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WO2008061311A3 - Hybridation perfectionnée d'acides nucléiques - Google Patents

Hybridation perfectionnée d'acides nucléiques Download PDF

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Publication number
WO2008061311A3
WO2008061311A3 PCT/AU2007/001797 AU2007001797W WO2008061311A3 WO 2008061311 A3 WO2008061311 A3 WO 2008061311A3 AU 2007001797 W AU2007001797 W AU 2007001797W WO 2008061311 A3 WO2008061311 A3 WO 2008061311A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
probe
oligonucleotide
hybridisation
oligonucleotide probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/AU2007/001797
Other languages
English (en)
Other versions
WO2008061311A2 (fr
Inventor
Peter Laurence Molloy
Maxine June Mccall
Horace R Drew
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commonwealth Scientific and Industrial Research Organization CSIRO
Original Assignee
Commonwealth Scientific and Industrial Research Organization CSIRO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2006906520A external-priority patent/AU2006906520A0/en
Application filed by Commonwealth Scientific and Industrial Research Organization CSIRO filed Critical Commonwealth Scientific and Industrial Research Organization CSIRO
Priority to AU2007324273A priority Critical patent/AU2007324273A1/en
Priority to EP07815599A priority patent/EP2087133A4/fr
Publication of WO2008061311A2 publication Critical patent/WO2008061311A2/fr
Publication of WO2008061311A3 publication Critical patent/WO2008061311A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention porte sur un procédé destiné à réduire le mauvais appariement de nucléotides dans une hybridation entre une sonde oligonucléotidique et un ADN cible, le procédé comprenant les opérations consistant à prendre au moins une sonde oligonucléotidique d'environ 7 à environ 25 nucléotides, à prendre un échantillon comprenant de l'ADN cible, dans lequel l'ADN est préparé de manière à comprendre des fragments allant jusqu'à environ 100 bases, faire incuber l'ADN avec la au moins une sonde oligonucléotidique dans des conditions appropriées pour permettre une hybridation entre la sonde et l'ADN cible, éliminer l'ADN non lié et détecter l'ADN hybridé à la au moins une sonde oligonucléotidique, procédé suivant lequel l'un ou l'autre de l'ADN et de la au moins une sonde oligonucléotidique, ou les deux comprennent un ou plusieurs nucléotides modifiés, et suivant lequel l'ADN de l'échantillon est facultativement marqué par une fraction détectable.
PCT/AU2007/001797 2006-11-22 2007-11-22 Hybridation perfectionnée d'acides nucléiques Ceased WO2008061311A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2007324273A AU2007324273A1 (en) 2006-11-22 2007-11-22 Improved hybridisation of nucleic acids
EP07815599A EP2087133A4 (fr) 2006-11-22 2007-11-22 Hybridation perfectionnée d'acides nucléiques

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2006906520 2006-11-22
AU2006906520A AU2006906520A0 (en) 2006-11-22 Improved hybridisation of nucleic acids

Publications (2)

Publication Number Publication Date
WO2008061311A2 WO2008061311A2 (fr) 2008-05-29
WO2008061311A3 true WO2008061311A3 (fr) 2008-10-02

Family

ID=39430076

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2007/001797 Ceased WO2008061311A2 (fr) 2006-11-22 2007-11-22 Hybridation perfectionnée d'acides nucléiques

Country Status (3)

Country Link
EP (1) EP2087133A4 (fr)
AU (1) AU2007324273A1 (fr)
WO (1) WO2008061311A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9879222B2 (en) 2007-12-14 2018-01-30 Mofa Group Llc Gender-specific separation of sperm cells and embryos
WO2011032034A2 (fr) 2009-09-10 2011-03-17 University Of Idaho Nucléotides et oligonucléotides à conformation restreinte à fonctionnalité nucléobase pour cibler des acides nucléiques
MX2014000797A (es) 2011-07-19 2014-07-09 Univ Idaho Sonda y metodo para direccionar acidos nucleicos.
US9365902B2 (en) * 2013-03-15 2016-06-14 Abbott Molecular Inc. Detection of bisulfite converted nucleotide sequences
US20190316112A1 (en) * 2018-04-12 2019-10-17 Cellmax, Ltd. Methods of capturing a nucleic acid including a target oligonucleotide sequence and uses thereof
US20190316195A1 (en) * 2018-04-12 2019-10-17 Cellmax, Ltd. Methods of capturing a nucleic acid including a target oligonucleotide sequence and uses thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001064958A2 (fr) * 2000-03-01 2001-09-07 Epoch Bioscienecs, Inc. Oligonucleotides modifies permettant de discriminer des mesappariements
WO2002038801A1 (fr) * 2000-11-13 2002-05-16 Human Genetic Signatures Pty Ltd Detection de molecules d'adn methylees
EP1247815A2 (fr) * 2001-03-25 2002-10-09 Exiqon A/S Oligonucléotides modifiés et leurs utilisations
WO2006097234A2 (fr) * 2005-03-18 2006-09-21 Eppendorf Ag Jeu ordonne d'echantillons et methode servant a genotyper des shv beta lactamases

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1838870A2 (fr) * 2004-12-29 2007-10-03 Exiqon A/S Nouvelles compositions d'oligonucleotides et sequences de sondes utiles pour la detection et l'analyse de microarn et de leurs marn cibles

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001064958A2 (fr) * 2000-03-01 2001-09-07 Epoch Bioscienecs, Inc. Oligonucleotides modifies permettant de discriminer des mesappariements
WO2002038801A1 (fr) * 2000-11-13 2002-05-16 Human Genetic Signatures Pty Ltd Detection de molecules d'adn methylees
EP1247815A2 (fr) * 2001-03-25 2002-10-09 Exiqon A/S Oligonucléotides modifiés et leurs utilisations
WO2006097234A2 (fr) * 2005-03-18 2006-09-21 Eppendorf Ag Jeu ordonne d'echantillons et methode servant a genotyper des shv beta lactamases

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
CHEN D. ET AL.: "Analysis of internal (eta-1)mer deletion sequences in synthetic oligodeoxyribonucleotides by hybridization to an immobilized probe array", NUCLEIC ACIDS RESEARCH, vol. 27, no. 2, 1999, pages 389 - 395, XP009081878 *
LANE S. ET AL.: "Amplicon secondary structure prevents target hybridization to oligonucleotide microarrays", BIOSENSORS AND BIOELECTRONICS, vol. 20, 2004, pages 728 - 735, XP004633820 *
LOCKHARDT D.J. ET AL.: "Expression monitoring by hybridization to high-density oligoncleotide arrays", NATURE BIOTECHNOLOGY, vol. 14, 1996, pages 1675 - 1680, XP002022521 *
MAH N. ET AL.: "A comparison of oligonucleotide and cDNA-based microarray systems", PHYSIOLOGICAL GENOMICS, vol. 16, 2004, pages 361 - 370, XP008109647 *
MEHLMANN M. ET AL.: "Optimization of fragmentation conditions for microarrays analysis of viral RNA", ANALYTICAL BIOCHEMISTRY, vol. 347, 2005, pages 316 - 323, XP005176365 *
RELOGIO A. ET AL.: "Optimization of oligonucleotide-based DNA microarrays", NUCLEIC ACIDS RESEARCH, vol. 30, no. 11, 2002, pages E51-1 - E51-10, XP002316925 *
RICELLI P.V. ET AL.: "Hybridization of single-stranded DNA targets to immobilized complementary DNA probes: comparison of hairpin versus linear capture probes", NUCLEIC ACIDS RESEARCH, vol. 29, no. 4, 2001, pages 996 - 1004, XP002282478 *
ROSENOW C. ET AL.: "Proparyotic RNA preparation methods useful for high density array analysis: comparison of two approaches", NUCLEIC ACIDS RESEARCH, vol. 29, no. 22, 2001, pages E112-1 - E112-8, XP002380280 *
See also references of EP2087133A4 *
SEELA F. ET AL.: "Pyrazolo[3,4,-d]pyrimidine nucleic acids: adjustment of dA-dT to dG-dC base pair stability", NUCLEIC ACIDS RESEARCH, vol. 29, no. 10, 2001, pages 2069 - 2078, XP008109646 *
SOUTHERN E. ET AL.: "Molecular interactions on microarrays", NATURE GENETICS, vol. 21, no. SUPPL., 1999, pages 5 - 9, XP000865979 *
YOU Y. ET AL.: "Design of LNA probes that improve mismatch discrimination", NUCLEIC ACIDS RESEARCH, vol. 34, no. 8, May 2006 (2006-05-01), pages E60-1 - E60-11, XP002498572 *

Also Published As

Publication number Publication date
EP2087133A4 (fr) 2010-07-21
AU2007324273A1 (en) 2008-05-29
WO2008061311A2 (fr) 2008-05-29
EP2087133A2 (fr) 2009-08-12

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