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WO2007126191A1 - Procédé de préparation de choline à protection contre la destruction ruminale - Google Patents

Procédé de préparation de choline à protection contre la destruction ruminale Download PDF

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Publication number
WO2007126191A1
WO2007126191A1 PCT/KR2006/004773 KR2006004773W WO2007126191A1 WO 2007126191 A1 WO2007126191 A1 WO 2007126191A1 KR 2006004773 W KR2006004773 W KR 2006004773W WO 2007126191 A1 WO2007126191 A1 WO 2007126191A1
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WO
WIPO (PCT)
Prior art keywords
choline
coated
preparing
encapsulated
ruminally protected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2006/004773
Other languages
English (en)
Inventor
Wang Shik Lee
Hyeon Seop Kim
Hyun June Lee
Kwang Seok Ki
Soo Bong Park
Hyun Soo Kim
Sang Hoon Bong
Jeon Soo Hong
Min Kyo Chung
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nuvo B & T Corp
RURAL DEVELOPMENT ADMINISTRATION NATIONAL LIVESTOCK RESEARCH INSTITUTE
Original Assignee
Nuvo B & T Corp
RURAL DEVELOPMENT ADMINISTRATION NATIONAL LIVESTOCK RESEARCH INSTITUTE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nuvo B & T Corp, RURAL DEVELOPMENT ADMINISTRATION NATIONAL LIVESTOCK RESEARCH INSTITUTE filed Critical Nuvo B & T Corp
Priority to CN2006800544697A priority Critical patent/CN101431903B/zh
Priority to JP2009509394A priority patent/JP5159766B2/ja
Publication of WO2007126191A1 publication Critical patent/WO2007126191A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
    • A23K40/35—Making capsules specially adapted for ruminants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/02—Nutrients, e.g. vitamins, minerals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

  • the present invention relates to a method for preparing ruminally protected choline, which is not degraded by microorganisms, pH and water in the rumen, and thus can be stably delivered and absorbed in the lower digestive tract. More particularly, the present invention relates to a method for preparing double-coated ruminally protected choline, comprising the steps of: (a) preparing carrier- immobilized choline by immobilizing concentrated liquid choline on a carrier and then removing water from the immobilized choline; (b) preparing a first encapsulated choline by encapsulating the carrier-immobilized choline with a binder/coating material; (c) screening encapsulated choline having a size of 0.5-2.0 mm from the first encapsulated choline; and (d) further encapsulating the screened encapsulated choline with any one or a combination of two or more selected from the group consisting of extremely hydrogenated oils and fats, and fatty
  • Choline is known as an essential component in studies on some livestock, but the choline requirements for lactating dairy cows have not been established. Also, most choline is degraded in the rumen of ruminant animals, and thus there is a need to develop a technology of protecting choline from degradation such that choline can be passed through the rumen and absorbed and used in the small intestine.
  • Lactation response to choline is similar to the response of methionine, which is an essential amino acid, and typical symptoms of choline deficiency include muscular weakness, the occurrence of fatty liver, renal bleeding, and the like.
  • choline is a limiting nutrient in diary cows. Also, in choline deficiency, fatty liver occurred due to the fat mechanism and abnormal secretory mechanism of the liver, and when the injection of choline was increased, the migration of fatty acid was increased, thus leading to a reduction in occurrence of fatty liver.
  • choline affects blood glucose, plasma cholesterol, serum insulin and serum NEFA (nonesterified fatty acids) to significantly contribute to improvements in milk production, milk composition, weight gain and carcass characteristics, but choline chloride supplemented in feed is mostly degraded in the rumen.
  • Ruminal microorganisms degrade phosphatidyl choline in feed into choline and phosphodiglycerides and also degrade choline by converting choline to methane via trimethylamine as an intermediate metabolite. As a result, less than 1% of choline in feed is known to reach the small intestine.
  • Choline is an essential nutrient in livestock animals, but the effect thereof cannot be seen in ruminant animals because it is degraded in the rumen. For this reason, attempts to prevent choline components from being degraded in the rumen have long been made.
  • WO 94/15480 discloses feeding an encapsulated choline composition to ruminants to increase milk production.
  • a hydrophobic coating is also used and a method for preparing the choline composition is not specifically mentioned.
  • WO 96/08168 discloses a ruminant feed additive which is prepared by encapsulating a liquid choline compound in a carrier and coating the compound with fatty acid or fatty acid salt.
  • a ruminant feed additive which is prepared by encapsulating a liquid choline compound in a carrier and coating the compound with fatty acid or fatty acid salt.
  • ruminally protected choline products which are commercially available currently, there are products prepared by mixing choline with a protective substance and subjecting the mixture to a cold spray process. In this case, however, high costs of factory sites and high installation costs are required, and very cold air having a temperature of -60 °C is used, thus leading to an increase in production cost.
  • the present inventors have made extensive efforts to solve the problem that choline is degraded in the rumen of ruminants.
  • the present inventors have found that ruminally protected choline, prepared by immobilizing choline on a carrier, removing water from the immobilized choline, encapsulating the water-free choline with a hydrophobic substance, and then further encapsulating the encapsulated choline with a substance selected from among extremely hydrogenated oils and fats, and fatty acids having a melting point of more than 40 °C , is stably delivered and absorbed into the lower digestive tract without being degraded by microorganisms, pH and water in the rumen, thereby completing the present invention.
  • the present invention provides a method for preparing double-coated ruminally protected choline, the method comprises the steps of: (a) preparing carrier-immobilized choline by immobilizing concentrated liquid choline on a carrier and then removing water from the immobilized choline; (b) preparing a first encapsulated choline by encapsulating the carrier-immobilized choline with a binder/coating material; (c) screening encapsulated choline having a size of 0.5-2.0 mm from the first encapsulated choline; and (d) further encapsulating the screened encapsulated choline with any one or a combination of two or more selected from the group consisting of extremely hydrogenated oils and fats, and fatty acids having a melting point of more than 40 °C .
  • the present invention relates to a method for preparing double-coated ruminally protected choline, which is not degraded by microorganisms, pH and water in the rumen, and thus can be stably delivered and absorbed in lower digestive tract.
  • the concentrated liquid choline is immobilized on a carrier, and water is then removed therefrom, to prepare carrier-immobilized choline.
  • the concentrated liquid choline is preferably one or a mixture of two or more selected from the group consisting of choline chloride, choline dihydrogen citrate, tricholine citrate and choline bitartrate.
  • the carrier is preferably a water- insoluble carrier selected from the group consisting of silica, cellulose, starch and zeolite. More preferably, the carrier is silica.
  • the carrier-immobilized choline is encapsulated with a binder/coating material to prepare granular or needle-shaped, first encapsulated choline.
  • the encapsulation of the carrier-immobilized choline is performed by mixing and agitating the immobilized choline and the binder/coating material in a mixer such as a ribbon mixer, general agitator, a homo-mixer or a V-type mixer, and then extruding the mixture through an extruder or a plodder.
  • the choline and the binder/coating material are preferably mixed with each other at a weight ratio of 1:0.1-2.0.
  • a perforated plate in the extruder has preferably a size of 0.5-2.5 mm, and an extrusion barrel in the extruder is preferably maintained at a temperature of 10-60 " C .
  • the binder/coating material is preferably any one or a mixture of two or more selected from the group consisting of monovalent soap, divalent soap, and fatty acid having a melting point of more than 40 ° C .
  • the monovalent soap is preferably sodium soap or potassium soap
  • the divalent soap is preferably selected from the group consisting of beeswax, PEG, surfactants, calcium stearate, zinc stearate and magnesium stearate.
  • the fatty acid having a melting point of more than 40 °C is preferably selected from the group consisting of hydrogenated oil, extremely hydrogenated oil, palmitic acid and stearic acid. More preferred is hydrogenated oil.
  • encapsulated choline having a particle size of 0.5-2.0 mm is screened.
  • the screened encapsulated choline is further encapsulated with any one or a combination of two or more selected form the group consisting of extremely hydrogenated oils and fats, and fatty acids having a melting point of more than 40 °C , thus preparing double-coated ruminally protected choline.
  • the content of the double coating layer in the finally prepared, double-coated ruminally protected choline is preferably 15-55% (w/w).
  • the double-coated ruminally protective choline prepared according to the present invention has advantages in that it does not show formation of micropores which is a problem in the products prepared according to the prior commercial cold spray process, and has a uniform shape.
  • the products prepared according to the cold spray process has shortcomings in that choline is located on the particle surface, and thus has reduced stability in the rumen, whereas the double-coated ruminally protected choline prepared according to the present invention has advantages in that, because the choline- containing product subjected to the first coating (encapsulation) process is further subjected to the second coating (encapsulation) process, the possibility of choline distributed on the product surface is eliminated, and thus most of the choline contained in the product can be maintained stable.
  • the present invention does not entail the use of a refrigerant used in the cold spray process, thus reducing production expenses, and requires low costs of factory site and installation, so that it reduces the production cost of the product.
  • the inventive ruminally protected choline can contribute to the development of feed additives capable of producing functional livestock.
  • Concentrated liquid choline was immobilized on a carrier such as silica, cellulose, starch, zeolite or the like, and then water was removed therefrom, thus preparing carrier-immobilized choline .
  • a binder/coating material such as beeswax, PEG, hydrogenated oil or the like was added to the carrier-immobilized choline, and then mixed and agitated in a mixer such as a ribbon mixer, a general agitator, a homo-mixer, a V-type mixer or the like.
  • the mixture was passed through an extruder or a plodder, thus preparing first encapsulated (coated) choline.
  • the temperature of an extrusion barrel in the extruder or the plodder was maintained at 10-60°C .
  • encapsulated choline having a particle size of 0.5-2.0 mm was screened.
  • one or a combination of two or more selected from the group consisting of extremely hydrogenated oils and fats, and fatty acids having a melting point of more than 40 ° C for example, hydrogenated vegetable oil, hydrogenated corn oil, hydrogenated cottonseed oil, hydrogenated peanut oil, hydrogenated palm kernel oil, hydrogenated palm oil, hydrogenated palm stearic acid, hydrogenated sunflower oil, hydrogenated canola oil, palmitic acid and stearic acid, was added.
  • the mixture containing the first encapsulated choline was subjected to a second encapsulation process in an encapsulating device, such as a high-speed mixer, a ribbon mixer, roedige mixer, or a fluidized bed processor, thus preparing double-coated ruminally protected choline.
  • an encapsulating device such as a high-speed mixer, a ribbon mixer, roedige mixer, or a fluidized bed processor, thus preparing double-coated ruminally protected choline.
  • first encapsulating material 1 kg of liquid hydrogenated oil (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate having a hole diameter of 1.0 mm, to prepare a first encapsulated material.
  • 1 kg of the prepared first encapsulated material was placed in a high-speed mixer, to which 670 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 °C , was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 40.1% based on the total weight of the finally produced, second encapsulated material.
  • first encapsulating material 1 kg of liquid hydrogenated oil (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 1.2 mm, to prepare a first encapsulated material.
  • 1 kg of the prepared first encapsulated material was placed in a highspeed mixer, to which 670 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 °C , was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 40.1% based on the total weight of the finally produced, second encapsulated material.
  • Test Example 3 1 kg of liquid hydrogenated oil (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 1.5 mm, to prepare a first encapsulated material. 1 kg of the prepared first encapsulated material was placed in a high- speed mixer, to which 670 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 °C, was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 40.1% based on the total weight of the finally produced, second encapsulated material.
  • first encapsulating material 1 kg of liquid hydrogenated oil (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 2.0 mm, to prepare a first encapsulated material.
  • 1 kg of the prepared first encapsulated material was placed in a highspeed mixer, to which 670 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 °C , was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 40.1% based on the total weight of the finally produced, second encapsulated material.
  • Test Example 5 1 kg of monovalent soap (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 1.0 mm, to prepare a first encapsulated material. 1 kg of the prepared first encapsulated material was placed in a high-speed mixer, to which 670 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 ° C , was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 40.1% based on the total weight of the finally produced, second encapsulated material.
  • first encapsulating material 1 kg of PEG (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 1.0 mm, to prepare a first encapsulated material.
  • 1 kg of the prepared first encapsulated material was placed in a high-speed mixer, to which 670 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 ° C , was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 40.1% based on the total weight of the finally produced, second encapsulated material.
  • first encapsulating material 1 kg
  • binder/coating material 1 kg
  • 1 kg of Tween 80 (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 1.0 mm, to prepare a first encapsulated material.
  • 1 kg of the prepared first encapsulated material was placed in a high-speed mixer, to which 670 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 " C, was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 40.1% based on the total weight of the finally produced, second encapsulated material.
  • Test Example 8 1 kg of Span 80 (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 1.0 mm, to prepare a first encapsulated material. 1 kg of the prepared first encapsulated material was placed in a high-speed mixer, to which 670 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 ° C , was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 40.1% based on the total weight of the finally produced, second encapsulated material.
  • first encapsulating material 1 kg of liquid hydrogenated oil (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 1.2 mm, to prepare a first encapsulated material.
  • 1 kg of the prepared first encapsulated material was placed in a highspeed mixer, to which 500 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 °C, was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 33.3% based on the total weight of the finally produced, second encapsulated material.
  • Test Example 10 1 kg of liquid hydrogenated oil (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 1.2 mm, to prepare a first encapsulated material. 1 kg of the prepared first encapsulated material was placed in a highspeed mixer, to which 350 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 °C, was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 25.9% based on the total weight of the finally produced, second encapsulated material.
  • first encapsulating material 1 kg of liquid hydrogenated oil (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 1.2 mm, to prepare a first encapsulated material.
  • 1 kg of the prepared first encapsulated material was placed in a highspeed mixer, to which 250 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 °C , was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 20.2% based on the total weight of the finally produced, second encapsulated material.
  • first encapsulating material 1 kg of liquid hydrogenated oil (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 1.2 mm, to prepare a first encapsulated material.
  • 1 kg of the prepared first encapsulated material was placed in a highspeed mixer, to which 150 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 "C, was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 13.0% based on the total weight of the finally produced, second encapsulated material.
  • Test Example 13 1 kg of liquid hydrogenated oil (first encapsulating material; binder/coating material) was mixed with 5 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 1.2 mm, to prepare a first encapsulated material. 1 kg of the prepared first encapsulated material was placed in a high- speed mixer, to which 50 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 °C, was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 4.8% based on the total weight of the finally produced, second encapsulated material.
  • first encapsulating material binder/coating material
  • first encapsulating material binder/coating material
  • choline a perforated plate having a hole diameter of 2.0 mm
  • 1 kg of the prepared first encapsulated material was placed in a highspeed mixer, to which 670 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 °C , was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 40.1% based on the total weight of the finally produced, second encapsulated material.
  • Test Example 15 3 kg of liquid hydrogenated oil (first encapsulating material; binder/coating material) was mixed with 3 kg of choline, and the mixture was extruded through a perforated plate having a hole diameter of 2.0 mm, to prepare a first encapsulated material.
  • 1 kg of the prepared first encapsulated material was placed in a highspeed mixer, to which 670 g of any one or a mixture of two or more selected from among liquid hydrogenated oils, and fatty acids having a melting point of more than 40 "C , was then added as a second encapsulating material, thus preparing a second encapsulated material.
  • the amount of the second encapsulating material was 40.1% based on the total weight of the finally produced, second encapsulated material.
  • Example 2 in vitro loss of ruminally protected choline
  • each of the prepared ruminally protected cholines was placed in a test tube, to which distilled water was added, to prepare a 5% choline aqueous solution.
  • the aqueous solution was stirred using the JEIO TECH MC-I l multi-stirrer at 40 0 C ⁇ 0.1 ° C for 2 hours.
  • the stirred aqueous solution was filtered through Whatman paper NO.3 to collect only a solid.
  • the solid was dried at 45 °C ⁇ 0.1 ° C for at least 12 hours to completely remove the remaining water.
  • the dried sample was cooled to room temperature in the Sanpla Dry Keeper, and 300 mg of the cooled sample was completely dissolved in a mixed solvent of chloroform and methanol (1:1 v/v).
  • the content of choline in the solution was measured using a 5% K 2 CrO 4 solution as indicator, and a 0.1 N AgNO 3 solution as titrant.
  • the double-coated ruminally protected choline prepared by selecting liquid hydrogenated oil as the first encapsulating material, mixing choline with the first encapsulating material at a ratio of 1 :0.5 or 1 :1, subjecting the mixture to the first encapsulating process and subjecting the first encapsulated material to the second encapsulating process using an increased amount of the second encapsulating material, showed minimized in vitro loss.
  • Example 3 Feeding test of ruminally protected choline
  • Example 15 A feeding test in vivo was conducted on the ruminally protected choline prepared in Example 15, which showed low in vitro loss in the test of Example 2 and had suitable size and a high choline content.
  • diary cows in middle lactation were selected as test animals and fed a sufficient amount of a total mixed ration of compound feed and bulky feed two times daily at 12-hr intervals.
  • the ruminally protected choline (25% of choline content) was additionally fed to the cows in an amount of 40 g (10 g based on choline) per cow when feeding the total mixed ration.
  • the cows were milked two times daily and the amount of milk produced was measured.
  • a milk sample was taken from the produced milk, and the milk components and somatic cell number of the milk sample were measured using an automatic analyzer (LactoScopeR, MK2, Delta Instruments, The Netherlands).
  • the present invention provides the method for preparing double-coated ruminally protected choline.
  • the ruminally protected choline prepared according to the present invention is not degraded by microorganisms, pH and water in the rumen, and thus can be stably delivered and absorbed in the lower digestive tract.
  • it when it is used in the breeding of ruminant livestock, it can increase the nutrient availability of the ruminant livestock, thus leading to an increase in the livestock production, and makes it possible to produce functional livestock producing choline-rich milk.
  • the inventive method for preparing the ruminally protected choline can be applied to protect various components from ruminal degradation and can be used for the production of a variety of novel feed additives.

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Abstract

La présente invention concerne un procédé de préparation de choline à double enrobage pour la protection contre la dégradation ruminale, qui n'est pas dégradée par des micro-organismes, le pH et l'eau dans le rumen, et peut donc être administrée et absorbée de manière stable dans la voie digestive inférieure. Plus particulièrement, le procédé comprend les étapes suivantes: (a) la préparation de choline à support immobilisé par l'immobilisation de choline liquide concentrée sur le support suivie de l'élimination d'eau à partir de la choline immobilisée; (b) la préparation d'une première choline encapsulée par l'encapsulation de la choline à support immobilisé avec un liant/enrobage; (c) le criblage de choline encapsulée ayant une taille comprise entre 0,5 et 2,0 mm à partir de la première choline encapsulée; et (d) l'encapsulation supplémentaire de la choline encapsulée avec un élément ou une combinaison d'au moins deux éléments choisi(s) parmi le groupe constitué d'huiles et de graisses très hydrogénées, et d'acides gras ayant une température de fusion supérieure à 40°C.
PCT/KR2006/004773 2006-05-02 2006-11-14 Procédé de préparation de choline à protection contre la destruction ruminale Ceased WO2007126191A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN2006800544697A CN101431903B (zh) 2006-05-02 2006-11-14 制备反刍保护的胆碱的方法
JP2009509394A JP5159766B2 (ja) 2006-05-02 2006-11-14 反芻胃保護コリンの製造方法

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9186297B2 (en) 2005-09-12 2015-11-17 Abela Pharmaceuticals, Inc. Materials for facilitating administration of dimethyl sulfoxide (DMSO) and related compounds
US9186472B2 (en) 2005-09-12 2015-11-17 Abela Pharmaceuticals, Inc. Devices for removal of dimethyl sulfoxide (DMSO) or related compounds or associated odors and methods of using same
US20160037805A1 (en) * 2009-04-23 2016-02-11 H.J. Baker & Bro., Inc. Granular Feed Supplement
US9427419B2 (en) 2005-09-12 2016-08-30 Abela Pharmaceuticals, Inc. Compositions comprising dimethyl sulfoxide (DMSO)
US9839609B2 (en) 2009-10-30 2017-12-12 Abela Pharmaceuticals, Inc. Dimethyl sulfoxide (DMSO) and methylsulfonylmethane (MSM) formulations to treat osteoarthritis
CN113331306A (zh) * 2021-06-03 2021-09-03 北京中科万联科技有限公司 基于丝兰提高畜禽生理机能的添加剂及其制备方法、应用
EP4035537A4 (fr) * 2019-09-26 2023-09-27 Ajinomoto Co., Inc. Composition d'additif alimentaire pour ruminants
CN117121981A (zh) * 2023-08-29 2023-11-28 辽宁亚禾营养科技有限责任公司 过瘤胃保护亚麻酸的制备方法

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101069118B1 (ko) 2008-10-22 2011-09-30 경상대학교산학협력단 장용해성 염화콜린의 제조방법 및 이의 방법에 따라 제조되는 염화콜린
CN101897463A (zh) * 2009-06-01 2010-12-01 王中心 反刍动物过瘤胃产品的生产方法
KR101219740B1 (ko) 2010-06-07 2013-01-11 대한민국 콜린 강화 우유의 생산을 위한 반추위 보호 콜린의 제조방법 및 이로 인한 반추위 보호 콜린
CN103549153A (zh) * 2013-11-11 2014-02-05 张维 一种过瘤胃氯化胆碱颗粒及其生产方法
WO2015115618A1 (fr) 2014-01-31 2015-08-06 森下仁丹株式会社 Agent administré oralement pour ruminants et aliment pour ruminants contenant celui-ci
CN104719670B (zh) * 2015-03-24 2018-04-27 河北碧隆化工科技有限公司 一种高含量反刍动物过瘤胃氯化胆碱颗粒及其制备方法
CN104872395B (zh) * 2015-06-05 2018-06-19 西双版纳华坤生物科技有限责任公司 一种富含ω-3脂肪酸和共轭亚油酸的牛奶及其生产方法
CN105166413A (zh) * 2015-10-10 2015-12-23 山西农业大学 肉牛过瘤胃核黄素添加剂及其制备方法
CN112167165B (zh) * 2020-09-29 2022-02-11 厦门汇盛生物有限公司 一种复配过瘤胃多不饱和脂肪酸粉的制备及其应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4713245A (en) * 1984-06-04 1987-12-15 Mitsui Toatsu Chemicals, Incorporated Granule containing physiologically-active substance, method for preparing same and use thereof
US4876097A (en) * 1984-12-20 1989-10-24 Rhone-Poulenc Sante Compositions for coating feeding stuff additives intended for ruminants and feeding stuff additives thus coated
WO1996008168A1 (fr) * 1994-09-16 1996-03-21 Imperial Chemical Industries Plc Aliments pour animaux et additifs
KR0163831B1 (ko) * 1995-03-18 1998-11-16 비비바흐, 카르그 반추위내의 분해에 대해 보호된 라이신의 제조방법 및 이를 포함한 동물사료
US6013286A (en) * 1991-05-29 2000-01-11 Balchem Corporation Encapsulated bioactive substances
US6797291B2 (en) * 2002-01-09 2004-09-28 Balchem Corporation Stable hygroscopic compositions and methods for stabilizing hygroscopic ingredients

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1331713C (fr) * 1988-12-29 1994-08-30 Hitoshi Iijima Composition granulaire pour ruminants
WO1994015480A1 (fr) * 1992-12-30 1994-07-21 Morgan Manufacturing Co., Inc. Composition et procede de production de lait chez les ruminants
JPH06339343A (ja) * 1993-04-08 1994-12-13 Ajinomoto Co Inc 反すう動物用飼料添加物
JPH0764547A (ja) * 1993-08-30 1995-03-10 Roland Corp マーカー・クォンタイズ装置
JPH0787900A (ja) * 1993-09-27 1995-04-04 Ajinomoto Co Inc 反すう動物用飼料添加組成物
JPH08336360A (ja) * 1995-03-17 1996-12-24 Kanagawa Kagaku Kenkyusho:Kk 反芻動物用飼料組成物及びそれを用いる飼養方法
US5807594A (en) * 1997-02-26 1998-09-15 Ducoa, L.P. Method for enhancing feed efficiency in ruminants with an encapsulating choline composition
WO2002082921A1 (fr) * 2001-04-16 2002-10-24 Jubilant Organosys Limited Composition soustraite a la degradation ruminale contenant une substance bio-active et procede de preparation associe
DE10244397A1 (de) * 2002-09-24 2004-04-01 Basf Ag Cholinascorbat-Formulierungen
CN1561979A (zh) * 2004-04-21 2005-01-12 浙江大学 过瘤胃氯化胆碱的微胶囊及其制备方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4713245A (en) * 1984-06-04 1987-12-15 Mitsui Toatsu Chemicals, Incorporated Granule containing physiologically-active substance, method for preparing same and use thereof
US4876097A (en) * 1984-12-20 1989-10-24 Rhone-Poulenc Sante Compositions for coating feeding stuff additives intended for ruminants and feeding stuff additives thus coated
US6013286A (en) * 1991-05-29 2000-01-11 Balchem Corporation Encapsulated bioactive substances
WO1996008168A1 (fr) * 1994-09-16 1996-03-21 Imperial Chemical Industries Plc Aliments pour animaux et additifs
KR0163831B1 (ko) * 1995-03-18 1998-11-16 비비바흐, 카르그 반추위내의 분해에 대해 보호된 라이신의 제조방법 및 이를 포함한 동물사료
US6797291B2 (en) * 2002-01-09 2004-09-28 Balchem Corporation Stable hygroscopic compositions and methods for stabilizing hygroscopic ingredients

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9186297B2 (en) 2005-09-12 2015-11-17 Abela Pharmaceuticals, Inc. Materials for facilitating administration of dimethyl sulfoxide (DMSO) and related compounds
US9186472B2 (en) 2005-09-12 2015-11-17 Abela Pharmaceuticals, Inc. Devices for removal of dimethyl sulfoxide (DMSO) or related compounds or associated odors and methods of using same
US9427419B2 (en) 2005-09-12 2016-08-30 Abela Pharmaceuticals, Inc. Compositions comprising dimethyl sulfoxide (DMSO)
US20160037805A1 (en) * 2009-04-23 2016-02-11 H.J. Baker & Bro., Inc. Granular Feed Supplement
US9839609B2 (en) 2009-10-30 2017-12-12 Abela Pharmaceuticals, Inc. Dimethyl sulfoxide (DMSO) and methylsulfonylmethane (MSM) formulations to treat osteoarthritis
US9855212B2 (en) 2009-10-30 2018-01-02 Abela Pharmaceuticals, Inc. Dimethyl sulfoxide (DMSO) or DMSO and methylsulfonylmethane (MSM) formulations to treat infectious diseases
US10596109B2 (en) 2009-10-30 2020-03-24 Abela Pharmaceuticals, Inc. Dimethyl sulfoxide (DMSO) or DMSO and methylsulfonylmethane (MSM) formulations to treat infectious diseases
EP4035537A4 (fr) * 2019-09-26 2023-09-27 Ajinomoto Co., Inc. Composition d'additif alimentaire pour ruminants
CN113331306A (zh) * 2021-06-03 2021-09-03 北京中科万联科技有限公司 基于丝兰提高畜禽生理机能的添加剂及其制备方法、应用
CN117121981A (zh) * 2023-08-29 2023-11-28 辽宁亚禾营养科技有限责任公司 过瘤胃保护亚麻酸的制备方法

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JP2009535056A (ja) 2009-10-01

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