WO2008018118A1 - Composition containing nemagaritake and moisturizing agent, cellular stimulant, whitening agent and antioxidant - Google Patents

Abstract

It is intended to provide a composition which contains, as the active ingredient, nemagaritake (Sasa kurilensis (Rupr) Makino) and has a moisturizing, cell stimulating, whitening or antioxidant effect. By adding it to compositions such as a skin preparation for external use, a food or a drink, it is possible to obtain various compositions exerting excellent effects of preventing or ameliorating skin conditions such as wrinkles, skin fitness, spots, dullness and so on.

Description

 Specification

 Nemagari-dake-containing composition, moisturizer, cell activator, whitening agent and antioxidant

 [0001] The present invention relates to a nematode-containing composition, a humectant, a cell activator, a whitening agent and an antioxidant.

 Background art

 [0002] Conventionally, various moisturizers, cell activators, whitening agents and antioxidants have been used to prevent and improve skin wrinkles, rough skin, tarmi, reduced skin elasticity, stains and freckles! Has been studied. However, the development of highly effective ingredients is still awaited.

 [0003] On the other hand, naturally derived ingredients are known to have various pharmacological and cosmetic effects, and so far many plants and fungi have been widely applied in the fields of topical skin preparations and foods and drinks. Yes. However, there are many naturally-derived components whose effects are not yet known, and there is ample room for development research.

[0004] To date, regarding bamboo sasa plants, health foods and oral anti-tumor agents (Patent Document 1), anti-tumor agent compositions (Patent Document 2), anti-allergies that contain the extract as an active ingredient Agents (Patent Document 3) and antibacterial agents (Patent Document 4) are known.

[0005] As for Negararidake (Poaceae, Sara kurilensis), the extract is formulated as a keratinase inhibitor into a topical skin preparation (Patent Document 5), and into a cosmetic for hair.

(Patent Document 6) is known. In addition, there is also known a cosmetic liquid containing tishima zasa distillate obtained by fractionating a squeezed juice obtained by squeezing raw leaves and stems of chishima zasa (also known as nematode mushroom) (Patent Document 7).

 Patent Document 1: Japanese Patent Application Laid-Open No. 2004-337151

 Patent Document 2: Japanese Patent Application Laid-Open No. 2004-359698

 Patent Document 3: Japanese Patent Laid-Open No. 9-278662

 Patent Document 4: Japanese Patent Laid-Open No. 11-269020

Patent Document 5: Japanese Unexamined Patent Application Publication No. 2004-161621 Patent Document 6: Japanese Unexamined Patent Application Publication No. 2004-196731

 Patent Document 7: Japanese Patent Application Laid-Open No. 2004-224750

 Disclosure of the invention

 Problems to be solved by the invention

[0006] The present invention is a composition derived from a natural product that can be widely applied in the field of external preparations for skin and foods and drinks, and can be applied to a humectant, a cell activator, a whitening agent, or an antioxidant. The object is to provide a composition.

 Means for solving the problem

 [0007] In order to achieve the above object, the present invention provides a nematode mushroom-containing composition having nematode mushroom as an active ingredient and having moisturizing, cell activation, whitening or antioxidant action.

 [0008] As a result of investigations on various natural products, the present inventors have found that Nemadaridake (Poaceae Sasa, Scientific name: Sara kurilensis), in particular its exudate, has been completely known. It has excellent cell activation effect (anti-aging effect), collagen production promotion effect, antioxidant effect and melanin production inhibitory effect, and it is a moisturizer, cell activator, whitening agent or antioxidant. The present inventors have found that it can be applied as an active ingredient and have completed the present invention.

 [0009] By using Negararidake as an active ingredient in this way, a moisturizer, cell activator, whitening agent or antioxidant can be obtained. As the moisturizer, a moisturizer for application to the skin (a moisturizer for skin) ) Is particularly excellent, and as a cell activator, it can be used particularly effectively as a dermal fibroblast activator. In addition, Negaridae functions as a tyrosinase activity inhibitor, and as a result, whitening effect is considered to be obtained. Negaridae is also effective as a radical scavenger (radical scavenger), and it is thought that an antioxidant effect can be obtained by scavenging free radicals.

 [0010] As described above, the present invention provides use (use method) of Negararidake as a moisturizer, cell activator, whitening agent or antioxidant.

 The invention's effect

[0011] According to the present invention, there is a Negararidake-containing composition that can be widely applied in the fields of external preparations for skin and foods and drinks, and can be applied as a moisturizer, cell activator, whitening agent or antioxidant. Provided. This composition can be used as a moisturizer, cell activator, whitening agent or antioxidant having an excellent effect. In addition, by adding negararidake to a composition such as an external preparation for skin and foods and drinks, it is possible to obtain an excellent effect in preventing and improving skin symptoms such as wrinkles, tarmi, skin firmness, spots, and scum.

 BEST MODE FOR CARRYING OUT THE INVENTION

 [0012] Neakaridake (Sara kurilensis), which is a plant used as a raw material of the present invention, is a plant belonging to the genus Sasa of the family Gramineae. Nemagari-dake (Negakutake) is also known as Chishimazasa, Jitake, etc., and is grown in the Tohoku region and Hokkaido from the Sea of Japan in Honshu, Japan and north of Tottori Prefecture, and is available in these regions. .

 [0013] In the case of using Negararidake for extraction, it can be used after being pulverized as it is. Nemagaritake may use any part of leaves, stems, roots, buds, flowers, etc., or a combination thereof, which may use the whole tissue for extraction. Since the above-described effects are more excellent, young shoots that preferably use shoots as nematode are more preferable. In the extraction, it may be used as it is, but in consideration of the extraction efficiency, the amount of the extracted component, etc., the extraction can also be performed after processing such as shredding, drying, and pulverization.

 [0014] As an extraction method, an extraction method using immersion in an extraction solvent or using a supercritical fluid or a subcritical fluid can be applied. In order to increase the extraction efficiency, the extraction force with stirring may be extracted after homogenizer or a mixer or the like is used to homogenize the raw material in the extraction solvent.

 [0015] The extraction solvent is water; lower alcohols such as methanol, ethanol, propanol, isopropanol (referred to as alcohols having 6 or less carbon atoms; the same shall apply hereinafter); 1,3-butylene glycol, propylene glycol, dipropylene glycol, glycerin Polyhydric alcohols such as: ethers such as ethyl ether and propyl ether; esters such as butyl acetate and ethyl acetate; ketones such as acetone and ethyl methyl ketone can be used, and one or two of them can be used. The above is selected and used. The lower alcohols, polyhydric alcohols, ethers, esters and ketones correspond to polar organic solvents.

[0016] As the extraction solvent, physiological saline, phosphate buffer, phosphate buffered saline, or the like may also be used. In addition, water, carbon dioxide, ethylene, propylene, ethanol, methanol, One or more supercritical fluids and subcritical fluids such as ammonia may be used. That is, supercritical extraction and subcritical extraction may be performed using water, carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia, and the like.

 [0017] The extraction temperature is suitably from about 0 ° C to 5 ° C to below the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is preferably about 1 hour to 14 days.

 [0018] The extraction may be performed at normal temperature (room temperature, for example, 10 to 40 ° C) and normal pressure (1 atm = about 1001 ^ ½), or may be performed at a high temperature (for example, 50 to 200 ° C, using an autoclave). (Preferably 50 to 150 ° C.) It is performed at high pressure (for example, more than lOOkPa and less than 500 kPa, preferably more than lOOkPa and less than 300 kPa).

 [0019] When performing supercritical extraction or subcritical extraction, the critical temperature and critical pressure are determined according to the medium used. For example, when the medium is carbon dioxide, the critical temperature is 31 ° C, the critical pressure is 7.3 MPa, when the medium is methanol, the critical temperature is 239 ° C, the critical pressure is 8. lMPa, and when the water is water, the critical temperature is 374 ° C, Critical pressure 22. Can be set to IMPa.

 [0020] Particularly preferred as the extraction of the oyster mushroom is extraction with a lower alcohol aqueous solution (for example, a methanol aqueous solution or an ethanol aqueous solution, particularly an ethanol aqueous solution) at a normal temperature and normal pressure, and a high temperature (for example, 50 to 200 ° C, preferably 50 (~ 150 ° C, in particular 120 ° C) extraction with water under caloric pressure. By performing such extraction, an extract having an excellent function as a moisturizer, cell activator, whitening agent or antioxidant can be obtained effectively and reliably.

 [0021] The extract of Nemagari-dake with the above solvent can be used as it is. The concentrated, dried product can be used again by dissolving it in water or a polar solvent. Further, it may be used after performing purification treatment such as decolorization, deodorization, desalting, etc. and fractionation treatment by column chromatography etc. within the range not impairing these physiological functions. The above-mentioned extract of Negaridae, its processed products and fractions can be lyophilized after each processing and fractionation, and dissolved in a solvent before use.

[0022] An extract of Negaridae has an excellent moisturizing action, cell activation action, whitening action, and antioxidant action, and the moisturizing agent, cell activator, whitening agent, antioxidant, external preparation for skin, and food and drink Goods Can be used as In addition, moisturizers, cell activators, whitening agents, and antioxidants, which are made from the extract of Negararidake, can be used for hair that is not only applied to the skin but also can be taken orally. It can also be applied to pharmaceuticals.

 [0023] A moisturizing agent containing an extract of Nemadaritake mushroom as an active ingredient exhibits an excellent moisturizing action on the skin and hair, and particularly has a high moisturizing effect on the skin.

[0024] A cell activator comprising an extract of Nemadaritake as an active ingredient exhibits an excellent activation effect on various cells, but particularly exhibits an excellent effect on dermal fibroblasts.

[0025] A whitening agent containing an extract of Nemagari-dake is effective for improving pigmentation symptoms such as stains and freckles, and is particularly effective for suppressing melanin production based on inhibition of tyrosinase activity. To do.

[0026] An antioxidant comprising an extract of Negaridae as an active ingredient exerts an excellent effect on the ability to exert an excellent antioxidant action, particularly a free radical scavenging action (action as a radical scavenger).

 [0027] In addition, by incorporating Negararidake extract into an external preparation for skin, it exhibits an excellent effect in preventing and improving skin symptoms such as wrinkles, tarmi, skin firmness, spots, Tasumi, dryness, and fine wrinkles. A skin external preparation can be obtained, and can also be used as a skin external preparation for improving anti-aging or a skin external preparation for whitening. Furthermore, the extract of Negararidake can also be used in foods and beverages for the purpose of beauty, health maintenance or nutritional supplementation.

[0028] The amount of Negararidake extract to be added to a skin external preparation as a moisturizer, cell activator, whitening agent, or antioxidant should be adjusted according to the type of skin external preparation, purpose of use, etc. but it is, in terms of effect and stability, 0001-50. it is preferably 0 wt% 0.1 of the total amount, more preferably 0. 001-25. 0 wt ^_0/0.

 [0029] The dosage form of the external preparation for skin (humectant, cell activator, whitening agent, anti-oxidant agent, etc.) containing the extract of Negararidake is arbitrary, for example, soluble wrinkles such as lotion, It can be provided as a dispersion system such as an emulsification system such as cream or emulsion, or a calamine lotion. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders, granules filled with propellants.

[0030] It should be noted that the extract of Negaridae is used as a moisturizer, cell activator, whitening agent, antioxidant, etc. In addition to Negararidake extract, oil preparations and moisturizing agents are usually included in pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics and cleansing agents as needed. Agents, powders, pigments, emulsifiers, solubilizers, detergents, ultraviolet absorbers, thickeners, drugs, fragrances, scabs, antibacterial / antifungal agents, alcohols and the like can be appropriately blended. In addition, other cell activators, whitening agents, and antioxidants can be used in combination as long as the effects of the present invention are not impaired.

 Example

 [0031] In the following, the production example of the extract of the oyster mushroom, the test for evaluating each action, the prescription example as a skin external preparation and food, and the use test will be described in more detail. It is not limited at all!

 [Production Example 1]

 20 times the amount of 50% by weight ethanol aqueous solution was added to dried pulverized larvae of Nemagari-dake and extracted for 2 hours while stirring at room temperature. Insoluble matter was removed by filtration to obtain an extract. The extract was concentrated under reduced pressure, and then freeze-dried to obtain an extract of Negaridae.

[Production Example 2]

 20 times the amount of purified water was added to the dried pulverized larvae of Nemagaridake, and the mixture was heated and extracted at 120 ° C for 20 minutes using an autoclave. While maintaining the high temperature, the insoluble matter was removed by suction filtration to obtain an extract. The extract was concentrated under reduced pressure, and then freeze-dried to obtain an extract of Negaridae.

 [0034] Various evaluation tests were conducted using the extract of the oyster mushroom obtained by the above production example.

 [Example 1] Evaluation experiment of dermal cell activation action

In this evaluation experiment, a sample obtained according to the production method described in Production Example 1 was used. The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in 96-well microphone mouthplates at 2.0 × 10 ⁴ per well. As the seeding medium, Dulbecco's modified Eagle medium (DMEM) supplemented with 1% by weight of urine fetal serum (FBS) was used. After culturing for 24 hours, the medium was replaced with a sample prepared at each concentration in DMEM medium supplemented with 1% by mass FBS, and further cultured for 48 hours. [0036] Next, the medium was replaced with a medium containing 400 μgZmL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) and cultured for about 2 hours. Formazan produced by the opening of the tetrazolium ring was extracted with 2-propanol. The absorbance at 550 nm was measured with a microplate reader, and at the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in DMEM medium (blank) with 1% by mass FBS added without sample as 100. In the table, ** indicates a significance probability of less than 1% (P <0.01) with respect to the significance P value in the t test.

 [0037] [Table 1]

 Cell activation on dermal fibroblasts

[0038] As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium supplemented with the extract of Negaridae. In particular, when 0.25 ~: L OmgZmL of the sample was added, significant dermal fibroblast activation was observed at a risk rate of less than 1% compared to the blank. From this, it was clarified that the extract of Nemadaritake has an excellent dermal fibroblast activation effect.

 [0039] [Example 2] Evaluation experiment of type I collagen production promoting action

In this evaluation experiment, a sample obtained according to the production method described in Production Example 1 was used. The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in 96-well microphone mouthplates at 2.0 × 10 ⁴ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 0.5% by weight urchin fetal serum (FBS). After culturing for 24 hours, the medium was replaced with a sample prepared at various concentrations in DMEM medium supplemented with 1% by mass FBS, and further cultured for 24 hours. In this case, 0.5 weight as a negative control (blank) ⁰ / oFBS添Ka卩DMEM medium, 0.5 mass containing the 50mgZmL as a positive control L Asukorubin acid phosphate ester magnesium salt (VCPMg) _^0/0 DMEM medium supplemented with FBS was used.

[0040] The amount of type I collagen secreted into the culture supernatant was measured using an enzyme-linked immunosorbent assay (ELISA). First, type I collagen in the culture supernatant is reacted with a rabbit anti-human type I collagen polyclonal antibody (CHEMICON) and then labeled with a peroxidase-labeled anti-rabbit IgG polyclonal antibody (HISTOFINE; -Chilei) as a secondary antibody. did. Next, the labeled peroxidase was reacted with 2,2,1-azinobis (3-ethylbenzothiazoline 6-sulfonic acid) diammonium salt (ABTS) and hydrogen peroxide, and then reacted with a microplate reader. The absorbance at 405 nm was measured. Furthermore, the amount of protein in each well was measured using the BCA Protein Assay Kit manufactured by PIERCE, and the amount of collagen produced per unit protein was determined. The evaluation results are shown in Table 2 as relative values when the amount of collagen production per unit tank amount of the negative control is 100. In the table, ** indicates a significance probability of less than 1% (P <0.01) with respect to significance P value in t-test.

[0041] [Table 2] Promoting action of type I collagen production on dermal fibroblasts

[0042] As is apparent from Table 2, in the medium supplemented with the extract of Negaridae, a significant type I collagen production promoting effect on dermal fibroblasts was observed. In particular, when the sample was added at 0.5 to 1. OmgZmL, a significant dermal fibroblast activation effect was observed at a risk rate of less than 1% compared to the blank. From this, it has been clarified that the extract of Nemadaritake has an excellent type I collagen production promoting action.

 [0043] [Example 3] Evaluation experiment of antioxidant action (DPPH radical scavenging action)

In this evaluation experiment, samples obtained according to the production methods described in Production Examples 1 and 2 were used. The evaluation was performed according to the following procedure. Using a 50% by weight ethanol aqueous solution Adjusted and added 100 L to a 96-well microphone opening plate. Next, 0.2 / mL of an ethanol solution of 1-diphe-lou 2-picrylhydrazyl (DPPH) was added to a 96-well microphone opening plate at 100 / zL. After thorough mixing, the mixture was allowed to stand in a dark place at room temperature for 10 minutes, and then the absorbance at 516 nm was measured. When the absorbance of the blank with no sample added was (A) and the absorbance when the sample was added was (B), the value obtained by the following formula was taken as the radical elimination rate. The evaluation results are shown in Table 3.

 {1-(B) / (A)} X 100 (%)

[0044] [Table 3]

 Antioxidant action (D P P H radical scavenging ability)

 [0045] As is clear from Table 3, it was found that the extract of Negaridae has an anti-oxidative action based on radical scavenging.

 [Example 4] Evaluation experiment of inhibitory action on melanin production using B16 melanoma

 In this evaluation experiment, a sample obtained according to the production method described in Production Example 2 was used. The evaluation was performed according to the following procedure. B16 mouse melanoma (B16F0) cells were seeded at a density of 2000 cells per dish in a 35 mm dish. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight urine fetal serum (FBS). After culturing for 24 hours, the medium was changed to a medium adjusted to each concentration with 5% by mass FBS-added DMEM medium, and further cultured for 7 days. At this time, the sample is 5 masses with no additive. / oFBS-added DMEM medium is a negative control (blank) and contains 5 mass of sodium lactate at a concentration of 50 mM. / oFBS supplemented DMEM medium was used as a positive control.

[0047] After completion of the culture, the cells were collected using 0.25% trypsin, transferred to a 1.5 mL microtube, and centrifuged to obtain a cell precipitate. The resulting precipitate was visually judged based on Table 4 with respect to its blackish state. At this time, negative control was evaluated as 5 and positive control was evaluated as 1, which was used as an index for determining the sample-added medium. Also obtained above The tissue solubilizer (trade name: Solvable) was added to the precipitate and boiled, then cooled to room temperature, and the absorbance at 500 nm was measured. Table 5 shows the determination results and the absorbance at 500 nm.

 [Table 4]

 Table 4 Judgment and evaluation words

[0049] [Table 5]

Inhibition of melanin production using B 16 melanoma

 [0050] As can be seen from Table 5, when using a medium supplemented with lOOmgZmL of the sample, no black wrinkles were observed, and the nematode mushroom extract has an excellent inhibitory effect on melanin production. It became.

 [0051] Next, a formulation example of a skin external preparation (skin external preparation applicable as a moisturizing agent, a cell activator, a whitening agent, an antioxidant, etc.) formulated with the extract of the oyster mushroom according to the present invention will be shown. Hereinafter, unless otherwise specified, the amount of each component means mass%.

 [0052] [Prescription Example 1] Emulsion

 (1) Screen 10.0

 (2) Methylphenol polysiloxane 4.0

 (3) Hydrogenated palm kernel oil 0.5

 (4) Hydrogenated soybean phospholipid 0.1

(5) Polystearic acid polyoxyethylene sorbitan (20E. O.) (6) Sorbitan monostearate1.0

 (7) Glycerin 4.0

 (8) Methyl paraoxybenzoate 0.1

 (9) Canoleboxyvininole polymer 0.15

 (10) Purified water 53. 85

 (11) Arginine (1 mass% aqueous solution) 20. 0

 (12) Negararidake extract (Production Example 1) 5.0

 Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this while stirring and emulsified uniformly with a homogenizer. After emulsification, start cooling and add (11) and (12) in order and mix uniformly.

[0053] [Prescription Example 2] lotion

 (1) Ethanol 15.0

 (2) Polyoxyethylene (40E. O.) hydrogenated castor oil

 0. 3

 (3) Fragrance 0. 1

 (4) Purified water 78. 38

 (5) Chenic acid 0.02

 (6) Sodium quenate 0.1

 (7) Glycerin 1.0

 (8) Hydroxyetinoresenorelose 0.1

 (9) Negararidake extract (Production Example 2) 5.0

 Production method: Dissolve (2) and (3) in (1). After dissolution, add (4) to (8) sequentially, and then stir well. Add (9) and mix uniformly.

[0054] [Prescription Example 3] Cream

 (1) Screen 10.0

 (2) Stearic acid 2.0

(3) Hydrogenated palm kernel oil 0.5 (4) Hydrogenated soybean phospholipid 0.1

 (5) Cetanol 3.6

 (6) Lipophilic glyceryl monostearate2.0

 (7) Glycerin 10.0

 (8) Methyl paraoxybenzoate 0.1

 (9) Arginine (20% by weight aqueous solution) 15. 0

 (10) Purified water 40.7

 (11) Carboxyvinyl polymer (1% by weight aqueous solution)

 15. 0

 (12) Negararidake extract (Production Example 1) 1.0

 Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this while stirring and emulsified uniformly with a homogenizer. After emulsification, add (11), start cooling, add (12) at 40 ° C and mix uniformly.

[Prescription Example 4] Essence

 (1) Purified water 27. 45

 (2) Glycerin 10.0

 (3) Sucrose fatty acid ester 1.3

 (4) Carboxyvinyl polymer (1% by weight aqueous solution)

 17. 5

 (5) Sodium alginate (1% by weight aqueous solution)

 15. 0

 (6) Polyglyceryl monolaurate 1.0

 (7) Macadamia nut oil fatty acid phytosteryl

 3. 0

 (8) N-lauroyl L di (phytosteryl) glutamate

 2. 0

(9) Hardened palm oil 2.0 (10) Sukuran (Olive) 1.0

 (11) Beheal alcohol 0.75

 (12) Millow 1.0

 (13) Jojoba oil1.0

 (14) 1, 3-Butylene glycol 10. 0

 (15) L-Arginine (10% by weight aqueous solution) 2.0

 (16) Negararidake extract (Production Example 2) 5.0

 Production method: Mix the aqueous phase components (1) to (6) and dissolve at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, after preliminarily emulsifying by adding the oil phase component to the aqueous phase component, the mixture is uniformly emulsified with a homomixer. Start cooling after emulsification and cover (15) at 50 ° C. Cool to 40 ° C, add (16), and mix evenly.

[0056] [Prescription Example 5] Aqueous gel

 (1) Canoleboxyvininole polymer 0.5

 (2) Purified water 86.7

 (3) Sodium hydroxide (10% by weight aqueous solution) 0.5

 (4) Ethanol 10.0

 (5) Methyl paraoxybenzoate 0.1

 (6) Fragrance 0. 1

 (7) Negararidake extract (Production Example 2) 2.0

 (8) Polyoxyethylene (60E. O.) hydrogenated castor oil

 0. 1

 Manufacturing method: Add (1) to (2), stir uniformly, and then add (3). After stirring uniformly, add (5) previously dissolved in (4). After stirring uniformly, add (6) to (8) previously mixed and stir and mix uniformly.

[0057] [Prescription Example 6] Cleansing Fee

 (1) Sukuran 81.0

(2) Polyoxyethylene glyceryl isostearate (3) Purified water 3.0

 (4) Negararidake extract (Production Example 1) 1.0

 Manufacturing method: Dissolve (1) and (2) uniformly. Add (3) and (4) to this, and mix evenly.

[0058] [Prescription Example 7] Face-wash form

 (1) Stearic acid 16.0

 (2) Myristic acid 16.0

 (3) Lipophilic glyceryl monostearate2.0

 (4) Glycerin 20.0

 (5) Sodium hydroxide 7.5

 (6) Palm oil fatty acid amidopropyl betaine 1.0

 (7) Purified water 36.5

 (8) Negararidake extract (Production Example 2) 1.0

 Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the water phase components (5) to (7) are heated and dissolved at 80 ° C, and mixed with the oil phase components uniformly. Start cooling, add (8) at 40 ° C, and mix uniformly.

[0059] [Prescription Example 8] Make-up base cream

 (1) Screen 10.0

 (2) Cetanol 2.0

 (3) Glycerol tri-2-ethylhexanoate

 twenty five

 (4) Lipophilic glyceryl monostearate 1.0

 (5) Propylene glycol 11.0

 (6) Sucrose fatty acid ester 1.3

 (7) Purified water 69.4

 (8) Titanium oxide 1.0

(9) Bengala 0.1 (10) Yellow iron oxide 0.4

 (11) Fragrance 0. 1

 (12) Negararidake extract (Production Example 1) 1.2

 Manufacturing method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed, dissolved by heating at 75 ° C, and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Start cooling after emulsification, add ingredients (11) and (12) at 40 ° C, and mix evenly.

 Formulation Example 9] Milk Foundation

 1) Methylenopolysiloxane 2.0

 2) Screening 5.0

 3) Otachidodecyl myristate 5.0

 4) Cetanore 1.0

 5) Polyoxyethylene sorbitan (20E. O.) monostearate

 13

 6) Sorbitan monostearate 0.7

 7) 1,3-Butyleneglycolanol 8.0

 8) Xanthan gum 0.1

 9) Methyl paraoxybenzoate 0.1

 10) Purified water 57.4

 11) Titanium oxide 9.0

 12) Talc 7.4

 13) Bengala 0.5

 14) Yellow iron oxide 1.1

 15) Black iron oxide 0.1

 16) Fragrance 0. 1

 17) Negararidake extract (Production Example 2) 1.0

Manufacturing method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, (7)-(10) The aqueous phase components are mixed and dissolved by heating at 75 ° C, and the pigments (11) to (15) are added thereto and dispersed uniformly with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after emulsification, and components (16) and (17) are added sequentially at 40 ° C and mixed uniformly.

[0061] [Prescription Example 10] Water-in-oil emollient cream

 (1) Liquid paraffin 30. 0

 (2) Microcrystalline wax 2.0

 (3) Vaseline 5.0

 (4) Diglycerinoleate 5.0

 (5) Sodium salt sodium 1.3

 (6) Potassium salt 0.1

 (7) Propylene glycolate 3.0

 (8) 1,3-Butyleneglycolanol 5.0

 (9) Methyl paraoxybenzoate 0.1

 (10) Negararidake extract (Production Example 2) 1.0

 (11) Purified water 47.4

 (12) Fragrance 0. 1

 Manufacturing method: Dissolve (5) and (6) in a part of (11) to 50 ° C, and gradually add to (4) heated to 50 ° C while stirring. After mixing this, disperse uniformly in (1) to (3), which is heated and dissolved at 70 ° C. To this, heat (7) to (10) in the remainder of (11) and dissolve at 70 ° C while stirring and emulsify with a homomixer. Start cooling after emulsification, add (12) at 40 ° C, and mix uniformly.

 [0062] [Prescription Example 11] Pack

 (1) Purified water 58.9

 (2) Posibulal alcohol 12.0

 (3) Ethanol 17.0

 (4) Glycerin 5.0

 (5) Polyethylene glycol (average molecular weight 1000)

2. 0 (6) Negararidake extract (Production Example 1) 5.0

 (7) Fragrance 0. 1

 Manufacturing method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After evenly dissolving, add (4) and (5) and start cooling with stirring. Cool to 40 ° C, add (6) and (7), and mix evenly.

[0063] [Prescription Example 12] bath agent

 (1) Fragrance 0.3

 (2) Negararidake extract (Production Example 2) 1.0

 (3) Sodium bicarbonate 50. 0

 (4) Sodium sulfate 48.7

 Production method: (1) to (4) are mixed uniformly.

 [0064] [Prescription Example 13] Hair wax

 (1) Stearic acid 3.0

 (2) Microcrystalline wax 2.0

 (3) Cetyl alcohol 3.0

 (4) Highly polymerized methylpolysiloxane 2.0

 (5) Methylenopolysiloxane 5.0

 (6) Poly (oxyethylene / oxypropylene) methylpolysiloxane copolymer

 Ten

 (7) Methyl paraoxybenzoate 0.1

 (8) 1,3-Butylene glycol 7.5

 (9) Arginine 0.7

 (10) Purified water 73.6

 (11) Negararidake extract (Production Example 2) 2.0

 (12) Fragrance 0. 1

Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 75 ° C., and the oil phase components are collected and emulsified with a homomixer. Cooling is started after emulsification, and the ingredients (11) and (12) are collected at 40 ° C and evenly mixed. Mix.

 [0065] [Prescription Example 14] Hair art nick

 (1) Ethanol 50.0

 (2) Purified water 48.9

 (3) Negararidake extract (Production Example 1) 1.0

 (4) Fragrance 0.1

 Production method: Components (1) to (4) are mixed and homogenized.

 [0066] [Prescription Example 15] Beverage

 (1) Negararidake extract (Production Example 2) 8.0

 (2) Erythritol 1.0

 (3) Chenic acid 0.1

 (4) Stevia 0. 01

 (5) Purified water 90. 89

 Production method: (1) to (5) are mixed uniformly.

 [0067] [Examples 5 and 6 and Comparative Example 1] Usage test

 A use test was conducted using a prescription containing Nemagaridake extract, and the effect of improving skin roughness due to drying was evaluated. At that time, the nematode mushroom extract shown in Table 6 was blended in the emulsion formulation shown in Formulation Example 1, and the use test was conducted as Examples 5 and 6. In addition, the nematode mushroom extract was replaced with purified water, and a use test was conducted simultaneously as Comparative Example 1.

 [0068] [Table 6]

For each sample, 20 female panelists with dry skin in their 30s to 50s who have markedly rough skin symptoms are grouped together and used blindly for 1 week to observe changes in skin condition before and after use. evaluated. As an indicator of skin symptoms, skin roughness due to dryness was evaluated in three stages: “Improved”, “Slightly improved”, and “No change”. Shown in number.

[0070] [Table 7]

 [0071] From Table 7, in the comparative example use group that does not contain the nematode mushroom extract, 80% of the panelists did not improve, but in the example use group containing the nematode mushroom extract, Over 60% of panelists showed clear improvement in rough skin. From this, it has been clarified that the extract of the oyster mushroom has an excellent moisturizing effect.

 Industrial applicability

[0072] According to the present invention, it is possible to widely apply to the field of external preparations for skin, foods and drinks, etc., and a composition containing negaridae can be applied as a moisturizer, cell activator, whitening agent or antioxidant. The This composition can be used as a moisturizer, cell activator, whitening agent or antioxidant having an excellent effect. In addition, by adding Negararidake to a composition such as an external preparation for skin and foods and drinks, it is possible to obtain an excellent effect in preventing and improving skin symptoms such as wrinkles, tarmi, skin firmness, spots, and kusumi.

Claims

The scope of the claims  [1] A composition containing nematode mushrooms containing nematode mushroom as an active ingredient and having moisturizing, cell activation, whitening or antioxidant action.  [2] A moisturizing agent containing Negararidake as an active ingredient. [3] A cell activator comprising Negararidake as an active ingredient. [4] Whitening agent containing Negararidake as an active ingredient. [5] Antioxidant containing Negararidake as an active ingredient.