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WO2006121222A1 - Dispositifs destines a contenir des rongeurs et des vecteurs lors d'experiences de transmission parasitaire en laboratoire - Google Patents

Dispositifs destines a contenir des rongeurs et des vecteurs lors d'experiences de transmission parasitaire en laboratoire Download PDF

Info

Publication number
WO2006121222A1
WO2006121222A1 PCT/KE2006/000014 KE2006000014W WO2006121222A1 WO 2006121222 A1 WO2006121222 A1 WO 2006121222A1 KE 2006000014 W KE2006000014 W KE 2006000014W WO 2006121222 A1 WO2006121222 A1 WO 2006121222A1
Authority
WO
WIPO (PCT)
Prior art keywords
rodent
vector
cage
holding compartment
holding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KE2006/000014
Other languages
English (en)
Inventor
Ndungu Kariuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KARI- TRYPANOSOMIASIS RESEARCH CENTRE
Original Assignee
KARI- TRYPANOSOMIASIS RESEARCH CENTRE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KARI- TRYPANOSOMIASIS RESEARCH CENTRE filed Critical KARI- TRYPANOSOMIASIS RESEARCH CENTRE
Publication of WO2006121222A1 publication Critical patent/WO2006121222A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K1/00—Housing animals; Equipment therefor
    • A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
    • A01K1/03—Housing for domestic or laboratory animals
    • A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/30—Rearing or breeding invertebrates

Definitions

  • 2003/0051668 Al disclosed a milking apparatus for laboratory animals such as rats by a single experimenter It is designed to only hold the rodent and has no provision for the vector making it therefore not amenable for use du ⁇ ng transmission expe ⁇ ments Patrick R.Hughes, Ithaca, in US patent No. 5,351,643 disclosed an insect rearing device not amenable for use during transmission experiments since it is designed to only hold insects when rearing CharlesW. Carr, Edgewater and Douglas M. Kolodny- Hirsch, Fells point, in US patent No. 5,178,084 discloses an apparatus for mass rearing of Entomoparasites and production of insect related Entomopathogens and therefore not amenable in vector transmission studies Thomas N.
  • transmission used in this application refers to picking of the infection from an infected host by the vector and transferring it to an uninfected host
  • vector refers to any insect capable of picking and transferring the infection e g mosquito, tsetse fly, sandfly and other biting flies
  • the advantages of the disclosed devices are i) Vector infection and transmission can take place with minimal handling by the investigator ii) The chances of investigator being bitten by either the vector or rodent are minimized iii) In order to avoid isolation-related stress during the experiments, the units can be assembled in a manner that allow the rodents to see each other iv) These devices are portable and can easily fit in an incubator and therefore it is possible to do transmission studies in the field as well as under controlled environment i e at optimum temperature, light and humidity v) Vectors can be left to feed at their own pace while the investigator carries out other work in the laboratory vi) The devices can be modified to handle rodents of various sizes for various transmission expe ⁇ ments vii) The devices can be used while infecting rodents intravenously (IV) and bleeding rodents from the tail in the laboratory viii) The device may be modified to enable mating and hatching of vectors in a controlled environment (incubator) xiv) Reduction in personnel involved when carrying out transmission experiments
  • Transmission process comprises infecting the vector en mass on an infected rodent followed by each infected vector singly transmitting the infection to un infected rodent
  • Mass vector infection method comprise the following steps:
  • Single vector infection transmission method comprise the following steps:
  • Rodent and vector holder (Fig 1) during the process of single vector transmitting infection to the rodent This is made up of the following parts: - a) Rodent holding compartment (1,2,3,4,5,6) b) Single vector cage (Fig 5) c) Vector cage holder (12 &13) d) Netting material (Fig 5: 71) (v) Special cages for introducing the rodent into rodent holding compartment
  • the infected vector contacts the uninfected rodent through (11) and transmits infection.
  • the single vector cage (Fig: 5) is slotted first before introducing the rodent so as to seal this opening.
  • Rodent resting base (7) made of metal plate or other suitable material is fixed on the lower side of rodent holding compartment.
  • Vector tube receptacle (13) attached to the rodent holding compartment by (12) holds the vector tube upright while fixed on (74).
  • the stand (14) with a resting base (IS) is fixed onto the lower side of the rodent holding compartment to provide means for resting it on the bench.
  • the rodent is released through door (18) with a handle (19) and walk through the ladder (A) to the rodent-resting cage (Fig. 6).
  • the netting material is held on to the tube by adhesive tape (72) or any other appropriate means of attaching.
  • the piston (73) serves as a stopper and is used to restrain the movement of the vector within the tube by reducing the area available for its free movement.
  • This skeleton is wrapped with an expanded wire or any other appropriate material (8) leaving three open spaces one on the upper side (11) of the cage that allows the vector inside the vector cage (Fig: 4) direct contact with the rodent.
  • the vector cage is slotted before the rodent is introduced into the cage.
  • the rodent (mouse) is placed in the special rodent introducing cage (Fig 3) and directed into the rodent holding compartment through (16) as in Fig 1 and prevented from escaping by a door (9) with a notch (10) that enables hanging of the tail outside the cage.
  • Rodent release door This is opened at the end of experiments and allows the rodent access to the ladder (A) through which it walks to its resting cage ( Figure 6). It can be made of piano wire or any appropriate material (g) Ladder (A)
  • Several such units can be joined together in any configuration to allow handling of multiple vectors and rodents.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Housing For Livestock And Birds (AREA)
  • Catching Or Destruction (AREA)

Abstract

L'invention concerne un dispositif destiné à contenir un rongeur et un vecteur pendant un processus d'infection et de transmission de vecteur (figures 7, 8). Ce dispositif comprend un compartiment destiné à contenir un rongeur (figures 1, 2 - 1, 2, 3, 4, 5, 6) et une cage de vecteur (figure 4). Le compartiment destiné à contenir un rongeur est pourvu d'un support destiné à reposer sur la table de manipulation (figures 1, 2 14, 15) et d'un moyen destiné à venir en contact avec la cage du vecteur (figures 1; 12, 13), et il est doté d'une porte coulissante de libération de rongeur (figures 1; 18) et d'une échelle (figures 1, 2-A). L'échelle mène le rongeur à une cage de repos (figure 6) à travers une entrée (75) fermée par une porte (74) et le compartiment destiné à contenir le rongeur s'accompagne d'une cage spéciale pour rongeur (figure 3) permettant d'introduire le rongeur dans le compartiment. Cette cage spéciale comprend un élément (A) relié à une porte (B) pour former une enceinte, et une voie de sortie de rongeur (C).
PCT/KE2006/000014 2005-05-11 2006-05-11 Dispositifs destines a contenir des rongeurs et des vecteurs lors d'experiences de transmission parasitaire en laboratoire Ceased WO2006121222A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KE40905 2005-05-11
KEKE/P/04/00409 2005-05-11

Publications (1)

Publication Number Publication Date
WO2006121222A1 true WO2006121222A1 (fr) 2006-11-16

Family

ID=37396704

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KE2006/000014 Ceased WO2006121222A1 (fr) 2005-05-11 2006-05-11 Dispositifs destines a contenir des rongeurs et des vecteurs lors d'experiences de transmission parasitaire en laboratoire

Country Status (1)

Country Link
WO (1) WO2006121222A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010144494A3 (fr) * 2009-06-08 2011-12-01 Purdue Research Foundation Système d'automatisation de protocoles de test sur animaux
CN113743213A (zh) * 2021-08-02 2021-12-03 日立楼宇技术（广州）有限公司 一种辅助特殊乘客乘梯的方法、装置及存储介质

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3028839A (en) * 1960-10-14 1962-04-10 James W Crook Animal restrainer and exposure chamber
US4941431A (en) * 1988-12-14 1990-07-17 Anderson Francis G System for handling laboratory animals
US5074247A (en) * 1991-02-11 1991-12-24 The United States Of America As Represented By The Secretary Of The Army Insect containing test apparatus
US5099792A (en) * 1991-04-08 1992-03-31 Battelle Memorial Institute Interchangeable whole-body and nose-only exposure system
US5930944A (en) * 1997-02-18 1999-08-03 Kness Mfg. Co., Inc. Apparatus and method for trapping and monitoring mice and insects
GB2340822A (en) * 1998-08-21 2000-03-01 Univ Bristol A container for parasites

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3028839A (en) * 1960-10-14 1962-04-10 James W Crook Animal restrainer and exposure chamber
US4941431A (en) * 1988-12-14 1990-07-17 Anderson Francis G System for handling laboratory animals
US5074247A (en) * 1991-02-11 1991-12-24 The United States Of America As Represented By The Secretary Of The Army Insect containing test apparatus
US5099792A (en) * 1991-04-08 1992-03-31 Battelle Memorial Institute Interchangeable whole-body and nose-only exposure system
US5930944A (en) * 1997-02-18 1999-08-03 Kness Mfg. Co., Inc. Apparatus and method for trapping and monitoring mice and insects
GB2340822A (en) * 1998-08-21 2000-03-01 Univ Bristol A container for parasites

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010144494A3 (fr) * 2009-06-08 2011-12-01 Purdue Research Foundation Système d'automatisation de protocoles de test sur animaux
US9497928B2 (en) 2009-06-08 2016-11-22 Purdue Research Foundation System for automating animal testing protocols
CN113743213A (zh) * 2021-08-02 2021-12-03 日立楼宇技术（广州）有限公司 一种辅助特殊乘客乘梯的方法、装置及存储介质
CN113743213B (zh) * 2021-08-02 2023-11-24 日立楼宇技术（广州）有限公司 一种辅助特殊乘客乘梯的方法、装置及存储介质

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