WO2006126488A1 - Cauliflower mushroom extract - Google Patents

Abstract

Disclosed are a relatively low-molecular weight anti-tumor substance having an excellent pharmacological activity and can be absorbed readily through the digestive tract, and an anti-tumor agent, a cosmetic and a health food comprising the substance as an active ingredient. A cauliflower mushroom extract composition comprising, as the main ingredient, a substance having a molecular weight of 500 to 10000, the substance being produced by freeze-drying a fruiting body or mycelium of a cauliflower mushroom (Sparassis crispa), dividing the dried material into a fine powder, extracting the powder with a solvent and fractionating the extract; and an anti-tumor composition, a cosmetic and a health food comprising the cauliflower mushroom extract composition as an active ingredient.

Description

 Specification

 Hanabira bamboo extract

 Technical field

 [0001] The present invention relates to an extract of Hanabira bamboo mainly composed of a relatively low molecular weight component obtained from the fruit body or mycelia of Hanabiratake, and an antitumor agent, cosmetic and health food containing the same as an active ingredient .

 Background art

 [0002] Mushrooms have been used for food since ancient times, but in recent years, the physiological activity of the components has been clarified. Among them, krestin, lentinan, schizophyllan (all trade names) are recognized as useful as pharmaceuticals, and agaritas, mesimacob, ganoderma, etc. are expected to have an immunostimulatory effect and an antitumor effect. Attempts have been made to use dried or extracted substances or mycelia as health food ingredients. For example, Mannentake has been tried to use fruit bodies or extracts thereof as raw materials for health foods (see, for example, Patent Documents 1, 2, and 3).

[0003] On the other hand, Hanabiratake (Sparassis crispa), which belongs to these mushrooms, is a mushroom that grows in larch and the like, and is characterized by its pure white color that is crunchy and its shape like leaf peony. It is an edible mushroom. Up until now, it has been said that this bamboo shoot has slow growth and is extremely difficult to cultivate artificially. However, in recent years, a new cultivation method that can be cultivated in a relatively short period of time has been established and can be supplied on a commercial scale. (For example, see Patent Documents 4 and 5).

 [0004] It has been clarified so far that the fruiting body of the garlic bamboo has antitumor activity (see, for example, Non-Patent Documents 1 and 2). The active ingredient is said to be | 8-1,3-glucan, which accounts for about 40 to 50% of the dry weight of Hanabiratake (for example, see Patent Document 6).

 Patent Document 1: Japanese Patent Laid-Open No. 2001-10970

 Patent Document 2: Japanese Patent Laid-Open No. 2001-131083

Patent Document 3: Japanese Patent Laid-Open No. 2003-183176 Patent Document 4: Japanese Patent Laid-Open No. 11-56098

 Patent Document 5: Japanese Patent Laid-Open No. 2002-369621

 Non-Patent Document 1: Biol. Pharm. Bull. 23 (7) 866-872 (2000)

 Non-Patent Document 2: Biol. Pharm. Bull. 25 (7) 931—939 (2002)

 Patent Document 6: Japanese Unexamined Patent Publication No. 2000-217543

 Disclosure of the invention

 Problems to be solved by the invention

[0005] β-glucan, which is considered to be the main component of the antitumor activity of mushrooms, has a molecular weight of about several millions, and generally cannot necessarily be absorbed from the digestive tract. Therefore

Therefore, an antitumor component having a lower molecular weight and good absorption of gastrointestinal tract has been strongly desired.

[0006] An object of the present invention is to provide a low molecular weight antitumor component derived from the fruit body or mycelium of Hanabiratake and an antitumor agent, a cosmetic and a health food containing the same as an active ingredient.

 Means for solving the problem

 [0007] As a result of intensive studies in order to achieve the above-mentioned problems, the present inventors have obtained a low molecular weight component containing almost no β-glucan obtained from the fruit body of mycelia or mycelium. It has been found that it has antitumor activity, and has led to the present invention.

 [0008] That is, the present invention is a solvent extract of Hanabiratake, the main component is a molecular weight of 500 ~

The gist of the extract of the flower is a component within the range of 10,000, and another invention is a component of the solvent extract of the flower with a molecular weight of 500 to 1000 using a dialysis membrane. In another invention, a component having a molecular weight in the range of 500 to 10,000 is separated from the solvent extract of Hanabiratake by gel filtration. In another invention, the main component is a component having a molecular weight in the range of 500 to 10000 by performing ethanol precipitation from a solvent extract of Hanabiratake. The gist of the extract is a garlic bamboo extract obtained by fractionation. Further, another invention is characterized by an antitumor agent containing the above-mentioned extract of nobira and nabiratake as an active ingredient, and another invention provides a cosmetic containing the above-mentioned extract of vanilla bamboo as an active ingredient. It is intended as a summary Still another invention is summarized as a health food containing the above-mentioned Hanabiratake extract as an active ingredient.

 The present invention also provides a method for treating tumors or a method for inhibiting the growth of tumor cells by administering the above-mentioned solvent extract of Hanabiratake to a subject, and further comprising the solvent extract of Hanabiratake for the production of an antitumor agent. Also provides use.

 The invention's effect

 [0009] According to the present invention, since it is a low molecular weight component having a molecular weight in the range of 500 to 10,000, it is excellent in absorption of gastrointestinal strength when administered orally, and as a result, has an effect of improving antitumor activity. .

 Brief Description of Drawings

 [0010] FIG. 1 is a graph showing the molecular weight distribution (analysis result by GPC method) of the fruit extract of the present invention (hot water extract and low molecular weight antitumor component).

 FIG. 2 is a graph showing the results of a tumor growth inhibition test by oral ingestion of the extract of the present invention (the change in tumor size over time).

 BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the present invention will be described in detail.

 [0012] Hanabiratake is a mushroom that occurs specifically in coniferous forests such as larch at an altitude of 1000 meters or higher, and has been called a “phantom mushroom” because it is difficult to find. Until now, the cultivation has been difficult and generally not well known, but in recent years, artificial cultivation methods have been established and mass production has become possible.

 [0013] The fruit body used in the present invention is not particularly limited in its production method, and may be a natural product or a product obtained by artificial cultivation. Artificial cultivation can be carried out by preparing a conventionally known bacterial bed for artificial cultivation (for details, see JP-A-11-56098, JP-A-2002-369621 and JP-A-2002-369621). 2002-125460, US Pat. No. 6,212,822, etc.).

[0014] In addition, the mycelium mycelium used in the present invention can be obtained by a conventional liquid culture method. As the carbon source used in the medium, a commonly used carbon source such as dextrin and glycerol can be used in addition to a monosaccharide such as glucose. Inorganic or nitrogen sources Can use an organic nitrogen source, but it is preferable to use an organic nitrogen source in terms of growth rate. In addition, adding growth factors such as trace elements and vitamins as needed is no different from normal culture. The culture temperature is preferably 15 ° to 30 °°, and 11 is preferably 2.5 to 8.0. It is preferable to add an insoluble component to the medium component because it can grow uniformly. The culture period can be set to several days or several weeks depending on the strain.

[0015] The fruit body, Nabiratake fruit body or mycelium thus obtained is transferred to the next extraction step.

1S The form of the bamboo shoot is not particularly limited, and may be as it is, or may be a freeze-dried product or a fine powder of a dried product. Of these, a dry powder of fine powder is preferable from the viewpoint of extraction efficiency.

 [0016] Examples of the extraction solvent used in the extraction step include water, methanol, ethanol, isopropyl alcohol, butanol, glycerin, ethylene glycol, 1,3-butylene glycol, acetone, black mouth form, ethyl acetate, hexane, ether, and the like. Can be used. Among these, hot water is more preferable even though water is preferred. The extraction method using hot water is not particularly limited. For example, add 5 to LOO times the amount of water to the dried powder of the fruit body or mycelium, and add 80 to 100 ° C (100 to 121 under high pressure conditions). Boil (heat) for 20 minutes to 3 hours at ° C), or add 5 to LOO times the amount of water to the dry powder and heat to reflux for 1 to 3 hours. Any method may be used such as repeating the hot water extraction treatment several times.

 [0017] Since the solvent extract thus obtained contains components having a molecular weight in the range of 500 to 10,000, the low molecular weight components may be fractionated from the solvent extract. The method of fractionation is not particularly limited, and examples thereof include a method using a dialysis membrane, a gel filtration method or a method using ethanol precipitation. Specifically, it can be performed as follows.

[0018] The solvent extract obtained as described above is made into a dried product by freeze-drying treatment, and then redissolved in an appropriate amount of water so that the molecular weight cut-off is in the range of 5,000 to 10,000. Dialysis is performed using a certain dialysis membrane. Specific examples of dialysis membranes include Funakoshi, Spectra Biotech membrane series, Spectra Z-pore CE series, and RC series. Distilled water may be used for the external dialysis solution, but sodium acetate, sodium hydroxide, urea, etc. are appropriately added to the dialysis internal solution prepared from the dried solvent extract depending on the solubility of the dried product. . Fill a container containing dialysis solution (distilled water) with dialysis solution and immerse a membrane (tube) tightly sealed at both ends and leave it for 1 to 3 days or under stirring. As a result, a component having a molecular weight in the range of 500 to 10,000 is obtained in the permeation liquid. It is also possible to increase the recovered amount of the dialysis liquid fraction by repeating the dialysis step several times.

[0019] In the gel filtration method, the dried product of the solvent extract obtained in the same manner as described above is dissolved in an appropriate concentration, and passed through a glass column packed with a gel filtration carrier, so that the molecular weight is reduced. Components in the range of 500 to 10,000 can be obtained. Examples of the column carrier used here include Cefacryl series and Cefadex series manufactured by Amersham Almacia Biotech.

 [0020] In the ethanol precipitation method, the solvent extract obtained in the same manner as described above is dissolved in 75% ethanol, and the insoluble precipitate is removed to obtain a molecular weight of 500 to 10,000 in the supernatant. Components in the range can be obtained.

 [0021] The molecular weight obtained in this way is in the range of 500 to 10,000, preferably in the range of 20000 to 8000, more preferably in the range of 4,000 to 6000, and most preferably As shown in the following examples, it has been clarified that the extract of the chanterelle mushroom, which is mainly composed of a component having a molecular weight of about 5000, has a high antitumor activity. This is thought to be due to the fact that the absorption of gastrointestinal force was promoted due to the low molecular weight of this component, or that the immune activity in the intestinal tract was improved.

 In the present invention, the molecular weight is determined by the GPC method (column; UltrahydrogelGuard + 120 + 500; manufactured by Waters, mobile phase; 0.5M phosphate buffer (pHll), flow rate; 0.5mLZ, detection; differential refractive index, estimation The molecular weight is calculated from the retention time of dextran of each molecular weight).

 [0022] The component having a molecular weight in the range of 500 to 10000 is preferably 80 to 100% by weight, more preferably 90 to 100% by weight, most preferably, in the dry weight of the extract of the present invention. Is 95% to 100% by weight.

In addition, the garlic bamboo extract composition of the present invention hardly contains β-glucan having a molecular weight of about several millions, and the dry weight of the garlic bamboo extract composition of the present invention contains j8 glucan having a molecular weight of about several millions. The amount is 5% by weight or less, preferably 1% by weight or less The

 The antitumor composition, antitumor agent, cosmetic product or health food of the present invention contains the extract of the bamboo shoot obtained as described above as an active ingredient, and further, a pharmaceutically acceptable carrier or cosmetic. Containing an acceptable carrier or a food acceptable as a health food and / or a beverage. The content of the extract is not particularly limited because it is an extremely safe mushroom with food experience, but the lower limit is generally an amount that exhibits an effect according to the purpose of prevention or treatment. The upper limit is easy-to-use, economic power, and other viewpoints. Based on the actual amount, 5 mg to 500 g per day for adults, converted to the dry weight of the fruit body or mycelium that is the raw material, Preferably, the content and intake amount may be set so that 50 mg to 50 g can be ingested. For the composition of the present invention, the dosage is such that the adult can take 0.25 mg to 2.5 g, preferably 2.5 mg to 2.5 g per day, in terms of dry weight. What is necessary is just to set intake. The form of the antitumor agent, cosmetic or health food of the present invention is not particularly limited, and is in any form such as tablet, capsule, powder, granule, liquid, suspension, cream, etc. Available.

 Various carriers that are pharmaceutically acceptable can be added to the antitumor agent of the present invention. For example, it may contain excipients, binders, disintegrants, lubricants, flavoring agents, coloring agents, sweeteners, corrigents, solubilizers, suspending agents, emulsifiers, coating agents. However, it is not limited to these. It is good also as a long-lasting and sustained-release thing for the Hanabira bamboo solvent extract composition of this invention. The cosmetics of the present invention are cosmetics, quasi drugs, aqueous components used in pharmaceuticals, oily components, plant extracts, animal extracts, powders, surfactants, oils, alcohols, pH adjusters, preservatives, It is adjusted by appropriately blending antioxidants, thickeners, pigments, fragrances and the like as necessary. Furthermore, the cosmetic of the present invention may contain other active ingredients such as a whitening agent, a moisturizing agent, an anti-inflammatory agent, and an ultraviolet absorber in addition to the above.

The health food of the present invention is prepared in the form of moss, bread, candy, jelly, cookie, soup by blending the extract of the solvent extract of the present invention into the raw material of food and drink so as to have the above blending amount. It can be. In addition, inorganic components such as iron and calcium, various vitamins, dietary fibers such as oligosaccharides and chitosan, proteins such as soybean extract, Lipids such as tin and sugars such as sucrose and lactose can also be added.

 The subject of the present invention is not particularly limited as long as the effect is exhibited, but is preferably a warm-blooded animal, more preferably a mammal, and most preferably a human.

 Example

 [0024] Hereinafter, the present invention will be described in detail by way of examples, but these examples do not limit the present invention in any way.

[0025] Production Example 1 [Production of Hanabiratake fruit bodies]

 Hanabira bamboo fruit bodies were produced by the following method.

 Contains larch sawdust, flour, nutrients (banana, honey, ebios, peptone, calcium chloride, hyponex) and water, sawdust: flour: nutrients: water = 100: 11. 5: 1. 9: 51 by weight A fungus bed substrate was prepared. The fungus bed base material (520 g) was placed in an 850 cc polypropylene culture bottle, and the culture bottle was sterilized according to a conventional method, followed by inoculation with an inoculum of Hanabiratake (16 g). Thereafter, the fruit bodies of the bamboo leaf were harvested by culturing the culture bottle at a temperature of 23 ° C. for 56 days. The weight of the fruiting body was 140 g per culture bottle

[0026] Production Example 2 [Production of Hanabiratake Mycelium]

 Hanabiratake mycelium was produced by the following method.

 Yeast extract 0.4%, glucose 2%, potassium dihydrogen phosphate 0.1%, hydrogen phosphate 2 sodium 0.1% 200 mL of the prepared medium was placed in a 5 OOmL Erlenmeyer flask and sterilized according to a conventional method. Agar plates with a diameter of 6 mm were punched out and inoculated into this liquid medium. Hanabiratake mycelium was harvested by shaking culture in the dark at 24 ° C for 21 days. The dry weight of the mycelium was 2 g per Erlenmeyer flask.

[0027] Example 1 [Preparation of small body anti-tumor component of Hanabiratake fruit body]

The dried fruit of Nabinotake mushroom fruit obtained in Production Example 1 was made into a powder with a blender, and 2 g of water was added to 50 g of the dried product, followed by extraction at 100 ° C. for 2 hours. The supernatant was collected by centrifugation, and the same extraction operation was repeated twice more on the precipitate. Recovered The supernatant was freeze-dried to obtain 23 g of extract (Hanabiratake fruiting body hot water extract; SCFHEx). The extract was again dissolved in 500 mL of water, and dialyzed with a dialysis membrane having a molecular weight of 8000 cut (Funakoshi, Spectranotech membrane pore 1.1). The dialyzed external solution was collected and freeze-dried to obtain 2.3 g (referred to as SCFHL) of a small-sized anti-tumor component of the fruit body of Hanabiratake.

 [0028] Example 2 [Preparation of low molecular weight antitumor component of mycelium mycelia]

 The dry matter of the mycelium of Nabinotake mushroom obtained in Production Example 2 was made into a powder by using a blender, and 200 mL of water was added to 5 g of the dried powder, followed by extraction at 100 ° C. for 1 hour. The supernatant collected by centrifugation was freeze-dried to obtain 1.6 g of extract (Hanabiratake mycelium hot water extract; SCMHEx). The extract was again dissolved in 200 mL of water and dialyzed with a dialysis membrane having a molecular weight of 8000 cut (Funakoshi, Spectra Biotech Membrane Pore 1.1). The dialyzed external solution was collected and freeze-dried to obtain 0.62 g (referred to as SCMHL) of Hanabiratake mycelium low molecular weight antitumor component.

 [0029] Example 3 [Preparation of small body antitumor component of Hanabiratake fruit body 2]

 The dried fruit of Nabinotake mushroom fruit obtained in Production Example 1 was made into a powder with a blender, and 2 g of water was added to 50 g of the dried product, followed by extraction at 100 ° C. for 2 hours. The supernatant was collected by centrifugation, and the same extraction operation was repeated twice more on the precipitate. The collected supernatant is freeze-dried, and lg of the resulting extract is dissolved in 50 mL of water, and the flow rate is 200 mmZ on a column (φ 30 mm X 900 mm, packing amount 500 mL) packed with Sephadex G-75 gel filtration carrier. It was applied at the same time and subsequently developed with distilled water. 50 mL fraction fraction GPC method (column; UltrahydrogelGuard + 120 + 500; Waters, mobile phase; 0.5 M phosphate buffer (pHl l), flow rate; 0.5 mLZ, detection; differential refractive index The estimated molecular weight is calculated from the retention time of dextran of each molecular weight), and the molecular weights of 500 to 10,000 are collected and freeze-dried. Obtained.

 [0030] Comparative Example 1 [Preparation of small-sized anti-tumor component of Agarita statake fruiting body]

The dried fruit body of Agarita statake was powdered with a blender, and 2 L of water was added to 36 g of the powder, and extraction was performed at 100 ° C for 2 hours. The supernatant is recovered by centrifugation and turned into a precipitate. The same extraction operation was repeated twice more. The collected supernatant was lyophilized to obtain 25 g of extract. The extract was dissolved again in 500 mL of water and dialyzed with a dialysis membrane having a molecular weight of 8000 cut (Funakoshi, Spectra Biotech Membrane Pore 1.1). The dialyzed external solution was collected and freeze-dried to obtain 10.9 g (ABF HL).

 [0031] Test Example I [quantitative determination of j8 dulcan]

 The amount of j8 glucan was quantified by the Congo-Red method with respect to the extract of Shiratake mushroom hot water (SCFHEx, SCMHEx) and the low molecular weight antitumor component (SCFHL, SCMHL) prepared according to Examples 1 and 2. Each 2% aqueous solution was prepared and β-glucan was quantified using Congo Red reagent. SCFEx was 5 mgZmL (25% solids), SCMEx was lmgZmL (5% solids) β-glucan. The low molecular weight component obtained by fractionation, SCFHL, is 0.08 mg / mL (0.4% solids) or less, and SCMHL is 0.03 mg / mL (0. Only the following j8 glucan was detected.

 [0032] The j8 glucan quantified by the Congo-Red method is said to have a molecular weight of 100,000 or more.

 [0033] From this, 96% or more of β-glucan (molecular weight tens of thousands to hundreds of thousands) contained in SCFEx and SCMEx was removed by fractionation treatment with a dialysis membrane. It became clear that it was not included. Also, Congo Red's metachroma (change in color tone, shift in absorption maximum) (see JP 2000-217543 A) was not observed in SCFHL or SCM HL.

 [0034] Test Example 2 [Confirmation of molecular weight distribution 1]

 When the molecular weight distributions of Nobuna bamboo fruit body hot water extract (SCFHEx) and Hanabira bamboo fruit body anti-tumor component (SCFHL) prepared according to Example 1 were confirmed by the GPC method (above), the main peak of the hot water extract was confirmed. Showed a molecular weight of around 50,000, while the low molecular weight antitumor component was around 5,000. The chart is shown in Fig. 1.

 [0035] Test Example 3 [Confirmation of molecular weight distribution 2]

Noburi Natake Mycelium Hot Water Extract (SCMHEx), Hanabila, Prepared according to Example 2 The molecular weight distribution of the bamboo mycelium low molecular weight antitumor component (SCMHL) was confirmed by the GPC method (described above). The main peak of the hot water extract showed a molecular weight of around 50,000, while the low molecular weight antitumor component was low. The tumor component was around 5000.

 [0036] Test Example 4 [cancer cell growth inhibition test]

 Nobunatake bamboo fruit body hot water extract (SCFHEx), Hanabiratake mycelium hot water extract (SCMHEx), Hanabiratake fruit body small molecule antitumor component (SCFHL), Hanabiratake mycelium small molecule antitumor component prepared in Example 2 ( SCMHL) and Agaric statake fruit body small molecule antitumor component (ABFHL) prepared in Comparative Example 1 were dissolved in phosphate buffer (PBS, pH 7.2) and adjusted to a concentration of lOmgZmL. Human colon cancer cells (Caco-2) or human leukemia cells (HL-60) are suspended in RPMI1640-10% urine serum medium (pH 7.2) to a concentration of 60,000 ZmL. 90 L was dispensed into 96-well plates. Dispense 10 μL each of the above samples (10 μL of PBS alone into the blank and 10 μL of actinomycin D adjusted to a concentration of 1 μgZmL for the positive control). After mixing, incubate for 48 to 72 hours at 37 ° C in a 5% carbon dioxide atmosphere, and count the number of living cells with Cell Counting Kit 8 (manufactured by Dojin University) and compare it with the amount of cell growth in the blank. The cell growth inhibition rate was calculated.

 [0037] The results are shown in Table 1.

 [0038] [Table 1]

表 1の結果カゝら明らかなように、本発明のハナビラタケ低分子抗腫瘍成分は、がん 細胞の増殖を抑制する効果を示し、その強度は単なる熱水抽出物と比べると有意に 高くなつており、透析膜による分画が、がん細胞の増殖抑制効果を発揮するのに有 効であることが明らかになった。

As can be seen from the results in Table 1, the low molecular weight anti-tumor component of the present invention exhibits an effect of suppressing the growth of cancer cells, and its strength is significantly higher than that of a mere hot water extract. The fractionation by dialysis membrane is effective for exerting the growth inhibitory effect of cancer cells. It became clear that it was effective.

 [0040] Test Example 5 [Tumor Growth Inhibition Test by Ingestion]

 The mouse (ICR: Jcl, 6 weeks old, female, 10 animals in group Z) was suspended in 腋 · MEM serum-free medium (pH 7.2) at a concentration of 40000000 / mL in the lower right limb. Mouse sarcoma cells (SI 80) were transplanted at 50 μL each. Hanabiratake fruit body small molecule antitumor component (SCFHL), Hanabiratake mycelium small molecule antitumor component (SCMHL), Agarita Takeko prepared according to Examples 1 and 2 and Comparative Example 1 from the 7th day from the date of transplantation Substantial small molecule anti-tumor component (ABFHL) and an aqueous solution (concentration: 18 mgZmL) of an extract of hot water bamboo (SCFHEx, SCMHEx) were orally administered at 50 μL each (50 L tap water in the blank). 30 mg / kg body weight). The tumor size was measured every 3 or 4 days for 35 days from the date of transplantation, and the change with time was observed. The tumor mass was excised on the final day, and the tumor growth inhibition rate was calculated from its weight. Tumor size was calculated by the following formula: major axis X minor axis X minor axis ÷ 2.

 [0041] The results are shown in FIG.

[0042] [Table 2]

 [Results of tumor growth inhibition test by oral intake of extract]

[0043] As is clear from the results of Fig. 2 and Table 2, the low-molecular weight anti-tumor component of the present invention shows an effect of suppressing the growth of cancer cells, and its strength is higher than that of a mere hot water extract. Each increase was about 10%, and it became clear that fractionation with a dialysis membrane was effective in exerting an inhibitory effect on the growth of cancer cells.

Although the invention has been described in detail and with reference to specific embodiments, it will be apparent to those skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the invention. This application is based on a Japanese patent application filed on May 25, 2005 (Japanese Patent Application No. 2005-152454), the contents of which are incorporated herein by reference.

Industrial applicability

 The present invention provides an extract of Hanabiratake, whose main component is a relatively low molecular weight component obtained from the fruit body or mycelia of Hanabiratake, and an antitumor agent, cosmetic and health food containing the same as an active ingredient.

Claims

The scope of the claims  [I] Hanabiratake solvent extract composition whose main component is a molecular weight of 500 to: LOOOO.  [2] A composition of the extract of Hanabira bamboo obtained by fractionating components having a molecular weight in the range of 500 to 10,000 from a solvent extract of Hanabiratake using a dialysis membrane. [3] A composition of the extract of Hanabira bamboo obtained by fractionating components having a molecular weight in the range of 500 to 10,000 from a solvent extract of Hanabiratake by gel filtration. [4] The main component of the molecular weight is 50 0 to: An extract of Hanabira bamboo obtained by fractionating components in the range of L0000. [5] The component having a molecular weight in the range of 500 to 10000 is contained in the dry weight of the extract of Hanabiratake. The extract of Hanabiratake according to any one of claims 1 to 4, which is 80% by weight to 100% by weight.  [6] The garlic bamboo extract composition according to any one of [1] to [4], wherein the content of j8 dulcan is 5% by weight or less in the dry weight of the garlic bamboo extract composition. [7] The Hanabiratake extract composition according to any one of claims 1 to 4, wherein the solvent is hot water. [8] An antitumor composition comprising the composition of the extract of any one of claims 1 to 7 as an active ingredient.  [9] An antitumor agent comprising as an active ingredient the Hanabiratake extract composition according to any one of claims 1 to 7.  [10] A cosmetic composition comprising as an active ingredient the Hanabiratake extract composition according to any one of claims 1 to 7.  [II] A health food composition comprising as an active ingredient the Hanabiratake extract composition according to any one of claims 1 to 7.