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WO2007010924A1 - Produit gélatinisé de cellules et système de préparation pour une biopuce de cellules ou de tissus à haute densité - Google Patents

Produit gélatinisé de cellules et système de préparation pour une biopuce de cellules ou de tissus à haute densité Download PDF

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Publication number
WO2007010924A1
WO2007010924A1 PCT/JP2006/314237 JP2006314237W WO2007010924A1 WO 2007010924 A1 WO2007010924 A1 WO 2007010924A1 JP 2006314237 W JP2006314237 W JP 2006314237W WO 2007010924 A1 WO2007010924 A1 WO 2007010924A1
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WO
WIPO (PCT)
Prior art keywords
cell
block
tissue
rows
container
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2006/314237
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English (en)
Japanese (ja)
Inventor
Jyunya Fukuoka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Toyama NUC
Original Assignee
University of Toyama NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Toyama NUC filed Critical University of Toyama NUC
Priority to JP2007526026A priority Critical patent/JPWO2007010924A1/ja
Publication of WO2007010924A1 publication Critical patent/WO2007010924A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples

Definitions

  • the present invention relates to a cell gel and a highly integrated cell for producing a cell array.
  • a tissue array is obtained by taking out a small section of tissue force embedded in paraffin and arranging a plurality of pieces on a glass slide.
  • Non-patent Document 1 devices using the method developed by Kononen et al. (Non-patent Document 1) are commercially available.
  • Non-Patent Document 2 Patent Document 1
  • Patent Document 2 Patent Document 2
  • the target tissue is taken out from the donor block of the paraffin-embedded tissue in a cylinder of several millimeters with a cylinder or the like.
  • the recipient block is made into thin slices and placed on a slide glass (Patent Document 1).
  • Patent Document 1 For cell arrays using cells instead of yarn and weave, for example, a method of producing force by suspending frozen cells by collecting cells collected from a surgical procedure or biopsy sample is known.
  • Non-patent document 2 Medical examination, 52 (6), 806-811 (2003)
  • Patent Document 1 Special Table 2004-500891
  • Patent Document 2 JP 2004-258017 A
  • frozen specimens have low storability of morphology and are suitable for observing minute structures under a microscope.
  • Tissue arrays and cell arrays are indispensable as research and treatment / diagnosis / prognostic determinants. Their production requires special machines and techniques.
  • the present inventor disperses surplus cells of a specimen submitted for daily cytodiagnosis and the like in a medium, and then adds mucopolysaccharide to the dispersion to obtain a gel gel. After that, a cell block was prepared using a formalin-treated cell gel, and a method for preparing a cell array from this cell block was devised.
  • Examples of the medium for dispersing cells include a liquid component of the specimen itself, a physiological saline, a phosphate buffered physiological saline, and the like.
  • mucopolysaccharide examples include hyaluronic acid, chondroitin, chondroitin sulfate, and alkali metal salts thereof.
  • Preferred is sodium hyaluronate.
  • the amount of mucopolysaccharide added is sufficient if the cell suspension is gelled, for example, 5 to 20 times the amount of cell suspension (WZV).
  • Formalin treatment may be performed, for example, using neutral buffered formalin 2-10 times the amount of gel (WZW) at room temperature for 6-12 hours.
  • the cell gel and the cell block of the present invention can be produced, for example, by the following method.
  • paraffin embedding is performed manually by a normal method.
  • n and m mean an integer of 3 to 10.
  • a hollow needle with an inner diameter of 1 to 5 mm for sample collection (a biopsy needle is acceptable) and a paraffin block (a) are distributed to many hospitals.
  • This container is divided to a size that fits a norafin block and consists of 5 rows x 5 rows or 10 rows x 10 rows!
  • each partition is 3.2 cm x 4.5 cm large, and the overall size of the box is approximately 16 cm x 22.5 cm x 3.5 cm or 32 cm x 45 cm x 3 It is 5cm.
  • tissue / cell embedded in the recovered paraffin block is transplanted to about 1000 tissue 'cell array blocks (second block) by an arrayer, for example, Beecher TM Arrayer.
  • an arrayer for example, Beecher TM Arrayer.
  • a good quality tissue of about 900 to 1000 sheets.
  • Cell array slides can be sliced.
  • a cell paraffin block can be created even if sufficient cells are not obtained from the surplus specimens of specimens submitted for daily cytodiagnosis.
  • it is possible to easily construct a system for producing an array by storing tissue 'collecting a sample for a cell array' at a large number of facilities and collecting them in one place. I'll do it.
  • FIG. 1 is a photograph of a donor block.
  • FIG.2 Photograph of stained cell / tissue array.
  • the cell block is prepared by the method of Example 1 or the like, or Remove the necessary part from the tissue block itself with a hollow needle with a diameter of 2 mm or a biopsy needle, and put the contents into the hole of the donor block.
  • the sample is removed from the donor block by using an array production device, for example, Beecher TM Arrayer (manufactured by Beecher Instruments). Transplanted to the recipient block).
  • an array production device for example, Beecher TM Arrayer (manufactured by Beecher Instruments).
  • the tape is not removed until dyeing or the like.
  • Protein profiles for each disease can be created, and these clusters will elucidate the factors that determine treatment, diagnosis, and prognosis, and contribute to new treatment selection 'disease classification'.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention a pour objet un procédé pour la préparation d'un bloc de cellules à partir de cellules dispersées dans un milieu tel qu'un composant liquide d'un échantillon, destiné à être utilisé lors de la préparation d'une biopuce de cellules ; et un système pour la préparation d'une biopuce de cellules/tissus à haute densité. On disperse dans un milieu une portion non utilisée des cellules présentes dans un échantillon soumis à un diagnostic de routine basé sur les cellules ou similaire, on ajoute un mucopolysaccharide au milieu pour provoquer la gélification du milieu, on traite avec de la formaline la matière à base de cellules gélifiée et ensuite on utilise la matière à base de cellules gélifiée et traitée avec de la formaline pour préparer un bloc de cellules. En utilisant un bloc de cellules et/ou un bloc de tissus préparé de cette manière, il devient possible de recueillir/stocker des échantillons de biopuce de tissus/cellules dans un grand nombre d'installations, de rassembler les échantillons dans un seul endroit et de construire un système pour la préparation de biopuces de tissus/cellules à haute densité.
PCT/JP2006/314237 2005-07-21 2006-07-19 Produit gélatinisé de cellules et système de préparation pour une biopuce de cellules ou de tissus à haute densité Ceased WO2007010924A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2007526026A JPWO2007010924A1 (ja) 2005-07-21 2006-07-19 細胞ゲル化物及び高集積の、細胞又は組織アレイの作製システム

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2005210737 2005-07-21
JP2005-210737 2005-07-21

Publications (1)

Publication Number Publication Date
WO2007010924A1 true WO2007010924A1 (fr) 2007-01-25

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2006/314237 Ceased WO2007010924A1 (fr) 2005-07-21 2006-07-19 Produit gélatinisé de cellules et système de préparation pour une biopuce de cellules ou de tissus à haute densité

Country Status (2)

Country Link
JP (1) JPWO2007010924A1 (fr)
WO (1) WO2007010924A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008123410A1 (fr) * 2007-03-29 2008-10-16 National University Corporation University Of Toyama Dispositif pour stocker une tranche d'échantillon et instrument pour une observation microscopique équipé de celui-ci
JP2009002768A (ja) * 2007-06-21 2009-01-08 Japan Health Science Foundation 組織マイクロアレイ作製方法
JP2011013050A (ja) * 2009-06-30 2011-01-20 Pathology Institute 生体試料標本の作製方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10501706A (ja) * 1994-04-04 1998-02-17 コラーゲン コーポレイション 細胞−ゲル
JP2002522570A (ja) * 1998-08-05 2002-07-23 ジャスパー、リミテッド、ライアビリティ、カンパニー ヒアルロン酸と天然型アミノ酸との反応生成物、ならびに化粧品および医薬組成物への使用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001270111A1 (en) * 2000-06-22 2002-01-02 Clinomics Laboratories, Inc. Frozen tissue microarrays and methods for using the same
JP3877213B2 (ja) * 2003-02-07 2007-02-07 康彦 北山 アレイブロック作成方法とこれに使用される組織くりぬき装置

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10501706A (ja) * 1994-04-04 1998-02-17 コラーゲン コーポレイション 細胞−ゲル
JP2002522570A (ja) * 1998-08-05 2002-07-23 ジャスパー、リミテッド、ライアビリティ、カンパニー ヒアルロン酸と天然型アミノ酸との反応生成物、ならびに化粧品および医薬組成物への使用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KOH W.-G. ET AL.: "Molding of hydrogel microstructures to create multiphenotype cell microarrays", ANAL. CHEM., vol. 75, no. 21, 2003, pages 5783 - 5789, XP001047336 *
KONONEN J. ET AL.: "Tissue microarrays for high-throughput molecular profiling of tumor specimens", NATURE MEDICINE, vol. 4, no. 7, July 1998 (1998-07-01), pages 844 - 847, XP002927997 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008123410A1 (fr) * 2007-03-29 2008-10-16 National University Corporation University Of Toyama Dispositif pour stocker une tranche d'échantillon et instrument pour une observation microscopique équipé de celui-ci
JP5008208B2 (ja) * 2007-03-29 2012-08-22 国立大学法人富山大学 検体薄片の保存具及びこれを備えた顕微鏡観察用具
JP2009002768A (ja) * 2007-06-21 2009-01-08 Japan Health Science Foundation 組織マイクロアレイ作製方法
JP2011013050A (ja) * 2009-06-30 2011-01-20 Pathology Institute 生体試料標本の作製方法

Also Published As

Publication number Publication date
JPWO2007010924A1 (ja) 2009-01-29

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