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WO2006113198A3 - Procedes permettant de faciliter l'isolation d'arn dans des echantillons biologiques - Google Patents

Procedes permettant de faciliter l'isolation d'arn dans des echantillons biologiques Download PDF

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Publication number
WO2006113198A3
WO2006113198A3 PCT/US2006/013251 US2006013251W WO2006113198A3 WO 2006113198 A3 WO2006113198 A3 WO 2006113198A3 US 2006013251 W US2006013251 W US 2006013251W WO 2006113198 A3 WO2006113198 A3 WO 2006113198A3
Authority
WO
WIPO (PCT)
Prior art keywords
rna
solid phase
methods
biological samples
enhancing
Prior art date
Application number
PCT/US2006/013251
Other languages
English (en)
Other versions
WO2006113198A2 (fr
Inventor
Hashem Akhavan-Tafti
Original Assignee
Nexgen Diagnostics Llc
Hashem Akhavan-Tafti
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nexgen Diagnostics Llc, Hashem Akhavan-Tafti filed Critical Nexgen Diagnostics Llc
Priority to AU2006236894A priority Critical patent/AU2006236894A1/en
Priority to CA002604631A priority patent/CA2604631A1/fr
Priority to JP2008507707A priority patent/JP2008536513A/ja
Priority to EP06740785A priority patent/EP1872131A4/fr
Publication of WO2006113198A2 publication Critical patent/WO2006113198A2/fr
Publication of WO2006113198A3 publication Critical patent/WO2006113198A3/fr
Priority to IL186689A priority patent/IL186689A0/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention concerne un procédé permettant de faciliter l'isolation d'acides ribonucléiques dans des échantillons de matériau biologique ou cellulaire. Ledit procédé consiste à utiliser une solution d'un agent pendant la liaison d'acides nucléiques à un matériau de liaison en phase solide qui, après élution, favorise la récupération d'ARN. Le nettoyage de la phase solide et l'élution de l'acide nucléique permettent de produire un ARN ayant un rendement et/ou une pureté améliorés. Ce nouveau procédé permet de capturer et de libérer l'ARN sous une forme convenant au traitement en aval en mois de cinq minutes. Les agents préférés selon l'invention renferment des composés organiques monomères, oligomères, dendrimères et polymères possédant des charges positives multiples.
PCT/US2006/013251 2005-04-19 2006-04-07 Procedes permettant de faciliter l'isolation d'arn dans des echantillons biologiques WO2006113198A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2006236894A AU2006236894A1 (en) 2005-04-19 2006-04-07 Methods of enhancing isolation of RNA from biological samples
CA002604631A CA2604631A1 (fr) 2005-04-19 2006-04-07 Procedes permettant de faciliter l'isolation d'arn dans des echantillons biologiques
JP2008507707A JP2008536513A (ja) 2005-04-19 2006-04-07 生体試料からのｒｎａの促進された単離方法
EP06740785A EP1872131A4 (fr) 2005-04-19 2006-04-07 Procedes permettant de faciliter l'isolation d'arn dans des echantillons biologiques
IL186689A IL186689A0 (en) 2005-04-19 2007-10-16 Methods of enhancing isolation of rna from biological samples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/109,366 2005-04-19
US11/109,366 US20060234251A1 (en) 2005-04-19 2005-04-19 Methods of enhancing isolation of RNA from biological samples

Publications (2)

Publication Number Publication Date
WO2006113198A2 WO2006113198A2 (fr) 2006-10-26
WO2006113198A3 true WO2006113198A3 (fr) 2007-09-07

Family

ID=37108936

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/013251 WO2006113198A2 (fr) 2005-04-19 2006-04-07 Procedes permettant de faciliter l'isolation d'arn dans des echantillons biologiques

Country Status (9)

Country Link
US (1) US20060234251A1 (fr)
EP (1) EP1872131A4 (fr)
JP (1) JP2008536513A (fr)
KR (1) KR20080005213A (fr)
CN (1) CN101203758A (fr)
AU (1) AU2006236894A1 (fr)
CA (1) CA2604631A1 (fr)
IL (1) IL186689A0 (fr)
WO (1) WO2006113198A2 (fr)

Cited By (10)

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USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing

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US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
EP2407243B1 (fr) 2003-07-31 2020-04-22 Handylab, Inc. Dispositif multicouche microfluidique
US20050106602A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Simplified methods for isolating nucleic acids from cellular materials
US20050136477A1 (en) * 2003-11-17 2005-06-23 Hashem Akhavan-Tafti Methods for isolating nucleic acids from biological and cellular materials
CA2994321C (fr) * 2004-05-03 2023-08-08 Handylab, Inc. Un dispositif microfluidique et ses methodes pour le traitement d'echantillons contenant des polynucleotides
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
WO2007094506A1 (fr) * 2006-02-15 2007-08-23 Tosoh Corporation Procédé d'extraction d'acide nucléique à partir d'un matériau biologique
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
WO2007112114A2 (fr) 2006-03-24 2007-10-04 Handylab, Inc. Système intégré de traitement d'échantillons microfluides et son procédé d'utilisation
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2008061165A2 (fr) 2006-11-14 2008-05-22 Handylab, Inc. Cartouche microfluidique et son procédé de fabrication
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
DE102008010693A1 (de) * 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrixmaterialien, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
JP2013513400A (ja) * 2009-12-14 2013-04-22 ベティ ウー 核酸物質を分離する方法および物質
CN106190806B (zh) 2011-04-15 2018-11-06 贝克顿·迪金森公司 扫描实时微流体热循环仪和用于同步的热循环和扫描光学检测的方法
WO2013067202A1 (fr) 2011-11-04 2013-05-10 Handylab, Inc. Dispositif de préparation d'échantillons de polynucléotides
EP2810080B1 (fr) 2012-02-03 2024-03-27 Becton, Dickinson and Company Fichiers externes servant à répartir des tests de diagnostic moléculaire et à déterminer la compatibilité entre les tests
WO2014176435A2 (fr) 2013-04-25 2014-10-30 Bergo Vladislav B Compositions de microréseaux et leurs procédés d'utilisation
US20160010081A1 (en) * 2014-07-11 2016-01-14 Exact Sciences Corporation Systems, methods and kits for extracting nucleic acid from digestive fluid
FR3031063B1 (fr) * 2014-12-30 2017-02-10 Biomerieux Sa Complexe multicouches, procede de fabrication et utilisation du complexe
JP7142569B2 (ja) * 2015-09-09 2022-09-27 ドローブリッジ ヘルス，インコーポレイテッド サンプルの収集、安定化、および保存のためのシステム、方法およびデバイス
GB2590813B (en) 2017-01-10 2021-10-27 Drawbridge Health Inc Devices, systems, and methods for sample collection
KR20200051699A (ko) 2017-09-07 2020-05-13 아뎁트릭스 코퍼레이션 프로테오믹스를 위한 멀티플렉스화된 비드 어레이
KR102136695B1 (ko) * 2018-08-22 2020-07-22 주식회사 인퓨전텍 현장 진단용 장치를 이용한 병원체 농축 및 핵산 추출 방법
KR102117833B1 (ko) * 2019-01-04 2020-06-02 남장희 생물시료로부터 유전물질을 신속하게 분리하는 핵산 흡착 물질을 포함하는 조성물 및 그를 이용하여 생물시료로부터 유전물질을 신속하게 분리하는 방법
TWI830144B (zh) * 2021-10-19 2024-01-21 英華達股份有限公司 篩選裝置及磁力架

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US11845081B2 (en) 2007-07-13 2023-12-19 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
US9480983B2 (en) 2011-09-30 2016-11-01 Becton, Dickinson And Company Unitized reagent strip

Also Published As

Publication number Publication date
CN101203758A (zh) 2008-06-18
EP1872131A2 (fr) 2008-01-02
AU2006236894A1 (en) 2006-10-26
JP2008536513A (ja) 2008-09-11
US20060234251A1 (en) 2006-10-19
WO2006113198A2 (fr) 2006-10-26
EP1872131A4 (fr) 2009-04-01
CA2604631A1 (fr) 2006-10-26
IL186689A0 (en) 2008-02-09
KR20080005213A (ko) 2008-01-10

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