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WO2008008256A3 - Methods for enhancing production of isoprenoid compounds by host cells - Google Patents

Methods for enhancing production of isoprenoid compounds by host cells Download PDF

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Publication number
WO2008008256A3
WO2008008256A3 PCT/US2007/015498 US2007015498W WO2008008256A3 WO 2008008256 A3 WO2008008256 A3 WO 2008008256A3 US 2007015498 W US2007015498 W US 2007015498W WO 2008008256 A3 WO2008008256 A3 WO 2008008256A3
Authority
WO
WIPO (PCT)
Prior art keywords
host cells
igr
synthetic
methods
biosynthetic pathway
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/015498
Other languages
French (fr)
Other versions
WO2008008256A2 (en
Inventor
Jay D Keasling
Douglas J Pitera
Chris J Paddon
Brian Pfleger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of WO2008008256A2 publication Critical patent/WO2008008256A2/en
Anticipated expiration legal-status Critical
Publication of WO2008008256A3 publication Critical patent/WO2008008256A3/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides methods of producing an isoprenoid or an isoprenoid precursor in a host cell that comprises a biosynthetic pathway that converts a substrate to isopentenyl pyrophosphate, where the biosynthetic pathway is modified to include a synthetic intergenic region (IGR) between at least two coding regions encoding enzymes in the biosynthetic pathway. The present invention further provides recombinant nucleic acid constructs comprising a synthetic IGR, and genetically modified host cells comprising a synthetic IGR.
PCT/US2007/015498 2006-07-07 2007-07-03 Methods for enhancing production of isoprenoid compounds by host cells Ceased WO2008008256A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81970606P 2006-07-07 2006-07-07
US60/819,706 2006-07-07

Publications (2)

Publication Number Publication Date
WO2008008256A2 WO2008008256A2 (en) 2008-01-17
WO2008008256A3 true WO2008008256A3 (en) 2009-04-09

Family

ID=38923792

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/015498 Ceased WO2008008256A2 (en) 2006-07-07 2007-07-03 Methods for enhancing production of isoprenoid compounds by host cells

Country Status (1)

Country Link
WO (1) WO2008008256A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011146833A1 (en) * 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
SG186127A1 (en) 2010-06-02 2013-01-30 Evolva Nutrition Inc Recombinant production of steviol glycosides
EP3792350A1 (en) 2011-08-08 2021-03-17 Evolva SA Recombinant production of steviol glycosides
US9719122B2 (en) 2012-07-18 2017-08-01 Yeda Research And Development Co. Ltd. Methods of production of products of metabolic pathways
MY190346A (en) 2013-02-06 2022-04-15 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
MY184253A (en) 2013-02-11 2021-03-29 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
KR20170041209A (en) 2014-08-11 2017-04-14 에볼바 에스아 Production of steviol glycosides in recombinant hosts
EP4592304A3 (en) 2014-09-09 2025-11-05 Danstar Ferment Ag Production of steviol glycosides in recombinant hosts
BR112017016338A2 (en) 2015-01-30 2018-03-27 Evolva Sa production of steviol glycosides in recombinant hosts
US10604743B2 (en) 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
US10837041B2 (en) 2015-08-07 2020-11-17 Evolva Sa Production of steviol glycosides in recombinant hosts
CN109195457A (en) 2016-04-13 2019-01-11 埃沃尔瓦公司 Steviol glycoside is generated in the recombination host
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
US11365417B2 (en) * 2017-09-12 2022-06-21 Bio Capital Holdings, LLC Biological devices and methods of use thereof to produce steviol glycosides
JP2021505154A (en) 2017-12-07 2021-02-18 ザイマージェン インコーポレイテッド Designed biosynthetic pathway for producing (6E) -8-hydroxygeraniol by fermentation
WO2019126778A1 (en) 2017-12-21 2019-06-27 Zymergen Inc. Nepetalactol oxidoreductases, nepetalactol synthases, and microbes capable of producing nepetalactone

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030148479A1 (en) * 2001-12-06 2003-08-07 Jay Keasling Biosynthesis of isopentenyl pyrophosphate
US20050266518A1 (en) * 2001-06-06 2005-12-01 Dsm Ip Assets B.V. Isoprenoid production

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050266518A1 (en) * 2001-06-06 2005-12-01 Dsm Ip Assets B.V. Isoprenoid production
US20030148479A1 (en) * 2001-12-06 2003-08-07 Jay Keasling Biosynthesis of isopentenyl pyrophosphate

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHEN ET AL., vol. ., 31 May 2006 (2006-05-31) *
DATABASE GENBANK Database accession no. Dx769213 *
MARTIN ET AL.: "Engineering a mevalonate pathway in Escherichia coli for production of terpenoids", NATURE BIOTECHNOLOGY, vol. 21, no. 7, July 2003 (2003-07-01), pages 796 - 802, XP002420804, DOI: doi:10.1038/nbt833 *
PFLEGER ET AL.: "Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes", NATURE BIOTECHNOLOGY, vol. 24, no. 8, August 2006 (2006-08-01), pages 1027 - 1032, XP055046133, DOI: doi:10.1038/nbt1226 *
SMOLKE ET AL.: "mRNA Secondary Structures, and RNase Sites on Expression of Two Genes in an Engineered Operon Biotechnol Bioeng", EFFECT OF GENE LOCATION, vol. 80, 2002, pages 762 - 776 *

Also Published As

Publication number Publication date
WO2008008256A2 (en) 2008-01-17

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