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WO2007137700A3 - Mutierte dna-polymerasen mit höherer selektivität - Google Patents

Mutierte dna-polymerasen mit höherer selektivität Download PDF

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Publication number
WO2007137700A3
WO2007137700A3 PCT/EP2007/004315 EP2007004315W WO2007137700A3 WO 2007137700 A3 WO2007137700 A3 WO 2007137700A3 EP 2007004315 W EP2007004315 W EP 2007004315W WO 2007137700 A3 WO2007137700 A3 WO 2007137700A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
polymerase
polymerases
higher selectivity
mutated dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2007/004315
Other languages
English (en)
French (fr)
Other versions
WO2007137700A2 (de
Inventor
Andreas Marx
Nicolas Rudinger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitaet Konstanz
Original Assignee
Universitaet Konstanz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitaet Konstanz filed Critical Universitaet Konstanz
Publication of WO2007137700A2 publication Critical patent/WO2007137700A2/de
Publication of WO2007137700A3 publication Critical patent/WO2007137700A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft eine DNA-Polymerase mit erhöhter Fehlpaarungsdiskriminierung, welche im Vergleich zur Wildtyp-Variante mindestens eine Mutation in ihrer Aminosäure-Sequenz aufweist, sowie eine Wirtszelle und ein Verfahren zur Herstellung der DNA-Polymerase. Desweiteren betrifft die vorliegende Erfindung die Verwendung der DNA-Polymerase in diagnostischen und molekularbiologischen Verfahren und ein Kit, welches die DNA-Polymerase enthält.
PCT/EP2007/004315 2006-05-30 2007-05-15 Mutierte dna-polymerasen mit höherer selektivität Ceased WO2007137700A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102006025153.9 2006-05-30
DE200610025153 DE102006025153A1 (de) 2006-05-30 2006-05-30 Mutierte DNA-Polymerasen mit höherer Selektivität

Publications (2)

Publication Number Publication Date
WO2007137700A2 WO2007137700A2 (de) 2007-12-06
WO2007137700A3 true WO2007137700A3 (de) 2008-03-20

Family

ID=38650307

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/004315 Ceased WO2007137700A2 (de) 2006-05-30 2007-05-15 Mutierte dna-polymerasen mit höherer selektivität

Country Status (2)

Country Link
DE (1) DE102006025153A1 (de)
WO (1) WO2007137700A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LU92320B1 (en) 2013-12-02 2015-06-03 Univ Konstanz Mutated DNA polymerases with high selectivity and activity
CN117821413B (zh) * 2023-12-15 2024-12-27 湖北大学 一种高进行性Pfu DNA聚合酶及其制备方法和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005074350A2 (de) * 2004-02-05 2005-08-18 Rheinische Friedrich-Wilhelms- Universität Bonn Mutierte dna-polymerasen mit erhöhter fehlpaarungs-diskriminierung

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030180741A1 (en) * 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005074350A2 (de) * 2004-02-05 2005-08-18 Rheinische Friedrich-Wilhelms- Universität Bonn Mutierte dna-polymerasen mit erhöhter fehlpaarungs-diskriminierung

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BRAITHWAITE DAN K ET AL: "Compilation, alignment, and phylogenetic relationships of DNA polymerases", NUCLEIC ACIDS RESEARCH, IRL PRESS LTD., OXFORD, GB, vol. 21, no. 4, 1993, pages 787 - 802, XP002143036, ISSN: 0305-1048 *
DELARUE M ET AL: "AN ATTEMPT TO UNIFY THE STRUCTURE OF POLYMERASES", PROTEIN ENGINEERING, vol. 3, no. 6, 1990, pages 461 - 468, XP009093593, ISSN: 0269-2139 *
MINNICK T ET AL: "side chains that influence fidelity at the polymerase active site of Escherichia coli DNA polymerase I (kleenow fragment)", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 274, no. 5, 29 January 1999 (1999-01-29), pages 3067 - 3075, XP002341285, ISSN: 0021-9258 *
STRERATH MICHAEL ET AL: "Directed DNA polymerase evolution: Effects of mutations in motif C on the mismatch-extension selectivity of Thermus aquaticus DNA polymerase", CHEMBIOCHEM, vol. 8, no. 4, March 2007 (2007-03-01), pages 395 - 401, XP009093564, ISSN: 1439-4227 *
SUMMERER,D. ET AL., ANGEW. CHEM. INT. ED., vol. 44, 2005, pages 4712 - 4715, XP002462227 *

Also Published As

Publication number Publication date
WO2007137700A2 (de) 2007-12-06
DE102006025153A1 (de) 2007-12-06

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