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WO2007110592A3 - Fermentation process for the production of ethanol - Google Patents

Fermentation process for the production of ethanol Download PDF

Info

Publication number
WO2007110592A3
WO2007110592A3 PCT/GB2007/001018 GB2007001018W WO2007110592A3 WO 2007110592 A3 WO2007110592 A3 WO 2007110592A3 GB 2007001018 W GB2007001018 W GB 2007001018W WO 2007110592 A3 WO2007110592 A3 WO 2007110592A3
Authority
WO
WIPO (PCT)
Prior art keywords
fermentation
ethanol
bacterium
production
fermentation process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2007/001018
Other languages
French (fr)
Other versions
WO2007110592A2 (en
Inventor
Neil Bookless
Brian Hartley
Namdar Baghaei-Yazdi
Muhammed Javed
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ELSWORTH ETHANOL Co Ltd
Original Assignee
ELSWORTH ETHANOL Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0605890.3A external-priority patent/GB0605890D0/en
Priority claimed from GBGB0605889.5A external-priority patent/GB0605889D0/en
Application filed by ELSWORTH ETHANOL Co Ltd filed Critical ELSWORTH ETHANOL Co Ltd
Publication of WO2007110592A2 publication Critical patent/WO2007110592A2/en
Publication of WO2007110592A3 publication Critical patent/WO2007110592A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method of producing ethanol using a bacterial fermentation process is described. Fermentation is carried out using a first bacterium with desired ethanol producing pathway activity to produce ethanol. Process conditions are controlled to prevent saturation of pathway flux, or to restore pathway flux following saturation, thereby preventing or reducing the risk of takeover of fermentation by a second bacterium with undesired fermentation activity. A micro-organism with improved fermentation properties is also described.
PCT/GB2007/001018 2006-03-24 2007-03-21 Fermentation process for the production of ethanol Ceased WO2007110592A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB0605890.3A GB0605890D0 (en) 2006-03-24 2006-03-24 Enhancemeny Of Microbial Ethanol Production
GBGB0605889.5A GB0605889D0 (en) 2006-03-24 2006-03-24 Regulation Of Thermophile Ethanol Fermentation
GB0605890.3 2006-03-24
GB0605889.5 2006-03-24

Publications (2)

Publication Number Publication Date
WO2007110592A2 WO2007110592A2 (en) 2007-10-04
WO2007110592A3 true WO2007110592A3 (en) 2007-11-29

Family

ID=38267688

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/001018 Ceased WO2007110592A2 (en) 2006-03-24 2007-03-21 Fermentation process for the production of ethanol

Country Status (1)

Country Link
WO (1) WO2007110592A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0605890D0 (en) * 2006-03-24 2006-05-03 Bioconversion Technologies Ltd Enhancemeny Of Microbial Ethanol Production
GB2469758A (en) 2008-02-28 2010-10-27 Green Biologics Ltd Process for producing butyric acid and other acids using electrochemical reactor
GB0806093D0 (en) * 2008-04-03 2008-05-14 Green Biologics Ltd Production of butanol
GB2505502A (en) * 2012-09-03 2014-03-05 Ensus Ltd Hydrolysis and fermentation process for producing bioethanol
CN102965397A (en) * 2012-11-16 2013-03-13 大连理工大学 Method for automatically controlling repeated-batch ethanol fermentation process based on redox potential

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD212053A1 (en) * 1982-12-03 1984-08-01 Akad Wissenschaften Ddr PROCESS FOR OBTAINING ETHANOL
WO1988009379A2 (en) * 1987-05-27 1988-12-01 Elsworth Biotechnology Limited Thermophilic ethanol production
WO2002029030A2 (en) * 2000-10-06 2002-04-11 Elsworth Biotechnology Limited Ethanol production
US20050042736A1 (en) * 2003-08-22 2005-02-24 Ka-Yiu San High molar succinate yield bacteria by increasing the intracellular NADH availability

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD212053A1 (en) * 1982-12-03 1984-08-01 Akad Wissenschaften Ddr PROCESS FOR OBTAINING ETHANOL
WO1988009379A2 (en) * 1987-05-27 1988-12-01 Elsworth Biotechnology Limited Thermophilic ethanol production
WO2002029030A2 (en) * 2000-10-06 2002-04-11 Elsworth Biotechnology Limited Ethanol production
US20050042736A1 (en) * 2003-08-22 2005-02-24 Ka-Yiu San High molar succinate yield bacteria by increasing the intracellular NADH availability

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BERRÍOS-RIVERA SUSANA J ET AL: "Metabolic engineering of Escherichia coli: increase of NADH availability by overexpressing an NAD(+)-dependent formate dehydrogenase.", METABOLIC ENGINEERING JUL 2002, vol. 4, no. 3, July 2002 (2002-07-01), pages 217 - 229, XP002362652, ISSN: 1096-7176 *
SAN MARTIN R ET AL: "PATHWAYS OF ETHANOL PRODUCTION FROM SUCROSE BY A MUTANT THERMOPHILIC BACILLUS IN CONTINUOUS CULTURE", JOURNAL OF GENERAL MICROBIOLOGY, SOCIETY FOR MICROBIOLOGY, READING, GB, vol. 139, no. 5, 1993, pages 1033 - 1040, XP001247278, ISSN: 0022-1287 *

Also Published As

Publication number Publication date
WO2007110592A2 (en) 2007-10-04

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