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WO2007034071A3 - Procede de preparation d' un echantillon - Google Patents

Procede de preparation d' un echantillon Download PDF

Info

Publication number
WO2007034071A3
WO2007034071A3 PCT/FR2006/002149 FR2006002149W WO2007034071A3 WO 2007034071 A3 WO2007034071 A3 WO 2007034071A3 FR 2006002149 W FR2006002149 W FR 2006002149W WO 2007034071 A3 WO2007034071 A3 WO 2007034071A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
compressed fluid
preparing
contacting
solid matrix
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2006/002149
Other languages
English (en)
Other versions
WO2007034071A2 (fr
Inventor
Michel Perrut
Frederic Imbs
Frantz Deschamps
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Histolex
Original Assignee
Histolex
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Histolex filed Critical Histolex
Priority to CN2006800433980A priority Critical patent/CN101351692B/zh
Priority to EP06808171A priority patent/EP1949068A2/fr
Priority to US12/094,158 priority patent/US8409793B2/en
Publication of WO2007034071A2 publication Critical patent/WO2007034071A2/fr
Publication of WO2007034071A3 publication Critical patent/WO2007034071A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0203—Solvent extraction of solids with a supercritical fluid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Procédé de traitement d'un échantillon constitué de tissu biologique inclus dans une matrice solide. Ce procédé est caractérisé en ce qu'il comporte les étapes consistant à : mettre l'échantillon en contact avec un flux d'un premier fluide comprimé dans lequel ladite matrice support est soluble, de façon à provoquer l'extraction de ladite matrice solide, mettre l'échantillon en contact avec un second fluide comprimé, apte à imprégner le tissu par une substance aqueuse ou hydrosoluble, évacuer le second fluide comprimé de manière graduelle et contrôlée, récupérer l'échantillon.
PCT/FR2006/002149 2005-09-21 2006-09-20 Procede de preparation d' un echantillon Ceased WO2007034071A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN2006800433980A CN101351692B (zh) 2005-09-21 2006-09-20 制备样品的方法
EP06808171A EP1949068A2 (fr) 2005-09-21 2006-09-20 Procede de preparation d' un echantillon
US12/094,158 US8409793B2 (en) 2005-09-21 2006-09-20 Method for preparing a sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0509635 2005-09-21
FR0509635A FR2891052B1 (fr) 2005-09-21 2005-09-21 Procede de preparation d'un echantillon

Publications (2)

Publication Number Publication Date
WO2007034071A2 WO2007034071A2 (fr) 2007-03-29
WO2007034071A3 true WO2007034071A3 (fr) 2007-05-24

Family

ID=36616805

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2006/002149 Ceased WO2007034071A2 (fr) 2005-09-21 2006-09-20 Procede de preparation d' un echantillon

Country Status (5)

Country Link
US (1) US8409793B2 (fr)
EP (1) EP1949068A2 (fr)
CN (1) CN101351692B (fr)
FR (1) FR2891052B1 (fr)
WO (1) WO2007034071A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5227079B2 (ja) * 2008-05-19 2013-07-03 サクラ精機株式会社 組織片処理装置
US10092905B2 (en) 2012-06-22 2018-10-09 Leica Biosystems Nussloch Gmbh Tissue sample container and methods
ES2733446T3 (es) 2012-06-22 2019-11-29 Leica Biosystems Nussloch Gmbh Dispositivo de transporte de muestras de tejido de biopsia
US9389154B2 (en) 2013-03-15 2016-07-12 Leica Biosystems Nussloch Gmbh Tissue cassette with biasing element
US9097629B2 (en) 2013-03-15 2015-08-04 Leica Biosystems Nussloch Gmbh Tissue cassette with retractable member
US9052256B2 (en) 2013-03-15 2015-06-09 Leica Biosystems Nussloch Gmbh Method for processing and embedding tissue
JP6841609B2 (ja) 2015-07-10 2021-03-10 ３スキャン インコーポレイテッド 組織学的染色の空間的多重化
US9846109B2 (en) * 2015-10-27 2017-12-19 Sgs North America Inc. Fluid sampling
JP7623471B2 (ja) * 2020-08-10 2025-01-28 ライカ ビオズュステムス ヌスロッホ ゲーエムベーハー キシレンフリーの組織処理装置及び組織を処理するための方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030072677A1 (en) * 2001-10-17 2003-04-17 Ralph Kafesjian Supercritical fluid extraction process for tissue preparation
WO2005001437A1 (fr) * 2003-06-30 2005-01-06 Academisch Ziekenhuis Groningen Methode de traitement histologique

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HK1045189A1 (zh) * 1999-02-19 2002-11-15 分析科学公司 整体染色和包埋法相结合
FR2798863B1 (fr) 1999-09-27 2001-12-28 Separex Sa Procede et installation de mise a l'etat adsorbe sur un support poreux de composes actifs contenus dans un produit

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030072677A1 (en) * 2001-10-17 2003-04-17 Ralph Kafesjian Supercritical fluid extraction process for tissue preparation
WO2005001437A1 (fr) * 2003-06-30 2005-01-06 Academisch Ziekenhuis Groningen Methode de traitement histologique

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FRAYSSINET P ET AL: "Histological integration of allogeneic cancellous bone tissue treated by supercritical CO2 implanted in sheep bones", BIOMATERIALS, ELSEVIER SCIENCE PUBLISHERS BV., BARKING, GB, vol. 19, no. 24, December 1998 (1998-12-01), pages 2247 - 2253, XP004168858, ISSN: 0142-9612 *
RIBEIRO DOS SANTOS I ET AL: "A SUPERCRITICAL FLUID-BASED COATING TECHNOLOGY. 2: SOLUBILITY CONSIDERATIONS", JOURNAL OF MICROENCAPSULATION, TAYLOR AND FRANCIS, BASINGSTOKE, GB, vol. 20, no. 1, January 2003 (2003-01-01), pages 97 - 109, XP001142295, ISSN: 0265-2048 *

Also Published As

Publication number Publication date
CN101351692B (zh) 2012-11-14
US8409793B2 (en) 2013-04-02
CN101351692A (zh) 2009-01-21
FR2891052B1 (fr) 2007-12-21
US20090203066A1 (en) 2009-08-13
EP1949068A2 (fr) 2008-07-30
FR2891052A1 (fr) 2007-03-23
WO2007034071A2 (fr) 2007-03-29

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