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WO2007107775A8 - Preparation of oligonucleotides with photoswitchable properties - Google Patents

Preparation of oligonucleotides with photoswitchable properties Download PDF

Info

Publication number
WO2007107775A8
WO2007107775A8 PCT/GB2007/001056 GB2007001056W WO2007107775A8 WO 2007107775 A8 WO2007107775 A8 WO 2007107775A8 GB 2007001056 W GB2007001056 W GB 2007001056W WO 2007107775 A8 WO2007107775 A8 WO 2007107775A8
Authority
WO
WIPO (PCT)
Prior art keywords
compounds
photoswitchable
modulatable
properties
photo
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2007/001056
Other languages
French (fr)
Other versions
WO2007107775A3 (en
WO2007107775A2 (en
Inventor
Joseph S Vyle
James Robert Tucker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Queens University of Belfast
Original Assignee
Queens University of Belfast
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Queens University of Belfast filed Critical Queens University of Belfast
Publication of WO2007107775A2 publication Critical patent/WO2007107775A2/en
Publication of WO2007107775A3 publication Critical patent/WO2007107775A3/en
Anticipated expiration legal-status Critical
Publication of WO2007107775A8 publication Critical patent/WO2007107775A8/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Linkers which provide for the attachment of photoswitchable compounds to nucleic acid are provided. These linkers can be used to provide photo-modulatable compounds wherein the chemical and biological properties of polynucleic acid incorporating these photo-modulatable compounds can be modulated by light. Further, methods for modulation of hybridisation of polynucleic acid sequences and binding of factors, for example polymerases to polynucleic acid sequences incorporating said photo-modulatable compounds are provided.
PCT/GB2007/001056 2006-03-23 2007-03-23 Preparation of oligonucleotides with photoswitchable properties Ceased WO2007107775A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0605787A GB0605787D0 (en) 2006-03-23 2006-03-23 "Preparation of oligonucleotides with photoswitchable properties
GB0605787.1 2006-03-23

Publications (3)

Publication Number Publication Date
WO2007107775A2 WO2007107775A2 (en) 2007-09-27
WO2007107775A3 WO2007107775A3 (en) 2008-03-27
WO2007107775A8 true WO2007107775A8 (en) 2009-07-16

Family

ID=36383996

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/001056 Ceased WO2007107775A2 (en) 2006-03-23 2007-03-23 Preparation of oligonucleotides with photoswitchable properties

Country Status (2)

Country Link
GB (1) GB0605787D0 (en)
WO (1) WO2007107775A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105567673A (en) * 2014-10-31 2016-05-11 希森美康株式会社 Method for amplifying nucleic acid

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201220282D0 (en) * 2012-11-12 2012-12-26 Univ Edinburgh Electrochemical method
CN106905399B (en) * 2017-03-27 2019-07-09 武汉大学 A kind of light control method that double-stranded DNA B-Z conformation is mutually converted
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200528467A (en) * 2004-02-27 2005-09-01 Japan Science & Tech Agency DNA enzyme and method of controlling the activity thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105567673A (en) * 2014-10-31 2016-05-11 希森美康株式会社 Method for amplifying nucleic acid
CN105567673B (en) * 2014-10-31 2018-09-04 希森美康株式会社 The method of amplification of nucleic acid

Also Published As

Publication number Publication date
WO2007107775A3 (en) 2008-03-27
GB0605787D0 (en) 2006-05-03
WO2007107775A2 (en) 2007-09-27

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