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WO2007024840A3 - Methode de quantification d'acides nucleiques - Google Patents

Methode de quantification d'acides nucleiques Download PDF

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Publication number
WO2007024840A3
WO2007024840A3 PCT/US2006/032735 US2006032735W WO2007024840A3 WO 2007024840 A3 WO2007024840 A3 WO 2007024840A3 US 2006032735 W US2006032735 W US 2006032735W WO 2007024840 A3 WO2007024840 A3 WO 2007024840A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
subset
target nucleic
microparticles
flow cytometry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/032735
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English (en)
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WO2007024840A2 (fr
Inventor
Christopher D Tunkey
Theresa L O'keefe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chiesi USA Inc
Original Assignee
Critical Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Critical Therapeutics Inc filed Critical Critical Therapeutics Inc
Publication of WO2007024840A2 publication Critical patent/WO2007024840A2/fr
Publication of WO2007024840A3 publication Critical patent/WO2007024840A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une méthode destinée à mesurer une espèce d'acide nucléique cible dans un échantillon (par ex., un échantillon biologique), et consistant à amplifier de multiples concentrations connues d'un acide nucléique standard, cet acide nucléique standard comprenant la même séquence que l'acide nucléique cible ou un fragment correspondant, à amplifier l'acide nucléique cible, à hybrider l'acide nucléique cible amplifié avec une population comprenant un sous-ensemble de microparticules en vue de la formation d'un mélange, chaque sous-ensemble pouvant être distingué des autres sous-ensembles sur la base d'un paramètre détectable, un sous-ensemble de microparticules étant couplé à un oligonucléotide complémentaire d'une partie des acides nucléiques cible et standard, à hybrider chacun des échantillons d'acide nucléique standard amplifié avec une population comprenant ledit sous-ensemble de microparticules en vue de la formation de mélanges, à analyser les mélanges par cytométrie de flux, la liaison d'un acide nucléique cible et d'échantillons d'acide nucléique standard à un sous-ensemble de microparticules étant mesurée sur la base de la détection d'un marqueur, et à déterminer la quantité d'acide nucléique cible dans l'échantillon biologique.
PCT/US2006/032735 2005-08-22 2006-08-22 Methode de quantification d'acides nucleiques Ceased WO2007024840A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71055805P 2005-08-22 2005-08-22
US60/710,558 2005-08-22

Publications (2)

Publication Number Publication Date
WO2007024840A2 WO2007024840A2 (fr) 2007-03-01
WO2007024840A3 true WO2007024840A3 (fr) 2007-04-12

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Family Applications (1)

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PCT/US2006/032735 Ceased WO2007024840A2 (fr) 2005-08-22 2006-08-22 Methode de quantification d'acides nucleiques

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WO (1) WO2007024840A2 (fr)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
WO2009111072A1 (fr) * 2008-03-05 2009-09-11 Clean Earth Technologies, Llc Dosage par cytométrie en flux avec des billes à marquage pna et avec couplage pcr multiplexé pour la détection simultanée de plusieurs agents biologiques
WO2010033200A2 (fr) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Création de bibliothèques de gouttelettes et d'espèces apparentées
EP3150724A1 (fr) 2008-12-19 2017-04-05 President and Fellows of Harvard College Séquençage d'acide nucléique assisté par particules
AU2010315580B2 (en) 2009-10-27 2014-11-06 President And Fellows Of Harvard College Droplet creation techniques
WO2013188756A1 (fr) 2012-06-15 2013-12-19 The University Of Chicago Immuno-essais multiplexés quantitatifs faisant intervenir des oligonucléotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2881685C (fr) 2012-08-14 2023-12-05 10X Genomics, Inc. Compositions de microcapsule et procedes
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2931919B1 (fr) 2012-12-14 2019-02-20 10X Genomics, Inc. Procédés et systèmes pour le traitement de polynucléotides
JP2016511243A (ja) 2013-02-08 2016-04-14 テンエックス・ジェノミクス・インコーポレイテッド ポリヌクレオチドバーコード生成
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US20160279068A1 (en) 2013-11-08 2016-09-29 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CA2943624A1 (fr) 2014-04-10 2015-10-15 10X Genomics, Inc. Dispositifs fluidiques, systemes et procedes permettant d'encapsuler et de separer des reactifs, et leurs applications
EP3161162A4 (fr) 2014-06-26 2018-01-10 10X Genomics, Inc. Analyse de séquences d'acides nucléiques
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
JP2017526046A (ja) 2014-06-26 2017-09-07 １０エックス ゲノミクス，インコーポレイテッド 核酸配列アセンブルのプロセス及びシステム
WO2015200893A2 (fr) 2014-06-26 2015-12-30 10X Genomics, Inc. Procédés d'analyse d'acides nucléiques provenant de cellules individuelles ou de populations de cellules
CN107002128A (zh) 2014-10-29 2017-08-01 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
EP3244992B1 (fr) 2015-01-12 2023-03-08 10X Genomics, Inc. Procédés de codage a barres d'acides nucléiques
SG11201705425SA (en) 2015-01-13 2017-08-30 10X Genomics Inc Systems and methods for visualizing structural variation and phasing information
EP3256606B1 (fr) 2015-02-09 2019-05-22 10X Genomics, Inc. Systèmes et procédés pour déterminer la variation structurale
US11274343B2 (en) 2015-02-24 2022-03-15 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequence coverage
EP3262407B1 (fr) 2015-02-24 2023-08-30 10X Genomics, Inc. Procédés et systèmes de traitement de cloisonnement
WO2016196691A2 (fr) 2015-06-01 2016-12-08 California Institute Of Technology Compositions et procédés pour l'analyse de lymphocytes t avec des antigènes pour des populations spécifiques
CN108289797B (zh) 2015-10-13 2022-01-28 哈佛学院院长及董事 用于制备和使用凝胶微球的系统和方法
EP3882357B1 (fr) 2015-12-04 2022-08-10 10X Genomics, Inc. Procédés et compositions pour l'analyse d'acide nucléique
US11081208B2 (en) 2016-02-11 2021-08-03 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017197343A2 (fr) 2016-05-12 2017-11-16 10X Genomics, Inc. Filtres microfluidiques sur puce
WO2017197338A1 (fr) 2016-05-13 2017-11-16 10X Genomics, Inc. Systèmes microfluidiques et procédés d'utilisation
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4310183B1 (fr) 2017-01-30 2025-07-09 10X Genomics, Inc. Procédés et systèmes de codage à barres de cellules uniques à base de gouttelettes
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
WO2018165475A1 (fr) 2017-03-08 2018-09-13 California Institute Of Technology Appariement de la spécificité antigénique d'une cellule t avec des séquences de récepteur de lymphocytes t
CN110870018B (zh) 2017-05-19 2024-11-22 10X基因组学有限公司 用于分析数据集的系统和方法
EP4230746A3 (fr) 2017-05-26 2023-11-01 10X Genomics, Inc. Analyse de cellule unique de chromatine accessible par transposase
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2019099751A1 (fr) 2017-11-15 2019-05-23 10X Genomics, Inc. Perles de gel fonctionnalisées
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
US12421558B2 (en) 2020-02-13 2025-09-23 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and DNA chromatin accessibility

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6057107A (en) * 1995-10-11 2000-05-02 Luminex Corporation Methods and compositions for flow cytometric determination of DNA sequences
WO2001014589A2 (fr) * 1999-08-20 2001-03-01 Luminex Corporation Technologie des reseaux liquides
US20020137060A1 (en) * 2000-08-09 2002-09-26 Brown-Augsburger Patricia Lea Combined hybridization-detection assays for determining nucleic acid concentrations in biological fluids
WO2003014382A2 (fr) * 2001-08-09 2003-02-20 Lambda Labor Für Molekularbiologische Dna-Analysen Gmbh Dispositif d'analyse d'acide nucleique
EP1548126A1 (fr) * 2003-12-22 2005-06-29 Bio-Rad Pasteur Support pour acide nucléique de contrôle et usage pour la détection des acides nucléiques.

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6057107A (en) * 1995-10-11 2000-05-02 Luminex Corporation Methods and compositions for flow cytometric determination of DNA sequences
WO2001014589A2 (fr) * 1999-08-20 2001-03-01 Luminex Corporation Technologie des reseaux liquides
US20020137060A1 (en) * 2000-08-09 2002-09-26 Brown-Augsburger Patricia Lea Combined hybridization-detection assays for determining nucleic acid concentrations in biological fluids
WO2003014382A2 (fr) * 2001-08-09 2003-02-20 Lambda Labor Für Molekularbiologische Dna-Analysen Gmbh Dispositif d'analyse d'acide nucleique
EP1548126A1 (fr) * 2003-12-22 2005-06-29 Bio-Rad Pasteur Support pour acide nucléique de contrôle et usage pour la détection des acides nucléiques.

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CAMILLA C ET AL: "Flow cytometric microsphere-based immunoassay: analysis of secreted cytokines in whole-blood samples from asthmatics.", CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY. JUL 2001, vol. 8, no. 4, July 2001 (2001-07-01), pages 776 - 784, XP002412740, ISSN: 1071-412X *
DUNBAR S A ET AL: "Quantitative, multiplexed detection of bacterial bacterial pathogens: DNA and protein applications of the Luminex LabMAP system", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM,, NL, vol. 53, May 2003 (2003-05-01), pages 245 - 252, XP002316963, ISSN: 0167-7012 *
FUJA TANNIN ET AL: "A multiplex microsphere bead assay for comparative RNA expression analysis using flow cytometry.", JOURNAL OF BIOTECHNOLOGY. 18 MAR 2004, vol. 108, no. 3, 18 March 2004 (2004-03-18), pages 193 - 205, XP002412741, ISSN: 0168-1656 *
ROCKENBAUER ESZTER ET AL: "SNP genotyping using microsphere-linked PNA and flow cytometric detection.", CYTOMETRY. PART A : THE JOURNAL OF THE INTERNATIONAL SOCIETY FOR ANALYTICAL CYTOLOGY. APR 2005, vol. 64, no. 2, April 2005 (2005-04-01), pages 80 - 86, XP002412743, ISSN: 1552-4922 *
SMITH P L ET AL: "A RAPID, SENSITIVE, MULTIPLEXED ASSAY FOR DETECTION OF VIRAL NUCLEIC ACIDS USING THE FLOW METRIX SYSTEM", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY, WASHINGTON, DC, US, vol. 44, no. 9, 1998, pages 2054 - 2056, XP002170109, ISSN: 0009-9147 *
WEDEMEYER N ET AL: "Flow cytometry: an 'old' tool for novel applications in medical genetics.", CLINICAL GENETICS. JUL 2001, vol. 60, no. 1, July 2001 (2001-07-01), pages 1 - 8, XP002412742, ISSN: 0009-9163 *

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