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WO2008096370A3 - An efficient and novel purification method of recombinant hg-csf - Google Patents

An efficient and novel purification method of recombinant hg-csf Download PDF

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Publication number
WO2008096370A3
WO2008096370A3 PCT/IN2008/000069 IN2008000069W WO2008096370A3 WO 2008096370 A3 WO2008096370 A3 WO 2008096370A3 IN 2008000069 W IN2008000069 W IN 2008000069W WO 2008096370 A3 WO2008096370 A3 WO 2008096370A3
Authority
WO
WIPO (PCT)
Prior art keywords
csf
protein
recombinant
efficient
novel purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IN2008/000069
Other languages
French (fr)
Other versions
WO2008096370A2 (en
Inventor
Venkata Krishna Rao Dasari
Kali Satya Bhujanga Rao Adibhatla
Chowdary Nannapaneni Venkaiah
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Natco Pharma Ltd
Original Assignee
Natco Pharma Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Natco Pharma Ltd filed Critical Natco Pharma Ltd
Publication of WO2008096370A2 publication Critical patent/WO2008096370A2/en
Publication of WO2008096370A3 publication Critical patent/WO2008096370A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention relates to Recombinant human Granulocyte Colony Stimulating Factor (G-CSF) and describes a novel purification process for a simple, economically feasible, largely scalable production of pharmaceutically acceptable grade product with high recovery of rhG-CSF expressed from bacterial cells. The invention comprises of the conditions for isolation of protein inclusion bodies; complete refolding in to native form, purification of the protein by two-step chromatography and diafiltration. The end product of this invention viz. high purity G-CSF is suitable for direct injectable use. The efficiency of the protein recovery is 52% with more than 99% of monomer purity.
PCT/IN2008/000069 2007-02-05 2008-02-04 An efficient and novel purification method of recombinant hg-csf Ceased WO2008096370A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN255/CHE/2007 2007-02-05
IN255CH2007 2007-02-05

Publications (2)

Publication Number Publication Date
WO2008096370A2 WO2008096370A2 (en) 2008-08-14
WO2008096370A3 true WO2008096370A3 (en) 2008-12-31

Family

ID=39682192

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2008/000069 Ceased WO2008096370A2 (en) 2007-02-05 2008-02-04 An efficient and novel purification method of recombinant hg-csf

Country Status (1)

Country Link
WO (1) WO2008096370A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8703123B2 (en) 2010-03-17 2014-04-22 Biogenerix Gmbh Method for obtaining biologically active recombinant human G-CSF

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2251025A1 (en) * 2009-05-12 2010-11-17 Universitat Autònoma De Barcelona Use of inclusion bodies as therapeutic agents
JP5808323B2 (en) 2009-06-22 2015-11-10 アムジエン・インコーポレーテツド Protein refolding using chemically controlled redox states
EP3660032B1 (en) 2009-06-25 2025-10-22 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
PL2341061T3 (en) * 2009-12-31 2014-09-30 Arven Ilac Sanayi Ve Ticaret As A novel process for preparing G-CSF (granulocyte colony stimulating factor)
RU2487885C2 (en) * 2010-07-20 2013-07-20 Зао "Биокад" Method for large-scale production, separation and purification of recombinant human granulocyte colony-stimulating factor
CA2807552A1 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
EP3431485B2 (en) 2010-10-01 2024-09-04 ModernaTX, Inc. Engineered nucleic acids and methods of use thereof
WO2012135805A2 (en) 2011-03-31 2012-10-04 modeRNA Therapeutics Delivery and formulation of engineered nucleic acids
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
EP2763701B1 (en) 2011-10-03 2018-12-19 Moderna Therapeutics, Inc. Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
KR20140102759A (en) 2011-12-16 2014-08-22 모더나 세라퓨틱스, 인코포레이티드 Modified nucleoside, nucleotide, and nucleic acid compositions
HUP1200171A1 (en) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Methods for the production of polypeptides
HUP1200172A2 (en) * 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
DE18200782T1 (en) 2012-04-02 2021-10-21 Modernatx, Inc. MODIFIED POLYNUCLEOTIDES FOR THE PRODUCTION OF PROTEINS ASSOCIATED WITH DISEASES IN HUMANS
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9878056B2 (en) 2012-04-02 2018-01-30 Modernatx, Inc. Modified polynucleotides for the production of cosmetic proteins and peptides
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
CA2892529C (en) 2012-11-26 2023-04-25 Moderna Therapeutics, Inc. Terminally modified rna
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
EP2978770B1 (en) * 2013-03-29 2019-11-06 Dr. Reddy's Laboratories Limited Refolding of proteins
EA201690675A1 (en) 2013-10-03 2016-08-31 Модерна Терапьютикс, Инк. POLYNUCLEOTES ENCODING THE RECEPTOR OF LOW DENSITY LIPOPROTEINS
EA035448B1 (en) 2014-07-14 2020-06-17 Геннова Биофармасьютикалз Лимитед PROCESS FOR PURIFICATION OF rHu-GCSF
US20180086808A1 (en) 2015-03-16 2018-03-29 Arven Ilac Sanayi Ve Ticaret A.S. A process for preparing g-csf (granulocyte colony stimulating factor)
CN116059396B (en) * 2021-11-04 2025-07-15 西南大学 A method for inclusion of chuanxiong alcohol extract

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987001132A1 (en) * 1985-08-23 1987-02-26 Kirin-Amgen, Inc. Production of pluripotent granulocyte colony-stimulating factor
WO1998014467A1 (en) * 1996-09-30 1998-04-09 Human Genome Sciences, Inc. Method of purifying protein from inclusion bodies
WO2003051922A1 (en) * 2001-12-19 2003-06-26 Lek Pharmaceuticals D.D. Process for the purification and/or isolation of biologically active granulocyte colony stimulating factor
WO2004001056A1 (en) * 2002-06-24 2003-12-31 Dr. Reddy's Laboratories Ltd. Process for preparing g-csf
WO2004015124A1 (en) * 2002-07-31 2004-02-19 Lek Pharmaceuticals D.D. Process for the production of a heterologous protein
EP1630173A2 (en) * 2004-08-27 2006-03-01 Bioceuticals Arzneimittel AG Process for recovering biologically active human G-CSF from inclusion bodies
WO2006097944A2 (en) * 2005-03-17 2006-09-21 Zenotech Laboratories Limited Process for the purification of recombinant granulocyte-colony stimulating factor

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987001132A1 (en) * 1985-08-23 1987-02-26 Kirin-Amgen, Inc. Production of pluripotent granulocyte colony-stimulating factor
WO1998014467A1 (en) * 1996-09-30 1998-04-09 Human Genome Sciences, Inc. Method of purifying protein from inclusion bodies
WO2003051922A1 (en) * 2001-12-19 2003-06-26 Lek Pharmaceuticals D.D. Process for the purification and/or isolation of biologically active granulocyte colony stimulating factor
WO2004001056A1 (en) * 2002-06-24 2003-12-31 Dr. Reddy's Laboratories Ltd. Process for preparing g-csf
WO2004015124A1 (en) * 2002-07-31 2004-02-19 Lek Pharmaceuticals D.D. Process for the production of a heterologous protein
EP1630173A2 (en) * 2004-08-27 2006-03-01 Bioceuticals Arzneimittel AG Process for recovering biologically active human G-CSF from inclusion bodies
WO2006097944A2 (en) * 2005-03-17 2006-09-21 Zenotech Laboratories Limited Process for the purification of recombinant granulocyte-colony stimulating factor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8703123B2 (en) 2010-03-17 2014-04-22 Biogenerix Gmbh Method for obtaining biologically active recombinant human G-CSF

Also Published As

Publication number Publication date
WO2008096370A2 (en) 2008-08-14

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