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WO2007050100A3 - Immobilized enzymes and processes for preparing and using same - Google Patents

Immobilized enzymes and processes for preparing and using same Download PDF

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Publication number
WO2007050100A3
WO2007050100A3 PCT/US2005/042657 US2005042657W WO2007050100A3 WO 2007050100 A3 WO2007050100 A3 WO 2007050100A3 US 2005042657 W US2005042657 W US 2005042657W WO 2007050100 A3 WO2007050100 A3 WO 2007050100A3
Authority
WO
WIPO (PCT)
Prior art keywords
silica gel
immobilized
enzyme
modified silica
aminoalkylsilane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/042657
Other languages
French (fr)
Other versions
WO2007050100A9 (en
WO2007050100A2 (en
Inventor
Allan David
Arthur Yang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Industrial Science and Technology Network Inc
Original Assignee
Industrial Science and Technology Network Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Industrial Science and Technology Network Inc filed Critical Industrial Science and Technology Network Inc
Publication of WO2007050100A2 publication Critical patent/WO2007050100A2/en
Anticipated expiration legal-status Critical
Publication of WO2007050100A9 publication Critical patent/WO2007050100A9/en
Publication of WO2007050100A3 publication Critical patent/WO2007050100A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

A highly loaded immobilized enzyme conjugate is prepared from a nanopore silica gel support. The enzyme may be immobilized in an amount, based on the silica gel support, of at least about 500 mg enzyme per gram of silica gel. The immobilized enzyme conjugate may be prepared by a method which includes the following steps: (a) surface modifying silicic acid by reacting the silicic acid with a basic aminoalkylsilane in solution in an alcoholic medium, wherein the amount of the basic aminoalkylsilane is sufficient to raise the pH of the solution to induce gelation and form a chemically surface-modified silica gel; (b) activating the chemically surface-modified silica gel by reaction with a cross-linking agent; and (c) contacting the activated chemically surface-modified silica gel with an enzyme. Immobilized thermolysin has been used to prepare a low molecular weight protamine with high efficiency.
PCT/US2005/042657 2004-11-24 2005-11-23 Immobilized enzymes and processes for preparing and using same Ceased WO2007050100A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63021304P 2004-11-24 2004-11-24
US60/630,213 2004-11-24

Publications (3)

Publication Number Publication Date
WO2007050100A2 WO2007050100A2 (en) 2007-05-03
WO2007050100A9 WO2007050100A9 (en) 2007-06-14
WO2007050100A3 true WO2007050100A3 (en) 2009-05-22

Family

ID=37968249

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/042657 Ceased WO2007050100A2 (en) 2004-11-24 2005-11-23 Immobilized enzymes and processes for preparing and using same

Country Status (2)

Country Link
TW (1) TW200630489A (en)
WO (1) WO2007050100A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101632900B (en) * 2009-08-11 2012-06-27 孙祥章 High-efficiency gas purification bioreactor based on complex enzyme immobilization technology and purification method thereof
KR101644939B1 (en) * 2014-04-29 2016-08-12 재단법인 전라북도생물산업진흥원 Method for immobilization of enzyme and immobilized enzyme using the method
WO2016065282A1 (en) * 2014-10-24 2016-04-28 The Regents Of The University Of Michigan Nasal formulation, nasal kit, and method for enhancing nasal nitric oxide (no) levels
US10669153B2 (en) * 2017-05-23 2020-06-02 International Business Machines Corporation Neuro-chemical sensor with selectively permeable membrane on nano-electrode
CN112888781A (en) * 2018-07-20 2021-06-01 路易斯·菲利普·艾豪·德·安德拉德 Composite material for stabilizing active biological material, method for producing same and use thereof
US20220411537A1 (en) * 2020-05-06 2022-12-29 Universidad De Los Andes Bio-nanocompound as an agent for nucleating aqueous-based compounds and production method thereof
CN113234716B (en) * 2021-05-28 2023-09-29 华南理工大学 A method of treating immobilized enzymes using strengthening liquid and its application
CN114438072B (en) * 2022-04-08 2022-05-31 山东天力药业有限公司 Production method of trehalose

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3519538A (en) * 1968-09-05 1970-07-07 Corning Glass Works Chemically coupled enzymes
US4323650A (en) * 1974-06-25 1982-04-06 United Kingdom Atomic Energy Authority Immobilization of biologically active substances in a porous support
US4506015A (en) * 1980-05-08 1985-03-19 Borden Company Limited Multi-layer immobilized enzyme compositions
US6042824A (en) * 1996-05-24 2000-03-28 Altus Biologics, Inc. Methods using cross linked protein crystal formulations as catalysts in organic solvents
US6303290B1 (en) * 2000-09-13 2001-10-16 The Trustees Of The University Of Pennsylvania Encapsulation of biomaterials in porous glass-like matrices prepared via an aqueous colloidal sol-gel process

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3519538A (en) * 1968-09-05 1970-07-07 Corning Glass Works Chemically coupled enzymes
US4323650A (en) * 1974-06-25 1982-04-06 United Kingdom Atomic Energy Authority Immobilization of biologically active substances in a porous support
US4506015A (en) * 1980-05-08 1985-03-19 Borden Company Limited Multi-layer immobilized enzyme compositions
US6042824A (en) * 1996-05-24 2000-03-28 Altus Biologics, Inc. Methods using cross linked protein crystal formulations as catalysts in organic solvents
US6303290B1 (en) * 2000-09-13 2001-10-16 The Trustees Of The University Of Pennsylvania Encapsulation of biomaterials in porous glass-like matrices prepared via an aqueous colloidal sol-gel process

Also Published As

Publication number Publication date
WO2007050100A9 (en) 2007-06-14
TW200630489A (en) 2006-09-01
WO2007050100A2 (en) 2007-05-03

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