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WO2007065697A3 - Selection marker system and method for screening a choline tolerant plant cell - Google Patents

Selection marker system and method for screening a choline tolerant plant cell Download PDF

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Publication number
WO2007065697A3
WO2007065697A3 PCT/EP2006/011801 EP2006011801W WO2007065697A3 WO 2007065697 A3 WO2007065697 A3 WO 2007065697A3 EP 2006011801 W EP2006011801 W EP 2006011801W WO 2007065697 A3 WO2007065697 A3 WO 2007065697A3
Authority
WO
WIPO (PCT)
Prior art keywords
choline
plant
selection marker
screening
wild
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2006/011801
Other languages
French (fr)
Other versions
WO2007065697B1 (en
WO2007065697A2 (en
Inventor
Pardha P Saradhi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Entelechon GmbH
Original Assignee
Entelechon GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Entelechon GmbH filed Critical Entelechon GmbH
Priority to US12/096,451 priority Critical patent/US8101410B2/en
Publication of WO2007065697A2 publication Critical patent/WO2007065697A2/en
Publication of WO2007065697A3 publication Critical patent/WO2007065697A3/en
Publication of WO2007065697B1 publication Critical patent/WO2007065697B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a selection marker system for distinguishing genetically modified plant cells from wild-type plant cells comprising (a) a nucleic acid sequence coding for choline oxidase, choline monooxygenase and/or choline dehydrogenase for genetically modifying at least a part of a plant and; (b) at least a part of a wild-type plant of the same plant species, wherein the part of the plant which is genetically modified by the nucleic acid sequence coding for choline oxidase, choline monooxygenase and/or choline dehydrogenase is capable of surviving in a medium containing choline at a concentration which is toxic to the wild- type plant. Furthermore, the invention relates to a method for screening and/or identifying a choline tolerant plant cell and the use of a nucleic sequence coding for a choline oxidase, choline monooxygenase and/or choline dehydrogenase as a selection marker for choline tolerance.
PCT/EP2006/011801 2005-12-08 2006-12-04 Selection marker system and method for screening a choline tolerant plant cell Ceased WO2007065697A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/096,451 US8101410B2 (en) 2005-12-08 2006-12-04 Selection marker system and method for screening a choline tolerant plant cell

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05090335A EP1795611B1 (en) 2005-12-08 2005-12-08 Selection marker system and method for screening a choline tolerant plant cell
EP05090335.0 2005-12-08

Publications (3)

Publication Number Publication Date
WO2007065697A2 WO2007065697A2 (en) 2007-06-14
WO2007065697A3 true WO2007065697A3 (en) 2007-10-04
WO2007065697B1 WO2007065697B1 (en) 2007-11-22

Family

ID=36580032

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/011801 Ceased WO2007065697A2 (en) 2005-12-08 2006-12-04 Selection marker system and method for screening a choline tolerant plant cell

Country Status (3)

Country Link
US (1) US8101410B2 (en)
EP (1) EP1795611B1 (en)
WO (1) WO2007065697A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101586526B1 (en) * 2012-03-26 2016-01-20 경상대학교산학협력단 Use of a gene encoding DAPG hydrolase as a selection marker for transgenic plant
CN111139311B (en) * 2020-01-17 2022-08-12 南京农业大学 A method for assessing the risk of weeding gene drift in transgenic rice and its application

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998026081A1 (en) * 1996-12-09 1998-06-18 Buelow Leif Transgenic plants having increased freezing and choline tolerance
WO1998030702A2 (en) * 1997-01-08 1998-07-16 University Of Florida Polynucleotides encoding choline monooxygenase and plants transformed therewith
US6756525B1 (en) * 1995-12-28 2004-06-29 Suntory Limited Method for producing temperature-tolerant plants

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ86798A3 (en) * 1996-06-21 1999-03-17 Monsanto Company Fertile transgenic wheat plant, process of its preparation, cells and seed of such a plant

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6756525B1 (en) * 1995-12-28 2004-06-29 Suntory Limited Method for producing temperature-tolerant plants
WO1998026081A1 (en) * 1996-12-09 1998-06-18 Buelow Leif Transgenic plants having increased freezing and choline tolerance
WO1998030702A2 (en) * 1997-01-08 1998-07-16 University Of Florida Polynucleotides encoding choline monooxygenase and plants transformed therewith

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GAWER M ET AL.: "Comparison between as choline-tolerant Nicotiana tabacum cell line and the corresponding wild type. I. Growth, thermosensitivity and lipid composition", PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 26, 1988, pages 323 - 331, XP008065411 *
LILIUS G ET AL: "ENHANCED NACL STRESS TOLERANCE IN TRANSGENIC TOBACCO EXPRESSING BACTERIAL CHOLINE DEHYDROGENASE", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 14, no. 2, 14 February 1996 (1996-02-14), pages 177 - 180, XP002062844, ISSN: 1087-0156 *
PRASAD K V S K ET AL.: "Enhanced tolerance of transgenic Brassica juncea to choline confirms successful expression of the bacterial codA gene", PLANT SCIENCE, vol. 159, 2000, pages 233 - 242, XP002386177 *
YILMAZ J L AND BÜLOW L: "Enhanced stress tolerance in Escherichia coli and Nicotiana tabacum expressing a betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein", BIOTECHNOLOGY PROGRESS, vol. 18, 2002, pages 1176 - 1182, XP002386178 *

Also Published As

Publication number Publication date
WO2007065697B1 (en) 2007-11-22
WO2007065697A2 (en) 2007-06-14
US8101410B2 (en) 2012-01-24
US20090170093A1 (en) 2009-07-02
EP1795611B1 (en) 2012-05-02
EP1795611A1 (en) 2007-06-13

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