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WO2007123684A3 - Procedes et dispositifs permettant de decongeler et/ou d'obtenir une image d'echantillons biologiques congeles - Google Patents

Procedes et dispositifs permettant de decongeler et/ou d'obtenir une image d'echantillons biologiques congeles Download PDF

Info

Publication number
WO2007123684A3
WO2007123684A3 PCT/US2007/007915 US2007007915W WO2007123684A3 WO 2007123684 A3 WO2007123684 A3 WO 2007123684A3 US 2007007915 W US2007007915 W US 2007007915W WO 2007123684 A3 WO2007123684 A3 WO 2007123684A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
thawing
devices
imaging
biological samples
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/007915
Other languages
English (en)
Other versions
WO2007123684A2 (fr
Inventor
Mark A Voelker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lineage Cell Therapeutics Inc
Original Assignee
Biotime Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/601,442 external-priority patent/US20070231787A1/en
Application filed by Biotime Inc filed Critical Biotime Inc
Publication of WO2007123684A2 publication Critical patent/WO2007123684A2/fr
Anticipated expiration legal-status Critical
Publication of WO2007123684A3 publication Critical patent/WO2007123684A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42Low-temperature sample treatment, e.g. cryofixation
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10Preservation of living parts
    • A01N1/16Physical preservation processes
    • A01N1/162Temperature processes, e.g. following predefined temperature changes over time
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10Preservation of living parts
    • A01N1/16Physical preservation processes
    • A01N1/165Pressure processes, e.g. following predefined pressure changes over time
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346Heating or cooling arrangements

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne des procédés permettant de congeler, décongeler et d'obtenir des images d'un échantillon biologique et ce, une ou plusieurs fois. Lesdits procédés sont effectués de manière à permettre de congeler, décongeler et/ou obtenir des images de l'échantillon biologique, tout en conservant l'intégrité structurelle et/ou la viabilité de l'échantillon. Des modes de réalisation des procédés consistent à congeler, décongeler et/ou obtenir des images d'un échantillon biologique sous pression de manière à pouvoir conserver l'intégrité structurelle et/ou la viabilité de l'échantillon. L'invention porte aussi sur des dispositifs à utiliser lors de l'application des présents procédés.
PCT/US2007/007915 2006-04-04 2007-03-30 Procedes et dispositifs permettant de decongeler et/ou d'obtenir une image d'echantillons biologiques congeles Ceased WO2007123684A2 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US78954106P 2006-04-04 2006-04-04
US60/789,541 2006-04-04
US78954106A 2006-11-17 2006-11-17
US11/789,541 2006-11-17
US11/601,442 US20070231787A1 (en) 2006-04-04 2006-11-17 Methods and devices for imaging and manipulating biological samples
US11/601,442 2006-11-17

Publications (2)

Publication Number Publication Date
WO2007123684A2 WO2007123684A2 (fr) 2007-11-01
WO2007123684A3 true WO2007123684A3 (fr) 2008-12-11

Family

ID=38625465

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/007915 Ceased WO2007123684A2 (fr) 2006-04-04 2007-03-30 Procedes et dispositifs permettant de decongeler et/ou d'obtenir une image d'echantillons biologiques congeles

Country Status (1)

Country Link
WO (1) WO2007123684A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9023273B2 (en) * 2009-08-18 2015-05-05 Lifecell Corporation Method for processing tissues
CN106404614A (zh) * 2016-08-25 2017-02-15 哈尔滨商业大学 一种冰结构蛋白的冰晶观察方法
CN107402213B (zh) * 2017-08-29 2023-09-22 苏州欧谱曼迪科技有限公司 探头、探头装置以及显微系统
CN111158133A (zh) * 2020-02-17 2020-05-15 广州市妇女儿童医疗中心(广州市妇幼保健院、广州市儿童医院、广州市妇婴医院、广州市妇幼保健计划生育服务中心) 体式显微镜及冷冻解冻操作装置
CN118443669B (zh) * 2024-07-08 2024-09-13 深圳市中医院 一种生物组织低温切片成像设备

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage

Also Published As

Publication number Publication date
WO2007123684A2 (fr) 2007-11-01

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