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WO2008143199A1 - 新規ポリペプチド，アフィニティークロマトグラフィー用材，及びイムノグロブリンの分離及び／又は精製方法 - Google Patents

新規ポリペプチド，アフィニティークロマトグラフィー用材，及びイムノグロブリンの分離及び／又は精製方法 Download PDF

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Publication number
WO2008143199A1
WO2008143199A1 PCT/JP2008/059091 JP2008059091W WO2008143199A1 WO 2008143199 A1 WO2008143199 A1 WO 2008143199A1 JP 2008059091 W JP2008059091 W JP 2008059091W WO 2008143199 A1 WO2008143199 A1 WO 2008143199A1
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WO
WIPO (PCT)
Prior art keywords
immunoglobulin
polypeptide
affinity chromatography
purification
separation
Prior art date
Application number
PCT/JP2008/059091
Other languages
English (en)
French (fr)
Inventor
Satoshi Sato
Original Assignee
Nomadic Bioscience Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nomadic Bioscience Co., Ltd. filed Critical Nomadic Bioscience Co., Ltd.
Priority to JP2009515220A priority Critical patent/JP5522723B2/ja
Priority to US12/600,828 priority patent/US8198409B2/en
Publication of WO2008143199A1 publication Critical patent/WO2008143199A1/ja
Priority to US13/431,428 priority patent/US8357778B2/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

【課題】精製の対象たるイムノグロブリンの立体構造の安定性が確保され、かつ損傷、機能の喪失、又は意図しない機能などの不具合が引き起こされないような条件下（pH5～9，60°C未満）で、それ自身の天然の立体構造の喪失と形成のコントロールが可能な、新規イムノグロブリン結合用ポリペプチドを開発し、これをアフィニティークロマトグラフィー用支持体に固定するリガンドとして使用すること。 【解決手段】配列番号１又は配列番号２のポリペプチドの変異体であって、pH5～9，60°C未満の条件下、イムノグロブリンとの結合性が、温度によって変化し得るものであることを特徴とするポリペプチド，及びその利用。
PCT/JP2008/059091 2007-05-21 2008-05-18 新規ポリペプチド，アフィニティークロマトグラフィー用材，及びイムノグロブリンの分離及び／又は精製方法 WO2008143199A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2009515220A JP5522723B2 (ja) 2007-05-21 2008-05-18 新規ポリペプチド，アフィニティークロマトグラフィー用材，及びイムノグロブリンの分離及び／又は精製方法
US12/600,828 US8198409B2 (en) 2007-05-21 2008-05-18 Polypeptide, an affinity chromatography material, and a method for separating and/or purifying immunoglobulin
US13/431,428 US8357778B2 (en) 2007-05-21 2012-03-27 Polypeptide, an affinity chromatography material, and a method for separating and/or purifying immunoglobulin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-133778 2007-05-21
JP2007133778 2007-05-21

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/600,828 A-371-Of-International US8198409B2 (en) 2007-05-21 2008-05-18 Polypeptide, an affinity chromatography material, and a method for separating and/or purifying immunoglobulin
US13/431,428 Division US8357778B2 (en) 2007-05-21 2012-03-27 Polypeptide, an affinity chromatography material, and a method for separating and/or purifying immunoglobulin

Publications (1)

Publication Number Publication Date
WO2008143199A1 true WO2008143199A1 (ja) 2008-11-27

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Country Status (3)

Country Link
US (2) US8198409B2 (ja)
JP (1) JP5522723B2 (ja)
WO (1) WO2008143199A1 (ja)

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JP2011041475A (ja) * 2009-08-19 2011-03-03 Asahi Kasei Medical Co Ltd 抗体製造方法
WO2012086838A1 (ja) * 2010-12-24 2012-06-28 旭化成メディカル株式会社 温度応答性リガンド固定化膜モジュールを用いた生理活性物質の分離方法
WO2012086837A1 (ja) * 2010-12-24 2012-06-28 旭化成メディカル株式会社 温度応答性プロテインａの固定化方法
WO2012121409A1 (ja) * 2011-03-10 2012-09-13 旭化成メディカル株式会社 温度応答性クロマトグラフィー担体による生理活性物質の精製方法
WO2014003142A1 (ja) * 2012-06-27 2014-01-03 旭化成メディカル株式会社 抗体
WO2014003137A1 (ja) * 2012-06-27 2014-01-03 旭化成メディカル株式会社 高アフィニティー抗体、及びその製造方法
WO2014003176A1 (ja) * 2012-06-29 2014-01-03 旭化成メディカル株式会社 プロテインａのｂドメイン変異体を含むポリペプチドが結合された担体からなる吸着材
WO2014034644A1 (ja) 2012-08-27 2014-03-06 旭化成メディカル株式会社 温度応答性クロマトグラフィーによる抗体の精製方法
WO2014171437A1 (ja) 2013-04-16 2014-10-23 旭化成メディカル株式会社 抗体タンパク質の精製方法
CN104125852A (zh) * 2012-02-15 2014-10-29 弗·哈夫曼-拉罗切有限公司 基于Fc-受体的亲和色谱
WO2016152946A1 (ja) * 2015-03-26 2016-09-29 Jsr株式会社 イムノグロブリン結合タンパク質およびそれを用いたアフィニティー担体
WO2017014261A1 (ja) * 2015-07-22 2017-01-26 株式会社カネカ 抗体様タンパク質の精製方法
WO2018018011A3 (en) * 2016-07-22 2018-03-01 Amgen Inc. Methods of purifying fc-containing proteins
WO2018037742A1 (ja) 2016-08-23 2018-03-01 日立化成株式会社 吸着材
CN107849095A (zh) * 2015-07-22 2018-03-27 株式会社钟化 酸性pH范围内的抗体结合能力降低的抗体结合性蛋白质
RU2701695C2 (ru) * 2014-11-17 2019-09-30 ДжиИ Хелткер Биопроцесс АрЭндДи АБ Мутантные иммуноглобулин-связывающие полипептиды
US10683368B2 (en) 2014-11-06 2020-06-16 Hoffmann-La Roche Inc. Fc-region variants with modified FcRn-binding and methods of use

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US9238810B2 (en) * 2008-06-05 2016-01-19 Affibody Ab Polypeptide
US8592555B2 (en) * 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
SG162687A1 (en) 2008-12-24 2010-07-29 Millipore Corp Caustic stable chromatography ligands
WO2012044831A1 (en) 2010-09-30 2012-04-05 Board Of Trustees Of Northern Illinois University Library-based methods and compositions for introducing molecular switch functionality into protein affinity reagents
SG10201604554WA (en) 2011-06-08 2016-07-28 Emd Millipore Corp Chromatography matrices including novel staphylococcus aureus protein a based ligands
US10472389B2 (en) * 2013-03-14 2019-11-12 Emd Millipore Corporation Methods of increasing protein purity using protein A based chromatography
CN104059133B (zh) 2013-03-18 2019-03-15 南京金斯瑞生物科技有限公司 一类突变的具有高耐碱特性的蛋白a及其应用
WO2015103545A1 (en) * 2014-01-03 2015-07-09 Bio-Rad Laboratories, Inc. Removal of impurities from protein a eluates
CN116236819B (zh) * 2023-05-09 2023-08-04 成都佩德生物医药有限公司 一种批量纯化多肽毒素的方法及复合型双层层析柱
WO2025059162A1 (en) 2023-09-11 2025-03-20 Dana-Farber Cancer Institute, Inc. Car-engager containing il-2 variants to enhance the functionality of car t cells

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Cited By (38)

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JP2011041475A (ja) * 2009-08-19 2011-03-03 Asahi Kasei Medical Co Ltd 抗体製造方法
JPWO2012086837A1 (ja) * 2010-12-24 2014-06-05 旭化成メディカル株式会社 温度応答性プロテインａの固定化方法
WO2012086838A1 (ja) * 2010-12-24 2012-06-28 旭化成メディカル株式会社 温度応答性リガンド固定化膜モジュールを用いた生理活性物質の分離方法
WO2012086837A1 (ja) * 2010-12-24 2012-06-28 旭化成メディカル株式会社 温度応答性プロテインａの固定化方法
WO2012121409A1 (ja) * 2011-03-10 2012-09-13 旭化成メディカル株式会社 温度応答性クロマトグラフィー担体による生理活性物質の精製方法
CN104125852B (zh) * 2012-02-15 2017-03-22 弗·哈夫曼-拉罗切有限公司 基于Fc‑受体的亲和色谱
CN104125852B9 (zh) * 2012-02-15 2017-05-17 弗·哈夫曼-拉罗切有限公司 基于Fc‑受体的亲和色谱
CN104125852A (zh) * 2012-02-15 2014-10-29 弗·哈夫曼-拉罗切有限公司 基于Fc-受体的亲和色谱
WO2014003142A1 (ja) * 2012-06-27 2014-01-03 旭化成メディカル株式会社 抗体
JPWO2014003137A1 (ja) * 2012-06-27 2016-06-02 旭化成メディカル株式会社 高アフィニティー抗体、及びその製造方法
WO2014003137A1 (ja) * 2012-06-27 2014-01-03 旭化成メディカル株式会社 高アフィニティー抗体、及びその製造方法
JPWO2014003176A1 (ja) * 2012-06-29 2016-06-02 旭化成メディカル株式会社 プロテインａのｂドメイン変異体を含むポリペプチドが結合された担体からなる吸着材
WO2014003176A1 (ja) * 2012-06-29 2014-01-03 旭化成メディカル株式会社 プロテインａのｂドメイン変異体を含むポリペプチドが結合された担体からなる吸着材
WO2014034644A1 (ja) 2012-08-27 2014-03-06 旭化成メディカル株式会社 温度応答性クロマトグラフィーによる抗体の精製方法
JPWO2014034644A1 (ja) * 2012-08-27 2016-08-08 旭化成メディカル株式会社 温度応答性クロマトグラフィーによる抗体の精製方法
WO2014171437A1 (ja) 2013-04-16 2014-10-23 旭化成メディカル株式会社 抗体タンパク質の精製方法
US10683368B2 (en) 2014-11-06 2020-06-16 Hoffmann-La Roche Inc. Fc-region variants with modified FcRn-binding and methods of use
US11440971B2 (en) 2014-11-06 2022-09-13 Hoffmann-La Roche Inc. Fc-region variants with modified FcRn-binding and methods of use
RU2701695C2 (ru) * 2014-11-17 2019-09-30 ДжиИ Хелткер Биопроцесс АрЭндДи АБ Мутантные иммуноглобулин-связывающие полипептиды
JPWO2016152946A1 (ja) * 2015-03-26 2018-01-18 Jsr株式会社 イムノグロブリン結合タンパク質およびそれを用いたアフィニティー担体
US10723769B2 (en) 2015-03-26 2020-07-28 Jsr Corporation Immunoglobulin-binding protein and affinity carrier using same
WO2016152946A1 (ja) * 2015-03-26 2016-09-29 Jsr株式会社 イムノグロブリン結合タンパク質およびそれを用いたアフィニティー担体
CN107849095A (zh) * 2015-07-22 2018-03-27 株式会社钟化 酸性pH范围内的抗体结合能力降低的抗体结合性蛋白质
CN107849088A (zh) * 2015-07-22 2018-03-27 株式会社钟化 抗体样蛋白质的纯化方法
JPWO2017014261A1 (ja) * 2015-07-22 2018-04-26 株式会社カネカ 抗体様タンパク質の精製方法
WO2017014261A1 (ja) * 2015-07-22 2017-01-26 株式会社カネカ 抗体様タンパク質の精製方法
KR20220120716A (ko) * 2016-07-22 2022-08-30 암젠 인크 Fc-함유 단백질의 정제 방법
JP2019528255A (ja) * 2016-07-22 2019-10-10 アムジエン・インコーポレーテツド Ｆｃ含有タンパク質を精製する方法
JP7092744B2 (ja) 2016-07-22 2022-06-28 アムジエン・インコーポレーテツド Ｆｃ含有タンパク質を精製する方法
KR102435801B1 (ko) * 2016-07-22 2022-08-25 암젠 인크 Fc-함유 단백질의 정제 방법
KR20190037247A (ko) * 2016-07-22 2019-04-05 암젠 인크 Fc-함유 단백질의 정제 방법
IL285146B (en) * 2016-07-22 2022-09-01 Amgen Inc Methods of purifying fc-containing proteins
WO2018018011A3 (en) * 2016-07-22 2018-03-01 Amgen Inc. Methods of purifying fc-containing proteins
CN115925780A (zh) * 2016-07-22 2023-04-07 美国安进公司 含有Fc的蛋白的纯化方法
AU2017298984B2 (en) * 2016-07-22 2023-08-31 Amgen Inc. Methods of purifying Fc-containing proteins
KR102579850B1 (ko) * 2016-07-22 2023-09-18 암젠 인크 Fc-함유 단백질의 정제 방법
WO2018037742A1 (ja) 2016-08-23 2018-03-01 日立化成株式会社 吸着材
US10898878B2 (en) 2016-08-23 2021-01-26 Showa Denko Materials Co., Ltd. Adsorbent material

Also Published As

Publication number Publication date
JP5522723B2 (ja) 2014-06-18
US8357778B2 (en) 2013-01-22
JPWO2008143199A1 (ja) 2010-08-05
US8198409B2 (en) 2012-06-12
US20120184711A1 (en) 2012-07-19
US20100168395A1 (en) 2010-07-01

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