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WO2008030995A8 - Use of novel pepc to produce succinate - Google Patents

Use of novel pepc to produce succinate Download PDF

Info

Publication number
WO2008030995A8
WO2008030995A8 PCT/US2007/077806 US2007077806W WO2008030995A8 WO 2008030995 A8 WO2008030995 A8 WO 2008030995A8 US 2007077806 W US2007077806 W US 2007077806W WO 2008030995 A8 WO2008030995 A8 WO 2008030995A8
Authority
WO
WIPO (PCT)
Prior art keywords
pepc
novel
succinate
produce succinate
engineered bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/077806
Other languages
French (fr)
Other versions
WO2008030995A3 (en
WO2008030995A2 (en
Inventor
Ka-Yiu San
George N Bennett
Mary Lou Harrison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
William Marsh Rice University
Original Assignee
William Marsh Rice University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by William Marsh Rice University filed Critical William Marsh Rice University
Publication of WO2008030995A2 publication Critical patent/WO2008030995A2/en
Publication of WO2008030995A3 publication Critical patent/WO2008030995A3/en
Anticipated expiration legal-status Critical
Publication of WO2008030995A8 publication Critical patent/WO2008030995A8/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A Leuconostoc mesenteroides phosphoenolpyruvate carboxylase enzyme has been identified that improves succinate production in engineered bacteria. Expression of the novel PEPC enzyme by engineered bacteria increases production and conversion of carbon to product is improved. Methods are described for making succinate using bacterial cultures expressing L. mesenteroides PEPC.
PCT/US2007/077806 2006-09-06 2007-09-06 Use of novel pepc to produce succinate Ceased WO2008030995A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82471706P 2006-09-06 2006-09-06
US60/824,717 2006-09-06

Publications (3)

Publication Number Publication Date
WO2008030995A2 WO2008030995A2 (en) 2008-03-13
WO2008030995A3 WO2008030995A3 (en) 2008-10-23
WO2008030995A8 true WO2008030995A8 (en) 2009-07-16

Family

ID=39158070

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/077806 Ceased WO2008030995A2 (en) 2006-09-06 2007-09-06 Use of novel pepc to produce succinate

Country Status (1)

Country Link
WO (1) WO2008030995A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7569380B2 (en) 2004-12-22 2009-08-04 Rice University Simultaneous anaerobic production of isoamyl acetate and succinic acid
US8962272B2 (en) 2010-06-21 2015-02-24 William Marsh Rice University Engineered bacteria produce succinate from sucrose

Also Published As

Publication number Publication date
WO2008030995A3 (en) 2008-10-23
WO2008030995A2 (en) 2008-03-13

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