WO2007029834A1 - Food composition - Google Patents

Abstract

Disclosed is a food composition comprising a purified extract from a migratory fish as an active ingredient. The purified extract is produced by preparing an unpurified extract from a migratory fish such as oceanic bonito belonging to the order Perciformes, the suborder Scombrina, and treating the unpurified extract using an ultrafiltration membrane having a nominal molecular weight cutoff of 5,000 or lower and/or a reverse osmosis membrane having a nominal salt-interception coefficient of 10% or lower, wherein the unpurified extract is subjected to the treatment with an adsorbent material selected from activated carton, activated earth and the like before and/or after the treatment using a membrane. In the purified extract or a fraction thereof which has the content of constituents with molecular weights of 1,000 or less of 80% or more of the total solid content of the purified extract by weight, the total bile acid content is 40 μmoles or less per 100 g of the solid content of the extract or fraction.

Description

 Description Food composition Painting field 3

 The present invention relates to a food composition, and more particularly, a food composition using a fish extract (fish extract) having anti-stress action and anti-fatigue action with reduced raw odor and bitterness. About.

 More specifically, the food composition of the present invention has an anti-stress action using an extract of migratory fish as an active ingredient, which is effective when ingested by a person who feels mental fatigue or mental stress that is felt daily. The anti-stress action referred to in the present invention means that it has either an anti-mental fatigue action that reduces or suppresses mental fatigue or an anti-stress action (in a narrow sense) that reduces stress. With regard to the present invention, such anti-psychotic fatigue action and anti-stress action (in a narrow sense) are collectively referred to as “anti-stress action (in a broad sense)” unless the meaning is different from the context.

 Furthermore, the food composition of the present invention has an anti-fatigue action comprising an extract of migratory fish as an active ingredient, which is effective when used by a person who feels fatigue on the body and fatigue after exercise. The anti-fatigue action referred to in the present invention means that it has either an anti-fatigue action that suppresses exercise fatigue or daily physical fatigue or a fatigue recovery action that promotes recovery from fatigue.

[Background]

 First, background technology regarding anti-stress action will be described.

In recent years, the involvement of stress has been suggested as the cause of various diseases and diseases. In addition, due to stress, the daily feeling of mental fatigue, emotional failure and emotional instability. Under such circumstances, the social environmental conditions that surround consumers are becoming a major problem that the environment is becoming increasingly stressful. Against this background, there is a strong demand for the development of drugs or foods and drinks having an anti-stress action that can effectively and safely prevent or reduce various disorders caused by stress.

 Examples of conventional anti-stress agents include pharmaceuticals containing tranquilizers. However, such drugs are not appropriate for many consumers who feel mental fatigue or emotional dysfunction on a daily basis because they are too strong.

 Food compositions containing cacao bean extract as food having anti-stress action (Japanese Patent Application Laid-Open No. 9-202066), Food compositions containing sweet potato extract (Japanese Patent Application Laid-Open No. 2000-013-3) 5 5 0 5) and a composition containing a pine bark extract (Japanese Patent Laid-Open No. 20 0 3-9 5 9 6 4), ganju rice cake or a food containing an extract of ganju rice cake (Japanese Patent No. 2 0 0 3-3 8 1 2 5). However, the mechanism of action of these foods has not been fully clarified, and it is difficult to say that these foods can be fully felt in humans.

 In addition, these existing foods are generally in the form of pharmaceuticals, and it was difficult to mix them with existing foods because of their sensory characteristics and physicochemical characteristics. If it is intended to be taken regularly in daily eating habits to prevent or reduce stress, it is desirable to be able to mix not only in the form of supplements but also in general foods. A dietary experience is considered preferable. From such a perspective, existing foods, pharmaceuticals, and supplement ingredients have limited applicability to food, so the development of more effective foods that are versatile as food and have a wealth of food experience. Has been desired.

On the other hand, it is known that anserine and carnosine, which are abundant in seafood and livestock meat, activate ATPase. In Japanese Patent Application Laid-Open No. 2 0 2-1 7 3 4 4 2, seafood, chicken meat and livestock meat The imidazole peptides, especially anserine, carnosine, and valenin, are specifically purified through ultrafiltration membranes from the low molecular fraction of the extract obtained from the above, and exercise ability is achieved by administration of at least one selected from these of It has been proposed that improvement and anti-fatigue effects are demonstrated. However, there is no mention of the effect of this composition on stress conditions such as mental fatigue.

 Further, in JP-A-9 2 0 6 61, a composition containing an imidazole compound such as anserine, carnosine, or valenin reduces fatigue caused by excessive mental activity in humans. It has been shown to have an anti-mental fatigue effect that enhances concentration.

 On the other hand, in Japanese Patent Publication No. 9 1 2 0 6 6 0, one or more imidazole compounds selected from the group of anserine, valenin, 7Γ-methylhistidine and monomethylhistidine, or a main component thereof is used. Anti-stress foods and drinks made by adding an extract as described above are shown. In the examples, human computational ability tests and short-term memory ability tests are conducted. However, here, an extract containing an imidazole compound as a main component is, for example, a fraction separated from a boiled juice concentrate during fish processing using ultrafiltration and ion exchange resin (Examples in the same document). 6 (see paragraph 0 0 39)), wherein the content of imidazole compound is 2% or more, preferably 10% or more, more preferably 50% or more, and particularly the content of anserine is 1% or more, It is preferably 5% or more, more preferably 20% or more (see paragraphs 0 0 14 to 0 0 15 of the same document). This composition is expensive and may not be practical for a general food. In addition, boiled juice concentrate at the time of fish processing generally has a strong raw odor and bitterness, and thus has a problem that it is difficult to take in normal food forms.

 Next, background technology regarding anti-fatigue action will be described.

The feeling of fatigue caused by exercise and physical fatigue caused by daily activities is a major problem in daily life. In general, fatigue is thought to be a signal that conveys the need for rest after excessive exercise. Sleep and rest are said to be most effective in recovering from fatigue, but fatigue that cannot be recovered by rest alone due to excessive or continuous exercise has been reported and is called chronic fatigue syndrome . Nutrition and tonic beverages that have been widely used for fatigue recovery from the past are mainly composed of various vitamins, caffeine, taurine, etc. No reversal is shown. Even if ginseng, royal jelly, or propolis protein is effective, the action point is (where the action point is the biochemical target of the active ingredient, such as a specific enzyme) ) It is not clear, and all of these products have significant sensory problems. It is difficult to use them in forms other than pharmaceutical products, and it is difficult to use them frequently in daily eating habits. -On the other hand, as explained earlier, it is known that anserine and carnosine, which are abundant in seafood and livestock meat, activate ATPase, and Japanese Patent Application Laid-Open No. 2 0 0 2— 1 7 3 4 4 In the gazette, imidazole peptides, particularly anserine, carnosine and valenin, are specifically purified through ultrafiltration membranes from low molecular fractions of extracts obtained from seafood, chicken, livestock, etc., and at least selected from these It has been proposed that one or more administrations can improve exercise ability and exert anti-fatigue effects. However, it does not show the fatigue recovery effect of the food composition of the present invention for mild exercise.

 Further, WO 2 0 0 4-0 3 2 6 5 2 reports that the fatigue after exercise is recovered by ingesting a migratory fish extract or a nutritional component having a similar composition. . However, the extract of migratory fish obtained in the invention described in this document has a strong bitter taste and raw odor, and taking the necessary intake 50 O mg (in terms of solid content) described in the invention is Because of its taste characteristics, it was difficult. Boiled juice produced during the ripening process during the production of sardines and other fish boiled fish, boiled juice produced during the ripening process during the production of salmon and other fish knots, or canned fish such as tuna and mackerel The liquid juice containing seafood extracts such as steamed juice produced during production is rich in taste ingredients, so it is processed into various extracts and seasonings after concentration.

 However, as described above, these seafood extracts are rich in taste components, but often have a raw odor and Z or bitterness, which limits their use.

To remove the raw odor of seafood extract, add sugar to seafood extract and dissolve it. A heating method (Japanese Patent Laid-Open No. 49-1010 8 2 3) is known, but in this case, there are disadvantages such as heating to cause coloring and roast odor. In addition, a method of adding a yeast extract that has been subjected to heat treatment to seafood oysters (Japanese Patent Laid-Open No. Sho 5 6-1 1 3 2 75 5), a method of inoculating and fermenting yeast or rice bran (Japanese Patent Laid-Open No. Hei 0) 8-0 2 3 9 1 7)), but none of these methods has been able to remove sufficient raw odor and bitterness.

 Therefore, the development of a more effective method for removing the bitterness and raw odor of seafood is expected.

[Disclosure of the Invention]

 [Problems to be solved by the invention]

 In the background of the background art described in the preceding paragraph, the present invention prevents, improves, reduces daily mental fatigue, mental insufficiency, and mental instability, and is ingested in a normal diet. The first object is to provide a food composition having an anti-stress action.

 Furthermore, the present invention provides a food having an anti-fatigue action, which can take a form that is easy to ingest in normal eating habits, and can prevent, reduce, or reduce the feeling of everyday fatigue and fatigue during exercise. A second object is to provide a composition.

 The present invention provides a method for removing the above-mentioned raw odor and bitterness, provides a fish extract with reduced raw odor and bitterness, and further provides a food composition using such a fish extract. The third purpose is to provide it.

[Means for solving problems]

As a result of diligent research to achieve the object described in the preceding paragraph, the present applicant has found a food composition having an anti-stress action, characterized by containing an extract of migratory fish as an active ingredient, and such a food composition. The extract of the migratory fish is extracted from (i) boiled salmon broth, (mouth) (C) After heating and concentrating boiled salmon broth and / or steamed broth, add 2-20% diatomaceous earth to the extract solids It is characterized by being an extract obtained by filtration. (2) It is an extract obtained by hot water extraction of a nodule obtained from migratory fish. ) Migratory nodules obtained from fish are subjected to enzymatic degradation with proteolytic enzymes and then extracted with hot water, or these extracts are (a) nominally Or (b) a reverse osmosis membrane having a nominal salt rejection of 10% or less, or a permeate obtained by treating with an ultrafiltration membrane having a molecular weight cut-off of 500 to 5, 000 Invented what is characterized by being a permeate obtained by treating with the above, and has already filed a patent application for this (Japanese Patent Application 2 0 0 6— 5 1 0 9 0 5).

 As a result of diligent research aimed at improving the quality of such food compositions, the present applicant has determined that an unpurified extract of migrating fish such as salmon can be used as an ultrafiltration membrane with a nominal molecular weight cut-off of 5,000 or less. Or a refined extract of migratory fish obtained by treating with a reverse osmosis membrane having a nominal salt rejection of 10% or less and subjecting it to adsorbent treatment before or after the membrane treatment; Furthermore, in such a purified extract of migratory fish, the weight of the component having a molecular weight of 1,00 or less in the solid content represents 80% or more of the total weight of the solid content, or per solid content That have a total bile acid content of 40 mo 1 e / 100 g or less have ease of drinking due to reduced raw odor and bitterness, and also have anti-stress action and anti-fatigue Based on these findings, the present invention has been completed.

That is, the present invention relates to an unpurified extract of migratory fish such as persimmon belonging to Perciformes, Scombrina, ultrafiltration membranes having a nominal molecular weight of 5,000 or less, Z and nominal Migrating by treatment with a reverse osmosis membrane having a salt rejection of 10% or less and subjecting it to an adsorbent treatment selected from activated carbon, activated clay, etc. before and / or after the membrane treatment. An anti-stress and anti-fatigue action food composition containing a purified fish extract as an active ingredient with reduced raw odor and bitterness, and such a food composition, So The weight of the component having a molecular weight of 1,000 or less in the solid content of the solid content is 80% or more of the total weight of the solid content, or the total bile acid content per solid content is 40〃mo 1 e / 10 0g or less It relates to a product that has ease of drinking due to reduced raw odor and bitterness, and also has anti-stress action and anti-fatigue action. In addition, the present invention provides that the migratory fish extract has a solid content weight of 1 in order to make it easy to ingest an effective amount of the above composition (solid extract weight of 0.5 to 1 meal). Food composition prepared to be 0.3 g or more per meal, for example, migrating fish extract, the liquid of the extract is dissolved oxygen so that it contains 0.3 g or more per meal in the solid conversion. Liquid food obtained by aseptic filling to a concentration of 5 ppm or less, with the excipient and taste-masking ingredients added so that the extract contains 0.3 g or more per serving in terms of solid content Agar, gelatin, starches, and gums so that a solid food composition obtained by pulverization, granulation, or tableting and a solid content of the migratory fish extract is 0.3 g or more per serving. Jelly-like food obtained by adding any one or more gelling agents The composition relates to such.

[Brief description of drawings]

 Fig. 1 shows changes in the content of corticosterone by various koji extracts (Experimental example 2)

 Figure 2 shows changes in G 0 T activity by various koji extracts (Experimental Example 2).

 Fig. 3 shows the amount of spontaneous locomotor activity when various sputum extracts are administered (Experimental Example 3).

 Figure 4 shows the swimming time of mice when various sputum extracts were administered (10 L / min condition) (Experimental Example 4).

 FIG. 5 shows the swimming time of mice when various sputum extracts were administered (8 L / min condition) (Experimental Example 5).

 Figure 6 shows the correlation between bile acids and bitterness and odor (Experimental Example 7).

Figure 7 shows the spontaneous locomotor activity of mice after administration of sputum extract before and after activated carbon treatment (Experimental Example 9) o [Best Mode for Carrying Out the Invention]

 Hereinafter, the present invention will be described in detail.

 The migratory fish referred to in the present invention refers to fish that moves greatly for spawning, such as salmon, salmon, swordfish, salmon, salmon, salmon, herring, and burdock, and regularly according to the season. Fish such as salmon, flounder, and flounder may be used. In particular, from the viewpoint of the effectiveness of the extract, it belongs to the Perciformes (Scombrina), such as power, mackerel, grasshopper, managa, bluefin, bluefin tuna, bluefin tuna, bigeye tuna, swordfish, etc. It is a fish. This classification is based on the “Primary Color Fish Retrieval Pictorial Book (Revised 13th Edition)” (issued in Hokuryukan 1 989). The unpurified extract of migratory fish used in the present invention is not particularly limited in its production method and can be obtained by a conventional method, but if it has the same composition, it is produced by a different production method. It may be an extract. However, from the viewpoint of production cost, boiled or steamed juices or their concentrates by-produced during the production of fishery foods such as canned migratory fish (power trout, tuna, mackerel, etc.) It is preferable to use this extract.

 In addition, such boiled juice or steamed juice or a concentrate thereof may be subjected to further concentration and purification treatment. For example, after boiled salmon meat or steamed broth is heated and concentrated to about Brix 20 to 40 by a conventional method, after adding and mixing 1 to 20% diatomaceous earth with respect to the solid content, The filtered one can be mentioned. Further, the filtrate obtained in this manner may be subjected to protease treatment, or may be added with sugar and then heated to reduce the raw odor. These are also unpurified extracts of migratory fish according to the present invention.

In addition, such an unpurified extract of migratory fish may be obtained by removing salt by electrodialysis or reverse osmosis filtration with a salt rejection of 25% or more. The method for producing the migratory fish extract of the present invention with reduced raw odor and bitterness from such an unpurified extract of migratory fish is as follows. First, for the purpose of more efficiently reducing the raw odor and bitterness of an unpurified extract of migratory fish, the nominal molecular weight cut-off is 5, 000 or less, preferably 3, 000 or less, more preferably 2, A membrane-treated permeate is obtained by treatment with an ultrafiltration membrane of 0 or less and / or a reverse osmosis membrane with a nominal salt rejection of 10% or less, preferably 5% or less. In such a permeated liquid, as a preferable characteristic from the viewpoint of antistress action and anti-fatigue action, and from the viewpoint of reducing the raw odor and bitterness, the weight of the component having a molecular weight of 1,00 or less is 80% of the total solid weight. %, Preferably 90% or more. In this case, “YM-1” (regenerated cellulose membrane) manufactured by Mi 1 1 ipore can be used in order to obtain the ratio of components having a molecular weight of 1,00 or less. Using this membrane (regardless of shape), the extract is processed to obtain a permeate. The concentrated solution is repeatedly washed with distilled water until the amount of low-molecular-weight mixture in the permeate is 1% or less of the initial permeate. That is, the low-molecular fraction present in the concentrate is removed as much as possible by water pushing, the solid content of the permeate fraction obtained in this way is measured, and the ratio of the original extract to the solid content is calculated. To do. The higher the weight ratio of the component having a molecular weight of 1,000 or less in the solid content calculated at this time, the more effective the component group having a high effect on the taste function such as umami is concentrated, It is particularly preferably 80% or more of the total weight of the extract solids. If the value is less than that, it is not preferable because the food composition of the present invention increases the amount of food consumed at one time. In order to make the ratio of the fraction with a molecular weight of 1,000 or less 80%, an ultrafiltration membrane with a molecular weight of 5,000 or less or a reverse osmosis membrane with a salt rejection of 10% or less is required. It is preferable to use and purify to some extent in advance. In the method of the present invention, either one of the film treatments may be performed, but both film treatments may be performed if necessary. In general, an ultrafiltration membrane having a molecular weight cut-off of 500 or more is known, but any membrane can be used as long as it satisfies the above conditions.

Next, in order to make this membrane-treated permeate easier to eat and drink, deodorization and decolorization treatment using an adsorbent is performed before and / or after the membrane treatment. Adsorbent used at this time Examples of the active carbon include activated carbon, activated clay, diatomaceous earth, and ion exchange resin, and it is preferable to use activated carbon from the viewpoint of cost and adsorption capacity. The activated carbon can be activated carbon of any shape, such as granular activated carbon, powdered activated carbon, granular activated carbon. The adsorbent treatment can also be performed before the film treatment.

 In addition, as another characteristic independent of the above characteristics, bile acids (Endo et al., Which are known as components involved in bitterness and raw odor of seafood extracts in migratory fish extracts)

“Nutrition and food” Vol.35, No.3, 181-187, 1982) is low. That is, the total bile acid content of migratory fish extract per solid content is not more than 40 mo 1 e / 100 g, preferably not more than 20 1mo 1 eZ 100 g, more preferably 10 ju o 1 An e / 100 g or less is an index indicating that it has ease of eating and drinking due to reduced raw odor and bitterness. The bile acid mentioned here is C _{2 4} -steroid, which is a metabolite of cholesterol, choleric acid, deoxycolic acid, chenodeoxycholic acid, lithocholic acid, ritokenocolic acid. Free bile acids such as acids, and urine conjugates of the above free bile acids such as taurocholic acid and taurodeoxycolic acid. Including. In the present specification, the total molar amount of these bile acids contained in the migratory fish extract per 100 g of migratory fish extract solids is shown as the total bile acid content. Bile acids are preferably analyzed by a method such as high performance liquid chromatography (HPLC) or gas chromatography (GLC). A method for measuring total bile acid using 3-hyperhydroxysteroid dehydrogenase is widely used for estimation of bile acid content. However, this measurement method can be applied to samples in which the type of bile acids contained in the sample is known in advance because the reactivity of the enzyme differs depending on the individual bile acids. If they are different, it is difficult to calculate the exact content, so it is preferable to quantify by the above HPLC method or GLC method.

In addition to the purified extract of migratory fish shown above, the anti-stress action or anti-fatigue of the present invention In the food composition having an action, an appropriate additive can be blended within a range not impeding the effects of the present invention. Such additives include sugars (glucose, sucrose, starch, etc.), lipids (vegetable oil, fish oil, animal fats, etc.), proteins (soybean protein) that promote the anti-stress or anti-fatigue effects of migratory fish extracts. White, milk protein, etc.), other nutrients such as minerals (potassium, sodium, calcium salts, etc.), biamines (thiamine, nisin, vitamin C, carotene, etc.); taurine, cafe Anti-fatigue ingredients such as insults; sucrose ingredients such as sucrose, aspartame, acesulfame κ, sodium glutamate, and salt suitable for imparting food function (taste, texture, safety, etc.); Granulation, pulverization, solidification of sugars and inorganic salts for the same purpose to make it easy to eat; ethyl alcohol for the same purpose, Bacteriostatic components such as acid, sodium acetate, glycine; power for similar purposes, such as pigments derived from natural products such as lamella pigment, anato pigment, benipana pigment, paprika pigment, sockeye pigment, pudou pigment, and various synthetic pigments Pigments; In addition, in order to provide the refined extract of migratory fish of the present invention as a food form that is easier to eat, for liquid foods, various extracts such as soy sauce, miso, mirin, sugar, livestock meat extract, seafood extract, vegetable extract, For powdered foods, it can also be provided by blending with seasoning materials such as powdered soy sauce, powdered miso, powdered liquor, sugar, salt, livestock extract powder, seafood extract powder, vegetable extract powder, etc. It is. Of course, other additives and food materials commonly used in this field can be blended.

 Containing an effective amount of a purified extract of migratory fish obtained by the above method (solid extract weight per meal 0.3 g or more, preferably 1 to LO g) and easy to ingest In order to make the food composition in the form, it can be blended with the above-mentioned taste-masking ingredients, other nutritional ingredients, etc. and processed as follows.

That is, when the extract is provided in the form of a liquid food composition, the migratory fish extract according to the present invention further has a solid equivalent weight of 0.3 g or more per serving. The extract solution is sterile so that the dissolved oxygen concentration is 1 O p pm or less (25 ° C). Fill to obtain the desired liquid food composition. At this time, as the container, use an aluminum voucher, an aluminum can, a steel can, a paper pack that has been subjected to deoxidation treatment on the interior, a packaging material that has oxygen scavenging properties, and a packaging material that has oxygen barrier properties. Is preferred. About liquid food composition, it can provide the composition which can distribute | circulate at normal temperature by mixing with salt, alcohol, or the foodstuff material containing these, In order to ingest this extract more effectively For reasons of taste characteristics and health reasons, it is preferable to sterilize an extract solution having a low salt content after sterilization. At this time, since the extract has a problem of flavor deterioration due to oxidation by dissolved oxygen, the dissolved oxygen concentration at 25 ° C is 5 ppm or less, preferably 3 ppm or less, and more preferably Need to be filled to 2 ppm or less.

When providing the purified extract of migratory fish of the present invention in the form of a solid food composition, the food composition contains 0.3 g or more of the purified extract of migratory fish per meal in terms of solid content. As appropriate, excipients, taste-masking ingredients, etc. are added to powder, granulate, or tablet to obtain the desired solid food composition. Excipients used at this time include carbohydrates such as glucose, sucrose, trehalose, maltose, lactose, dextrin, starch, peptides such as gelatin, casein, and partial degradation products thereof, and proteins. Can be mentioned. In addition, it is preferable to add sucrose ingredients such as sucrose, aspartame, acesulfame K, sodium glutamate, and salt to impart functions as food (taste, texture, etc.). Examples of means for powdering include spray drying, freeze drying, reduced pressure thin film drum drying, atmospheric pressure drum drying, and the like after the extract is concentrated as necessary. Further, in order to ingest the extract without being influenced by the taste, the extract can be powdered by the above-mentioned means and then filled into a capsule. Further, as a means for providing the extract in an easy-to-eat state, it is also possible to add a component having a gelling action and provide it as a jelly-like food. As the gelling agent used at this time, at least one of agar, gelatin, starches and gums is used. Also, at this time, in order to make it easier to ingest, It is preferable to add a taste ingredient such as aspartame, acesulfame K, sodium glutamate, and salt.

 The food composition of the present invention thus produced can be put on the market as it is, that is, appropriately in the form of a liquid mixture, a powder mixture or the like. In addition, it can be used to impart a favorable taste to various foods. Examples of foods include, but are not limited to, liquid food compositions and powdered food compositions such as soup, miso soup, soup, and soup shown in the Examples below.

 Finally, the dose (intake amount) of the food composition of the present invention will be described. As a result of a test in which a salmon extract (migratory fish extract) was administered to mice, an anti-stress action and an anti-fatigue action were observed when the dose exceeded 38 O mg / kg in terms of solid content. When this dose is converted from animals to humans, it will be 0.3 g or more in terms of solid content per meal as 1 to 3 meals per adult human. If the amount is below this level, the effect cannot be expected. In addition, as for the upper limit of intake, migratory fish extract such as salmon has abundant eating experience and food history, so there is no particular limitation, but an intake of 10 g per meal can provide a sufficient effect It was thought that In addition, the food composition of the present invention is mainly intended for healthy persons who feel mental fatigue on a daily basis. However, the food composition of the present invention can also be effective when ingested by persons with mental illness or depression. Is possible.

[¾W> J]

 The present invention will be further described below with reference to examples and experimental examples. 'Experimental example 1: Creation of straw extract

A koji extract was prepared by the following method. In other words, after heating and concentrating the broth of salmon meat to about Brix 30 by a conventional method, diatomaceous earth ("Radiolite # 700" manufactured by Showa Chemical Industry Co., Ltd.) is 5.0% of the extract solid content. After adding and mixing as described above, the mixture was filtered using a filter cloth, and desalted using an electrodialyzer (“Ashilyzer I” manufactured by Asahi Kasei Co., Ltd.) (鰹 extract A). The soot extract A thus obtained was subjected to ultrafiltration membrane NTU-3250 ”(manufactured by Nitto Denko Corporation; fractionation using a nominal molecular weight cut off of 6,000, and a molecular weight of over 6,000. (Fraction 11) and a fraction with a molecular weight of 6,000 or less. The obtained fraction with a molecular weight of 6,000 or less was subjected to ultrafiltration with a nominal molecular weight of 1,000 (Mi 11 ipo re Fractionation is performed using “Prep / Scale TFF6” (manufactured by Kogyo Co., Ltd.), a fraction having a molecular weight of 1,000 to 6,000 (鰹 extract C) and a fraction having a molecular weight of 1,000 or less (鰹 extract! )). Experimental example 2: Confirmation of action on restraint stress model mouse

 Two groups of 6-week old CDF-1 male mice (control group and test group) consisting of 6 animals in each group (n = 6 in each group), distilled water or sputum extract prepared in Experimental Example 1 D (solid 4.0% solution) was orally administered using a sonde so that the concentration was 2 OmL per kg of body weight. 1 hour after administration, the mouse was placed in a 2.5 cm internal diameter and 10 cm long vinyl chloride tube, imposed a restraint load of 40 to 60 minutes, and was reported as one of the stress markers in blood. The content of corticosterone and the activity of glutamic acid / oxacin-acetic acid transaminase (hereinafter referred to as GOT) were determined according to conventional methods.

The results are shown in Fig. 1 and Fig. 2 below. At this time, the same measurement was performed for mice not subjected to restraint stress loading (n = 10). In the figure, there is a significant difference at p <0.05 between the groups showing different Roman letters (different signs). That is, in Figure 1, there is no significant difference between the group showing a and the group showing b, and in Figure 2, there is no significant difference between the groups showing bc, b, or c. There is a significant difference between the groups. As shown in the figure, in the control group, it was shown that the restraint stress increased costicosterone content and GOT activity. On the other hand, the group to which sputum extract A was administered showed lower values for both corticosterone content and GOT activity than the control group. That is, it was recognized that the stress index of the stress model mouse was reduced by administration of the sputum extract. From the above results, the koji extract It was confirmed to have a stress reducing effect. In addition, when 鰹 Extract D was administered, it showed a stronger stress reducing effect (corticosterone lowering effect and GOT activity lowering effect) than other fractions (鰹 extract B or 鰹 extract C). It was speculated that the main contributing component of the anti-stress effect of Extract A was a component with a molecular weight of 1,000 or less. Experimental example 3: Fatigue recovery effect of various sputum extracts (Recovery of spontaneous momentum after exercise)

 Prepared in Experimental Example 1 after placing 5 groups of 5 male CDF-1 male mice consisting of 10 animals in each group (n = 10 in each group) on a treadmill for 3 hours. (1) Acupuncture extract A, (2) Acupuncture extract B, (3) Acupuncture extract C and (4) Acupuncture extract D are administered orally using a sonde to a solid weight of 0.86 gZkg. Then, the spontaneous momentum for 60 minutes was measured using a spontaneous momentum measuring device with an infrared sensor, and compared with the control group (Control group; only deionized distilled water was administered in the same manner as the sample). . Each sample and distilled water were administered at 0.5 mL. The results are shown in Figure 3 below. In the figure, there is a significant difference between groups showing different Roman letters (different signs) at p <0.05. That is, there is no significant difference between the group showing a and the group showing ab and between the group showing b and the group showing ab, but between the group showing a and the group showing b Are significantly different. Total counts means the number of exercises measured by the infrared sensor for 60 minutes.

As shown in Fig. 3, the fatigue recovery effect was confirmed when sputum extract A was administered. In addition, the group to which cocoon extract C (molecular weight 1,000 to 6,000 fraction) was administered exhibited spontaneous motor momentum that was almost the same as the group to which cocoon extract A was administered, and cocoon extract B (molecular weight 6, There was no significant recovery of locomotor activity in the group administered 000 or more fractions). On the other hand, the group that received sputum extract D (fraction with a molecular weight of 1,000 or less) showed higher locomotor activity than the sputum extract A administration group. From this result, it can be seen that the low molecular fraction, particularly the fraction with a molecular weight of 1,000 or less, contained in the cocoon extract A (original extract before fractionation) is enriched with a component group that has fatigue recovery action. It was suggested. Ie, cocoon extraction It was speculated that the main contributory component of the fatigue recovery action of Material A was a component with a molecular weight of 1,000 or less. Example 4: Endurance extension effect of various cocoon extracts (mouse forced swimming test; strength loading condition)

Supplied in Kyoto Omatsumoto style momentum measuring water tank (Matsumoto K., Ishihara K. ₅ Tanaka K. ₃ Inou e K., Fushiki T., J. Appl. Physiol., 81, 1843-1849 (1996)) Amount of water 1 OLZ mined mice (7-8 animals in each group, n = 7-8) were solidified with 4 types of cocoon extracts (鰹 extract AD) prepared in Experimental Example 1. It was orally administered using a sonde so that the body weight was 0.86 gZkg body weight. After 1 hour, swimming was started and the limit swimming time was measured. This limit swimming time was used as an index of anti-fatigue effect. The control group received 2 OmLZkg body weight of distilled water. Figures 4A and 4B show the swimming times of the groups administered with various sputum extracts and distilled water. In the figure, * and ** indicate significant differences from the control group, * indicates p <0. 05, and ** indicates p <0. 01.

As shown in Fig. 4A, anti-fatigue effect (endurance extension effect) was confirmed when sputum extract A was administered. In addition, as shown in Fig. 4B, the group to which sputum extract B (fraction with molecular weight of 6,000 or more) and sputum extract C (molecular weight 000 to 6,000 fraction) were administered was almost the same as the group to which distilled water was administered. As a result, it was considered that the fraction with a molecular weight of 6,000 or more and the fraction with a molecular weight of 1,000 to 6,000 had no anti-fatigue effect. On the other hand, the group to which sputum extract D (molecular weight fraction of 1,000 or less) was administered showed a longer swimming time than the sputum extract A administration group. From this result, a group of ingredients with an anti-fatigue effect (endurance extension effect) is concentrated in the low molecular weight fraction contained in strawberry extract A (original extract before fractionation), especially the fraction with a molecular weight of 1,000 or less. It has been suggested that. In other words, it was speculated that the main contributory component of the anti-fatigue effect (endurance extension effect) of soot extract A was a component having a molecular weight of 1,000 or less. Experimental example 5: Endurance extension effect of various cocoon extracts (mouse forced swimming test; mild load condition)

 Mice (10 heads in each group) acclimated to swimming in a Kyoto Daimatsumoto-type momentum measuring water tank with a water supply of 8 L / min were treated with cocoon extract A or cocoon extract D prepared in Experimental Example 1. Oral administration was performed using a sonde so that the solid content was 0.86 g / kg body weight. After 1 hour, swimming was started and the limit swimming time was measured. This limit swimming time was used as an index of anti-fatigue effect. Fig. 5 shows the swimming time of the group to which sputum extract A, sputum extract D and distilled water were administered.

 As shown in Fig. 5, the anti-fatigue effect (endurance extension effect) was confirmed when sputum extract A was administered even under the condition of 8 L / min of water supply. In addition, the group that received sputum extract D (fraction with a molecular weight of 1,00 or less) showed a longer swimming time than the sputum extract A administration group even under this condition. From this result, it is shown that the component with anti-fatigue effect (endurance extension effect) in the low molecular weight fraction contained in strawberry extract A (original extract before fractionation), especially the fraction with a molecular weight of 1,00 or less It was suggested that the group was enriched. In other words, it was speculated that the main contributing component of the anti-fatigue effect (endurance extension effect) of cocoon extract A is a component with a molecular weight of 1,00 or less. Experimental Example 6: Removal of raw odor and bitterness by activated charcoal treatment of persimmon extract

 The persimmon extract A prepared in Experimental Example 1 had a strong odor and bitterness, and as it was, it was difficult to take orally. On the other hand, for cocoon extract A, cocoon extract D (molecular weight of 1,000 or less fraction) obtained by fractionation under the conditions of fractional molecular weight of 1,00 or less Compared with A, the raw odor and bitterness are somewhat weak, but it is still difficult to ingest as it is.

表 1に示したように、 鰹抽出物 A (元のエキス） 、 鰹抽出物 E (分子量 6, 0 00以下） および鰹抽出物 D (分子量 1, 000以下） のいずれの抽出物におい ても、 活性炭処理を行うことにより、 生臭みおよび苦味が減少することが示され た。 また、 添加した活性炭量が多くなるにつれて、 生臭みおよび苦味が弱くなる ことが示された。 さらに、 鰹抽出物 A (元のエキス） および鰹抽出物 E (分子量 6, 000以下） について洁性炭処理を行って得られた試料に比べて、 鰹抽出物 D (分子量 1, 000以下） について同等の条件にて活性炭処理を行って得られ た試料のほうが、 生臭みおよび苦味が弱い傾向が確認された。 すなわち、 表 1に 示すように、 鰹抽出物 A (元のエキス） および鰹抽出物 E (分子量 6, 000以 下） について活性炭 1. 0%で処理して得られた抽出物の評点が + +であったの に対して鰹抽出物 D (分子量 1, 000) について活性炭 1. 0%で処理して得 られた抽出物の評点は十であった。 この結果から、 限外濾過処理と活性炭処理と を組み合わせることにより、 効率よく生臭みおよび苦味を除去することが可能で あることが示された。 実験例 7 ：各種鰹抽出物中の胆汁酸含量の分析 Therefore, in order to solve this problem, the molecular weight of cocoon extract obtained by fractionating cocoon extract A, cocoon extract D, and cocoon extract A with molecular weight 6, 00 0 Deodorizing treatment using activated charcoal was tried for the fractions less than or equal to 6,00 0 (soot extract E).鰹 Extract A, 鰹 Extract D and 鰹 Extract E, respectively, solid content concentration 5. Dissolve or dilute to 0%. Activated charcoal (“activated carbon V 6”, Hokuetsu Carbon Co. Was added for 1 hour at 45 ° C., and the stirring operation was carried out using a swirler and a stirrer. After stirring, the mixture was filtered using “Toyo No. 2 filter paper” (manufactured by Advantech Toyo Co., Ltd.) to obtain an activated carbon treated soot extract. The obtained activated charcoal-treated extract was subjected to sensory evaluation by five taste panels. About raw smell and bitterness, one (not feel at all), + (feel slightly), ++ (feel weak), + + + (feel), + + + + (feel strong), and + + + + + A 6-point evaluation (feeling very strong) was performed. The obtained evaluation results are shown in Table 1 below. Table 1. Various candy :! : Changes in raw odor and bitterness due to activated carbon treatment of kiss

As shown in Table 1, in any of the extracts of strawberry extract A (original extract), cocoon extract E (molecular weight of 6,000 or less) and strawberry extract D (molecular weight of 1,000 or less) It was shown that the live smell and bitterness decreased by the activated carbon treatment. It was also shown that the raw odor and bitterness became weaker as the amount of activated carbon added increased. In addition, cocoon extract D (molecular weight 1,000 or less) compared to the sample obtained from the cocoon extract A (original extract) and cocoon extract E (molecular weight 6,000 or less). It was confirmed that the samples obtained by performing the activated carbon treatment under the same conditions for were less odorous and bitter. That is, as shown in Table 1, the extract obtained by treating 1.0% of activated charcoal extract A (original extract) and strawberry extract E (molecular weight 6,000 or less) with a score of + In contrast, the extract obtained by treating 1.0% of activated charcoal extract D (molecular weight 1,000) with 10% activated charcoal had a score of ten. From this result, ultrafiltration treatment and activated carbon treatment It was shown that it was possible to remove the raw odor and bitterness efficiently by combining the two. Example 7: Analysis of bile acid content in various koji extracts

 Bile acids have been identified as components that exhibit the bitter taste and raw smell of seafood extracts (Endo et al., “Nutrition and Food” VoL 35, No. 3, 181-187, 1982). Bile acids were analyzed for the purpose of confirming whether or not the raw odor and bitterness of the sample obtained by treatment with activated charcoal in Experiment 6 were reduced. The analysis of bile acids was performed by separating the bile acids by HPLC and then reacting them through an immobilized enzyme column (3-hydroxysteroid dehydrogenase column) (Okuyama, Clinical Pathology Vo 1.29, No. 5). , 446-458, 1981). The analysis results obtained are shown in Table 2 below.

Table 2. Change in bile acid content by activated charcoal treatment of various cocoon I kisses

 (Solid content 100g, // mole)

As shown in Table 2, extraction of cocoon extract A '(original extract), cocoon extract E (molecular weight 6, 000 0 or less) and cocoon extract D (molecular weight 1, 000 0 or less) It was also shown that the bile acid content (mo 1 e per 100 g of solid content) decreased by treatment with activated carbon. Also, as the amount of added activated carbon increased, the bile acid content was found to show a low value. In addition, cocoon extract D (molecular weight 1, 0 0 0) was compared to the sample obtained by treating activated carbon for cocoon extract A (original extract) and cocoon extract E (molecular weight 6,00 0 or less). 0 or less) was confirmed to have a lower bile acid content in samples obtained by treatment with activated carbon under the same conditions. It was. From these results, it was shown that bile acids, which are raw odor and bitter components, can be efficiently removed by combining ultrafiltration treatment and activated carbon treatment.

 In addition, a correlation analysis was made between the bile acid content of each sample, that is, various commercially available koji extracts or each koji extract obtained in Experimental Example 3 (16 points in total) and the odor and bitterness intensity (Spearman's Rank correlation analysis). The results are shown in Figure 6 below.

 As shown in Figure 6, a positive correlation (correlation coefficient / 0 = 0. 968, risk factor p <0.001) was observed between the bile acid content and the score of raw odor and bitterness. . This result suggests that bile acids contribute significantly to the raw odor and bitterness in koji extract, and the possibility that bile acid content in koji extract can be used as an indicator of extract quality was shown. . In addition, the raw odor and bitterness of a 5% solid content solution of a strawberry extract with a bile acid content of 40/1110 16/100 or less in the solid content is “slightly felt”, so it is easy to eat, and As a condition of a koji extract having an anti-stress action and an anti-fatigue action, the bile acid content per extract solid content is preferably 40 mole / 100 g or less. Furthermore, since the raw odor and bitterness of a 5% solids solution of an extract with a bile acid content of 10 Π 1 Ο 1 e / 100 g or less are `` not felt at all '', it is easy to eat, has an anti-stress effect, and As a condition of the koji extract having anti-fatigue action, it is considered most preferable that the bile acid content per solid extract is 10 gm 01 eZ 100 g or less.

Incidentally, boiled acid content and sensory evaluation (evaluation with a 5% solid content solution) were performed on a commercially available koji extract (boiled koji soup concentrate). The results are shown in Table 3 below. Table 3. Bile acid content in various commercial salmon I kisses

(Included 100g, jtimole) As shown in Table 3, each of the sachet extracts had a strong raw odor and a bitter taste, and the bile acid content per solid content also showed a value of 200 zmo 1 eZl 00 g or more. Example 8: Measurement of anti-stress effect of cocoon extract treated with activated carbon

 Three groups of 5 week-old CDF-1 male mice consisting of 5 animals in each group (n = 5 in each group), respectively, distilled water, and potato extract D prepared in Experimental Example 1 (solid content 4. 0% solution) and potato extract D 2% activated carbon sample obtained by treatment with 2% activated carbon (solid solution 4.0% solution) was orally administered using a sonde so that it would be 2 OmL per kg body weight. Administered. One hour after administration, the mouse was placed in a 2.5 cm ID and 10 cm length salt-vinyl chloride tube, imposed a restraint load of 40-60 minutes, and was reported as one of the stress markers in blood. The content of corticosterone was measured according to a conventional method. As a result, there was no significant difference in corticosterone content between the group administered with cocoon extract D and the group administered with the activated carbon-treated sample of cocoon extract D. From these results, it was shown that the anti-stress effect was not reduced by the activated carbon treatment, and that it was easy to eat and had a high anti-stress effect. Experimental Example 9: Measurement of fatigue recovery effect of soot extract treated with activated carbon

5-week-old CDF-1 male mice consisting of 10 to 11 animals in each group (each group n = 10 to l 1) 5 groups were placed on a treadmill and forced walking exercise was applied for 3 hours. 1) The same soot extract D as in Experiment 1 and (2) The extract (salt extract F) obtained by subjecting soot extract D to activated charcoal (AC) was converted to 0.86 g in terms of solid weight. Oral administration using a sonde so that the body weight becomes / kg body weight, and then, the spontaneous exercise amount is measured for 60 minutes using a spontaneous exercise amount measuring device with an infrared sensor, and the control group (Con tro 1 group; deionized distilled water only sample) And administration in the same manner as above. Each sample and distilled water were administered at 0.5 mL. The results are shown in Figure 7 below. As shown in FIG. 7, the fatigue recovery effect was confirmed when sputum extract D was administered. In addition, the group to which sputum extract F was administered showed spontaneous motor activity almost equal to that of the sputum extract D administration group. From this result, it was shown that by performing activated carbon treatment on a fraction having a molecular weight of 1,000 or less, it is possible to prepare a koji extract that has a strong fatigue recovery action and is easy to eat. Experimental Example 10: Ratio and taste characteristics of fractions with molecular weight of 1,000 or less of various koji extracts (koji extract)

About the same cocoon extract A (original extract), cocoon extract E (molecular weight of 6,000 or less) and cocoon extract D (molecular weight of 1,000 or less) (both 5% solid solution) Then, “activated carbon V 6” was added to 2% of the liquid volume, mixed and stirred, and then filtered using filter paper “Toyo No. 2” to obtain various activated carbon treated soot extracts. In addition, 鰹 Extract A was measured according to the ultrafiltration membrane “Milipore“ PM-5 ”(fractionated molecular weight 5,000)” or Asahi Kasei “Microza UF SEP — 1013” (fractionated molecular weight 3,000). For the permeate (5% solid content) obtained by fractionation, add “activated carbon V6” to 2% of the liquid volume, mix and stir, then use filter paper “Toyo No. 2”. Filtration was performed to obtain an activated carbon treatment product. These obtained extracts were subjected to sensory evaluation by five taste screening panels. About raw smell and bitterness, one (not feeling at all), + (feeling slightly), ++ (feeling weak), +++ (feeling), +++++ (feeling strong), and +++++ A 6-point evaluation of + (I feel very strong) was performed. Further, the solid extract recovery rate of the permeate was measured for the obtained extract using “¥ 1 ^ -1 membrane” of ^ 11111 0] ^ 6. The results are shown in Table 4 below. W

 Table 4.Molecular weight ratio of various koji extracts and the intensity of bitterness and raw odor

表 4に示したように、 鰹抽出物 A (元エキス） および鰹抽出物 E (分子量 6, 000以下） について活性炭処理を行って得られたエキスについては、 わずかに 苦味および生臭みを有していた。 一方、 鰹抽出物 D (分子量 1, 000以下）、 分子量 3, 000以下の画分 （いずれも固形分 5%溶液） および分子量 5， 00 0以下の画分について活性炭処理を行って得られたエキスはほとんど苦味および 生臭みを有していなかった。 これらのエキスの分子量 1， 000以下画分の比率 が 80%以上であったことから、 上述の方法にて苦味および生臭みを低減するた めには、 分子量 1, 000以下画分の比率が 80%以上であることが好ましいと 考えられた。 実施例 1 ：抗ストレス作用および抗疲労作用を有する粉末状食品組成物の調製 （ その 1)

As shown in Table 4, the extract obtained from the activated carbon treatment of cocoon extract A (original extract) and cocoon extract E (molecular weight 6,000 or less) has a slight bitter taste and raw odor. It was. On the other hand, cocoon extract D (molecular weight of 1,000 or less), molecular weight of 3,000 or less (both solid 5% solution) and molecular weight of 5,000 or less were obtained by activated carbon treatment. The extract had almost no bitterness and odor. Since the ratio of the molecular weight fraction of these extracts was 80% or more, the fraction of the molecular weight of 1,000 or less was used in order to reduce bitterness and raw odor by the method described above. 80% or more was considered preferable. Example 1: Preparation of a powdered food composition having anti-stress and anti-fatigue effects (Part 1)

 After concentrating the boiled soup produced as a by-product during the production of bonito under reduced pressure to about Bri X 30 using a conventional method, diatomaceous earth ("Radiolite # 700" manufactured by Showa Chemical Industry Co., Ltd.) is reduced to 5.0 After adding and mixing so that the content of the solution is 1%, it is filtered using a filter cloth and desalted using an electrodialyzer ("Asalyzer 1" manufactured by Asahi Kasei Co., Ltd.). Extract).

Using 20 kg of this desalted koji extract (solid content 10%) using ultrafiltration membrane “Prep / Scale TFF6” (Mil lipore, nominal molecular weight cut off 1,000) And fractionated to obtain a fraction having a molecular weight of 1,000 or less (solid content: 6.0%) 18.0 kg. To this fraction solution, add "activated carbon V6" to a molecular weight of 1,000 or less so that the volume of the fraction solution is 2.4%, and stir at 45 ° C for 1 hour. The extract was subjected to pressure filtration (“Toyo No. 2”) to obtain an activated carbon-treated extract. With respect to the extract thus obtained, the solid content recovery rate of the permeate was measured using Millipore "YM-1 membrane" and found to be 98.0%. Furthermore, as a result of quantifying the bile acid contained in the extract, it was 6.0 mo 1 e (taurocolic acid 6.0 mol) per 100 g of the solid content.

 After concentrating this extract to 20% solids by vacuum concentration, add dextrin (“Sandeck # 100”, manufactured by Yuwa Starch Kogyo Co., Ltd.) with the same weight as the extract solids, dissolve and spray dry. Powdering was performed to obtain 0.9 kg of a powdery food composition having an anti-stress action and an anti-fatigue action. Example 2: Preparation of a powdery food composition having anti-stress and anti-fatigue effects (Part 2)

Boiled boiled soup produced as a by-product during the production of bonito is heated and concentrated to about Bri x 30 by a conventional method, and then diatomaceous earth (“Radiolite # 700” manufactured by Showa Chemical Industry Co., Ltd.) is reduced to 5.0% of the solid extract After adding and mixing as such, filtration was performed using a filter cloth. 50 kg of this unrefined koji extract (25% solids) was added with 2.5 kg of “activated carbon V6”, stirred at 50 ° C. for 1.5 hours, and filtered using a Phil evening press. An activated carbon treated extract was obtained. The activated charcoal-treated extract thus obtained was fractionated using an ultrafiltration membrane “Pr epZScal e TFF6” (Mi 11 ipore, nominal molecular weight cut off 1,000), and molecular weight less than 1,000. Fraction (solid content 12.0%) 45.0 kg was obtained. With respect to the extract thus obtained, the solid content recovery rate of the permeate was measured using “YM-1 membrane” manufactured by Mi 11 ipore, and found to be 96.8%. Furthermore, as a result of quantifying the bile acids contained in the above extract, 1.4 moles per 100 g of solid content (taurocholic acid 1.4 / mole) Met.

 After concentrating this extract to 20% solids by vacuum concentration, add dextrin (“Sandeck # 100”, ≡ product made by Japanese Starch Kogyo Co., Ltd.) of the same weight as the extract solids, dissolve and spray dry. Powdered rice cake was applied to obtain 2.5 kg of a powdered food composition having antistress and anti-fatigue effects. Example 3: Preparation of a powdered food composition having anti-stress and anti-fatigue effects (Part 3)

 After heating and concentrating boiled soup that was produced as a by-product during the production of bonito to about Brix 30 diatomaceous earth (“Radiolite # 700” manufactured by Showa Chemical Industry Co., Ltd.) 5.0% After adding and mixing, the mixture was filtered using a filter cloth. Add 2.5 kg of “activated carbon V6” to 50 kg of the obtained unripe koji extract (solid content 25%), stir at 50 ° C for 1.5 hours, and filter using a filter. An activated carbon-treated extract was obtained. The activated charcoal-treated extract thus obtained was fractionated using an ultrafiltration membrane “ROMICON PM-1” (manufactured by Komatsugawa Kako Co., Ltd., nominal molecular weight cut-off 1, 00), and a fraction having a molecular weight of 1,000 or less. 44 to 0 kg was obtained (solid content 12.5%). With respect to the extract thus obtained, the solid content recovery rate of the permeate was measured using “YM-1 membrane” manufactured by Millipore Corporation, and found to be 95.0%. Furthermore, as a result of quantifying the bile acid contained in the above extract, it was 4.5 mo 1 e (taurocholic acid 4.5 mole) per 100 g of solid content.

Concentrate this extract under reduced pressure to a solid content of 20%, add dextrin (“Sandake # 100”, Sanwa Starch Kogyo Co., Ltd.) with the same weight as the solid extract, dissolve and spray dry. The powder was pulverized to obtain 5.2 kg of a powdered food composition having anti-stress action and anti-fatigue action. Example 4: Preparation of powdered food composition having anti-stress action and anti-fatigue action (Part 4)

 After heating and concentrating boiled soup produced as a by-product during the production of bonito to about 30 Bri X, diatomaceous earth (“Radiolite # 700” manufactured by Showa Chemical Industry Co., Ltd.) is 5.0% of the solid extract After adding and mixing so that it became, it filtered using the filter cloth. Add 2.5 kg of “activated carbon V6” to 50 kg of unrefined koji extract (solid content 25%) of this filtrate. The mixture was stirred at C for 1.5 hours and filtered using a filter press to obtain an activated carbon-treated extract. The activated charcoal-treated extract thus obtained was fractionated using an ultrafiltration membrane “Microza UFSEP-1013” (manufactured by Asahi Kasei Co., Ltd., nominal molecular weight 3,000) and having a molecular weight of 3,000 or less. Fraction (solid 15.2%) 45.0 kg was obtained. With respect to the extract thus obtained, the solid content recovery rate of the permeate was measured using Mi 11 ipor “YM-1 membrane” and found to be 82.4%. Furthermore, as a result of quantifying the bile acid contained in the extract, it was 8.2 zmo 1 e (taurocholic acid 8.2 zmole) per 100 g of solid content.

 Concentrate this extract under reduced pressure to a solid content of 20%, add dextrin (“Sandake # 100”, Sanwa Starch Kogyo Co., Ltd.) with the same weight as the solid extract, dissolve and spray dry. A powdery food composition having anti-stress action and anti-fatigue action was obtained, and 5.6 kg was obtained. Example 5: Preparation of persimmon extract with reduced bitterness and odor

 After concentrating the boiled broth produced as a by-product during the production of bonito into vacuum to approximately 30 Bri X, diatomaceous earth ("Radiolite # 700" manufactured by Showa Chemical Industry Co., Ltd.) is added to the solid content of 5.0. After adding and mixing so that the content of the solution is 1%, it is filtered using a filter cloth and desalted using an electrodialyzer ("Asalyzer 1" manufactured by Asahi Kasei Co., Ltd.). Extract).

This desalted koji extract (solid content 10%) 20 kg was fractionated using reverse osmosis membrane “NTR-74 10” (Nitto Denko Corporation, nominal salt rejection 5%), and extracted (solid content 5. 0%) 17.2 kg was obtained. To this fraction solution, add “activated carbon V6” to 2.0% of the volume of the extract solution, stir at 45 ° C for 1 hour, and filter under reduced pressure (“Toyo No 2)) to obtain an activated carbon-treated extract. For the extract obtained in this way, measure the solids recovery rate of the permeate using Mi 11 ipore "YM-1 membrane". As a result, it was 99.2%. Furthermore, as a result of quantifying the bile acid contained in the extract, it was 3.0 mo 1 e (taurocholic acid 3.0 mo 1 e) per 100 g of solid content.

 Concentrate this extract under reduced pressure to a solid content of 20%, add dextrin (“Sandake # 100”, Sanwa Starch Kogyo Co., Ltd.) with the same weight as the solid extract, dissolve and spray dry. Powdered fish extract 1. 2 kg was obtained.

Example 6: Preparation of liquid packaged food of koji extract (liquid food composition)

 According to the preparation method of Experimental Example 1, a fraction with a molecular weight of 1,000 or less of koji extract was prepared. To the resulting extract (solid content: 5.0%), 1% of activated carbon V 6 was added, and the mixture was stirred at 45 ° C for 1 hour, and then filtered using a filter evening press. To obtain an activated charcoal-treated extract. The extract was purged with nitrogen and then sterilized by heating at 130 ° C for 60 seconds. After that, 100 mL was filled in a paper pack container that was internally coated with aluminum. The dissolved oxygen concentration at this time was 1.2 p pm. The solid content of the koji extract in the container was 5.0. Example 7: Preparation of koji extract powder (powdered food composition)

After concentrating the activated charcoal-treated rice bran extract prepared in Example 1 to a solid content of 30% and adding and dissolving dextrin (Sanwa Starch Co., Ltd. “Sandeck # 100”) of the same weight as the solid extract, Drying was performed by spray drying to obtain a koji extract powder. 3g of this powder was filled into an aluminum pouch and a packaged product containing 1.5g of potato extract solids per serving (1 bag) was obtained. Example 8: Preparation of koji extract granules (granular food composition)

 Aspartame (manufactured by Ajinomoto Co., Inc.) 0.5 part by weight, sodium glutamate (“MSG-RC” manufactured by Ajinomoto Co., Inc.) 10 parts by weight with respect to 100 parts by weight of the koji extract powder prepared in Example 2 After adding and mixing parts by weight and 3.5 parts by weight of brine, granulation was performed using an extrusion granulator to obtain soot extract granules. 3 g of this granule was filled in an aluminum pouch to obtain a packaged product containing 1.43 g of solid content of persimmon extract per serving (1 bag). Example 9: Preparation of strawberry extract tablets (tablet food composition)

 Aspartame (Ajinomoto Co., Inc.) 0.5 parts by weight and sodium glutamate (Ajinomoto Co., Ltd. “MSG-FC”) 5 parts by weight with respect to 100 parts by weight of the koji extract powder prepared in Example 3 After adding and mixing the parts, tableting was performed using a tableting machine to obtain 2.2 gZ tablets containing the koji extract as an active ingredient. The weight of the koji extract solid per tablet was 1.05. Example 1 0: Preparation of jelly-like cocoon extract food (jelly-like food composition)

 Liquid of koji extract obtained in Example 2 (permeate of ultrafiltration treatment, solid content 12.0%) 50 parts by weight, water 150 parts by weight, aspartame (manufactured by Ajinomoto Co.) To 2 parts by weight and 2.0 parts by weight of sodium glutamate, 4.0 parts by weight of gelatin (manufactured by Nitta Gelatin Co., Ltd.) was added, dissolved by heating, and then filled in 100 g of an aluminum container, then at 10 ° C. 1 5 minutes sterilization under pressure and cooling to obtain a jelly-like koji extract food. The weight of solid content of persimmon extract contained in each food composition was 2.1 g. Example 1 1: Preparation of jelly-like koji extract food (jelly-like food composition)

Liquid of koji extract obtained in Example 2 100 parts by weight, water 100 parts by weight, aspartame (manufactured by Ajinomoto Co.) 0.2 parts by weight and sodium glutamate 2.0 parts by weight Add 0.5 parts by weight of gelatin (made by Nitta Gelatin Co., Ltd.), heat-dissolve, fill 100 g in an aluminum container, perform autoclaving at 120 ° C for 15 minutes, cool, A jelly-like koji extract food was obtained. The weight of the solid extract of persimmon extract contained in this food composition was 2.42. Example 12: Preparation of soup-like food composition (liquid food composition)

 Liquid of koji extract obtained in Example 2 (permeate of ultrafiltration treatment, solid content: 12.0%) 10 kg, concentrated soy sauce 6 kg, and sodium glutamate (“MSG-RC” manufactured by Ajinomoto Co., Inc.) 0 1kg was mixed and dissolved. The liquid thus obtained was heat sterilized at 120 ° C. for 60 seconds, then purged with nitrogen, and aseptically filled into aluminum pouches every 40 g, to obtain the liquid packaged food of the present invention. The weight of solid content of persimmon extract per serving (1 bag) of the obtained food was 2.98 g. The dissolved oxygen concentration of this liquid composition was 0.9 ppm. Example 13: Preparation of a powdered miso soup-like food composition (powdered food composition)

 Powdered miso soup-like food powder was obtained by mixing 6.0 kg of the koji extract powder obtained in Example 2 and 7.5 kg of powdered miso (manufactured by Hanamaruki). Each 13.5g of this powder was filled into an aluminum pouch to obtain a powdered miso soup-like packaged food. The weight of the koji extract solids contained in one serving of this composition was 3.0. Example 14: Preparation of powder soup-like food composition (powdered food composition)

Powder of koji extract obtained in Example 3 1.0 kg, powdered soy sauce (manufactured by Yamasa Co., Ltd.) 2.4 ¾: crushed and mixed 0.7 kg of salt, 0.3 kg of granulated sugar and 0.2 kg of powdered sake A powdered soup-like food was obtained. The powder thus obtained was filled in 23 g each in an aluminum pouch to obtain a packaged powder soup-like food composition. The weight of the solid extract of cocoon extract contained in one meal (1 bag) of this composition was 2.5 g. Example 15: Preparation of a powdered soup-like food composition (powdered food composition)

 Koji extract powder obtained in Example 2 1.0 kg, onion extract powder (manufactured by Nikken Foods) 0.2 kg, cabbage extract powder 0.1] £, salt 0.5 kg, white paper powder Powdered soup-like food was obtained by grinding 0.02 kg, sodium glutamate (FC) 0.3 kg, granulated sugar 0.3 kg and sodium inosinate (TRI) 0.03 kg. 7.0 g of the powder thus obtained was filled in an aluminum pouch bag to obtain a packaged powder soup-like food composition. In addition, the weight of the solid content of the koji extract contained in one meal (one bag) of this composition was 1.5 g.

[Industrial applicability]

 According to the present invention, it is possible to easily provide a food composition containing a fish extract with reduced raw odor and bitterness, and further containing it.

 Food composition with anti-stress action that can prevent or reduce stress-induced conditions such as mental fatigue, mental fatigue or mental failure, or mental instability Things can be provided easily.

 Furthermore, it is possible to easily provide a food composition having an anti-fatigue action capable of preventing and reducing fatigue feelings such as fatigue feeling felt daily and fatigue feeling after exercise.

Claims

O 2007/029834 Claims 1. Unpurified extract of migratory fish belonging to Perciformes (Scibrina) or ultrafiltration membranes with a nominal molecular weight of 5,000 or less or reverse osmosis membranes with Z and a nominal salt inhibition rate of 10% or less And a purified extract of migratory fish obtained by subjecting to an adsorbent treatment before or after the membrane treatment, and after the membrane treatment, as an active ingredient.  2. The food composition according to claim 1, wherein the weight of the constituent component having a molecular weight of 1,000 or less in the solid content of the purified extract of the migratory fish is 80% or more of the total weight of the solid content. .  3. The food composition according to claim 1 or 2, wherein the purified extract of the migratory fish has a total bile acid content per extract solid content of 40 mo 1 e / 100 g or less.  4. The food composition according to any one of claims 1 to 3, wherein the migratory fish is salmon.  5. The food composition according to any one of claims 1 to 4, wherein the adsorbent is activated carbon, activated clay, or diatomaceous earth.  6. The food composition according to any one of claims 1 to 5, wherein the crude extract of the migratory fish is an extract obtained by hot water extraction of the migratory fish.  7. The food composition according to claim 6, wherein the unpurified extract of migratory fish is boiled or steamed juice obtained at the time of manufacturing bonito or canned salmon, or a concentrate thereof.  8. The food composition according to any one of claims 1 to 7, wherein the purified extract of migratory fish as the active ingredient is contained in an amount of 0.3 to 10 g per serving in terms of solid content. 9. Dissolved oxygen concentration of 5ppm or less (25 ° C) in the extract so that it contains 0.3 to 10g per serving of the purified extract of migratory fish, which is the active ingredient. Aseptic filling is performed so as to become any one of claims 1 to 8 The liquid food composition as described.  10. The refined extract of migratory fish, which is the active ingredient, is powdered by adding an excipient and a taste-masking ingredient so as to contain 0.3 to 0 L per serving in terms of solid content. The powdered food composition according to any one of claims 1 to 8, wherein the composition is obtained.  11. The refined extract of migratory fish, which is the active ingredient, is added with an excipient and a taste-masking ingredient so as to contain 0.3 g: L 0 g per serving in terms of solid content. The granular food composition according to any one of claims 1 to 8, which is obtained by dripping.  12. Add the excipient and taste-masking ingredient so that the refined extract of the migratory fish, which is the active ingredient, contains 0.3 to L; The tablet-like food composition according to any one of claims 1 to 8, wherein the tablet-like food composition is obtained.  13. Any one of agar, gelatin, starch or gum so that the refined extract of migratory fish, which is the active ingredient, contains 0.3 to 1 Og per serving in terms of solid content. Claims obtained by adding more than one kind of gelling agent! The gel-like food composition according to any one of 8 to V.  14. The food composition according to claim 1, further comprising at least one of soy sauce, powdered soy sauce, miso, powdered miso, vegetable extract and vegetable extract powder. object.  15. The food composition according to any one of claims 1 to 14, wherein the food composition is a food composition having an anti-fatigue action.  16. The food composition according to any one of claims 1 to 14, wherein the food composition is a food composition having an anti-stress action.  17. The food composition according to any one of claims 1 to 14, wherein the food composition is a fish extract with reduced raw odor and bitterness.