[go: up one dir, main page]

WO2008060449A3 - Détecteur microfluidique - Google Patents

Détecteur microfluidique Download PDF

Info

Publication number
WO2008060449A3
WO2008060449A3 PCT/US2007/023595 US2007023595W WO2008060449A3 WO 2008060449 A3 WO2008060449 A3 WO 2008060449A3 US 2007023595 W US2007023595 W US 2007023595W WO 2008060449 A3 WO2008060449 A3 WO 2008060449A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
articles
captured
determining
fluid sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/023595
Other languages
English (en)
Other versions
WO2008060449A2 (fr
Inventor
Manish J Butte
Amy P Wong
Arlene H Sharpe
George M Whitesides
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of WO2008060449A2 publication Critical patent/WO2008060449A2/fr
Publication of WO2008060449A3 publication Critical patent/WO2008060449A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/24—Immunology or allergic disorders

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des articles et des procédés permettant de déterminer un analyte indiquant une condition de maladie. Dans certains modes de réalisation, les articles et procédés décrits ici peuvent être utilisés pour déterminer, qualitativement ou quantitativement, une présence d'un composant, tel qu'un type de cellule particulier, dans un échantillon de fluide. Dans un mode de réalisation particulier, on propose un système microfluidique à faible coût permettant une détection rapide de cellules T. Le système microfluidique peut utiliser des anticorps immobilisés et des molécules d'adhérence dans un canal pour capturer les cellules T à partir d'un échantillon de fluide tel qu'un petit volume de sang. Les cellules T capturées peuvent être étiquetées avec un colloïde métallique (par exemple des nanoparticules d'or) en utilisant un anticorps spécifique au récepteur de cellules T (TCR), et de l'argent métallique peut être précipité de manière catalytique sur les cellules. Le nombre de cellules T capturées peut être compté et peut indiquer une condition de maladie d'un patient telle qu'une déficience immune combinée sévère ou un virus d'immunodéficience humaine.
PCT/US2007/023595 2006-11-09 2007-11-09 Détecteur microfluidique Ceased WO2008060449A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US85808406P 2006-11-09 2006-11-09
US60/858,084 2006-11-09

Publications (2)

Publication Number Publication Date
WO2008060449A2 WO2008060449A2 (fr) 2008-05-22
WO2008060449A3 true WO2008060449A3 (fr) 2008-08-07

Family

ID=39304576

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/023595 Ceased WO2008060449A2 (fr) 2006-11-09 2007-11-09 Détecteur microfluidique

Country Status (1)

Country Link
WO (1) WO2008060449A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2009267803B2 (en) 2008-07-11 2016-04-21 Monash University Method of fabricating microfluidic systems
EP2318304B1 (fr) 2008-08-14 2019-11-20 Monash University Commutateurs pour systèmes microfluidiques
WO2016112245A1 (fr) * 2015-01-08 2016-07-14 Hackensack University Medical Center Procédés ex vivo pour minimiser les risques et maximiser les effets bénéfiques d'une transfusion de sang allogénique et greffe de moelle
WO2016209775A1 (fr) * 2015-06-20 2016-12-29 The Regents Of The University Of California Dosage microfluidique basé sur des intégrines de monocytes destiné à l'évaluation de maladies coronaires

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040248205A1 (en) * 2003-04-16 2004-12-09 Stern Lawrence J. Major histocompatibility complex (MHC)-peptide arrays
US20050042623A1 (en) * 2002-10-30 2005-02-24 Dana Ault-Riche Systems for capture and analysis of biological particles and methods using the systems
WO2005066613A1 (fr) * 2003-12-31 2005-07-21 President And Fellows Of Harvard College Dispositif et procede de dosage

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050042623A1 (en) * 2002-10-30 2005-02-24 Dana Ault-Riche Systems for capture and analysis of biological particles and methods using the systems
US20040248205A1 (en) * 2003-04-16 2004-12-09 Stern Lawrence J. Major histocompatibility complex (MHC)-peptide arrays
WO2005066613A1 (fr) * 2003-12-31 2005-07-21 President And Fellows Of Harvard College Dispositif et procede de dosage

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BERG N N ET AL: "Characterization of intercellular adhesion molecule-1 (ICAM-1)-augmented degranulation by cytotoxic T cells. ICAM-1 and anti-CD3 must be co-localized for optimal adhesion and stimulation.", JOURNAL OF IMMUNOLOGY (BALTIMORE, MD. : 1950) 15 AUG 1995, vol. 155, no. 4, 15 August 1995 (1995-08-15), pages 1694 - 1702, XP002478490, ISSN: 0022-1767 *
BUTTE ET AL: "Microfluidic Device Allows Low-Cost Newborn Screening for Severe Combined Immune Deficiency (SCID)", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, MOSBY - YEARLY BOOK, INC, US, vol. 117, no. 2, February 2006 (2006-02-01), pages S325, XP005360657, ISSN: 0091-6749 *
MISHRA ET AL: "On-chip micro-biosensor for the detection of human CD4<+> cells based on AC impedance and optical analysis", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 21, no. 5, 15 November 2005 (2005-11-15), pages 696 - 704, XP005122213, ISSN: 0956-5663 *
STONE D J ET AL: "HLA-restricted epitope identification and detection of functional Tcell responses by using MHC-peptide and costimulatory microarrays", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 102, no. 10, 8 March 2005 (2005-03-08), pages 3744 - 3749, XP002383140, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
WO2008060449A2 (fr) 2008-05-22

Similar Documents

Publication Publication Date Title
Funari et al. Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip
Pittman et al. Saliva-based microfluidic point-of-care diagnostic
Mustafa et al. Molecularly imprinted polymers in diagnostics: accessing analytes in biofluids
Raziq et al. Development of a portable MIP-based electrochemical sensor for detection of SARS-CoV-2 antigen
Babu et al. Conventional and nanotechnology based sensors for creatinine (A kidney biomarker) detection: A consolidated review
Tang et al. High-throughput electrochemical microfluidic immunoarray for multiplexed detection of cancer biomarker proteins
Qureshi et al. Biosensors for detecting viral and bacterial infections using host biomarkers: a review
Lee et al. Recent advances in lab-on-paper diagnostic devices using blood samples
US9746441B2 (en) Sensor, sensor kit and method for detecting an analyte
JP2015523054A5 (fr)
JP2017538917A (ja) Ｌｓｐｒセンサを取り込むモバイル／装着式デバイス
CN103874924A (zh) 通过一次的试样注入就能够依次改变反应条件的膜传感器
WO2007046825A3 (fr) Systeme de detection optoelectronique
EP2194380A3 (fr) Détection d&#39;un antigène- cible, indépendamment de la présence ou absence d&#39;anticorps thérapeutiques correspondants
WO2007005666A3 (fr) Systeme et methode de detection d&#39;analytes au moyen de recepteurs differentiels
WO2008060449A3 (fr) Détecteur microfluidique
Breault-Turcot et al. Microdialysis SPR: diffusion-gated sensing in blood
Daurai et al. Point-of-care biosensors for healthcare applications
Huang et al. A two-step competition assay for visual, sensitive and quantitative C-reactive protein detection in low-cost microfluidic particle accumulators
Ning et al. Evaluation of SARS-CoV-2-specific T-cell activation with a rapid on-chip IGRA
Ng et al. Label-free impedance detection of low levels of circulating endothelial progenitor cells for point-of-care diagnosis
Tsounidi et al. Current progress on biosensors and Point-of-Care devices for sepsis diagnosis
WO2018199840A1 (fr) Procédé et système microfluidiques pour la détection de la sensibilité seuil d&#39;allergènes basophiles (cd-sens) à l&#39;aide d&#39;un gradient d&#39;au moins un allergène
Xia et al. Application and research development of surface plasmon resonance-based immunosensors for protein detection
Heo et al. Simple diagnosis of HbA1c using the dual-plasmonic platform integrated with LSPR and SERS

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07867394

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07867394

Country of ref document: EP

Kind code of ref document: A2