[go: up one dir, main page]

WO2008084566A1 - Model mouse, method of constructing the same and use thereof - Google Patents

Model mouse, method of constructing the same and use thereof Download PDF

Info

Publication number
WO2008084566A1
WO2008084566A1 PCT/JP2007/063756 JP2007063756W WO2008084566A1 WO 2008084566 A1 WO2008084566 A1 WO 2008084566A1 JP 2007063756 W JP2007063756 W JP 2007063756W WO 2008084566 A1 WO2008084566 A1 WO 2008084566A1
Authority
WO
WIPO (PCT)
Prior art keywords
gsdmd
mouse
cells
gene
model mouse
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2007/063756
Other languages
French (fr)
Japanese (ja)
Inventor
Toshihiko Shiroishi
Masaru Tamura
Tomoaki Fujii
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Research Organization of Information and Systems
Original Assignee
Research Organization of Information and Systems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Research Organization of Information and Systems filed Critical Research Organization of Information and Systems
Priority to JP2008552997A priority Critical patent/JPWO2008084566A1/en
Publication of WO2008084566A1 publication Critical patent/WO2008084566A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

It is intended to provide a model mouse which is to be used for clarifying the functions of Gsdmd on intestinal epithelial cells and Gsdmd gene encoding the same. A mouse having a homozygote in which Gsdmd gene encoding Gsdmd has been knock-out on chromosome and not expressing Gsdmd is referred to as the model mouse. First, a targeting vector having a fragment which contains the exon 2 of Gsdmd gene in mouse genomic DNA is transferred into mouse embryonic stem cells and chimeric embryonic cells are constructed from the embryonic stem cells having the Gsdmd gene knock-out by homologous recombination. Next, these chimeric cells are transplanted into a host mouse. The Gsdmd gene-knock out chimeric mice thus bred are mated and heterozygous mice are selected. By mating these heterozygous mice with each other, a model mouse of the Gsdmd gene-knockout homozygote can be obtained.
PCT/JP2007/063756 2007-01-11 2007-07-10 Model mouse, method of constructing the same and use thereof Ceased WO2008084566A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008552997A JPWO2008084566A1 (en) 2007-01-11 2007-07-10 Model mouse, manufacturing method thereof and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-003398 2007-01-11
JP2007003398 2007-01-11

Publications (1)

Publication Number Publication Date
WO2008084566A1 true WO2008084566A1 (en) 2008-07-17

Family

ID=39608467

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/063756 Ceased WO2008084566A1 (en) 2007-01-11 2007-07-10 Model mouse, method of constructing the same and use thereof

Country Status (2)

Country Link
JP (1) JPWO2008084566A1 (en)
WO (1) WO2008084566A1 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018214823A1 (en) * 2017-05-25 2018-11-29 朱献军 METHOD FOR CONSTRUCTING MOUSE MODEL WITH CONDITIONAL KNOCKOUT OF TMEM30A GENE FROM PANCREATIC β CELL AND USE
CN114657214A (en) * 2022-02-25 2022-06-24 陕西中医药大学 Construction method of spontaneous kidney-yin deficiency Ptgds gene knockout rat model
CN115725687A (en) * 2022-10-08 2023-03-03 浙江大学 Screening Method of Porcine Antibacterial Short Peptides
CN116369278A (en) * 2023-05-17 2023-07-04 上海溯源生物技术有限公司 Animal model construction method for helicobacter pylori infection

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004076666A1 (en) * 2003-02-28 2004-09-10 Toshihiko Shiroishi Gasdermin family
WO2006019153A1 (en) * 2004-08-20 2006-02-23 Inter University Research Institution Corporation, Research Organization Of Information And Systems NONHUMAN ANIMAL WITH INHIBITED GdsmA GENE FUNCTION AND PROMOTED OR INHIBITIED CANCERATION-ASSOCIATED GENE FUNCTION

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004076666A1 (en) * 2003-02-28 2004-09-10 Toshihiko Shiroishi Gasdermin family
WO2006019153A1 (en) * 2004-08-20 2006-02-23 Inter University Research Institution Corporation, Research Organization Of Information And Systems NONHUMAN ANIMAL WITH INHIBITED GdsmA GENE FUNCTION AND PROMOTED OR INHIBITIED CANCERATION-ASSOCIATED GENE FUNCTION

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
FUJII T. ET AL.: "Expression and functional analyses of GasderminD(GsdmD)", GENES & GENETIC SYSTEMS, vol. 80, no. 6, 2005, pages 462, XP003022943 *
FUJII T. ET AL.: "Gasdermin (Gsdm) is involved in maintaining of homeostasis of intestinal epithelial ceels in intestinal tract", GENES & GENETIC SYSTEMS, vol. 81, no. 6, December 2006 (2006-12-01), pages 450, XP003022935 *
FUJII T. ET AL.: "Gasdermin(Gsdm)family Idenshi no Tanri to sono Hatsugen Kaiseki", JAPAN SOCIETY OF DEVELOPMENT BIOLOGISTS TAIKAI HAPPYO YOSHISHU, vol. 36TH, 2003, pages 55, XP002996880 *
FUJII T. ET AL.: "Shokakan ni Oite, Gasdermin (Gsdm) wa Johi Saibo no Kojosei Iji ni Kanyo suru", 78TH ANNUAL MEETING OF THE GENETICS SOECIETY OF JAPAN, PROGRAMS & ABSTRACTS, 1 September 2006 (2006-09-01), pages 164, 2E08, XP003022936 *
FUJII T. ET AL.: "Shokakan no Oite, Gasdermin (Gsdm) wa Johi Saibo no Kojosei Iji ni Kanyo suru", THE GENETICS SOCIETY OF JAPAN, 26 September 2006 (2006-09-26), XP003022942, Retrieved from the Internet <URL:http://www.brc.riken.jp/iden78/program2.pdf> *
FUJII T.: "Expression profiling and functional analysis of Gasdermin (Gsdm) family genes in mouse intestinal tract", 28 August 2006 (2006-08-28), XP003022937, Retrieved from the Internet <URL:http://www.nig.ac.jp/cgi-bin/seminar.pl?action=display&target=20060828005_&lang=Japanese> *
KATOH M. ET AL.: "Identification and characterization of human DFNA5L, mouse Dfna5l, and rat Dfna5l genes in silico", INTERNATIONAL JOURNAL OF ONCOLOGY, vol. 25, no. 3, 2004, pages 765 - 770, XP003022938 *
SUN Q. ET AL.: "Progress of researches on gene function of GSDMDC family", YI CHUAN, vol. 28, no. 5, May 2006 (2006-05-01), pages 596 - 600, XP003022939 *
TAMURA M. ET AL.: "Gasdermin(Gsdm/GSDM) family no Hatsugen to Shinkateki Sokumen ni Tsuite", JAPAN SOCIETY OF DEVELOPMENT BIOLOGISTS TAIKAI HAPPYO YOSHISHU, vol. 39TH, 2006, pages 174, XP003022940 *
TAMURA M. ET AL.: "Shinki Gsdm family Isenshi no Tanri to sono Hatsugen Kaiseki", JAPAN SOCIETY OF DEVELOPMENT BIOLOGISTS TAIKAI HAPPYO YOSHISHU, vol. 38TH, 2005, pages 243, XP003022941 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018214823A1 (en) * 2017-05-25 2018-11-29 朱献军 METHOD FOR CONSTRUCTING MOUSE MODEL WITH CONDITIONAL KNOCKOUT OF TMEM30A GENE FROM PANCREATIC β CELL AND USE
CN114657214A (en) * 2022-02-25 2022-06-24 陕西中医药大学 Construction method of spontaneous kidney-yin deficiency Ptgds gene knockout rat model
CN114657214B (en) * 2022-02-25 2024-04-16 陕西中医药大学 Construction method of spontaneous kidney-yin deficiency Ptgds gene knockout rat model
CN115725687A (en) * 2022-10-08 2023-03-03 浙江大学 Screening Method of Porcine Antibacterial Short Peptides
CN115725687B (en) * 2022-10-08 2023-12-12 浙江大学 Screening method for pig-derived antibacterial short peptides
CN116369278A (en) * 2023-05-17 2023-07-04 上海溯源生物技术有限公司 Animal model construction method for helicobacter pylori infection

Also Published As

Publication number Publication date
JPWO2008084566A1 (en) 2010-04-30

Similar Documents

Publication Publication Date Title
Ren et al. The subgenomes show asymmetric expression of alleles in hybrid lineages of Megalobrama amblycephala× Culter alburnus
Yen et al. Somatic mosaicism and allele complexity induced by CRISPR/Cas9 RNA injections in mouse zygotes
Hruscha et al. Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish
Yoshimi et al. Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR–Cas platform
CN108192981B (en) The white red plumage reason mutated-genotype identification of Leghorn and red feather pink-egg layer chickens breed system breeding method
Kanki et al. High-efficiency CAG-FLPe deleter mice in C57BL/6J background
Oliver et al. Pervasive genotypic mosaicism in founder mice derived from genome editing through pronuclear injection
Nunn et al. Chromosome‐level Thlaspi arvense genome provides new tools for translational research and for a newly domesticated cash cover crop of the cooler climates
UA103887C2 (en) Method for production of herbicide resistant plant
Fang et al. Generation of albino medaka (Oryzias latipes) by CRISPR/Cas9
WO2019103442A3 (en) Genome editing composition using crispr/cpf1 system and use thereof
JPWO2016104716A1 (en) Gene knockout method
WO2015052231A3 (en) Multiplex editing system
TW201217533A (en) Genomics of actinoplanes utahensis
JP2010500029A5 (en)
WO2007130804A3 (en) Method for producing fertile crosses between wild and domestic soybean species
WO2011101811A3 (en) Improved meganuclease recombination system
WO2009045550A3 (en) System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts
WO2008084566A1 (en) Model mouse, method of constructing the same and use thereof
Yin et al. DNA repair-and nucleotide metabolism-related genes exhibit differential CHG methylation patterns in natural and synthetic polyploids (Brassica napus L.)
CN112980846A (en) Construction method of Pax2 conditional gene knockout mouse model
WO2007126850A3 (en) Photoperiodic control of floret differentiation and yield in plants
Markossian et al. CRISPR/Cas9: a breakthrough in generating mouse models for endocrinologists
Wang et al. Improving the genome editing efficiency of CRISPR/Cas9 in melon and watermelon
MX2012003816A (en) Dna construct, and process for production of recombinant cho cell using same.

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07790574

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2008552997

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07790574

Country of ref document: EP

Kind code of ref document: A1