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WO2007083100A3 - Enzyme - Google Patents

Enzyme Download PDF

Info

Publication number
WO2007083100A3
WO2007083100A3 PCT/GB2007/000124 GB2007000124W WO2007083100A3 WO 2007083100 A3 WO2007083100 A3 WO 2007083100A3 GB 2007000124 W GB2007000124 W GB 2007000124W WO 2007083100 A3 WO2007083100 A3 WO 2007083100A3
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
enzyme
residues
legumain
cleavage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2007/000124
Other languages
French (fr)
Other versions
WO2007083100A2 (en
Inventor
Vaskar Saha
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cancer Research Technology Ltd
Original Assignee
Cancer Research Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0600862A external-priority patent/GB0600862D0/en
Priority claimed from GB0618149A external-priority patent/GB0618149D0/en
Application filed by Cancer Research Technology Ltd filed Critical Cancer Research Technology Ltd
Publication of WO2007083100A2 publication Critical patent/WO2007083100A2/en
Publication of WO2007083100A3 publication Critical patent/WO2007083100A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A modified E. coli L-asparaginase enzyme that is resistant to cleavage by human asparaginyl endopeptidase (also known as legumain). The amino acid residues N46, D 146 and/or N 165 have been replaced with another amino acid residue, and preferably with glycine residues.
PCT/GB2007/000124 2006-01-17 2007-01-17 Enzyme Ceased WO2007083100A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0600862.7 2006-01-17
GB0600862A GB0600862D0 (en) 2006-01-17 2006-01-17 Enzyme
GB0618149A GB0618149D0 (en) 2006-09-15 2006-09-15 Enzyme II
GB0618149.9 2006-09-15

Publications (2)

Publication Number Publication Date
WO2007083100A2 WO2007083100A2 (en) 2007-07-26
WO2007083100A3 true WO2007083100A3 (en) 2007-11-08

Family

ID=37909467

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/000124 Ceased WO2007083100A2 (en) 2006-01-17 2007-01-17 Enzyme

Country Status (1)

Country Link
WO (1) WO2007083100A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012014228A1 (en) 2010-07-28 2012-02-02 Abhishek Narain Singh A method to by-pass allosteric domain activity of an enzyme so as to alter its feedback or feed-forward inhibition or activation
WO2012075173A2 (en) * 2010-12-01 2012-06-07 Board Of Regents The University Of Texas System Compositions and method for deimmunization of proteins
US20170089909A1 (en) * 2015-09-30 2017-03-30 Xiaohong Yu Methods and compositions for assaying blood levels of legumain
CN106556699B (en) 2015-09-30 2021-05-07 宁波紫园医疗器械有限公司 Methods and compositions for determining blood levels of legumain
EP3538652B1 (en) * 2016-11-08 2021-11-10 Universita' Degli Studi Di Pavia A highly stable, protease-resistant e. coli asparaginase
CN109694856B (en) * 2017-02-16 2020-02-04 李伟 Monoclonal antibody of anti-human Legumain protein, hybridoma cell strain LGMN-2 and application thereof
CN110331157B (en) * 2019-08-02 2021-06-08 湖北大学 A kind of fusion expression method of AEP cyclase in Escherichia coli, method for identifying the cyclization ability of AEP cyclase and its application
WO2022087589A1 (en) * 2020-10-20 2022-04-28 Flagship Pioneering Innovation Vi, Llc Asparaginase proteins

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005075675A2 (en) * 2004-02-03 2005-08-18 Bayer Healthcare Ag Diagnostics and therapeutics for diseases associated with putative cysteine protease 1 (prs1)

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005075675A2 (en) * 2004-02-03 2005-08-18 Bayer Healthcare Ag Diagnostics and therapeutics for diseases associated with putative cysteine protease 1 (prs1)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
PANOSYAN EDUARD H ET AL: "Pharmacokinetics and pharmacodynamics of three asparaginases in pediatric patients with higher risk acute lymphoblastic leukemia: A report from CCG-1961.", BLOOD, vol. 104, no. 11, Part 1, November 2004 (2004-11-01), & 46TH ANNUAL MEETING OF THE AMERICAN-SOCIETY-OF-HEMATOLOGY; SAN DIEGO, CA, USA; DECEMBER 04 -07, 2004, pages 751A, XP009082516, ISSN: 0006-4971 *
STAMS WENDY A G ET AL: "Asparagine synthetase expression is linked with L-asparaginase resistance in TEL-AML1-negative but not TEL-AML1-positive pediatric acute lymphoblastic leukemia.", BLOOD 1 JUN 2005, vol. 105, no. 11, 1 June 2005 (2005-06-01), pages 4223 - 4225, XP002429951, ISSN: 0006-4971 *
VERMA NEELAM ET AL: "L-asparaginase: a promising chemotherapeutic agent.", CRITICAL REVIEWS IN BIOTECHNOLOGY 2007 JAN-MAR, vol. 27, no. 1, January 2007 (2007-01-01), pages 45 - 62, XP009082474, ISSN: 0738-8551 *
ZHANG YU-QING ET AL: "Synthesis, characterization and immunogenicity of silk fibroin-L-asparaginase bioconjugates.", JOURNAL OF BIOTECHNOLOGY 21 NOV 2005, vol. 120, no. 3, 21 November 2005 (2005-11-21), pages 315 - 326, XP005136282, ISSN: 0168-1656 *

Also Published As

Publication number Publication date
WO2007083100A2 (en) 2007-07-26

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