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WO2008002725A3 - Purification and amplification of nucleic acids in a microfluidic device - Google Patents

Purification and amplification of nucleic acids in a microfluidic device Download PDF

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Publication number
WO2008002725A3
WO2008002725A3 PCT/US2007/068937 US2007068937W WO2008002725A3 WO 2008002725 A3 WO2008002725 A3 WO 2008002725A3 US 2007068937 W US2007068937 W US 2007068937W WO 2008002725 A3 WO2008002725 A3 WO 2008002725A3
Authority
WO
WIPO (PCT)
Prior art keywords
amplification
purification
nucleic acids
microfluidic device
reservoir
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/068937
Other languages
French (fr)
Other versions
WO2008002725A2 (en
Inventor
Luis Ugozzoli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of WO2008002725A2 publication Critical patent/WO2008002725A2/en
Publication of WO2008002725A3 publication Critical patent/WO2008002725A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Purification of either DNA or RNA and amplification through PCR or reverse- transcriptase PCR are performed in a common microfluidics device by the inclusion of diatomaceous earth in a reservoir within the device and by the use of microfluidics technology for conveying fluids through the reservoir and through microchannels within the device.
PCT/US2007/068937 2006-06-29 2007-05-15 Purification and amplification of nucleic acids in a microfluidic device Ceased WO2008002725A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/478,953 2006-06-29
US11/478,953 US20080003585A1 (en) 2006-06-29 2006-06-29 Purification and amplification of nucleic acids in a microfluidic device

Publications (2)

Publication Number Publication Date
WO2008002725A2 WO2008002725A2 (en) 2008-01-03
WO2008002725A3 true WO2008002725A3 (en) 2008-02-28

Family

ID=38846368

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/068937 Ceased WO2008002725A2 (en) 2006-06-29 2007-05-15 Purification and amplification of nucleic acids in a microfluidic device

Country Status (2)

Country Link
US (1) US20080003585A1 (en)
WO (1) WO2008002725A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9370506B2 (en) 2008-10-28 2016-06-21 The Board Of Trustees Of The Leland Stanford Junior University Modulators of aldehyde dehydrogenase and methods of use thereof

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3260556B1 (en) * 2006-05-31 2019-07-31 Sequenom, Inc. Methods for the extraction of nucleic acid from a sample
EP2126574B1 (en) 2007-03-08 2015-12-23 The Board of Trustees of the Leland Stanford Junior University Mitochondrial aldehyde dehydrogenase-2 modulators and methods of use thereof
EP2337563B1 (en) 2008-09-08 2014-04-09 The Board of Trustees of The Leland Stanford Junior University Modulators of aldehyde dehydrogenase activity and methods of use thereof
WO2010115016A2 (en) 2009-04-03 2010-10-07 Sequenom, Inc. Nucleic acid preparation compositions and methods
US10457659B2 (en) 2011-04-29 2019-10-29 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for increasing proliferation of adult salivary stem cells
EP2954054B1 (en) 2013-02-08 2018-12-05 Qiagen GmbH Method for separating dna by size
WO2014160185A2 (en) 2013-03-14 2014-10-02 The Board Of Trustees Of The Leland Stanford Junior University Mitochondrial aldehyde dehydrogenase-2 modulators and methods of use thereof
JP6190352B2 (en) * 2014-12-19 2017-08-30 株式会社神戸製鋼所 Fluid distribution device and operation method thereof
DE102017206155A1 (en) * 2017-04-11 2018-10-11 Robert Bosch Gmbh Desorption of nucleic acids

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5658548A (en) * 1993-08-30 1997-08-19 Promega Corporation Nucleic acid purification on silica gel and glass mixtures

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
DE4432654C2 (en) * 1994-09-14 1998-03-26 Qiagen Gmbh Process for the isolation of nucleic acids from natural sources
US6168948B1 (en) * 1995-06-29 2001-01-02 Affymetrix, Inc. Miniaturized genetic analysis systems and methods
US5942443A (en) * 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US7033474B1 (en) * 1997-04-25 2006-04-25 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
US6857449B1 (en) * 1998-01-20 2005-02-22 Caliper Life Sciences, Inc. Multi-layer microfluidic devices
US20050202470A1 (en) * 2000-11-16 2005-09-15 Caliper Life Sciences, Inc. Binding assays using molecular melt curves
US20060062696A1 (en) * 2001-07-27 2006-03-23 Caliper Life Sciences, Inc. Optimized high throughput analytical systems
US6748975B2 (en) * 2001-12-26 2004-06-15 Micralyne Inc. Microfluidic valve and method of manufacturing same
US6877528B2 (en) * 2002-04-17 2005-04-12 Cytonome, Inc. Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US20050042639A1 (en) * 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
US20040121336A1 (en) * 2002-12-20 2004-06-24 Greenfield I Lawrence Method for generating multiple samples containing a predetermined amount of nucleic acid
US9766216B2 (en) * 2003-04-14 2017-09-19 Wako Pure Chemical Industries, Ltd. Reduction of migration shift assay interference
WO2006026602A2 (en) * 2004-08-30 2006-03-09 Rosalind Franklin University Of Medicine And Science Alternatively spliced pre-mrna transcripts in neurodegenerative disease

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5658548A (en) * 1993-08-30 1997-08-19 Promega Corporation Nucleic acid purification on silica gel and glass mixtures
US5658548C1 (en) * 1993-08-30 2001-07-24 Promega Corp Nucleic acid purification on silica geland glass mixtures

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9370506B2 (en) 2008-10-28 2016-06-21 The Board Of Trustees Of The Leland Stanford Junior University Modulators of aldehyde dehydrogenase and methods of use thereof

Also Published As

Publication number Publication date
US20080003585A1 (en) 2008-01-03
WO2008002725A2 (en) 2008-01-03

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