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WO2008038424A1 - Vaisseau sanguin artificiel pour petite artère utilisant des fils de fibroïne - Google Patents

Vaisseau sanguin artificiel pour petite artère utilisant des fils de fibroïne Download PDF

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Publication number
WO2008038424A1
WO2008038424A1 PCT/JP2007/055818 JP2007055818W WO2008038424A1 WO 2008038424 A1 WO2008038424 A1 WO 2008038424A1 JP 2007055818 W JP2007055818 W JP 2007055818W WO 2008038424 A1 WO2008038424 A1 WO 2008038424A1
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Prior art keywords
blood vessel
artificial blood
small
diameter
mouth
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English (en)
Japanese (ja)
Inventor
Masataka Sata
Kazuo Hatae
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Japan Science and Technology Agency
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Japan Science and Technology Agency
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/28—Materials for coating prostheses
    • A61L27/34—Macromolecular materials

Definitions

  • the present invention relates to a small-diameter artificial blood vessel using a hive-in yarn and a method for producing the small-diameter artificial blood vessel.
  • the collection of own blood vessels is not only a burden on the patient, but the own blood vessels may not be used for revascularization.
  • the own blood vessels may not be used for revascularization.
  • the left and right internal thoracic arteries, gastric arterial artery, and radial artery used for coronary artery bypass surgery can only be acquired once in the lifetime of the patient. Even with these points, there is a great expectation for an artificial blood vessel that can be supplied without relying on its own blood vessel, and the development of a small-diameter artificial blood vessel with good patency has been desired.
  • a bioabsorbable artificial blood vessel can be clinically applied in a low-pressure system such as a pulmonary artery or a vein.
  • a high-pressure system such as a systemic circulatory artery is bioabsorbable.
  • durability There is a problem of durability. For this reason, the period until absorption in a living body with high strength is moderately long, and there is no problem with durability, that is, it has moderate bioabsorbability and can be used in high-pressure systems such as arterial circulatory arteries. Development of small-diameter artificial blood vessels has been desired.
  • Patent Document 1 describes a kite structure formed by winding a kite string around a mandrel according to the principle of braid making and a manufacturing method thereof.
  • this kite structure it is described as a preferred mode that the kite is glued with sericin to secure strength, and further, synthetic fiber or metal fiber is mixed.
  • this silk thread structure is a structure pursuing strength, and synthetic fibers and metal fibers used by mixing are problematic in terms of bioabsorbability when used as small-diameter artificial blood vessels. Arise.
  • the patency of this silk thread structure has not been studied, and it cannot be expected that it can be used as a small-diameter artificial blood vessel.
  • Patent Document 1 Japanese Patent Application Laid-Open No. 2004-173722
  • the present invention has good patency, a period of time until absorption in a living body with high strength is appropriately long, and does not cause a problem in durability, that is, has an appropriate bioabsorbability, It is an object of the present invention to provide a small-diameter artificial blood vessel that can be used in a high-pressure system such as a circulatory artery and a method for producing the small-diameter artificial blood vessel.
  • the inventors of the present invention have a small-diameter artificial device that has good patency when used as a small-diameter artificial blood vessel and has strength that can be used even in a high-pressure system and appropriate bioabsorbability.
  • a small-diameter artificial blood vessel is actually created and transplanted into an experimental animal and observed over time.
  • a method for producing a small-diameter artificial blood vessel comprising a multi-layered cylindrical structure formed by braiding a multi-layered brave-in yarn produced by the production method. Found to be achieved by small-diameter artificial blood vessels.
  • the small-diameter artificial blood vessel of the present invention was confirmed to have a long-term patency of 4 weeks to 32 weeks or more by anastomosing the rat abdominal aorta as a small-diameter artificial blood vessel with a diameter of 1.5 mm. It was.
  • the present invention is the following [1] to [: 17].
  • [7] A warp yarn used as a hive-in yarn, and a multilayered cylindrical structure formed. The sericin is removed and then coated with a hive-in. Any one of [1] to [6] A small-diameter artificial blood vessel described in 1.
  • a method for producing a small-diameter artificial blood vessel comprising:
  • the step of coating the multi-layered cylindrical structure with the fiber mouth-in includes:
  • a process for forming a multilayered tubular structure by braiding woven yarn-in yarns in multiple layers, and using a kite yarn as a woven yarn-in yarn. After the step,
  • the present invention also includes the following [18] to [21].
  • An angiogenesis inducer comprising the small-diameter artificial blood vessel according to any one of [1] to [7] and [17].
  • the small-diameter artificial blood vessel of the present invention is a small-diameter artificial blood vessel having a diameter of 5. Omm or less, and enables long-term patency without causing thrombotic occlusion.
  • This long-term patency is considered to be brought about by the antithrombogenicity of the small-diameter artificial blood vessel of the present invention and vascularization by migration of endothelial cells and smooth muscle cells into the small-diameter artificial blood vessel lumen. Therefore, it can be expected to maintain a stable patency for a long time.
  • the small-diameter artificial blood vessel of the present invention If used, revascularization can be performed without relying on the own blood vessel, and the small-diameter artificial blood vessel of the present invention cannot already collect the own blood vessel. It greatly contributes to the lifesaving of patients who have become.
  • the small-diameter artificial blood vessel of the present invention has an appropriate bioabsorbability, it can be suitably used in revascularization for pediatric patients in which remodeling due to growth is a problem. It is possible.
  • the small-diameter artificial blood vessel of the present invention has sufficient strength and durability due to moderate bioabsorbability, it is not suitable for conventional bioabsorbable materials. It can be suitably used.
  • FIG. 1 is a view showing a three-layered small-diameter artificial blood vessel using a five mouth-in and an appearance immediately after implantation into a rat abdominal aorta.
  • FIG. 2 is a view showing an image obtained by observing the luminal surface of a three-layered small-diameter artificial blood vessel with a implanted mouth-in with a scanning electron microscope.
  • FIG. 3 is a diagram showing the appearance, ultrasonic diagnostic image, and angiogram of a small-diameter artificial blood vessel having a three-layer structure using a transplanted mouth opening.
  • FIG. 4 is a view showing a histological observation image of a cross-section of a three-layered small-diameter artificial blood vessel using a transplanted mouth opening.
  • Fig. 5 is a view showing an appearance of a cross section of a three-layered small-diameter artificial blood vessel by a transplanted mouth-in and a histological observation image.
  • Fig. 6 shows a cross-sectional tissue of a three-layered small-diameter artificial blood vessel using a transplanted mouth-in. It is a figure which shows a scientific observation image.
  • Fig. 7 is a diagram showing the appearance, cross-sectional appearance, and histological observation image of a three-layered small-diameter artificial blood vessel with a transplanted mouth opening.
  • Fig. 8 is a diagram showing a polarization microscope image of a cross section of a three-layered small-diameter artificial blood vessel using a transplanted mouth-in.
  • FIG. 9 is a view showing a histological observation image of a cross section of a three-layered small-diameter artificial blood vessel using a transplanted mouth opening.
  • FIG. 10 is a view showing a three-layered small-diameter artificial blood vessel using a fiber mouth-in and a small-diameter artificial blood vessel of PTFE and an appearance immediately after implantation into a rat abdominal aorta.
  • Fig. 11 is a diagram showing an ultrasonic diagnostic image of a three-layered small-diameter artificial blood vessel and a PTFE-made small-diameter artificial blood vessel using a transplanted mouth-in.
  • FIG. 12 is a diagram showing angiograms of a three-layered small-diameter artificial blood vessel and a PTFE-made small-diameter artificial blood vessel using a transplanted mouth-in.
  • FIG. 13 is a graph showing the quantification of the patency of a three-layered small-diameter artificial blood vessel using PT and a small-diameter artificial blood vessel made of PTFE.
  • FIG. 14 is a diagram showing a histological observation image of a cross section of a transplanted PTFE small-diameter artificial blood vessel.
  • FIG. 15 is a view showing a single-layered small-diameter artificial blood vessel by PT and a small-diameter artificial blood vessel made of PTFE and the appearance immediately after implantation into the abdominal aorta of a rat.
  • FIG. 16 is a view showing a histological observation image of a cross section of a small-diameter artificial blood vessel having a single-layer structure and a small-diameter artificial blood vessel made of PTFE by a fiber mouth-in.
  • FIG. 17 is a view showing an image obtained by observing the inner surface of a small-diameter artificial blood vessel having a one-layer structure and a small-diameter artificial blood vessel made of PTFE by a scanning electron microscope using a transplanted mouth-in.
  • FIG. 18 is a diagram showing the cross-sectional appearance and histological observation images of a one-layered small-diameter artificial blood vessel and a PTFE-made small-diameter artificial blood vessel using a transplanted mouth opening.
  • FIG. 19 is a diagram showing an image obtained by observing the inner surface of a small-diameter artificial blood vessel having a one-layer structure and a small-diameter artificial blood vessel made of PTFE with a scanning electron microscope using an implanted fiber mouth-in.
  • FIG. 20 is a graph showing a quantification of the patency of a small-diameter artificial blood vessel having a single-layer structure and a PTFE-made small-diameter artificial blood vessel by a fiber mouth-in.
  • the small-diameter artificial blood vessel according to the present invention is a small-diameter artificial blood vessel in which a multi-layered cylindrical structure formed by knitting a braided-in yarn in multiple layers is covered with a fibre-in.
  • This small-diameter artificial blood vessel is produced by the following manufacturing method according to the present invention:
  • It can be manufactured by a method for manufacturing a small-diameter artificial blood vessel.
  • the multilayer described above is preferably three or four layers, particularly preferably three layers. If this is a single layer without multiple layers, it will not be possible to obtain strength that can be used in high pressure systems. If there are many layers, the strength that is improved in terms of strength will be inferior in the flexibility required for an artificial blood vessel, and the period of bioabsorption will be too long.
  • the small-diameter artificial blood vessel of the present invention has long-term patency.
  • long-term patency means that the artificial blood vessel remains open without being blocked for a sufficient period of time (for example, several weeks or more for rats and several months or more for humans) after transplantation of the artificial blood vessels. Refers to nature.
  • the step of coating the multilayered cylindrical structure with the fiber mouth-in can be carried out by immersing the multilayered cylindrical structure in the fiber mouth-in solution.
  • the hive mouth in solution is preferably a solution in which the hive mouth in is dissolved in an aqueous lithium bromide solution, and more preferably a dialysis after the hive mouth in is dissolved in an aqueous lithium bromide solution.
  • This hive mouth-in solution can generally be used with a hive mouth-in concentration in the range of 0.5 to 8%, preferably in the range of 1 to 4%, particularly preferably in the range of! To 3%.
  • One with a mouth-in concentration of 5% can be used.
  • the immersion time is generally a force that can be appropriately selected from the range of 1 to 120 seconds, preferably 1 to 60 seconds, and particularly preferably 1 to 30 seconds.
  • the excellent characteristic of the small-diameter artificial blood vessel of the present invention is that a multilayered cylindrical structure formed by knitting a brave-in yarn in multiple layers is covered with the fibre-in. It is thought that This multi-layered structure, particularly preferably a three-layered cylindrical structure, is coated with the fibre-in, so that the ability to induce migration and colonization of vascular endothelial cells and Z or smooth muscle cells shown in the present invention can be achieved.
  • the bioabsorbability is maintained to be moderate, and that the artificial blood vessel is changed so as to be replaced with the same blood vessel as the original blood vessel (vascularization).
  • the three-layered cylindrical structure is appropriately fixed by the mesh-in coating, the entire shape is properly maintained, and the end of the cylindrical structure is not frayed. At the same time, it is considered that the plant has been stabilized even after transplanting.
  • these main effects of stabilizing the shape are the preferred embodiments of the present invention, in which a warp yarn is used as the hive-in yarn, and the formed multi-layered cylindrical structure has sericin removed. If it is, it is particularly effective.
  • the inner diameter of the small-diameter artificial blood vessel is generally in the range of 1.0 to 5. Omm, preferably in the range of 1.0 to 4.0 mm, more preferably in the range of 1.0 to 3 ⁇ Omm, particularly preferably. May be in the range of 1.0 to 2. Omm.
  • the small-diameter artificial blood vessel of the present invention has an advantageous feature that it has sufficient patency even when it has such a small inner diameter.
  • This inner diameter can be set to a desired inner diameter in the surgical procedure, and a force that can be set to an inner diameter smaller than this range. The smaller the inner diameter is, the more difficult the end-to-end anastomosis is.
  • the contribution of the present invention is that an artificial blood vessel having a small diameter that cannot be realized as a practical one is provided.
  • Omm can be done.
  • the diameter of this mandrel generally ranges from 1.0 to 5.
  • Omm depending on the desired inner diameter
  • it is in the range of 1 ⁇ 0 to 4 ⁇ Omm, more preferably in the range of 1.0 to 3.
  • Braid knitting can be performed with a known method and apparatus.
  • a particularly advantageous effect is achieved by the combination of the above-mentioned multilayer structure, preferably three or four layers, particularly preferably three layers, and coating with a fiber mouth-in.
  • initial blood leakage that cannot be sufficiently prevented by simply forming a multilayer structure is prevented by the coating of the fibre-in, and further, the gap between the layers of the multilayer structure is prevented by the adhesive effect of this fiproin coating.
  • anti-thrombogenicity is conferred by the five mouth in.
  • the small-diameter artificial blood vessel of the present invention has a very effective ability to vascularize an artificial blood vessel.
  • This vascularization ability is exerted over the entire cross section of the artificial blood vessel, but is particularly favorably exhibited in the lumen of the artificial blood vessel.
  • This ability to induce vascularization that is, the ability to induce the artificial blood vessel to change in the same way as the original self blood vessel, is induced by the ability of the small-diameter artificial blood vessel of the present invention to induce the migration of endothelial cells and / or smooth muscle cells. It is thought that it is. Further, this point force can be used as an agent for inducing migration of endothelial cells and / or smooth muscle cells, and can also be used as an agent for inducing angiogenesis.
  • the small-diameter artificial blood vessel of the present invention can be used in arteries of the systemic circulation system, that is, has sufficient strength to enable this.
  • the sericin is used as the fiber-in yarn and the sericin is removed from the multi-layered cylindrical structure formed, It is coated with in.
  • Use of a yarn yarn in this way is advantageous in that it can easily form a cylindrical structure having a certain strength.
  • This silk yarn contains sericin in addition to the fiber mouth-in. By removing this sericin, the possibility of causing an allergic reaction as a heterologous antigen is greatly reduced.
  • the process of forming a multilayered tubular structure by braiding the brave-in yarn in multiple layers is performed using a kite yarn as the fibre-in yarn, and after the step, After removing the sericin from the formed multi-layered cylindrical structure, It can be manufactured by a manufacturing method that performs a step of coating a cylindrical structure with a fibre-in.
  • the sericin can be removed by a known method used in the scouring of silk thread, for example, by immersing in a heated alkaline aqueous solution and washing with water.
  • alkaline aqueous solution examples include a sodium carbonate aqueous solution and a sodium hydrogen carbonate aqueous solution.
  • the heating temperature is generally in the range of 90 ° C. or higher, preferably 98 ° C. or higher, and this can be preferably carried out by boiling at atmospheric pressure.
  • the washing with water can be performed at room temperature, for example. Thorough washing with water is preferable.
  • the water used for washing is preferably high-purity water such as distilled water, deionized water, or filter permeated water.
  • a so-called PTFE commercially available for animal clinical experiments that is, ePTFE (expanded poiytetrailuoroethylene: gore-tex) / J, caliber artificial blood vessel (manufactured by Nyanon Gotex Co., Ltd., trade name: Gortex (registered trademark) artificial blood vessel) was used.
  • ePTFE expanded poiytetrailuoroethylene: gore-tex
  • caliber artificial blood vessel manufactured by Nyanon Gotex Co., Ltd., trade name: Gortex (registered trademark) artificial blood vessel
  • a three-layered small-diameter artificial blood vessel made of fibroin and a small-diameter artificial blood vessel made of PTFE were implanted into the abdominal aorta of a rat (Sprague Dawley Rat, 10-14 weeks old, male) to observe its progress. It was.
  • Transplantation was performed by end-to-end anastomosis of a small-diameter artificial blood vessel to the abdominal aorta.
  • the patency of the lumen of the artificial blood vessel was confirmed by an ultrasonic diagnostic apparatus, and a histological examination of the cross section of the artificial blood vessel was also performed.
  • the following shows the experiment and results of transplanting a three-layered small-diameter artificial blood vessel with a fiber mouth-in.
  • FIG. 1 shows a three-layered small-diameter artificial blood vessel (top of Fig. 1) (with a diameter of 2. Omm and a length of 15 mm) with a five mouth-in used for transplantation into rats, and the rat abdominal aorta The appearance immediately after transplantation is shown (bottom of Fig. 1). End-to-end anastomosis of this caliber was a very difficult operation, and it was possible to stably implant force artificial blood vessels. The end of the artificial blood vessel is stable and excessive from the artificial blood vessel There was no bleeding.
  • Figure 2 shows the surface of the luminal surface of a three-layered small-diameter artificial blood vessel with a implanted mouth-in by means of a scanning electron microscope at 2 weeks (2w) and 4 weeks (4w) after transplantation. The image is shown. Cells thought to be vascular endothelial cells have already migrated and settled after 2 weeks, and after 4 weeks almost completely covered the inner surface of the three-layered small-diameter artificial blood vessel with a five mouth-in. It was observed. In other words, a three-layered small-diameter artificial blood vessel with a implanted fiber mouth-in has the ability to induce migration and colonization of these cells, and has the same luminal surface as the original blood vessel. I found it.
  • Fig. 3 shows the appearance, ultrasonic diagnostic image, and angiogram of a three-layered small-diameter artificial blood vessel with a five mouth-in 12 weeks after transplantation.
  • the three-layered small-diameter artificial blood vessel with the implanted fiber mouth-in was found to be extremely stable even 12 weeks after transplantation, and functioned like the original aorta.
  • Fig. 4 shows histological observation images by HE staining of a cross section of a three-layered small-diameter artificial blood vessel with a five mouth in 2 weeks, 4 weeks, and 12 weeks after transplantation.
  • Fig. 5 shows the appearance and cross-sectional view of a three-layered small-diameter artificial blood vessel with a hive mouth-in 4 weeks after transplantation (HE, CD31, SMA) and transmission electron microscope image ( T EM).
  • HE high-density polyethylene
  • CD31 high-density polyethylene
  • SMA transmission electron microscope image
  • EC endothelial cells
  • SMC smooth muscle cells
  • the three-layered small-diameter artificial blood vessel with a fiber mouth-in was found to be highly biocompatible.
  • Fig. 6 shows a histological observation image (HE, CD31, CD68, SMA) of a cross-section of a three-layered small-diameter artificial blood vessel with a five mouth-in 12 weeks after transplantation.
  • CD68 stains monocytes around fibroin
  • SMA stains smooth muscle cells
  • CD31 stains endothelial cells. It was found that the three-layered small-diameter artificial blood vessel with a fiber mouth-in formed a structure very similar to the original blood vessel wall with high biocompatibility.
  • FIG. 7 shows the appearance of a three-layered small-diameter artificial blood vessel with a five mouth-in 24 weeks after transplantation, the appearance of a cross section, and a histological observation image by HE staining.
  • a three-layered small-diameter artificial blood vessel with a implanted mouth-in is similar to the original aorta 24 weeks after transplantation. It has an external appearance and a cross-section, has patency, and functions in the same manner as the original aorta.
  • the small-diameter artificial blood vessel with a three-layer structure formed by the transplanted fiber mouth-in has a very effective ability to induce angiogenesis (angiogenic ability). And I knew that.
  • FIG. 8 shows polarized microscopic images of Sirius red stained sections of a three-layered small-diameter artificial blood vessel with a fiber mouth-in 2 weeks and 24 weeks after transplantation.
  • Collagen fiber strength which was not observed much after 2 weeks, it was observed that many fibers were formed along the fiber mouth-in fibers after 24 weeks.
  • the ability to induce vascularization (vascularization ability) of a three-layered small-diameter artificial blood vessel by phive in extends not only to cell migration and colonization, but also to the reconstruction of the vascular structure formed by the extracellular matrix. It was a power to be in trouble.
  • the ability to form such collagen fibers contributes to the provision of sufficient strength and durability over a long period of time, which is an advantageous feature of the three-layered small-diameter artificial blood vessel using the fiber mouth-in of the present invention. it is conceivable that.
  • FIG. 9 shows a histological observation image (CD31, a SMA, Masson, Siriusred) of a cross-section of a three-layered small-diameter artificial blood vessel with a five mouth-in 24 weeks after transplantation. It was found that the structure similar to the blood vessel wall becomes closer to the blood vessel wall, and the collagen fibers contributing to the strength are becoming denser.
  • Fig. 10 shows a three-layered small-diameter artificial blood vessel (upper left in Fig. 10) (caliber: 1.5 mm, length: 10 mm) and the rat abdominal aorta.
  • the rat Fig. 10 shows the appearance immediately after transplantation into the abdominal aorta.
  • FIG. 11 shows an ultrasonic diagnostic image of a three-layered small-diameter artificial blood vessel and PTFE-made small-diameter artificial blood vessel, which are produced by a five mouth-in two weeks after transplantation. It was found that the three-layered small-diameter artificial blood vessel with a fiber mouth-in showed good patency, while the small-diameter artificial blood vessel made of PTFE did not show good patency.
  • FIG. 12 shows angiograms of a three-layered small-diameter artificial blood vessel and a PTFE-made small-diameter artificial blood vessel with a five mouth-in 12 weeks after transplantation.
  • a three-layered small-diameter artificial blood vessel with a fiber mouth-in showed good patency, while a small-diameter artificial blood vessel made of PTFE was found to be inferior in patency.
  • Fig. 13 shows a comparison of the patency of a three-layered small-diameter artificial blood vessel with a fiber mouth-in and a small-diameter human blood vessel made of PTFE by echocardiography, showing an individual with sufficient patency.
  • a graph quantifying the percentage of numbers as the patency rate is shown.
  • the patency rate of the three-layered small-diameter vascular prosthesis by the fiber mouth-in was 95%, compared with the patency rate of 35% for PTFE-made small-diameter artificial blood vessels.
  • the patency rate is more than 3 times.
  • the 95% patency rate of the three-layered small-diameter artificial blood vessel by this fiber mouth is reduced, except for individuals whose patency problem occurred at the very early stage, even after 32 weeks after transplantation. The results are expected to maintain excellent patency for the lifetime.
  • FIG. 14 shows histological observation images of cross sections of PTFE small-diameter artificial blood vessels at 2 weeks (2w) and 4 weeks (4w) after transplantation.
  • the low patency of PTFE small-diameter artificial blood vessels as shown in Fig. 13 has been shown to be due to thrombus formation.
  • Fig. 15 shows a one-layered small-diameter artificial blood vessel with a fiber mouth-in used for transplantation to a rabbit.
  • FIG. 16 shows a histological observation image (HE, CD31) of a cross section of a small-diameter artificial blood vessel having a single-layer structure and PTFE-made small-diameter artificial blood vessel by a five mouth in 4 weeks after transplantation.
  • HE histological observation image
  • FIG. 17 shows images obtained by observing the luminal surface of a single-layered small-diameter artificial blood vessel and PTFE-made small-diameter artificial blood vessel with a fiber mouth-in 4 weeks after transplantation using a scanning electron microscope. Only a few vascular endothelial cell migrations and colonies were observed.
  • FIG. 18 shows the cross-sectional appearance and histological observation image of the single-layered small-diameter artificial blood vessel and PTFE-made small-diameter artificial blood vessel that were produced three months after transplantation by the five mouth-in. In both cases, cell colonization and accompanying vascularization were insufficient.
  • FIG. 19 shows images obtained by observing the inner surface of a small-diameter artificial blood vessel having a single layer structure and a small-diameter artificial blood vessel made of PTFE with a five mouth three months after transplantation using a scanning electron microscope. Even at this time, vascular endothelial cells and the like were not sufficiently established.
  • Figure 20 shows the number of individuals showing sufficient patency by comparing the patency of a one-layered small-diameter artificial blood vessel with a fiber mouth-in and a small-diameter human blood vessel made of PTFE by echocardiography.
  • the graph which quantified the ratio of as a patency rate is shown.
  • the one-layered small-diameter artificial blood vessel with a fiber mouth-in and the small-diameter artificial blood vessel made of PTFE are less than 70% after 12 weeks. The rate of patency was low and low.

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Abstract

La présente invention concerne un vaisseau sanguin artificiel de petit diamètre qui présente une perméabilité souhaitable ainsi qu'une grande résistance, fait preuve d'une performance de bioabsorption appropriée, et s'avère susceptible d'être utilisé même dans un environnement de haute pression, tel qu'une artère de la circulation systémique. L'invention concerne également un procédé permettant de produire le vaisseau sanguin artificiel de petit diamètre. Elle concerne en outre un vaisseau sanguin artificiel de petit diamètre comportant une structure tubulaire à couches multiples revêtue de fibroïne, la structure tubulaire à couches multiples résultant d'un tricotage de tresses en couches multiples de fils de fibroïne. L'invention concerne de plus un procédé de production d'un vaisseau sanguin artificiel de petit diamètre, comportant les étapes qui consistent à former une structure tubulaire à couches multiples par le tricotage de tresses en couches multiples de fils de fibroïne, ainsi que le revêtement de la structure tubulaire à couches multiples avec de la fibroïne.
PCT/JP2007/055818 2006-09-25 2007-03-22 Vaisseau sanguin artificiel pour petite artère utilisant des fils de fibroïne Ceased WO2008038424A1 (fr)

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JP2006258586A JP4541336B2 (ja) 2006-09-25 2006-09-25 フィブロイン糸を使用した小動脈用人工血管
JP2006-258586 2006-09-25

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CN102343113A (zh) * 2010-08-02 2012-02-08 苏州大学 用于组织修复的丝素蛋白管状支架的制备方法
WO2012111309A1 (fr) * 2011-02-18 2012-08-23 福井経編興業株式会社 Tube double raschel tricoté pour vaisseaux sanguins synthétiques et son procédé de production
CN104524632A (zh) * 2015-01-21 2015-04-22 北京航空航天大学 一种具有良好顺应性的抗凝血复合管状支架的制备方法
CN105457101A (zh) * 2015-12-17 2016-04-06 华南理工大学 一种三层结构小口径血管支架的制备方法
CN113952075A (zh) * 2020-10-28 2022-01-21 清华大学 一种多尺度复合结构仿生小口径人工血管及其制备方法
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