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WO2008087895A1 - Method for determination of high-density lipoprotein cholesterol, and reagent for the method - Google Patents

Method for determination of high-density lipoprotein cholesterol, and reagent for the method Download PDF

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Publication number
WO2008087895A1
WO2008087895A1 PCT/JP2008/050236 JP2008050236W WO2008087895A1 WO 2008087895 A1 WO2008087895 A1 WO 2008087895A1 JP 2008050236 W JP2008050236 W JP 2008050236W WO 2008087895 A1 WO2008087895 A1 WO 2008087895A1
Authority
WO
WIPO (PCT)
Prior art keywords
density lipoprotein
cholesterol
reaction
determination
buffer solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/050236
Other languages
French (fr)
Japanese (ja)
Inventor
Shinsuke Kimata
Keizo Yoneda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
Original Assignee
Toyobo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd
Priority to JP2008521735A priority Critical patent/JP5332611B2/en
Publication of WO2008087895A1 publication Critical patent/WO2008087895A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

[PROBLEMS] To provide: a method for the determination of a high-density lipoprotein (HDL) cholesterol level in a biological sample such as a serum and a plasma; and a reagent for use in the method. [MEANS FOR SOLVING PROBLEMS] Disclosed is a method for the determination of a cholesterol level in a high-density lipoprotein by an enzymatic reaction, which comprises: a first reaction step for preferentially scavenging cholesterol in a low-density lipoprotein, a very-low-density lipoprotein and chylomicron contained in a sample, wherein the first reaction is carried out in a buffer solution containing a cholesterol esterase, a cholesterol oxidase and a substance capable of inhibiting the reactivities of the enzymes against the high-density lipoprotein; and a second reaction step for determining the cholesterol level in the high-density lipoprotein, wherein the second reaction is carried out in a buffer solution which is the same as the buffer solution used in the first reaction step except that a substance capable of reducing or eliminating the inhibitory activities of the enzymes against cholesterol in the high-density lipoprotein in the first reaction is additionally contained.
PCT/JP2008/050236 2007-01-15 2008-01-11 Method for determination of high-density lipoprotein cholesterol, and reagent for the method Ceased WO2008087895A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008521735A JP5332611B2 (en) 2007-01-15 2008-01-11 Method for measuring cholesterol in high density lipoprotein and its reagent

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-005687 2007-01-15
JP2007005687 2007-01-15

Publications (1)

Publication Number Publication Date
WO2008087895A1 true WO2008087895A1 (en) 2008-07-24

Family

ID=39635899

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/050236 Ceased WO2008087895A1 (en) 2007-01-15 2008-01-11 Method for determination of high-density lipoprotein cholesterol, and reagent for the method

Country Status (2)

Country Link
JP (1) JP5332611B2 (en)
WO (1) WO2008087895A1 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012100581A (en) * 2010-11-10 2012-05-31 Denka Seiken Co Ltd Method for quantification of remnant-like lipoprotein cholesterol and kit for the same
JP2014030393A (en) * 2012-08-03 2014-02-20 Denka Seiken Co Ltd Quantitation method of cholesterol in high density lipoprotein (hdl)
CN105137098A (en) * 2015-08-24 2015-12-09 巩晓东 Determination reagent of cholesterols in serum high density lipoprotein
JP2016082880A (en) * 2014-10-23 2016-05-19 東洋紡株式会社 Measuring method of high density lipoprotein cholesterol
JP2017060522A (en) * 2016-12-20 2017-03-30 デンカ生研株式会社 Method for quantifying cholesterol in high density lipoprotein (HDL)
WO2017204230A1 (en) * 2016-05-24 2017-11-30 デンカ生研株式会社 Method and reagent for quantifying cholesterol in triglyceride-rich lipoprotein
CN110736846A (en) * 2018-07-20 2020-01-31 上海练佰生物技术中心 Reagent, method and kit for measuring small and dense lipoproteins
CN114134202A (en) * 2021-11-26 2022-03-04 深圳市雷诺华科技实业有限公司 Method for measuring high-density lipoprotein cholesterol by using inorganic hybrid nanoflower

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6269999A (en) * 1985-09-18 1987-03-31 ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Method and reagent for specific measurement of hdl-cholestrine in serum or plasma
JPH09299A (en) * 1995-06-21 1997-01-07 Internatl Reagents Corp Determination of cholesterol in lipoprotein fraction having high specific gravity and determination reagent kit
WO1998026090A1 (en) * 1996-12-09 1998-06-18 Denka Seiken Co., Ltd. Method of determining cholesterol content of high-density lipoproteins

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3636851A1 (en) * 1986-10-29 1988-05-11 Boehringer Mannheim Gmbh METHOD AND REAGENT FOR SPECIFIC DETERMINATION OF THE CHOLESTERIN OF THE HDL FACTION
JP2653755B2 (en) * 1994-03-08 1997-09-17 協和メデックス株式会社 Determination of cholesterol in high density lipoprotein

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6269999A (en) * 1985-09-18 1987-03-31 ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Method and reagent for specific measurement of hdl-cholestrine in serum or plasma
JPH09299A (en) * 1995-06-21 1997-01-07 Internatl Reagents Corp Determination of cholesterol in lipoprotein fraction having high specific gravity and determination reagent kit
WO1998026090A1 (en) * 1996-12-09 1998-06-18 Denka Seiken Co., Ltd. Method of determining cholesterol content of high-density lipoproteins

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012100581A (en) * 2010-11-10 2012-05-31 Denka Seiken Co Ltd Method for quantification of remnant-like lipoprotein cholesterol and kit for the same
EP2639310A4 (en) * 2010-11-10 2014-04-02 Denka Seiken Kk METHOD FOR QUANTIFICATION OF RESIDUAL TYPE LIPOPROTEIN CHOLESTEROL AND CORRESPONDING KIT
JP2014030393A (en) * 2012-08-03 2014-02-20 Denka Seiken Co Ltd Quantitation method of cholesterol in high density lipoprotein (hdl)
JP2016082880A (en) * 2014-10-23 2016-05-19 東洋紡株式会社 Measuring method of high density lipoprotein cholesterol
CN105137098A (en) * 2015-08-24 2015-12-09 巩晓东 Determination reagent of cholesterols in serum high density lipoprotein
WO2017204230A1 (en) * 2016-05-24 2017-11-30 デンカ生研株式会社 Method and reagent for quantifying cholesterol in triglyceride-rich lipoprotein
JP2017060522A (en) * 2016-12-20 2017-03-30 デンカ生研株式会社 Method for quantifying cholesterol in high density lipoprotein (HDL)
CN110736846A (en) * 2018-07-20 2020-01-31 上海练佰生物技术中心 Reagent, method and kit for measuring small and dense lipoproteins
CN110736846B (en) * 2018-07-20 2023-07-14 上海微鸿企业管理有限公司 Small and dense lipoprotein determination reagent, method and kit
CN114134202A (en) * 2021-11-26 2022-03-04 深圳市雷诺华科技实业有限公司 Method for measuring high-density lipoprotein cholesterol by using inorganic hybrid nanoflower

Also Published As

Publication number Publication date
JPWO2008087895A1 (en) 2010-05-06
JP5332611B2 (en) 2013-11-06

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