
Publication date: 2008-11-27
Anticipated expiration: 2009-11-18
Also published as: CN101686722B; CN101686722A

Abstract

The present invention relates to a novel health food comprising chlorella extract, a herbal extract obtained from a mixture of herbs selected from Panax ginseng, Ganoderma lucidum, Valeriana frauriei birq, Poria cocus, Zerumbet Bupleurum scorzoneraefolium, Glycyrrhiza and along with a malt and honey. The health food has the enhancement effects of anti-oxidant, immunoenhancing, autonerve-modulating and hematopoiesis.

Description

COMPOSITION CONTAINING CHLORELLA AND HERBAL

Field of the Invention

The present invention relates to a novel health food comprising chlorella extract, a herbal extract obtained from a mixture of herbs selected from Panax ginseng, Ganoderma lucidum, Valeriana frauriei birq, Poria cocus, Zerumbet, Bupleurum scorzoneraefolium, Glycyrrhiza and along with a malt and honey. The novel health food has the enhancement effects of anti-oxidant, immunoenhancing, autonerve-modulating and hematopoiesis.

Background of the Invention

Chemical drugs, and health foods comprising the immune-enhancing factors derived from plants, for improving the immune system, have been developed in many countries. It has been known that the immune defence system is activated or suppressed by endogenous or exogenous factor. It appears that immune defence system is suppressed by many factors such as the oxidative stress, the immune cells dysfunction, the imbalance of autonerve system, hematopoiesis dysfunction, malnutrition and so on.

Oxidative stress is a stress, caused by free radicals like superoxide occurred by metabolism, various diseases, radioactive rays and the like.

Free radicals are natural by-products of daily metabolism and contribute to diseases and aging process. The presence of free radicals appears to contribute to suppression of the immune system and cells damage. Especially, it appears that the free radicals can suppress the immune cell, or macrophage, which plays essential and pivotal role in immune response. Antioxidants can counter the effects of free radicals, thereby enhancing immune function.

The amount of lymphocytes are reduced in humans with immunosupression disorders or likely diseases, and the cytokines are also reduced accordingly. Cytokines play the important role in the immune system.

Some of studies have also indicated that the immune system is related closely to the autonomic nerve system. It appears that, during stress, whether inflicted on the body through heavy exercise, severe illness or a viral infection, the sympathetic nerve system is excited while the parasympathetic nerve system is suppressed, thereby symptoms such as insomnia, sensibilities and digestive trouble may occur. The continual statue may cause decreasing the immune defence function.

It has been considered that the inhibition of the sympathetic nervous system may be used to enhance an immune response when the endogenous immune response is not sufficient, while sthenia of the parasympathetic nervous system may strengthen immune response. The immune defence function is enhanced by the immune stimulative, hematopoiesis accelarator and nutrition.

Various drugs have a good effect in human' immune system, but the herbal extract which controls anti-oxidant action, immunocell stimulation, enhance of hematopoiesis function and autonomic nerve system is not known.

The major object of the invention is to provide health food that enhances immune defense function to treat, or prevent various diseases.

The immunomodulators, hematopoiesis-related agents and nutritional supplement enhance immune defences.

The health food according to the present invention is prepared for the reduction of oxidation stress, activation of immunocyte, the control of autonomic nerve system and the enhance of hematopoiesis system.

The health food according to the present invention comprises Chlorella extract, herbal extract obtained from a mixture of herbs selected from Panax ginseng, Ganoderma Lucidum, Valeriana fauriei briq, Poria cocus, Zerumbet, Bupleurum scorzoneraefolium, Glycyrrhiza and along with malt and honey.

Brief Description of the invention

The chlorella, called hyperaliment, is a single cell green algae, which contains polysaccharide, polysaccharide mixture and active glucan and is used as immune modulation agent. The chlorella is also rich in vitamins, mineral, esculent fiber, nucleic acids and amino acids. Panax ginseng enhances immune response by stimulating immune cells which secretes cytocine. It promotes fatigue and sanguification preventing hemolyzation. It also has effect on sthenia, exiting systema nervosum, cardiac, sexual stimulation, digestion and absortion, as well as anti-allergy. It also has antioxidative effect.

Ganoderma lucidum contains ergosterol, protease, various amino acids, organic acids and polysacchrides. It is well kown for its effective treatment for the sedative action, spinal cord, prostate gland, autoimmunity diseases, hematopoiesis function, gastrointestinal tract, small bowel, colon and liver. The glucan and lentinan in ganoderma lucidum raise immune function and are used as antiblastic agent.

In many prior arts, polysccharides derived from chlorella, Panax ginseng, Ganoderma lucidum are known as immune cell-activating agent. This' invention is purposed to make the immune-stimulating activity of above-mentioned polysaccharide favorably toward the conditions of treatment for various disease.

Valeriana frauriei birq has a good indication to improve the palpitation, nervousness, agrypnia and heart disorers, which is known to be so by the effect of its lipid component.

Poria cocus contains large amount of pakaman belonging polysaccharide, which has aryypina and anetic action.

Zerumbet contains Volatile oils and materials like oil, by which it is useful for the treatment of stomach ache, vomiting, vomiting of pregnancy, indigestion as the aromatic, stomachic and antemetic drug.

Bupleurum scorzoneraefolium has antipyretic and sedative actions, by which it is useful for the of malaria, cold, intercostals neuralgia, nervous exhaustion, anxiety and soon and also the treatment of hepatitis, cholecystitis and cholepatia. It is known Such action

Glycyrrhiza has been used for treatment of other diseases as ulcer and productive cough, and for the detoxification and flavor since long time ago and glycyrrhizin is known as main component of such indiciation. The various mineral and perula acids contained in malts are noted for its distinct antioxidant activity and sympathetic nerve-depressing activity.

The present health food has shown wonderful treatment effect for diseases such as cerebrovascular disease, respiratory system disease, digestive systemdisease, urogenital system disease, metabolism disorders, hepatitis, tuberculosis, obstetrics and gynaecology diseases, autonomic neropathy and climacteric disorders.

Detailed description of the invention.

The present invention provides a health food comprising of: Chlorella extract, herbal extract obtained from amixture of herbs selected from Panax ginseng, Ganoderma Lucidum, Valeriana fauriei briq, Poria cocus, Zerumbet, Bupleurum scorzoneraefolium, Glycyrrhiza and along with malt and honey.

In an embodiment of the present invention, the content of chlorella extract is in the range about 5 mg to 50 mg, preferably 10 mg to 20 mg.

In an embodiment of the present invention, Panax ginseng is in the range about 14% to 17% of the health food.

In an embodiment of the present invention, Ganoderma Lucidum is in the range about 13% to 17% of the health food.

In an embodiment of the present invention, Valeriana fauriei briq is in the range about 12% to 18% of the health food.

In an embodiment of the present invention, Poria cocus is in the range about 10% to 15% of the health food.

In an embodiment of the present invention, Zerumbet is in the range about 10% to 13% of the health food.

In an embodiment of the present invention, Bupleurum scorzoneraefolium is in the range about 5% to 9% of the health food. In an embodiment of the present invention, Glycyrrhiza is in the range about 6% to 7% of the health food.

In one or more embodiment of the present invention, the percentage ratio of the malt is in the range of 8 to 10.

In another embodiment of the present invention, the health food provides antioxidant, autonerve-modulating, immune enhancing, and hematopoiesis properties.

The present invention also provides a process for preparing the health food which has antioxidant, autonerve-modulating, immune-enhancing, and hematopoiesis properties.

A detailed description of the present invention is provided in the Example section.

Example 1. Preparing of the composition according to the present Invention ,-,.;.

Preparing of the aqueous composition of the present invention. Chloralla extract; Dissolve the dried powder of chlorella in ethyl acetate, and extract at 45⁰C for 10 hr. Centrifuge (a)solution to obtain the supernant.

Add the ethanol (80% final ethanol content)into the supernant precipitate the active agents. Filter the precipitate, wash with absolute ethanol and dry the extracts under vacuum.

Herbs extract;

Mix and crush the Panax ginseng, Ganoderma lucidum, Valeriana fauriei briq, poria cocus,

Zerumbet, Bupleurum scorzoneraefolium, Glycyrrhiza with size of about 0.001-10mm³.

Add the crushed mixture into the distilled water, or ethanol and extract for 5h at 8O⁰C.

Centrifuge extracted solutions to obtain the supernant.

Malt;

Steam the rice bran, and then inoculate with Aspergillus oryzae.

Ferment at 20°C-25°C for 2 to 3 days,

Self-degradation at 50°C-80°C for about 10 hours, Add the distilled water into the Self -degraded solution, Centrifiige the mixed solution to obtain the supernatants.

Mixture; chlorella extracts, malt extracts and herbs extracts obtained from above steps mix and add the honey to composition of the present invention.

2. Preparing of the solid composition of the present invention.

1) Mix and crush the Panax ginseng, Ganoderma lucidum, Valeriana fauriei briq, poria cocus, Zerumbet, Bupleurum scorzoneraefolium, Glycyrrhiza,.

2) Add the crushed mixture into the distilled water, extract for 24h at 6O⁰C.

3) Extracted solution filter and concentrate,

4) mix the concentrated materials with the chlorella extracts and malt extracts and then malaxate with the suitable honey,

5) prepare the pill with the malaxated mixture

As above process, the compositions of the present invention and contents their compenents are shown tables 1, 2, 3 and.

Table.1 Invention composition 1

Table.2 Invention composition 2 12

-7-

*mg is chlorella extract add, not w/w.

Example 2. Measurement of anti-oxidant activity

Since elimination of DPPH free radicals changes the color of DPPH solution, anti-oxidant activity was measured, as follows;

DPPH of 5mg was dissolved in 100ml of 60% methanol to prepare a DPPH solution. Sample solution; mixed solution of the DPPH solution (750/^1) and the UC(2Oθ£tl). Blank solution; mixed solution of 60% methanol (750 μ.1) and UC solution (200 ^tI).

Control solution; mixed solution of the DPPH solution (750 μ, 1) and 60% methanol(200/xl).

Absorbance of each solution was measured at 515nm. Anti-oxidant activity (elimination activity of free radicals) was calculated from the following formula;

Anti-oxidant activity (%)=(C-S)/(C-B) X 100 C: Absorbance of the control B: Absorbance of the blank S: Absorbance of the sample

The above procedure was applied to invention composition 1, 2, 3 or 4 respectively. The antioxidant activities are shown in the following Table 5.

TableS : Anti-oxidant activity of present invention compositions

Invention composition 1 2 3 4 anti-oxidant activity (%) 82 87 83 85 As shown in the above table, the present invention compositions showed very high antioxidant activity.

Example 3. Measurement of hematopoiesis function

Radiation destroys bone marrow cells and has a negative effect on the generation and replication of normal immune cells, thereby deteriorating the function of hematopoiesis and immunization. In the present experiment, it has examined whether the administration of UC can reduce the deterioration of hematopoiesis and immune function accompanying radiation treatment.

After the mice weighing 24-27g were exposed to 2Gy of 60Co, gamma, 0.3ml/kg of UC 2 was orally administered to mice for 30 days. 10 days, 20 days and 3Od ays after irradiation, irrespectively, the mice were killed to harvest the peripheral blood to measure the number of erythrocyte(red blood cell:RBC), leucocytes (white blood celkWBC) and blood ρlatelet(BP).

Table 6: Variation of the number OfRBC(X 10¹²/ fi )(n=30)

Table 7; Variation of the number of BP(X 109/ fi )(n=30)

Table 8: Variation of the number of WBC(X 109/ fi )(n=30)

Treatment Pre - irradiation Post- irradiation (X±ms) group (X±ms) IOdays 20days 30days Control 11.61 ± 0.26(100.0) 8.9 ± 1.02(76.65) 11.76±0.65(113.18) 9.4 ±0.56(80.96)

UC2 10.39+0.22(100.0) 10.39+0.22*(110.58) 12.29+ 1.12*(124.15)10.58+0.39(101.82)

( ) indicates the relative ratio.

* indicates level of significance (p<0.05) compared to the control group. ** indicates level of significance (p<0.01) compared to the control group. Data represents mean ± standard error.

Example 4. Effect on the modulation of the autonerve system.

hi the present experiment, the effect of UC 1 to modulate the autonerve system is measured by the method of heart rate variability-check. ,

The patients are laid down on the bed for 5min to lOmin and then conductor electrocardiogram II (ECG II ) is set up on the patients's limb. The electrocardiogram data is input continuously to computer for 5min.

The ratios of LF/HF is reflected the function of sympathetic nerve system.

0.3ml/kg of the UC 2 were orally administrated to patients with insomnia, 2h after administration; LF/HF was measured according to above method.

Table 9. Effect on the modulation of the autonerve system(n=5)

As shown in the above table the present invention composition showed the effect on the invention of sympathetic nerve system and sthenia of the parasympathe tic nerve system.

Example5. Effect of the invention composition on phagocytosis of cells from peripheral blood.

A group of mice has been injected intraperitonealy with with PBS(control) or UC. 24 later, mice were scarified, peripheral blood from the orbital plexus was collected into heparine(5iv/ml).

After counting, a test of phagocytosis of yeast partides was performed.

0.1ml of heparinzed fresh blood was add to 0.05ml of diluted yeast particles(5*10⁸/ml) and incubated for 60 minutes at 37⁰C with occasional gentle agitation.

After the end of incubation, the cell suspension was smeared over microscope slides.

Smears were evaluated under the optical microscope after Accustain staining.

Cells with at least three engulfed particles were considered to be positive.

The results are given in the following tableό. the results clearly show that the invention composition strongly stimulate phagocytosis in both monocytes and granulocytes.

Table 10. percents of engulfed monocytes and granulocytes

Example 10. Effect of the invention composition on the engulfment of cells from peritoneal cavity

A group of mice has been injected intrapritonealy with PBS(control) or UC. 24 later, mice were scarified, peritoneal cells were collected into medium. After counting the cells in hemocytometer, the peritoneal cells were dilated to l*10⁷cells in medium with 5% fetal calf serum.

2* 10⁶ cells in 0.2ml of medium supplemented with 5% fetal calf serum were mixed with the same volume of yeast particles(5*10⁸/ml). The suspension was incubated for 60 minutes at 37⁰C with occasional agitation. Incubation was terminated by centrifugation (150g for 5minute) and the pellet was resupended. Macrophages with ingested particles were scored under the optical microscope in the smears after. Accustain staining. Cells with four or more engulfed particles were considered to be positive.

The results are given in the following table7. they show that the invention composition strongly stimulate phagocytosis of peritoneal macrophages.

Table 11. Percents of the engulfed macrophages

Claims

Claims:

1. A composition comprising of: a chlorella extract, a concentrated herbal extract obtained from a mixture of herbs selected from Panax ginseng, Ganoderma lucidum, Valeriana fauriei briq, Poria cocus, Zerumbet Bupleurum scorzoneraefolium, Glycyrrhiza and malt extract along with honey.

2. The composition food as claimed in claim 1, which comprises chlorella extract in an amount of about 5 mg to 50 mg, preferably 10 mg to 20 mg; Panax ginseng in an amount of about 14% to 17% , Ganoderma Lucidum in an amount of about 13% to 17% , Valeriana fauriei briq in an amount of about 12% to 18%, Poria cocus in an amount about 10% to 15%, Zerumbet in an amount of about 10% to 13%, Bupleurum scorzoneraefolium in an amount of about 5% to 9%, Glycyrrhiza in the amount of about 6% to 7% and the malt extracts in the amount of about 8% to 10% of the health food.

3. The composition as claimed in claim 1, in which the malt extracts are obtained by inoculating with Aspergillus oryzae in the agent for malt such as the rice bran.

4.The composition claimed in any of claim 1 to 2, which has a tablet or a solution.

5. Use of composition claimed in any of claims 1 to 3, in which the composition is used as the health food having anti-oxidant, hematopoiesis-enhancing, autonerve-modulating and immuno-enhancing properties

6. The said health food as claimed in claim 5, which has anti-oxidant activity.

7. The said health food as claimed in claim 5, which has the activity of hematopoiesis- enhancment.

8. The said health food as claimed in claim 5, which has the effect on the relaxation of sympathetic nerve system and/or the sthenia of parasympathetic nerve system.

9.The said health food as claimed in claim 5, which has the immune defence function against radiation.