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WO2007089038A8 - Novel phospholipase d - Google Patents

Novel phospholipase d

Info

Publication number
WO2007089038A8
WO2007089038A8 PCT/JP2007/052172 JP2007052172W WO2007089038A8 WO 2007089038 A8 WO2007089038 A8 WO 2007089038A8 JP 2007052172 W JP2007052172 W JP 2007052172W WO 2007089038 A8 WO2007089038 A8 WO 2007089038A8
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
acid sequence
phospholipase
ability
phosphatidylinositol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2007/052172
Other languages
French (fr)
Japanese (ja)
Other versions
WO2007089038A1 (en
Inventor
Yugo Iwasaki
Hideo Nakano
Tetsuya Takahashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nagoya University NUC
Original Assignee
Nagoya University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nagoya University NUC filed Critical Nagoya University NUC
Priority to JP2007556953A priority Critical patent/JP5156951B2/en
Publication of WO2007089038A1 publication Critical patent/WO2007089038A1/en
Publication of WO2007089038A8 publication Critical patent/WO2007089038A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6481—Phosphoglycerides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A modified phospholipase D having an ability to synthesize phosphatidylinositol which contains: (A) an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:2 by substitution of at least one of the amino acid residues at the 187-, 191- and 385-positions by other amino acid residue(s); or (B) an amino acid sequence of a polypeptide derived from the amino acid sequence as described in the above (A) by substitution, deletion, insertion and/or addition of at least one amino acid residue at a position different from the 187-, 191- and 385-positions and having an ability to synthesize phosphatidylinositol. By using thismodified phospholipase D, phosphatidylinositol can be efficiently produced from a phospholipid. It is also intended to provide a method of screening a phospholipase D having an ability to synthesize an acylglycerophospholipid having glycol group.
PCT/JP2007/052172 2006-02-02 2007-02-01 Novel phospholipase d Ceased WO2007089038A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2007556953A JP5156951B2 (en) 2006-02-02 2007-02-01 New phospholipase D

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2006-025550 2006-02-02
JP2006025550 2006-02-02

Publications (2)

Publication Number Publication Date
WO2007089038A1 WO2007089038A1 (en) 2007-08-09
WO2007089038A8 true WO2007089038A8 (en) 2008-05-15

Family

ID=38327588

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/052172 Ceased WO2007089038A1 (en) 2006-02-02 2007-02-01 Novel phospholipase d

Country Status (3)

Country Link
JP (2) JP5156951B2 (en)
CN (1) CN101379187A (en)
WO (1) WO2007089038A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102653778A (en) * 2012-05-08 2012-09-05 广东省微生物研究所 Enzymolysis preparation method of phosphatidylinositol
CN105734030A (en) * 2016-03-30 2016-07-06 中国海洋大学 Phospholipase D and application thereof
CN106085984B (en) * 2016-06-02 2019-07-19 天津科技大学 A novel phospholipase D and method for preparing phosphatidic acid and phosphatidylserine
CN107164397A (en) * 2017-04-28 2017-09-15 江南大学 The gene excavating and its application process of a kind of phospholipase D
WO2020133314A1 (en) * 2018-12-29 2020-07-02 邦泰生物工程(深圳)有限公司 Phospholipase d mutant, use thereof and method for preparing phosphatidylserine by means of same
CN115161303B (en) * 2019-11-21 2024-02-13 天津科技大学 Phospholipase mutant and method for synthesizing glycerophospholipids by using phospholipase mutant
CN112322602B (en) * 2020-11-03 2022-05-10 江南大学 A method for co-expressing phospholipase to promote the extracellular expression of proteins in Bacillus subtilis
CN116024195B (en) * 2022-12-28 2025-05-09 厦门蓝湾科技有限公司 A directed evolution method for improving the thermal stability of phospholipase D
CN116855474B (en) * 2023-06-12 2024-07-26 天津科技大学 A phospholipase mutant and its preparation and application
CN118895264B (en) * 2024-10-10 2024-12-10 中国海洋大学 Phospholipase D-Sp-256-A409C and its application

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06269287A (en) * 1993-03-19 1994-09-27 Kanegafuchi Chem Ind Co Ltd Production of phospholipid

Also Published As

Publication number Publication date
WO2007089038A1 (en) 2007-08-09
JP5156951B2 (en) 2013-03-06
JP2012187108A (en) 2012-10-04
JPWO2007089038A1 (en) 2009-06-25
CN101379187A (en) 2009-03-04
JP5420016B2 (en) 2014-02-19

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