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WO2008009046A8 - Detection of enzymes and microorganisms and devices therefor - Google Patents

Detection of enzymes and microorganisms and devices therefor

Info

Publication number
WO2008009046A8
WO2008009046A8 PCT/AU2007/000991 AU2007000991W WO2008009046A8 WO 2008009046 A8 WO2008009046 A8 WO 2008009046A8 AU 2007000991 W AU2007000991 W AU 2007000991W WO 2008009046 A8 WO2008009046 A8 WO 2008009046A8
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
sample
reaction mixture
substrate
working electrode
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/AU2007/000991
Other languages
French (fr)
Other versions
WO2008009046A1 (en
Inventor
William Graham Fox Ditcham
Simon Andrew Reid
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Environmental Biotechnology CRC Pty Ltd
Original Assignee
Environmental Biotechnology CRC Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2006903837A external-priority patent/AU2006903837A0/en
Application filed by Environmental Biotechnology CRC Pty Ltd filed Critical Environmental Biotechnology CRC Pty Ltd
Publication of WO2008009046A1 publication Critical patent/WO2008009046A1/en
Publication of WO2008009046A8 publication Critical patent/WO2008009046A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/002—Electrode membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to methods for detecting the amount of an enzyme of interest, which may be expressed b a microorganism, in a sample, such methods comprising: (a) incubating a reaction mixture comprising the sample and at least one substrate which can be acted on by the enzyme; (b) contacting an electrochemical sensor with the reaction mixture comprising the sample either: (i) after a selected time of incubation of the reaction mixture or (ii) at the same time as or soon after preparing the reaction mixture, wherein the sensor comprises a working electrode which has immobilised therein or thereto a second enzyme which is capable of acting on a substance selected from one or more of the at least one substrate and a product resulting from action of the enzyme of interest on the at least one substrate; wherein action of the second enzyme on the at least one substrate or product results in a change in electric potential at said working electrode; (c) either: (i) detecting the change in electrical potential at the working electrode after the contacting in step (b)(i); or (ii) determining the period of time required for the electric potential at the working electrode to return to a substantially steady baseline value after the contacting in step (b)(ii); and (d) determining from the change in potential detected at step (c)(i) or period of time determined at step (c)(ii) the amount of enzyme of interest in the sample. The invention also relates to sensors suitable for such methods.
PCT/AU2007/000991 2006-07-17 2007-07-17 Detection of enzymes and microorganisms and devices therefor Ceased WO2008009046A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2006903837 2006-07-17
AU2006903837A AU2006903837A0 (en) 2006-07-17 Detection of microorganisms and devices therefor

Publications (2)

Publication Number Publication Date
WO2008009046A1 WO2008009046A1 (en) 2008-01-24
WO2008009046A8 true WO2008009046A8 (en) 2008-03-06

Family

ID=38956422

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2007/000991 Ceased WO2008009046A1 (en) 2006-07-17 2007-07-17 Detection of enzymes and microorganisms and devices therefor

Country Status (2)

Country Link
TW (1) TW200817520A (en)
WO (1) WO2008009046A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009009292A1 (en) * 2009-02-17 2010-08-26 Siemens Aktiengesellschaft Diagnostic device for detection of helicobacter pylori for treatment of stomach illness, has electrodes dipped into container filled with acidic nutrient solution, where potential is measurable between electrodes
US20120149046A1 (en) * 2009-08-20 2012-06-14 James Paul Meighan Bioluminescent Bacterial Detection
US10408783B2 (en) 2014-05-30 2019-09-10 Georgia State University Research Foundation, Inc. Electrochemical methods and compounds for the detection of enzymes
DK3183362T3 (en) 2014-08-22 2021-06-28 Univ Nanyang Tech Electrochemical detection of microorganisms
US11567012B2 (en) * 2018-12-31 2023-01-31 Robert Bosch Gmbh Mold detecting device using sensor to measure pH of a surface

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040092004A1 (en) * 2002-11-07 2004-05-13 Hrl Laboratories, Llc Sensor for detection of enzyme and enzyme detection method for aerosolized bacteria in the enviromnment

Also Published As

Publication number Publication date
TW200817520A (en) 2008-04-16
WO2008009046A1 (en) 2008-01-24

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