[go: up one dir, main page]

WO2008016958B1 - Reproductive cell media compositions and methods for improving fertility - Google Patents

Reproductive cell media compositions and methods for improving fertility

Info

Publication number
WO2008016958B1
WO2008016958B1 PCT/US2007/074935 US2007074935W WO2008016958B1 WO 2008016958 B1 WO2008016958 B1 WO 2008016958B1 US 2007074935 W US2007074935 W US 2007074935W WO 2008016958 B1 WO2008016958 B1 WO 2008016958B1
Authority
WO
WIPO (PCT)
Prior art keywords
composition
reproductive
growth factor
reproductive cell
colostrum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/074935
Other languages
French (fr)
Other versions
WO2008016958A2 (en
WO2008016958A3 (en
Inventor
Ludwig Simmet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Minitube of America Inc
Original Assignee
Minitube of America Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Minitube of America Inc filed Critical Minitube of America Inc
Publication of WO2008016958A2 publication Critical patent/WO2008016958A2/en
Publication of WO2008016958A3 publication Critical patent/WO2008016958A3/en
Publication of WO2008016958B1 publication Critical patent/WO2008016958B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0604—Whole embryos; Culture medium therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Disclosed are compositions for reproductive cells which comprise a suitable reproductive cell media and a milk- or colostrum-derived product. The milk- or colostrum-derived product may comprise a growth factor, such as a transforming growth factor or insulin-like growth factor, or α-lactalbumin. The compositions may be optionally substantially free of bovine serum derived products. Methods for improving fertility in animals using compositions of the invention are also disclosed.

Claims

AMENDED CLAIMS received by the International Bureau on 11 March 2008 (11.03.2008)CLAIMSWe claim:
1. A composition comprising a reproductive cell medium, a milk- or colostrum- derived growth factor, and α-lactalbumin.
2. The composition of claim 1 , wherein the growth factor comprises IGF-1.
3. The composition of claim 1 , wherein the growth factor comprises TGF-β2.
4. The composition of claim 1, comprising milk- or colostrum-derived IGF-1 and milk- or colostrum-derived TGF-β2.
5. The composition of claim 4, wherein the ratio of IGF-1 to TGF-β2 is from about 5:1 to about 2:1.
6. Canceled
7. The composition of any of claims 1 to 5, wherein the reproductive cell medium is a sperm cell medium.
8. The composition of any of claims 1 to 5 and 7, wherein the reproductive cell medium is reproductive mammalian cell medium.
9. The composition of any of claims 1 to 5 and 7-8, wherein the composition is substantially free of bovine serum-derived products.
10. The composition of any of claims 1 to 5 and 7-9, further comprising a reproductive cell.
11. The composition of claim 10, wherein the reproductive cell is a sperm cell or oocyte.
12. The composition of any of claims 10 to 11, wherein the reproductive cell Is a mammalian cell.
PCT/US2007/074935 2006-08-01 2007-08-01 Reproductive cell media compositions and methods for improving fertility Ceased WO2008016958A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82104406P 2006-08-01 2006-08-01
US60/821,044 2006-08-01

Publications (3)

Publication Number Publication Date
WO2008016958A2 WO2008016958A2 (en) 2008-02-07
WO2008016958A3 WO2008016958A3 (en) 2008-03-20
WO2008016958B1 true WO2008016958B1 (en) 2008-05-08

Family

ID=38997829

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/074935 Ceased WO2008016958A2 (en) 2006-08-01 2007-08-01 Reproductive cell media compositions and methods for improving fertility

Country Status (1)

Country Link
WO (1) WO2008016958A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITRM20100300A1 (en) * 2010-06-03 2011-12-04 Lo Li Pharma Srl FORMULATION OF COMPOUND FOR THE TREATMENT OF SEMINAL MALE LIQUID INTENDED FOR MEDICALLY ASSISTED REPRODUCTION TECHNIQUES
US10279013B2 (en) 2013-12-19 2019-05-07 Puretein Bioscience Llc Methods for treating an animal

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4511558A (en) * 1983-05-16 1985-04-16 University Patents, Inc. Alpha-lactalbumin contraceptive
US6849394B2 (en) * 2002-02-21 2005-02-01 Minitube Of America Compositions comprising reproductive cell media and methods for using such compositions

Also Published As

Publication number Publication date
WO2008016958A2 (en) 2008-02-07
WO2008016958A3 (en) 2008-03-20

Similar Documents

Publication Publication Date Title
Wrenzycki et al. Alterations in the relative abundance of gene transcripts in preimplantation bovine embryos cultured in medium supplemented with either serum or PVA
Baker et al. Using deubiquitylating enzymes as research tools
WO2010102085A4 (en) Stabilized reverse transcriptase fusion proteins
WO2003072707B1 (en) Compositions comprising reproductive cell media and methods for using such compositions
CA2601455C (en) Dimeric and trimeric nucleic acid dyes, and associated systems and methods
BRPI0516572A (en) methods and compositions for improving the production of recombinant proteins
MY185872A (en) Cell culture improvements
EP2426221A3 (en) Recombinase polymerase amplification
CA3049825C (en) Cell-free protein synthesis system
CA2474734A1 (en) Thermostable taq polymerase fragment
WO2008027384A8 (en) Methods and compositions for transposon-mediated transgenesis
WO2008016958B1 (en) Reproductive cell media compositions and methods for improving fertility
Guo et al. Obox4 secures zygotic genome activation upon loss of Dux
WO2020053683A1 (en) Process for production of soluble recombinant peptides
WO2006039622A3 (en) Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
WO2008126783A1 (en) Method for producing dipeptide
CA3160450A1 (en) Modular, cell-free protein expression vectors to accelerate biological design in cells
WO2007015691A3 (en) Methods and compositions for increasing longevity and protein yield from a cell culture
Lee et al. Identification of signals required for import of the soybean FAd subunit of ATP synthase into mitochondria
CA3215576A1 (en) Anti-izumo1 immunoglobulins and methods of use thereof
WO2020135623A1 (en) Method for changing in-vitro protein synthesis capability by edc3 gene knockout and application thereof
EP3564367A1 (en) Method of selecting cells
류다영 Enhancing Solubility and Purification Efficiency of Vitronectin in Escherichia coli and Application in Human Pluripotent Stem Cell Culture
WO2005023980A3 (en) Use of complement protein c3 and its derivatives in enhancing mammalian embryo development
Sood et al. Paternal sex chromosome effect on development of buffalo (Bubalus Bubaus) embryos fertilized and cultured In vitro

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07813633

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase in:

Ref country code: DE

NENP Non-entry into the national phase in:

Ref country code: RU

122 Ep: pct application non-entry in european phase

Ref document number: 07813633

Country of ref document: EP

Kind code of ref document: A2