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WO2007132164A3 - Analysis of proteins - Google Patents

Analysis of proteins Download PDF

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Publication number
WO2007132164A3
WO2007132164A3 PCT/GB2007/001621 GB2007001621W WO2007132164A3 WO 2007132164 A3 WO2007132164 A3 WO 2007132164A3 GB 2007001621 W GB2007001621 W GB 2007001621W WO 2007132164 A3 WO2007132164 A3 WO 2007132164A3
Authority
WO
WIPO (PCT)
Prior art keywords
product
protein extract
proteins
analysis
modified plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2007/001621
Other languages
French (fr)
Other versions
WO2007132164A2 (en
Inventor
Peter Bramley
Paul Fraser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Royal Holloway and Bedford New College
Original Assignee
Royal Holloway and Bedford New College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0608602A external-priority patent/GB0608602D0/en
Priority claimed from GB0608604A external-priority patent/GB0608604D0/en
Application filed by Royal Holloway and Bedford New College filed Critical Royal Holloway and Bedford New College
Publication of WO2007132164A2 publication Critical patent/WO2007132164A2/en
Publication of WO2007132164A3 publication Critical patent/WO2007132164A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for determining the presence of genetically-modified plant- derived material in a product, the method comprising: providing a protein extract derived from the product; enriching the protein extract; digesting the protein extract using an enzyme; and detecting the presence or absence of at least one marker peptide resulting from the enzymatic digestion of a transgenic protein thereby determining whether the genetically modified plant derived material is present in the product.
PCT/GB2007/001621 2006-05-02 2007-05-02 Analysis of proteins Ceased WO2007132164A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0608602A GB0608602D0 (en) 2006-05-02 2006-05-02 Analysis of proteins
GB0608602.9 2006-05-02
GB0608604A GB0608604D0 (en) 2006-05-02 2006-05-02 Mass spectrometric analysis of proteins
GB0608604.5 2006-05-02

Publications (2)

Publication Number Publication Date
WO2007132164A2 WO2007132164A2 (en) 2007-11-22
WO2007132164A3 true WO2007132164A3 (en) 2008-03-27

Family

ID=38441488

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/001621 Ceased WO2007132164A2 (en) 2006-05-02 2007-05-02 Analysis of proteins

Country Status (1)

Country Link
WO (1) WO2007132164A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA112515C2 (en) * 2009-06-03 2016-09-26 Дау Аґросаєнсиз Елелсі METHOD OF DETERMINING THE PRESENCE OF TWO OR MORE PROTEINS OF INTEREST, IN SAMPLES OF VEGETABLE ORIGIN
JPWO2014010700A1 (en) * 2012-07-12 2016-06-23 国立大学法人九州大学 Amino compound and its sensitive mass spectrometry method and biomarker assay method
CN103698418B (en) * 2013-11-12 2015-07-08 北京理工大学 Quantitative detection method for transgene protein CP4-EPSPS in plant
DE102014008135A1 (en) * 2014-06-06 2015-12-17 Gfl Gesellschaft Für Lebensmittel-Forschung Mbh Method for analytical authenticity testing of ingredients in food using artificial antibodies
EP3155432A1 (en) * 2014-06-10 2017-04-19 Dow AgroSciences LLC Quantitative analysis of transgenic proteins
CN106841458A (en) * 2017-03-17 2017-06-13 中国肉类食品综合研究中心 The method that Liquid Chromatography-Tandem Mass Spectrometry determines plant derived component in meat product
CN109521105A (en) * 2018-08-03 2019-03-26 西北工业大学 A kind of identification method for the wheaten food historical relic that is unearthed

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001098523A2 (en) * 2000-06-20 2001-12-27 Gd Biotech Co., Ltd Detection method of genetic recombinant food and detection kit of that
WO2002084250A2 (en) * 2001-04-17 2002-10-24 Femtolink Biotechnologies Llc Methods for mass spectrometry detection and quantification of specific target proteins in complex biological samples

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001098523A2 (en) * 2000-06-20 2001-12-27 Gd Biotech Co., Ltd Detection method of genetic recombinant food and detection kit of that
WO2002084250A2 (en) * 2001-04-17 2002-10-24 Femtolink Biotechnologies Llc Methods for mass spectrometry detection and quantification of specific target proteins in complex biological samples

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KREKEL F ET AL: "SUBSTRATE AND INHIBITOR-INDUCED CONFORMATIONAL CHANGES IN THE STRUCTURALLY RELATED ENZYMES UDP-N-ACETYLGLUCOSAMINE ENOPYRUVYL TRANSFERASE (MURA) AND 5-ENOLPYRUVYLSHIKIMATE 3-PHOSPHATE SYNTHASE (EPSP)", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 38, no. 28, 1999, pages 8864 - 8878, XP002931371, ISSN: 0006-2960 *
OCANA MIREIA FERNANDEZ ET AL: "Mass spectrometric detection of CP4 EPSPS in genetically modified soya and maize", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 21, no. 3, 2007, pages 319 - 328, XP002451539, ISSN: 0951-4198 *

Also Published As

Publication number Publication date
WO2007132164A2 (en) 2007-11-22

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