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WO2007050715A3 - Compositions and methods for safe delivery of biologically-active plant transformation agents using non-fibrous silicon carbide powder - Google Patents

Compositions and methods for safe delivery of biologically-active plant transformation agents using non-fibrous silicon carbide powder Download PDF

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Publication number
WO2007050715A3
WO2007050715A3 PCT/US2006/041702 US2006041702W WO2007050715A3 WO 2007050715 A3 WO2007050715 A3 WO 2007050715A3 US 2006041702 W US2006041702 W US 2006041702W WO 2007050715 A3 WO2007050715 A3 WO 2007050715A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
novel
biologically
impart
conditions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/041702
Other languages
French (fr)
Other versions
WO2007050715A2 (en
Inventor
Joseph D Reddy
Dean W Gabriel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Integrated Plant Genetics Inc
Original Assignee
Integrated Plant Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Integrated Plant Genetics Inc filed Critical Integrated Plant Genetics Inc
Publication of WO2007050715A2 publication Critical patent/WO2007050715A2/en
Publication of WO2007050715A3 publication Critical patent/WO2007050715A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

According to the described invention, a biologically-active nucleic acid may be directly delivered into plant cells in an improved and operator-safe fashion using encapsulated or complexed nucleic acid formed into nanoparticles, using carborundum powder, a nucleic acid binding protein with nuclear localization signals (NLSs), or both. The plant cells are not enzymatically treated, and the delivery is not mediated by any microbial agent. The present invention provides the following: (1) a nucleic acid delivery system (NADS), i.e. macromolecular nanoparticles to impart the novel conditions; (2) a novel carrier medium (CM) used with the nucleic acid delivery system which impart the desired results; (3) novel, specific conditions that impart the desired or improved efficiency of the transformation system into the plant cell without irreversibly damaging it, and (4) a novel method to produce these conditions through the use of a combination of DNA condensing agents, carborundum powder, a nucleic acid binding protein with NLSs, which together imparts improved nucleic acid delivery efficiency and safety as compared to other known systems.
PCT/US2006/041702 2005-10-26 2006-10-26 Compositions and methods for safe delivery of biologically-active plant transformation agents using non-fibrous silicon carbide powder Ceased WO2007050715A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59685205P 2005-10-26 2005-10-26
US60/596,852 2005-10-26

Publications (2)

Publication Number Publication Date
WO2007050715A2 WO2007050715A2 (en) 2007-05-03
WO2007050715A3 true WO2007050715A3 (en) 2007-11-29

Family

ID=37968538

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/041702 Ceased WO2007050715A2 (en) 2005-10-26 2006-10-26 Compositions and methods for safe delivery of biologically-active plant transformation agents using non-fibrous silicon carbide powder

Country Status (1)

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WO (1) WO2007050715A2 (en)

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CA2615797C (en) 2005-07-18 2014-04-29 Pioneer Hi-Bred International, Inc. Modified frt recombination sites and methods of use
WO2009046384A1 (en) * 2007-10-05 2009-04-09 Dow Agrosciences Llc Methods for transferring molecular substances into plant cells
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
TWI576432B (en) * 2009-04-07 2017-04-01 陶氏農業科學公司 Nanoparticle mediated delivery of sequence specific nucleases
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
DK3231872T3 (en) 2010-03-08 2020-07-20 Monsanto Technology Llc POLYNUCLEOTIDE MOLECULES FOR PLANT REGENEULATION
EP2418283A1 (en) * 2010-08-07 2012-02-15 Nomad Bioscience GmbH Process of transfecting plants
MX350771B (en) * 2011-09-13 2017-09-15 Monsanto Technology Llc Methods and compositions for weed control.
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
IN2014MN02404A (en) 2012-05-24 2015-08-21 Seeds Ltd Ab
UY35251A (en) 2013-01-01 2014-07-31 Seeds Ltd Ab ISOLATED DsRNA MOLECULES AND METHODS TO USE THEM TO SILENCE DIANA OF INTEREST MOLECULES
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
CA2905027A1 (en) 2013-03-13 2014-10-09 Monsanto Technology Llc Methods and compositions for weed control
MX2015012334A (en) 2013-03-13 2016-02-05 Monsanto Technology Llc Methods and compositions for weed control.
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
JP6668236B2 (en) 2013-07-19 2020-03-18 モンサント テクノロジー エルエルシー Composition for controlling LEPTINOTARSA and method therefor
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
US9540642B2 (en) 2013-11-04 2017-01-10 The United States Of America, As Represented By The Secretary Of Agriculture Compositions and methods for controlling arthropod parasite and pest infestations
UA119253C2 (en) 2013-12-10 2019-05-27 Біолоджикс, Інк. METHOD FOR VARROA TREATMENT AND VEGETABLES
CN105979770B (en) 2014-01-15 2019-07-05 孟山都技术公司 Methods and compositions for weed control using EPSPS polynucleotides
CN106413390B (en) 2014-04-01 2019-09-27 孟山都技术公司 Compositions and methods for controlling pests
EP3158067B1 (en) 2014-06-23 2020-08-12 Monsanto Technology LLC Compositions and methods for regulating gene expression via rna interference
US11807857B2 (en) 2014-06-25 2023-11-07 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
AR101348A1 (en) 2014-07-29 2016-12-14 Monsanto Technology Llc COMPOSITIONS AND METHODS FOR PEST CONTROL BY INSECTS
US10968449B2 (en) 2015-01-22 2021-04-06 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
CN107750125A (en) * 2015-06-02 2018-03-02 孟山都技术有限公司 Compositions and methods for delivering polynucleotides to plants
AU2016270913A1 (en) 2015-06-03 2018-01-04 Monsanto Technology Llc Methods and compositions for introducing nucleic acids into plants

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5302523A (en) * 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5693512A (en) * 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US5831020A (en) * 1997-03-25 1998-11-03 The Research Foundation Of State University Of New York Protein-mediated nuclear import of DNA

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5302523A (en) * 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5464765A (en) * 1989-06-21 1995-11-07 Zeneca Limited Transformation of plant cells
US5693512A (en) * 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US5831020A (en) * 1997-03-25 1998-11-03 The Research Foundation Of State University Of New York Protein-mediated nuclear import of DNA

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FINER ET AL.: "Use of Agrobacterium expressing green fluorescent protein to evaluate colonization of sonication-assisted Agrobacteriummediated transformation-treated soybean cotyledons", LETTERS IN APPLIED MICROBIOLOGY, vol. 30, 2000, pages 406 - 410 *
GIETL ET AL.: "Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single-stranded DNA", PROC. NATL. ACAD. SCI. USA, vol. 84, December 1987 (1987-12-01), pages 9006 - 9010 *

Also Published As

Publication number Publication date
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