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WO2007051459A2 - Procede pour enrichir selectivement des biomolecules ou des bioparticules par liaison particulaire - Google Patents

Procede pour enrichir selectivement des biomolecules ou des bioparticules par liaison particulaire Download PDF

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Publication number
WO2007051459A2
WO2007051459A2 PCT/DE2006/001931 DE2006001931W WO2007051459A2 WO 2007051459 A2 WO2007051459 A2 WO 2007051459A2 DE 2006001931 W DE2006001931 W DE 2006001931W WO 2007051459 A2 WO2007051459 A2 WO 2007051459A2
Authority
WO
WIPO (PCT)
Prior art keywords
biomolecules
binding
bound
target molecule
particle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2006/001931
Other languages
German (de)
English (en)
Other versions
WO2007051459A3 (fr
Inventor
Dieter Scholz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2007051459A2 publication Critical patent/WO2007051459A2/fr
Publication of WO2007051459A3 publication Critical patent/WO2007051459A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/5432—Liposomes or microcapsules

Definitions

  • the invention relates to the use of immobilized micro-beads for the enrichment of biomolecules and bioparticles.
  • Fields of application of the invention are biotechnology, medicine and molecular biology research.
  • FACS fluorescence activated cell sorting
  • Magnetic separation processes have been used more and more for more than 10 years to detect and isolate particles / cells.
  • the separation is carried out by a strong magnetic field.
  • the immuno-magnetic separation successfully commercialized by, among others, Dynal and Miltenyi Biotec, has become established as a simple, relatively inexpensive method of cell separation.
  • the magnetic separation has proven itself for the isolation of relatively rare cell types, eg for the isolation of fetal cells from the maternal blood for prenatal diagnosis.
  • Another application is the detection of tumor cells in the blood after surgical removal of the primary tumor to initiate further treatments.
  • syngeneic CD34 + peripheral blood stem cells are now routinely obtained from patients for reimplantation suffering from certain malignancies of the blood-forming system. This is preferably done by the purification of leukocytes with specific antibodies coupled to magnetic beads. For cell fractionation during the blood / cell donation several systems are available that use the different size and specific gravity of the blood cells for a separation in the gravitational field (centrifugation).
  • PCT / DE 001371 describes a process of cascade filtration with which several substances to be separated from mixtures or large volumes are separated / enriched in one operation by using microparticles of different sizes to which different specific "catcher" molecules are coupled are.
  • the invention has for its object to develop a method with the small amounts of substances to be detected are bound to small surfaces, which are then available for further analytical or other purposes.
  • This object has been achieved by attaching a second capture principle to the microbead surface without or via a spacer which binds the complex microparticle biomolecule to a surface.
  • a second capture principle to the microbead surface without or via a spacer which binds the complex microparticle biomolecule to a surface.
  • As catcher principle everything that is useful for temporary or permanent fixation, usually biotin-avidin complex or even antibody antigen or molecule interactions with sufficiently large adhesion (eg via hydrophobic interactions) is suitable.
  • the combination of cascade filtration with the described microparticle fixation provides an easy-to-use system with which small amounts of a target substance are permanently or temporarily fixed from substance mixtures for further processing.
  • the target biomolecule can also be detached from the complex carrier-microbead target molecule by other chemical or physical effects.
  • the method is suitable, inter alia, for detection systems of biomolecules in liquids such as blood, excretions and secrete but also in food, environment and the process control of biotechnological production processes.
  • liquids such as blood, excretions and secrete but also in food, environment and the process control of biotechnological production processes.
  • the complex microparticle biomolecule is immobilized on a solid surface for further work steps.

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

L'invention concerne l'utilisation de microbilles immobilisées pour enrichir des biomolécules et des bioparticules. Cette invention se rapporte en outre à un procédé pour enrichir sélectivement des biomolécules ou des bioparticules, par liaison particulaire, à l'aide d'au moins deux procédés de liaison différents. Les domaines d'application de cette invention sont la biotechnologie, la médecine, et la recherche en biologie moléculaire.
PCT/DE2006/001931 2005-11-03 2006-11-03 Procede pour enrichir selectivement des biomolecules ou des bioparticules par liaison particulaire Ceased WO2007051459A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102005052957.7 2005-11-03
DE200510052957 DE102005052957A1 (de) 2005-11-03 2005-11-03 Verfahren zur selektiven partikelgebundenen Anreicherung von Biomolekülen oder Biopartikeln

Publications (2)

Publication Number Publication Date
WO2007051459A2 true WO2007051459A2 (fr) 2007-05-10
WO2007051459A3 WO2007051459A3 (fr) 2007-07-26

Family

ID=37885829

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2006/001931 Ceased WO2007051459A2 (fr) 2005-11-03 2006-11-03 Procede pour enrichir selectivement des biomolecules ou des bioparticules par liaison particulaire

Country Status (2)

Country Link
DE (1) DE102005052957A1 (fr)
WO (1) WO2007051459A2 (fr)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1312277C (fr) * 1987-12-18 1993-01-05 Richard C. Sutton Reactifs immobilises par l'avidine et la biotine, elements d'analyse et methodes d'utilisation
FI20002623A7 (fi) * 2000-11-30 2002-05-31 Inno Trac Diagnostics Oy Bioanalyyttinen määritysmenetelmä
JP4087200B2 (ja) * 2002-09-17 2008-05-21 ユニバーサル・バイオ・リサーチ株式会社 粒子複合体及び該粒子複合体の作製方法
GB0228914D0 (en) * 2002-12-11 2003-01-15 Dynal Biotech Asa Particles

Also Published As

Publication number Publication date
DE102005052957A1 (de) 2007-05-10
WO2007051459A3 (fr) 2007-07-26

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Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
WA Withdrawal of international application