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WO2008032058A3 - Method for sequencing a polynucleotide - Google Patents

Method for sequencing a polynucleotide Download PDF

Info

Publication number
WO2008032058A3
WO2008032058A3 PCT/GB2007/003451 GB2007003451W WO2008032058A3 WO 2008032058 A3 WO2008032058 A3 WO 2008032058A3 GB 2007003451 W GB2007003451 W GB 2007003451W WO 2008032058 A3 WO2008032058 A3 WO 2008032058A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotide
concatemer
sequencing
target polynucleotide
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2007/003451
Other languages
French (fr)
Other versions
WO2008032058A2 (en
Inventor
Preben Lexow
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lingvitae AS
Original Assignee
Lingvitae AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lingvitae AS filed Critical Lingvitae AS
Publication of WO2008032058A2 publication Critical patent/WO2008032058A2/en
Publication of WO2008032058A3 publication Critical patent/WO2008032058A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is a method for sequencing a target polynucleotide, comprising the steps of: (i) ligating the target polynucleotide to a first polynucleotide; (ii) forming a concatemer comprising multiple copies of the product of step (i); (iii) attaching the concatemer to a second polynucleotide such that the second polynucleotide hybridises to portions of the concatemer, but not to regions on the concatemer corresponding to at least a portion of the target polynucleotide; (iv) interrogating one or more bases in those regions not hybridised to the second polynucleotide, to thereby identify the target polynucleotide sequence.
PCT/GB2007/003451 2006-09-13 2007-09-13 Method for sequencing a polynucleotide Ceased WO2008032058A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0618046.7 2006-09-13
GB0618046A GB0618046D0 (en) 2006-09-13 2006-09-13 Method

Publications (2)

Publication Number Publication Date
WO2008032058A2 WO2008032058A2 (en) 2008-03-20
WO2008032058A3 true WO2008032058A3 (en) 2008-10-09

Family

ID=37309875

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/003451 Ceased WO2008032058A2 (en) 2006-09-13 2007-09-13 Method for sequencing a polynucleotide

Country Status (2)

Country Link
GB (1) GB0618046D0 (en)
WO (1) WO2008032058A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
KR20150132481A (en) 2013-03-15 2015-11-25 테라노스, 인코포레이티드 Nucleic Acid Amplification
EP2970961B1 (en) 2013-03-15 2019-04-24 Theranos IP Company, LLC Nucleic acid amplification
ES2729637T3 (en) 2013-03-15 2019-11-05 Theranos Ip Co Llc Nucleic acid amplification
MX356055B (en) 2013-09-06 2018-05-11 Theranos Ip Co Llc SYSTEMS and METHODS FOR DETECTING INFECTIOUS DISEASES.
EP3957741A1 (en) * 2013-11-22 2022-02-23 Labrador Diagnostics LLC Nucleic acid amplification

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001020039A2 (en) * 1999-09-16 2001-03-22 Curagen Corporation Method of sequencing a nucleic acid
US20030215821A1 (en) * 1999-04-20 2003-11-20 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US20040110153A1 (en) * 2002-12-10 2004-06-10 Affymetrix, Inc. Compleixity management of genomic DNA by semi-specific amplification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030215821A1 (en) * 1999-04-20 2003-11-20 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
WO2001020039A2 (en) * 1999-09-16 2001-03-22 Curagen Corporation Method of sequencing a nucleic acid
US20040110153A1 (en) * 2002-12-10 2004-06-10 Affymetrix, Inc. Compleixity management of genomic DNA by semi-specific amplification

Also Published As

Publication number Publication date
WO2008032058A2 (en) 2008-03-20
GB0618046D0 (en) 2006-10-25

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