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WO2007099684A1 - Melangeur et analyseur - Google Patents

Melangeur et analyseur Download PDF

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Publication number
WO2007099684A1
WO2007099684A1 PCT/JP2006/325637 JP2006325637W WO2007099684A1 WO 2007099684 A1 WO2007099684 A1 WO 2007099684A1 JP 2006325637 W JP2006325637 W JP 2006325637W WO 2007099684 A1 WO2007099684 A1 WO 2007099684A1
Authority
WO
WIPO (PCT)
Prior art keywords
liquid
acoustic wave
surface acoustic
sound
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2006/325637
Other languages
English (en)
Japanese (ja)
Inventor
Miyuki Murakami
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp filed Critical Olympus Corp
Publication of WO2007099684A1 publication Critical patent/WO2007099684A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/80—Mixing by means of high-frequency vibrations above one kHz, e.g. ultrasonic vibrations
    • B01F31/86—Mixing by means of high-frequency vibrations above one kHz, e.g. ultrasonic vibrations with vibration of the receptacle or part of it
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • G01N2035/00554—Mixing by a special element, e.g. stirrer using ultrasound

Definitions

  • the present invention relates to a stirrer and an analyzer.
  • a reaction vessel is placed in a bath holding constant temperature water, and the liquid held in the reaction vessel is stirred and mixed by sound waves emitted from a sound source placed in a bath outside the reaction vessel. It is carried out.
  • Patent Document 1 Japanese Patent No. 3168886
  • the analyzer disclosed in Patent Document 1 has a problem that the sound source and the reaction container are spaced apart via constant temperature water, and the liquid stirring efficiency is poor due to attenuation due to absorption of sound waves by the constant temperature water. was there.
  • the analyzer of Patent Document 1 since the sound source of the stirrer that is liquid-tightly attached to the bathtub is driven by a wired driver, the analyzer of Patent Document 1 is complicated in structure and difficult to downsize. There was a problem.
  • the present invention has been made in view of the above, and provides a stirring device and an analysis device that can improve the stirring efficiency of a liquid S, have a simple structure, and can be downsized. Purpose.
  • one aspect of the stirring device of the present invention includes a container that holds a liquid to be stirred, and irradiates the liquid with sound waves. And a sound wave generating means for stirring the liquid, wherein the sound wave generating means is formed on the piezoelectric substrate and has a contact surface in contact with the container, and stirs the liquid.
  • a sound generating unit that generates sound waves, and the contact on the piezoelectric substrate And a power receiving unit which is formed in a portion other than the surface and wirelessly receives the driving power of the sound generating unit supplied from the outside.
  • the sound generating portion is formed together with the power receiving portion on a surface on the piezoelectric substrate facing the contact surface. To do.
  • the power receiving unit is formed on an outer periphery of the piezoelectric substrate.
  • the sound generation unit is formed on the contact surface
  • the power reception unit is formed on a surface on the piezoelectric substrate facing the contact surface. It is characterized by being formed.
  • the power reception unit is a reception antenna.
  • the sound generation unit is a comb-like electrode.
  • the sound wave is a surface elastic wave or a Balta wave.
  • One aspect of the stirring device of the present invention is characterized in that, in the above invention, the sound wave generating means is fixed to the container.
  • the sound wave generating means contacts the outside of the container when stirring the liquid.
  • one embodiment of the analyzer of the present invention measures a plurality of different liquids while stirring and reacting them, and measures the optical characteristics of the reaction liquid.
  • the analysis device for analyzing the reaction solution is characterized in that the reaction solution of the sample and the reagent is optically analyzed using the stirring device.
  • the sound wave generating means including the piezoelectric substrate in which the sound generating portion is formed on the piezoelectric substrate and the power receiving portion is formed in a portion other than the contact surface on the piezoelectric substrate is formed by the contact surface.
  • the analyzer is attached to the container, and the sound generation unit that is the sound source is close to the container.
  • the stirring device and the analysis device of the present invention have a short acoustic wave propagation path, which suppresses the attenuation of the sound wave.
  • the liquid stirring efficiency can be improved, the structure is simple, and the size can be reduced.
  • the stirring device and the analysis device of the present invention are configured to receive power on the surface other than the surface in contact with the container, it is possible to facilitate power supply from the outside and secure high wireless transmission efficiency. Have a positive effect.
  • FIG. 1 is a schematic configuration diagram showing an automatic analyzer according to a first embodiment.
  • FIG. 2 is a perspective view showing a part of a reaction vessel and a reaction wheel used in the automatic analyzer according to the first embodiment together with a schematic configuration diagram of a stirrer.
  • FIG. 3 is a diagram showing a block diagram showing a configuration of the stirrer of Embodiment 1 together with a perspective view of a reaction vessel.
  • FIG. 4 is a perspective view of a surface acoustic wave device attached to the side wall of the reaction vessel of FIG.
  • FIG. 5 is a cross-sectional view of the reaction vessel shown in FIG. 3, showing the flow that occurs in the retained liquid.
  • FIG. 6 is an enlarged view of part A of the reaction vessel shown in FIG.
  • FIG. 7 is a perspective view showing a first modification of the surface acoustic wave device used in the stirring device of the present invention.
  • FIG. 8 is a perspective view showing a second modification of the surface acoustic wave device used in the stirring device of the present invention.
  • FIG. 9 is a cross-sectional view of the reaction vessel showing the flow generated in the liquid held by the reaction vessel in the stirring device using the surface acoustic wave device of FIG.
  • FIG. 10 is an enlarged view of the vicinity of the surface acoustic wave device of the reaction vessel shown in FIG.
  • FIG. 11 is a cross-sectional view showing a first modification of the reaction vessel used in the stirring apparatus of the present invention.
  • FIG. 12 is a cross-sectional view showing a second modification of the reaction vessel used in the stirring apparatus of the present invention.
  • FIG. 13 is a cross-sectional view showing a third modification of the reaction vessel used in the stirring apparatus of the present invention.
  • FIG. 14 is a cross-sectional view showing a fourth modification of the reaction vessel used in the stirring apparatus of the present invention.
  • FIG. 15 is a front view of the surface acoustic wave device used in the reaction vessel shown in FIG. 11.
  • FIG. 16 is an enlarged view of part B of the reaction vessel shown in FIG. 11.
  • FIG. 17 is a cross-sectional view showing a fifth modification of the reaction vessel used in the stirring apparatus of the present invention.
  • FIG. 18 is a schematic configuration diagram of the automatic analyzer according to the second embodiment provided with a stirring device.
  • FIG. 19 is a block diagram showing the configuration of the automatic analyzer of FIG. 18 and the stirring device of the second embodiment.
  • FIG. 20 is a perspective view showing a reaction vessel used in the automatic analyzer shown in FIG. 15 together with a power transmission body provided with an arm member.
  • FIG. 21 is a view showing a state in which the acoustic matching liquid is discharged to the surface acoustic wave device, with a cross section of the arm member, a part of the reaction wheel, and the reaction vessel.
  • FIG. 22 is a view showing a state where the drive arm is extended and the surface acoustic wave element at the end face is brought into contact with the side wall of the reaction vessel in FIG.
  • FIG. 1 is a schematic configuration diagram showing the automatic analyzer according to the first embodiment.
  • FIG. 2 is a perspective view showing a part of a reaction vessel and a reaction wheel used in the automatic analyzer according to the first embodiment together with a schematic configuration diagram of a stirring device.
  • FIG. 3 is a view showing a block diagram showing a configuration of the stirring device of the first embodiment together with a perspective view of the reaction vessel.
  • FIG. 4 is a perspective view of a surface acoustic wave device attached to the side wall of the reaction vessel of FIG.
  • the automatic analyzer 1 includes a sample table 3, a sample dispensing mechanism 5, a reaction wheel 6, a photometric device 10, a washing device 11, a reagent dispensing mechanism 12, and a test table 2 on a work table 2.
  • a medicine table 13 is provided and a stirring device 20 is provided.
  • the sample table 3 is rotated in the direction indicated by the arrow by the driving means, and a plurality of storage chambers 3a are provided on the outer periphery at equal intervals along the circumferential direction. .
  • a sample container 4 containing a sample is detachably stored in each storage chamber 3a.
  • the sample dispensing mechanism 5 is a means for dispensing a sample into a plurality of reaction containers 7 held by the reaction wheel 6. As shown in FIG. Dispense sequentially into the reaction vessel 7.
  • the reaction wheel 6 is rotated in a direction indicated by an arrow in FIG. 1 by a driving means different from the sample table 3, and a plurality of recesses 6a are provided at equal intervals along the circumferential direction on the outer periphery.
  • the reaction wheel 6 has openings 6b (see FIG. 2) through which measurement light passes on both radial sides of the recesses 6a.
  • the reaction wheel 6 rotates clockwise by one cycle (one reaction container per round) and rotates counterclockwise by one recess 6a in four cycles.
  • a photometric device 10 and a cleaning device 11 are arranged on the rotation path, and a stirring device is arranged at the bottom. 20 are arranged.
  • the reaction container 7 is a very small container with a capacity of several nL to several tens of ⁇ L, and 80% or more of the light contained in the analysis light (340 to 800 nm) emitted from the light source of the photometric device 10 is obtained.
  • Transparent materials such as glass containing heat-resistant glass, synthetic resin such as cyclic olefin and polystyrene are used.
  • the reaction vessel 7 has a liquid holding portion 7d (see FIG. 5) having a square horizontal cross section for holding the liquid by the side walls 7a, 7b and the bottom wall 7c (see FIG. 5). This is a square tube-shaped cuvette formed and having an opening 7e above the liquid holding portion 7d.
  • the reaction container 7 constitutes a stirrer 20 together with the surface acoustic wave element 22 attached to the side wall 7a, and the inner surface of the liquid holding part 7d is subjected to affinity processing for a liquid such as a specimen or a reagent.
  • the reaction vessel 7 is disposed in the recess 6 a with the side wall 7 a facing the radial direction of the reaction wheel 6 and the side wall 7 b facing the circumferential direction of the reaction wheel 6.
  • the photometric device 10 is disposed near the outer periphery of the reaction wheel 6 and emits analysis light (340 to 800 nm) for analyzing the liquid held in the reaction vessel 7; And a light receiver for spectroscopically receiving the analysis light transmitted through the liquid.
  • the light source and the light receiver are arranged at positions where they oppose each other in the radial direction across the recess 6 a of the reaction wheel 6.
  • the cleaning device 11 has a discharging means for discharging the liquid and the cleaning liquid from the reaction vessel 7, and a cleaning liquid dispensing means.
  • the cleaning device 11 discharges the liquid after the photometry from the reaction container 7 after the photometry is completed, and then dispenses the cleaning liquid.
  • the cleaning device 11 cleans the inside of the reaction vessel 7 by repeating the dispensing and discharging operations of the cleaning solution several times. The reaction container 7 washed in this way is used again for the analysis of a new specimen.
  • the reagent dispensing mechanism 12 is means for dispensing a reagent to a plurality of reaction containers 7 held by the reaction wheel 6. As shown in FIG. Are sequentially dispensed into the reaction vessel 7.
  • the reagent table 13 is rotated in a direction indicated by an arrow in FIG. 1 by a driving means different from the sample table 3 and the reaction wheel 6, and a plurality of storage chambers 13a formed in a fan shape are provided along the circumferential direction. It has been.
  • the reagent container 14 is detachably stored in each storage chamber 13a.
  • Each of the plurality of reagent containers 14 is filled with a predetermined reagent corresponding to the inspection item, and is formed on the outer surface.
  • An information recording medium (not shown) for displaying information on the contained reagent is affixed!
  • the control unit 16 includes a sample table 3, a sample dispensing mechanism 5, a reaction wheel 6, a photometric device 10, a washing device 11, a reagent dispensing mechanism 12, a reagent table 13, a reading device 15, an analysis unit 17, and an input.
  • a microcomputer or the like that is connected to the unit 18, the display unit 19, the stirring device 20, and the like and has a storage function for storing analysis results is used.
  • the control unit 16 controls the operation of each part of the automatic analyzer 1, and based on the information read from the record on the information recording medium, if the reagent lot or expiration date is outside the installation range, the analysis work is performed.
  • the automatic analyzer 1 is controlled to stop the operation or a warning is given to the operator.
  • the analysis unit 17 is connected to the photometry device 10 via the control unit 16, and analyzes the component concentration of the specimen from the absorbance of the liquid in the reaction container 7 based on the amount of light received by the light receiver, and the analysis result is obtained. Output to control unit 16.
  • the input unit 18 is a part that performs an operation of inputting inspection items and the like to the control unit 16, and for example, a keyboard and a mouse are used.
  • the display unit 19 displays analysis contents and alarms, and a display panel or the like is used.
  • the stirrer 20 stirs the liquid held in the reaction vessel 7 by sound waves generated by driving the surface acoustic wave element 22, and in addition to the reaction vessel 7, as shown in FIG. 2 and FIG.
  • a power transmitting body 21 that transmits power to the surface acoustic wave element 22 and a surface acoustic wave element 22 are provided.
  • the power transmission body 21 includes an RF transmission antenna 21a, a drive circuit 21b, and a controller 21c.
  • the power transmitting body 21 transmits power supplied from the RF transmitting antenna 21a to the surface acoustic wave element 22 as a driving signal from a high frequency AC power supply of several MHz to several hundred MHz.
  • the RF transmitting antenna 21a is attached to the side wall of the recess 6a of the reaction wheel 6.
  • the drive circuit 21b has an oscillation circuit whose oscillation frequency can be changed based on a control signal from the controller 21c.
  • a high-frequency oscillation signal of about several tens to several hundreds of MHz is supplied to the RF transmission antenna 21a.
  • the RF transmitting antenna 21a and the driving circuit 21b Are connected via contact electrodes so that electric power is transmitted even when the reaction wheel 6 rotates.
  • the RF transmission antenna 21a to which power is transmitted via the contact electrode is switched as the reaction wheel 6 rotates, and the liquid held in the reaction container 6 in each recess 6a is sequentially stirred.
  • the controller 21c controls the operation of the drive circuit 21b.
  • the characteristics (frequency, intensity, phase, wave characteristics) and waveform (sine wave, triangle wave, rectangular wave, burst wave) generated by the surface acoustic wave element 22 are controlled. Etc.) or modulation (amplitude modulation, frequency modulation), etc.
  • the controller 21c can switch the frequency of the oscillation signal generated by the drive circuit 2 lb according to the built-in timer.
  • the surface acoustic wave element 22 is a sound wave generator that receives a drive signal (electric power) transmitted from the RF transmission antenna 21a and generates a sound wave.
  • the surface acoustic wave element 22 is composed of a vibrator 22b and an antenna formed on a same surface of a piezoelectric substrate 22a having a uniform force of lithium niobate (LiNb03) and a comb-like electrode (IDT). 22c is formed.
  • the resonator 22b and the antenna 22c are formed on a surface facing the contact surface F (see FIG. 6) of the piezoelectric substrate 22a for attaching the surface acoustic wave element 22 to the reaction vessel 7.
  • the transducer 22b is a sounding unit that generates sound waves by receiving a drive signal (power) transmitted from the RF transmission antenna 21a by the antenna 22c.
  • the vibrator 22b is disposed outside the reaction vessel 7 adjacent to the liquid held by the reaction vessel 7 via the reaction vessel 7 and the piezoelectric substrate 22a. That is, as shown in FIGS. 5 and 6, the surface elastic wave element 22 has a transducer 22b and an antenna 22c facing outward, and a reaction container through an acoustic matching layer 23 such as epoxy resin or UV-cured resin. 7 is attached to the side wall 7a.
  • the surface acoustic wave element 22 is schematically shown by ignoring the actual thickness in order to clarify the configuration including the thickness of the acoustic matching layer 23 in addition to the thickness of the piezoelectric substrate 22a, the vibrator 22b, and the antenna 22c. The same applies to the other embodiments.
  • the reagent dispensing mechanism 12 supplies the reagent from the reagent container 14 to the plurality of reaction containers 7 conveyed along the circumferential direction by the rotating reaction wheel 6. Dispense sequentially.
  • the reaction container 7 into which the reagent has been dispensed is transported along the circumferential direction by the reaction wheel 6, and the specimen is sequentially dispensed from the plurality of specimen containers 4 held in the specimen table 3 by the specimen dispensing mechanism 5. .
  • the reaction container 7 into which the sample has been dispensed is transported to the stirring device 20 by the reaction wheel 6, and the dispensed reagent and the sample are sequentially stirred. React.
  • the reaction solution in which the sample and the reagent have reacted in this way passes through the photometric device 10 when the reaction wheel 6 rotates again, and the analysis light emitted from the light source is transmitted. At this time, the reaction solution of the reagent and the sample in the reaction container 7 is measured by the light receiving unit, and the component concentration and the like are analyzed by the control unit 16. Then, after the analysis is completed, the reaction vessel 7 is washed by the washing device 11 and then used again for analyzing the specimen.
  • the controller 21c inputs a drive signal to the drive circuit 21b when the reaction wheel 6 is stopped based on the control signal input in advance from the input unit 18 via the control unit 16.
  • the vibrator 22b is driven in accordance with the frequency of the input drive signal, and a Balta wave Wb is induced as shown in FIG.
  • the induced Balta wave Wb propagates through the piezoelectric substrate 22a and the acoustic matching layer 23, then enters the side wall 7a of the reaction vessel 7, propagates through the side wall 7a as indicated by the arrow, and then has a liquid having a close acoustic impedance. Leaking to L.
  • the vibrator 22b is directed to the side wall 7a adjacent to the liquid L, and the surface acoustic wave element 22 is attached to the side wall 7a via the acoustic matching layer 23. Therefore, in the stirring device 20 and the automatic analyzer 1, the vibrator 22b and the reaction vessel 7 are in contact with each other, and the sound wave generated by the vibrator 22b is incident on the adjacent liquid L through the side wall 7a. Therefore, since the stirrer 20 and the automatic analyzer 1 can suppress the attenuation of the sound wave on the propagation path where the sound wave propagation path is short, the stirring efficiency of the liquid can be improved. Further, the stirring device 20 and the automatic analyzer 1 can efficiently supply power because the antenna 22c of the surface acoustic wave element 22 is disposed outside the piezoelectric substrate 22a.
  • the surface acoustic wave element 22 uses a comb-like electrode (IDT) as the vibrator 22b and is supplied with power by radio, so the structure is simple and small. With a simple configuration, the size can be reduced, and the stirring device 20, and hence the automatic analyzer 1, can be reduced in size. Further, since the surface acoustic wave element 22 is provided with the vibrator 22b and the antenna 22c on the same surface of the piezoelectric substrate 22a, the vibrator 22b and the antenna 22c can be formed at a time. It has the advantage that it can be manufactured easily. However, since the stirring device 20 supplies power to the surface acoustic wave element 22 wirelessly, there are fewer failures compared to the case of the wired power supply that supplies power when the components are in electrical contact. .
  • IDT comb-like electrode
  • the stirring device 20 may be provided with an antenna 24c on the outer periphery other than the contact surface of the piezoelectric substrate 24a.
  • the surface acoustic wave element 24 connects the transducer 24b and the antenna 24c with the bus bar 24d.
  • the antenna 24c is provided on the outer periphery of the piezoelectric substrate 24a, the surface acoustic wave element 24 vibrates the piezoelectric substrate 24a, and hence the surface acoustic wave element 24 itself, as is apparent from the comparison with the surface acoustic wave element 22 shown in FIG.
  • the size can be reduced according to the size of the child 22b, and the degree of freedom in arrangement increases, so the degree of freedom in design of the stirring device 20 increases.
  • the agitation device 20 may use a surface acoustic wave element 25 shown in FIG.
  • the surface acoustic wave element 25 includes an oscillator 25b and a bus bar 25c formed on one contact surface F of a piezoelectric substrate 25a to be attached in contact with the reaction vessel 7, and the bus bar 25c extending to the other surface. 25d is formed.
  • the surface acoustic wave element 25 is arranged so that when the reaction container 7 is set in the automatic analyzer 1, the surface acoustic wave element 25 is arranged so that the plurality of comb-like electrodes constituting the vibrator 25b are arranged in the vertical direction. Attach to 7 side wall 7a.
  • the surface acoustic wave element 25 has the acoustic matching layer 23 such as an epoxy resin, an ultraviolet-cured resin, and the like, with the transducer 25b facing the side wall 7a and the antenna 25d facing outward (see FIGS. 9 and 10). Is attached to the side wall 7a of the reaction vessel 7.
  • the acoustic matching layer 23 such as an epoxy resin, an ultraviolet-cured resin, and the like
  • the surface acoustic wave induced by driving the surface acoustic wave element 25 propagates from the acoustic matching layer 23 into the side wall 7a of the reaction vessel 7, and the acoustic impedance is increased. Leaks into a nearby liquid.
  • the flow fee is directed obliquely upward and directed obliquely downward. Flow Few force is generated respectively. With these two flows, the liquid L held in the reaction vessel 7 is agitated.
  • the vibrator 25b is directed to the side wall 7a adjacent to the liquid L, and the surface acoustic wave element 25 is attached to the side wall 7a via the acoustic matching layer 23 (see Figs. 9 and 10).
  • the stirring device 20 uses the acoustic matching layer 23 for the surface acoustic wave generated by the vibrator 25b. Enters the adjacent liquid L through the side wall 7a.
  • the agitator 20 is in contact with the vibrator 25b and the side wall 7a and the propagation path of the surface acoustic wave is short, the attenuation of the surface acoustic wave is suppressed, so that the use efficiency of the surface acoustic wave and the stirring efficiency of the liquid L are reduced. Can be improved.
  • the stirring device 20 of the present invention uses a comb-like electrode (IDT) as the vibrator 22b, the structure of the surface acoustic wave element 22 is simple, and the surface acoustic wave element 22 and the power transmission The body 21 can be miniaturized. Further, since the surface acoustic wave device 22 is fixed to the reaction vessel 7 in the stirring device 20, the surface acoustic wave device 22 can be easily handled together with the reaction vessel 7.
  • IDT comb-like electrode
  • the stirrer 20 has a concave portion 7f formed with a thin side wall 7a, and the vibrator 22b is directed to the outside of the reaction vessel 7, and the surface elasticity is passed through the acoustic matching layer.
  • the reaction vessel 7 in which the wave element 22 is embedded in the recess 7f may be used, and the liquid may be agitated by Balta waves.
  • the stirring device 20 may be provided with two vibrators 22b on the upper and lower sides of the surface acoustic wave element 22 as in the reaction vessel 7 shown in FIG.
  • the stirring device 20 drives the two vibrators 22b in a time-sharing manner according to the amount of liquid held in the reaction vessel 7, or simultaneously drives the vibrators 22b with different center frequencies.
  • the stirring ability can be improved, and the liquid can be stirred in a short time even when a large amount of liquid is held.
  • the stirrer 20 is like a reaction vessel 7 shown in FIG.
  • the surface acoustic wave element 22 may be used as a part of the side wall 7a, and the surface acoustic wave element 22 may be embedded in the upper part of the side wall 7a with the vibrator 22b facing the outside of the reaction vessel 7.
  • the stirring device 20 may have the surface acoustic wave element 26 attached to the lower surface of the bottom wall 7c as in the reaction vessel 7 shown in FIG. As shown in FIG.
  • a vibrator 26b having an interdigital electrode (IDT) force is provided at the center of the surface of 6a, and an antenna 26c serving as a power receiving means is provided integrally so as to surround the vibrator 26b.
  • the surface acoustic wave element 26 uses a norm wave by attaching the vibrator 26b to the outside of the reaction vessel 7 and attaching it to the bottom wall 7c via the acoustic matching layer 23. Stir the liquid.
  • the agitator 20 also connects the RF transmission antenna 21a of the power transmission body 21 to the reaction wheel 6. Recess 6a is provided on the bottom wall.
  • the reaction vessel 7 can be reduced in size by using the piezoelectric substrate 25a of the surface acoustic wave element 25 as the bottom wall as in the reaction vessel 7 shown in FIG.
  • the surface acoustic wave element 26 attaches the piezoelectric substrate 26a to the lower part of the side wall 7a with the vibrator 26b facing the outside of the reaction vessel 7.
  • the stirring device 20 may use the surface acoustic wave element 24 provided with the antenna 24c on the outer periphery of the piezoelectric substrate 24a shown in FIG. Oh ,.
  • FIG. 18 is a schematic configuration diagram of the automatic analyzer according to the second embodiment provided with a stirring device.
  • FIG. 19 is a block diagram showing configurations of the automatic analyzer of FIG. 18 and the stirring device of the second embodiment.
  • FIG. 20 is a perspective view showing a reaction container used in the automatic analyzer of FIG. 15 together with a power transmission body provided with an arm member.
  • the automatic analyzer of the second embodiment uses the same reaction vessel and surface acoustic wave element as the stirring device 20 of the first embodiment, the reaction vessel and the surface acoustic wave element are used. It is described using the same symbol
  • the automatic analyzer 30 includes a reagent table 31, 32, a reaction wheel 33, a specimen container transfer mechanism 37, a photometric system 42, a cleaning mechanism 43, a control unit 45, and a stirrer. 50 is provided.
  • the reagent tables 31 and 32 hold a plurality of reagent containers 31a and 32a arranged in the circumferential direction, respectively, and are rotated by driving means (not shown) to surround the reagent containers 31a and 32a. Transport in the direction.
  • the reaction wheel 33 has a plurality of reaction vessels 7 arranged in the circumferential direction and is rotated forward or reverse by a drive means (not shown). Transport.
  • the reagent is dispensed from the reagent containers 31a and 32a of the reagent tables 31 and 32 by the reagent dispensing mechanisms 35 and 36 provided in the vicinity.
  • the reagent dispensing mechanisms 35 and 36 are respectively Probes 35b and 36b for dispensing the reagent are provided on the arms 35a and 36a that rotate in the direction of the arrow in the horizontal plane, and have cleaning means for cleaning the probes 35b and 36b with cleaning water.
  • the reaction vessel 7 constitutes a stirring device 50 together with the surface acoustic wave element 25 attached to the side wall 7a.
  • the specimen container transfer mechanism 37 is a transfer means for transferring a plurality of racks 39 arranged in the feeder 38 one by one along the direction of the arrow. Transport.
  • the rack 39 holds a plurality of sample containers 39a containing samples.
  • the sample container 39a is moved by the sample dispensing mechanism 41 having a drive arm 41a and a probe 41b that rotate horizontally. Is dispensed into each reaction vessel 7. For this reason, the specimen dispensing mechanism 41 has a cleaning means for cleaning the probe 41b with cleaning water.
  • the photometric system 42 emits analysis light (340 to 800 nm) for analyzing the liquid in the reaction vessel 7 where the reagent and the sample have reacted. As shown in FIG. It has a spectroscopic part 42b and a light receiving part 42c. The analysis light emitted from the light emitting part 42a passes through the liquid in the reaction vessel 7, and is received by the light receiving part 42c provided at a position facing the spectroscopic part 42b. The light receiving unit 42c is connected to the control unit 45.
  • the cleaning mechanism 43 sucks and discharges the liquid in the reaction vessel 7 with the nozzle 43a, and then repeatedly injects and sucks cleaning liquid such as detergent and cleaning water with the nozzle 43a, thereby sucking the photometry system 42. Wash the reaction vessel 7 for which the analysis is completed.
  • the control unit 45 controls the operation of each part of the automatic analyzer 30, and based on the absorbance of the liquid in the reaction container 7 based on the amount of light emitted from the light emitting unit 42a and the amount of light received by the light receiving unit 42c. Analyzing the component concentration of the specimen, for example, a microcomputer or the like is used. As shown in FIGS. 18 and 19, the control unit 45 is connected to an input unit 46 such as a keyboard and a display unit 47 such as a display panel.
  • the stirring device 50 stirs the liquid held in the reaction vessel 7 by sound waves generated by driving the surface acoustic wave element 25.
  • a power transmission body 51 and a surface acoustic wave element 25 are provided.
  • the power transmitter 51 has a reaction wheel 33 A high frequency alternating current power supply of about several MHz to several hundred MHz is arranged at the outer periphery facing the reaction vessel 7 in the horizontal direction, and the supplied electric power is transmitted to the surface acoustic wave device 25.
  • the power transmission body 51 includes a drive circuit and a controller, and an arm member 57 is provided as shown in FIG.
  • the arm member 57 has a surface acoustic wave element 25 attached to its tip. At this time, the power transmission body 51 is supported by the arrangement determining member 52 as shown in FIG. 18, and transmits power to the surface acoustic wave element 25 when the reaction wheel 33 stops rotating.
  • the operation of the arrangement determining member 52 is controlled by the control unit 45, and when transmitting power from the power transmitting body 51 to the surface acoustic wave element 25, the power transmitting body 51 is moved to move the power transmitting body 51 and the reaction container 7
  • a two-axis stage is used to adjust the relative arrangement of the reaction wheel 33 in the circumferential direction and the radial direction.
  • the arrangement determining member 52 is stopped when the reaction wheel 33 rotates and power is not transmitted from the power transmission body 51 to the surface acoustic wave element 25, and the operation is stopped. And the reaction vessel 7 are held at a certain distance.
  • the arrangement determining member 52 operates under the control of the control unit 45 to move the power transmission body 51 during power transmission in which the reaction wheel 33 stops and power is transmitted from the power transmission body 51 to the surface acoustic wave element 25.
  • the position along the circumferential direction of the reaction wheel 33 is adjusted so that the power transmission body 51 and the reaction container 7 face each other, and the relative position between the position power transmission body 51 along the radial direction of the reaction wheel 33 and the reaction container 7 Determine placement.
  • the relative arrangement of the power transmission body 51 and the reaction container 7 is, for example, that a reflection sensor is provided on the power transmission body 51 side, and the reflector force provided at a specific location on the reaction container 7 or the surface acoustic wave element 25 is, for example, It is detected by using reflection. At this time, the detected relative arrangement data is input to the control unit 45.
  • the drive arm 27b is supported by the support cylinder 27a so as to be able to appear and retract.
  • an RF transmission antenna 21a is attached to a support member 27c provided inside.
  • the surface acoustic wave element 25 is attached to the tip of the arm member 57 with the transducer 25b facing outward and the antenna 25d facing inward.
  • the automatic analyzer 30 configured as described above operates under the control of the control unit 45 and rotates.
  • the reagent dispensing mechanisms 35 and 36 sequentially dispense the reagents from the reagent containers 31a and 32a to the plurality of reaction containers 7 conveyed along the circumferential direction by the reaction wheel 33.
  • specimens are sequentially dispensed from a plurality of specimen containers 39a held in the rack 39 by the specimen dispensing mechanism 41.
  • reaction container 7 into which the reagent and the sample have been dispensed is sequentially agitated by the agitator 50 to react the reagent and the sample, and the photometry is performed when the reaction wheel 33 rotates again. Pass through system 42. At this time, the liquid in the reaction container 7 is photometrically measured by the light receiving unit 42c, and the component concentration and the like are analyzed by the control unit 45. After the analysis, the reaction vessel 7 is washed by the washing mechanism 43 and then used again for analyzing the specimen.
  • the stirring device 50 is controlled from the nozzle 59 of the acoustic matching liquid dispensing mechanism as shown in FIG.
  • the acoustic matching liquid Lm is discharged to the acoustic wave element 25.
  • the stirring device 50 extends the drive arm 57b under the control of the control unit 45, and as shown in FIG. 22, the surface acoustic wave element 25 at the tip of the drive arm 57b is brought into contact with the side wall 7a of the reaction vessel 7.
  • the stirrer 50 generates the vibrator 25b of the surface acoustic wave element 25 through the thin film of the acoustic matching liquid Lm disposed between the surface acoustic wave element 25 and the side wall 7a.
  • Sound waves surface acoustic waves
  • the reaction vessel 7 flows into the liquid L due to the leaked sound wave (surface acoustic wave), and the directional force flow Fee in the obliquely upward direction of the transducer 25b and the directional force flow in the obliquely downward direction of the transducer 25b Few And the liquid is stirred.
  • the surface acoustic wave element 25 contacts the side wall 7a via the acoustic matching liquid Lm with the vibrator 25 directed toward the side wall 7a, and the sound wave (surface acoustic wave) is irradiated to the liquid L. Since the propagation path up to this point is short, the effects similar to those of the agitation device 20 of the first embodiment can be obtained, for example, the attenuation of sound waves can be suppressed and the liquid agitation efficiency can be improved.
  • the surface acoustic wave element 25 uses a comb-like electrode (IDT) as the vibrator 25b and is supplied with power wirelessly, so the structure is simple and the size is small.
  • IDT comb-like electrode
  • the agitator 50 and hence the automatic analyzer 30, can be miniaturized.
  • the stirring device 50 is driven under the control of the control unit 45.
  • the arm 57b is pulled back, and the contact between the surface acoustic wave element 25 and the side wall 7a is released.
  • the stirrer of the present invention is useful for improving the stirring efficiency of liquid because the propagation path of sound waves is short and the attenuation of sound waves is suppressed.
  • a living body such as blood or urine is used. Suitable for use in analyzers that analyze samples.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)

Abstract

La présente invention concerne un mélangeur comprenant un contenant recevant un liquide à mélanger et un moyen de génération d'onde sonore irradiant une onde sonore vers le liquide de manière à mélanger le liquide par l'onde sonore, ainsi qu'un analyseur. L'élément d'onde acoustique de surface (22) dans le mélangeur (20) comprend un substrat piézoélectrique (22a) ayant une surface de contact en contact avec le contenant (7), une partie de génération de son (22b) formée sur le substrat piézoélectrique qui génère l'onde sonore pour mélanger le liquide, ainsi qu'une partie de réception de puissance électrique (22c) formée sur la partie du substrat piézoélectrique autrement que sur la surface de contact et reçoit sans fil une puissance d'attaque pour la partie de génération de son fournie de l'extérieur.
PCT/JP2006/325637 2006-02-28 2006-12-22 Melangeur et analyseur Ceased WO2007099684A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2006053441A JP2007232523A (ja) 2006-02-28 2006-02-28 攪拌装置と分析装置
JP2006-053441 2006-02-28

Publications (1)

Publication Number Publication Date
WO2007099684A1 true WO2007099684A1 (fr) 2007-09-07

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JP (1) JP2007232523A (fr)
WO (1) WO2007099684A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009139237A (ja) * 2007-12-06 2009-06-25 Olympus Corp 反応容器
JP5485014B2 (ja) * 2010-05-14 2014-05-07 株式会社東芝 自動分析装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003509703A (ja) * 1999-09-16 2003-03-11 アドヴァリティクス アーゲー 高感度分解能検出装置及び方法
JP2003535349A (ja) * 2000-06-09 2003-11-25 アドヴァリティクス アーゲー 少量の物質を操作する装置及び方法
JP2004534633A (ja) * 2001-04-09 2004-11-18 アドヴァリティクス アーゲー 微量液体を混合するための混合方法、混合装置、その混合装置の使用方法及び表面付着力の分析方法
JP2005257406A (ja) * 2004-03-10 2005-09-22 Olympus Corp 液体攪拌デバイス

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003509703A (ja) * 1999-09-16 2003-03-11 アドヴァリティクス アーゲー 高感度分解能検出装置及び方法
JP2003535349A (ja) * 2000-06-09 2003-11-25 アドヴァリティクス アーゲー 少量の物質を操作する装置及び方法
JP2004534633A (ja) * 2001-04-09 2004-11-18 アドヴァリティクス アーゲー 微量液体を混合するための混合方法、混合装置、その混合装置の使用方法及び表面付着力の分析方法
JP2005257406A (ja) * 2004-03-10 2005-09-22 Olympus Corp 液体攪拌デバイス

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