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WO2006130470A3 - Methods and compositions comprising chimeric and full-length human acetyl-coa carboxylase - Google Patents

Methods and compositions comprising chimeric and full-length human acetyl-coa carboxylase Download PDF

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Publication number
WO2006130470A3
WO2006130470A3 PCT/US2006/020461 US2006020461W WO2006130470A3 WO 2006130470 A3 WO2006130470 A3 WO 2006130470A3 US 2006020461 W US2006020461 W US 2006020461W WO 2006130470 A3 WO2006130470 A3 WO 2006130470A3
Authority
WO
WIPO (PCT)
Prior art keywords
acid sequence
amino acid
human
yeast cell
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/020461
Other languages
French (fr)
Other versions
WO2006130470A2 (en
Inventor
Robert Haselkorn
Piotr Gornicki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Chicago
Original Assignee
University of Chicago
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Chicago filed Critical University of Chicago
Publication of WO2006130470A2 publication Critical patent/WO2006130470A2/en
Anticipated expiration legal-status Critical
Publication of WO2006130470A3 publication Critical patent/WO2006130470A3/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Disclosed are isolated nucleic acid sequences encoding and conjugates comprising a non-human acetyl-CoA carboxylase (ACC) amino acid sequence and a human ACC amino acid sequence. Yeast cells comprising a nucleic acid sequence encoding a non-human ACC amino acid sequence and a human ACC amino acid sequence are also disclosed. Also disclosed are methods that involve (a) obtaining a candidate substance; (b) preparing a first and second yeast cell, wherein the first and second yeast cells each comprise a nucleic acid sequence encoding a non-human ACC amino acid sequence and a human ACC amino acid sequence; (c) contacting the first yeast cell with the candidate substance; and (d) assaying for growth inhibition of the first yeast cell by comparing the growth of the first yeast cell in the presence of the candidate substance to growth of the second yeast cell in the absence of the candidate substance. These methods can be applied, for example, in the identification of modulators of human ACC which can be directed to the treatment and prevention of diseases such as obesity, cardiovascular disease, diabetes, insulin resistance, hyperproliferative disease, and metabolic disease.
PCT/US2006/020461 2005-05-27 2006-05-26 Methods and compositions comprising chimeric and full-length human acetyl-coa carboxylase Ceased WO2006130470A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68528305P 2005-05-27 2005-05-27
US60/685,283 2005-05-27

Publications (2)

Publication Number Publication Date
WO2006130470A2 WO2006130470A2 (en) 2006-12-07
WO2006130470A3 true WO2006130470A3 (en) 2007-12-06

Family

ID=37482177

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/020461 Ceased WO2006130470A2 (en) 2005-05-27 2006-05-26 Methods and compositions comprising chimeric and full-length human acetyl-coa carboxylase

Country Status (1)

Country Link
WO (1) WO2006130470A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306636B1 (en) * 1997-09-19 2001-10-23 Arch Development Corporation Nucleic acid segments encoding wheat acetyl-CoA carboxylase
US7211423B2 (en) * 2004-07-23 2007-05-01 Bristol-Myers Squibb Co. Acetyl CoA carboxylase 2 sequences and methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306636B1 (en) * 1997-09-19 2001-10-23 Arch Development Corporation Nucleic acid segments encoding wheat acetyl-CoA carboxylase
US7211423B2 (en) * 2004-07-23 2007-05-01 Bristol-Myers Squibb Co. Acetyl CoA carboxylase 2 sequences and methods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ABU-ELHEIGA ET AL.: "Human Acetyl-CoA Carboxylase", J. BIOL. CHEM., vol. 272, no. 16, 18 April 1997 (1997-04-18), pages 10669 - 10677, XP002952090 *

Also Published As

Publication number Publication date
WO2006130470A2 (en) 2006-12-07

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