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WO2007010532A3 - Compositions for one step colorimetric analysis and methods of using same - Google Patents

Compositions for one step colorimetric analysis and methods of using same Download PDF

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Publication number
WO2007010532A3
WO2007010532A3 PCT/IL2006/000830 IL2006000830W WO2007010532A3 WO 2007010532 A3 WO2007010532 A3 WO 2007010532A3 IL 2006000830 W IL2006000830 W IL 2006000830W WO 2007010532 A3 WO2007010532 A3 WO 2007010532A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
methods
same
colorimetric analysis
kits
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IL2006/000830
Other languages
French (fr)
Other versions
WO2007010532A2 (en
Inventor
Shmuel Bukshpan
Gleb Zilberstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2007010532A2 publication Critical patent/WO2007010532A2/en
Publication of WO2007010532A3 publication Critical patent/WO2007010532A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides compositions and kits comprising porous substrates that retain staining solutions, wherein the staining solutions comprise one or more staining agents that form visible complexes with proteins or other molecular species of interest in a gel. The invention further provides methods for identifying biological moieties in a sample, using the compositions and kits of the invention, with superior signal to noise ratios that do not require destaining procedures.
PCT/IL2006/000830 2005-07-18 2006-07-18 Compositions for one step colorimetric analysis and methods of using same Ceased WO2007010532A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70002705P 2005-07-18 2005-07-18
US60/700,027 2005-07-18

Publications (2)

Publication Number Publication Date
WO2007010532A2 WO2007010532A2 (en) 2007-01-25
WO2007010532A3 true WO2007010532A3 (en) 2008-01-10

Family

ID=37669230

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2006/000830 Ceased WO2007010532A2 (en) 2005-07-18 2006-07-18 Compositions for one step colorimetric analysis and methods of using same

Country Status (1)

Country Link
WO (1) WO2007010532A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776684A (en) * 1995-12-28 1998-07-07 Edvotek Method for staining biomolecules using a gelled matrix
WO1999042620A2 (en) * 1998-02-19 1999-08-26 Edvotek Articles of manufacture and methods for staining biomolecules
US20010044108A1 (en) * 2000-01-28 2001-11-22 Lih-Bin Shih Detection of biomolecules in gels following electrophoresis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776684A (en) * 1995-12-28 1998-07-07 Edvotek Method for staining biomolecules using a gelled matrix
WO1999042620A2 (en) * 1998-02-19 1999-08-26 Edvotek Articles of manufacture and methods for staining biomolecules
US20010044108A1 (en) * 2000-01-28 2001-11-22 Lih-Bin Shih Detection of biomolecules in gels following electrophoresis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PATTON W.F.: "Detection technologies in proteome analysis", J. CHROMATOGRAPHY B, vol. 771, 2002, pages 3 - 31, XP004350527, DOI: doi:10.1016/S1570-0232(02)00043-0 *

Also Published As

Publication number Publication date
WO2007010532A2 (en) 2007-01-25

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