WO2006038542A1 - Carotenoid pigment-containing composition having high storage stability, method of producing the same and feed comprising the carotenoid pigment-containing composition - Google Patents

Abstract

It is intended to provide a method of producing a carotenoid pigment-containing composition having a high storage stability; a carotenoid pigment-containing composition having a high storage stability which is obtained by the above method; and a feed which comprises this carotenoid pigment-containing composition having a high storage stability and have an excellent effect of improving the body color or the color hue. Namely, a method of producing a carotenoid pigment-containing composition having a high storage stability characterized by comprising culturing a microorganism capable of producing a carotenoid pigment, adding rice bran as an excipient for elevating the storage stability of the carotenoid pigment to the liquid culture medium or the concentrated liquid culture medium of the microorganism capable of producing a carotenoid pigment thus obtained, and then drying; a carotenoid pigment-containing composition having a high storage stability which is obtained by this method; and a feed comprising the carotenoid pigment-containing composition having a high storage stability which is obtained by this method.

Description

 Specification

 Carotenoid pigment-containing composition having excellent storage stability, production method thereof, and feed comprising the carotenoid pigment-containing composition

 Technical field

 [0001] The present invention relates to a carotenoid pigment-containing composition excellent in storage stability, used as an agent for improving the body color or color tone of fish and shellfish, or a chicken egg color tone, and a method for producing the same, as well as the carotenoid pigment-containing composition. The present invention relates to a feed comprising the composition as a body color improving or color improving agent.

 Background art

 [0002] Conventionally, to improve body color such as Thai, shrimp, and power, improve body color such as salmon and trout, or improve egg yolk color tone, improve body color or color tone in feed Ingredients are being formulated.

 As a body color improver or color improver, natural pigments derived from plants such as paprika and marigold, and pigments derived from microorganisms such as spirilna, hematococcus, fafia, paracoccus, and synthetic compounds, istaxanthin, canthaxanthin, β apo 8 ' —Rikiguchi Ethyl cinnamate is known.

 [0003] Generally, these carotenoid pigments are quite unstable, and the amount of carotenoid pigments contained decreases with the passage of storage period under the high temperature and high humidity conditions that are the general environment for feed storage. Is known.

 [0004] In order to suppress it, for example, it is known to mix synthetic additives such as ethoxyquin, butylhydroxyl toluene (ブ), butylhydroxy diazole (ΒΗΑ), but in recent years food safety In some cases, these additives may be required to be natural products.

 [0005] As natural products, flavonoids such as rutin, sunflower extract, plant extracts such as phytic acid, tocopherol, ascorbic acid, quenate and the like may be mixed as additives.

Of these stabilizers, vitamins, flavonoids, and plant extracts are generally expensive and practically difficult to use as stabilizers for carotenoid pigments. Also Asco Rubinic acid and citrate have problems when used as stabilizers for carotenoid pigments that are less effective (see, for example, Patent Document 1).

[0006] On the other hand, focusing on the fact that decomposition of these carotenoid pigments is due to oxygen decomposition in the air, a method of sealing with an oxygen-impermeable packaging material together with an oxygen scavenger has been developed. (For example, see Patent Document 2) However, the stability after opening or mixing with feed is not sufficient, and further improvement is necessary.

[0007] Patent Document 1: Japanese Patent Application Laid-Open No. 6-264055 (conventional technology column)

 Patent Document 2: JP-A-8-12896

 Disclosure of the invention

 Problems to be solved by the invention

[0008] Firstly, the present invention eliminates the problems of the prior art, cultivates microorganisms that produce carotenoid pigments, and mixes rice bran, which is a relatively inexpensive natural product, with the obtained culture solution. Accordingly, an object of the present invention is to provide a method for producing a carotenoid pigment-containing composition having excellent storage stability under high temperature and high humidity conditions, which is a general environment for feed storage.

 Secondly, an object of the present invention is to provide a carotenoid pigment-containing composition that is produced by such a method and has excellent storage stability.

 Thirdly, the present invention provides a feed excellent in body color improvement or color tone improving ability, which is prepared by such a method and is formulated with a carotenoid pigment-containing composition having excellent storage stability. It is intended.

 Means for solving the problem

[0009] As a result of intensive studies to solve the above problems, the present inventors unexpectedly cultured microorganisms that produce carotenoid pigments, and used an excipient for drying the resulting culture solution. The use of fat-soluble anti-acid products such as rice bran containing significant amounts of phytic acid, ferulic acid, γ-oryzanol, and tocols significantly reduces the degradation of carotenoid pigments at high temperatures and high humidity. It was found that it could be stable for a long time even under conditions.

That is, the present inventors include a carotenoid pigment-containing culture solution used as an agent for improving the body color of seafood or the color tone of chicken eggs, by drying using rice bran as an excipient. It was found that the decomposition of the carotenoid pigment contained therein was suppressed, and that it could be stored stably for a long period of time even under high temperature and high humidity conditions, and the present invention was completed based on this finding.

 That is, the present invention according to claim 1 is characterized in that a microorganism producing a carotenoid pigment is cultured, rice bran is mixed with the obtained culture solution or culture concentrate of the microorganism producing the carotenoid pigment, and then dried. The present invention provides a method for producing a carotenoid pigment-containing composition having excellent storage stability.

 The present invention according to claim 2 provides the method according to claim 1, wherein the microorganism producing a carotenoid pigment belongs to the genus Paracoccus (E ^ acoccus), the genus Haematococcus or the genus Phaffia. To do.

The present invention according to claim 3, Paracoccus _(Par acoccus) genus fine bovine product is a shall provide a method of claim 2 wherein the Paracoccus' force b zeaxanthinifaciens tumefaciens (Paracoccus carotinifaciens).

 The present invention according to claim 4 is a method for producing a carotenoid pigment, wherein the base sequence of the DNA corresponding to 16S ribosome RNA is substantially the same as the base sequence set forth in SEQ ID NO: 1 or the base sequence. A method according to any one of claims 1 to 3 is provided.

 The present invention according to claim 5 is characterized in that the carotenoid pigment producing microorganisms are E-396 strain (FER M BP-4283) and its mutant strain, and A-581-1 strain (FERM BP-4671) and its mutant strain. 5. The method according to claim 4, which is selected by a stock company.

 The present invention according to claim 6 cultivates a microorganism that produces a carotenoid pigment, mixes rice bran with the obtained culture solution or culture concentrate of the microorganism that produces a potent tinoid pigment, and then dries it. The present invention provides a carotenoid pigment-containing composition excellent in storage stability.

 The present invention according to claim 7 is a method for producing a carotenoid pigment, wherein the base sequence of DNA corresponding to 16S ribosome RNA is substantially the same as the base sequence set forth in SEQ ID NO: 1 or the base sequence. A composition according to claim 6 is provided.

The present invention according to claim 8 is characterized in that the carotenoid pigment producing microorganisms are E-396 strain (FER M BP-4283) and its mutant strain, and A-581-1 strain (FERM BP-4671) and its variant. 7. The composition according to claim 6, which is selected from different strains.

 The present invention according to claim 9 provides a feed comprising the composition according to any one of claims 6 to 8.

 The invention's effect

 [0011] First, according to the present invention, a feed storage site is obtained by culturing microorganisms that produce carotenoid pigments and mixing rice bran, which is a relatively inexpensive natural product, with the obtained culture solution or culture concentrate. The present invention provides a method for producing a carotenoid pigment-containing composition that is excellent in storage stability under high-temperature and high-humidity conditions, which are general environments of

 Second, according to the present invention, a carotenoid pigment-containing composition having excellent storage stability is provided.

 Thirdly, according to the present invention, a feed excellent in body color improvement or color tone improvement ability is provided. According to the present invention, the produced carotenoid pigment can be stably stabilized at a low cost by a simple method of using rice bran as an excipient for drying a culture solution or a culture concentrate. That is, rice bran is mixed as an excipient for improving the storage stability of carotenoid pigments into a culture solution or culture concentrate of microorganisms that produce carotenoid pigments, and then dried to obtain carotenoid pigments produced by microorganisms. It is possible to stabilize effectively.

 BEST MODE FOR CARRYING OUT THE INVENTION

 Hereinafter, the present invention will be described in detail.

 The present invention according to claim 1 relates to a method for producing a carotenoid pigment-containing composition having excellent storage stability, culturing a microorganism that produces a carotenoid pigment, and a culture solution or culture of the microorganism that produces the resulting carotenoid pigment. It is characterized by mixing rice bran with the concentrate and then drying.

[0013] The carotenoid pigment in the present invention is not particularly limited. Examples include cryptoxanthin, zeaxanthin, and lycopene. Among these, those having a hydroxyl group may exist as a monolith or in the form of a fatty acid ester. The microorganism producing the carotenoid pigment used in the present invention is not particularly limited as long as it produces one or more of these carotenoid pigments, and examples thereof include bacteria, algae, and yeasts.

 [0014] Specific examples of microorganisms that produce carotenoid pigments include microorganisms belonging to the genus Paracoccus, the genus Haematococcus or the genus Phaff.

 Examples of the Paracoccus genus microorganism include, for example, Paracoccus carotinifaciens, and more specifically, Noracoccus carotinifaciens E as described in claim 3. -396 shares (FE RM BP-4283) and A-581-1 shares (FERM BP-4671). Next, examples of Haematococcus genus cattle include Hematococcus pluvialis.

 Examples of microorganisms belonging to the genus Ehaffia include Phaffia rhodozvma, and more specifically, Phaffia rhodozvma IF 010129 strain, IFO10130 strain, ATCC24201 strain, and the like. .

 [0015] As described in claim 4, the microorganism that produces carotenoid pigment, the base sequence of DNA corresponding to the 16S liposomal RNA is substantially the base sequence of SEQ ID NO: 1 or substantially the same as the base sequence Examples include carotenoid pigment producing microorganisms that are homologous. The term “substantially homologous” as used herein means that the homology is 98% or more in consideration of the error frequency when determining the DNA base sequence.

[0016] Specific examples of the carotenoid pigment-producing microorganism having the above base sequence or a sequence substantially homologous to the base sequence include Paracoccus carotinifaciens E-396 (FERM BP-4283). ) (JP-A-7-79796, JP-A-8-9964, U.S. Pat. No. 5,607,839, U.S. Pat. No. 5,858,761) and the E-396 strain obtained by mutation improvement Mutant strains and Paracoccus carotinifaciens A-581-1 strain (FERM BP-4671) (JP 7-79796, JP 8-9964, U.S. Pat.No. 5,607,839 Patent No. 5,858,761), mutant strains obtained by modifying the A 581-1 strain, and related species I can make it.

 [0017] In the present invention according to claim 1, as a microorganism that produces a carotenoid pigment, specifically, as described in claim 5, Paracoccus carotini § E-396 The strain (FERM BP-4283) and its mutant strains, and Paracoccus carotinifaciens A-581-1 strain (FERM BP-4671) and its mutant strains are also preferred.

 The above Paracoccus. Carochi-Facience E-396 strain was established on April 27, 1993, at 1-chome Tsukuba 1-chome, Ibaraki, Japan. The deposit number is FERM BP-4283. In addition, Paracoccus' Carochi-Facience A-581-1 share was established in 1-6, Ibaraki Pref., Ibaraki Pref., 1-chome, 1 1 Central Central 6th National Institute of Advanced Industrial Science and Technology, Patent Biology Depositary Center Deposited on the 20th of the month, the deposit number is FERM BP-4671.

 [0018] The DNA base sequence of SEQ ID NO: 1 corresponds to the ribosomal RNA of Paracoccus 'caroti-faciens E-396 strain, and the DNA base sequence of SEQ ID NO: 2 is Noracoccus' caroti-faciens A- It corresponds to 581-1 strain of ribosomal RNA. The base sequence homology of the ribosomal RNAs of E-396 and A- 581-1 strains is 99.4%, which are very closely related strains. These strains are a group of microorganisms that produce carotenoid pigments. To form.

 In the present invention according to claim 1, the carotenoid pigment is added to the culture solution or culture concentrate of the microorganism that produces the carotenoid pigment obtained by culturing the above-described microorganism that produces the carotenoid pigment. Rice bran is mixed as an excipient to improve the storage stability of rice, and then dried.

 [0020] As a method for culturing a microorganism that produces a carotenoid pigment, any method may be used as long as it is a condition for producing a carotenoid pigment. For example, the following method can be employed.

That is, a medium containing a carbon source, a nitrogen source, an inorganic salt, and, if necessary, a special requirement substance (for example, vitamins, amino acids, nucleic acids, etc.) used as a production medium is used. The

 Examples of the carbon source include sugars such as glucose, sucrose, fructose, trehalose, mannose, mannitol and maltose; organic acids such as acetic acid, fumaric acid, citrate, propionic acid, malic acid and malonic acid; Examples include alcohols such as ethanol, propanol, butanol, pentanol, hexanol, and isobutanol.

One or more are used. The addition ratio varies depending on the type of carbon source, but is usually 1 to LOOg, preferably 2 to 50 g per liter of the medium.

 Next, examples of the nitrogen source include potassium nitrate, ammonium nitrate, salt ammonium ammonia, ammonium sulfate, ammonium phosphate, ammonia, urea, and the like. Or two or more are used. The addition ratio varies depending on the type of nitrogen source, but is usually 0.1 to 20 g, preferably 1 to LOg, per 1 L of the medium.

 Examples of inorganic salts include potassium dihydrogen phosphate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, magnesium sulfate, magnesium chloride, iron sulfate, iron chloride, mangan sulfate, salt 匕 manganese, zinc sulfate, Examples include zinc chloride, copper sulfate, calcium chloride, calcium carbonate, sodium carbonate, etc., and one or more of these are used. The amount of added calories varies depending on the type of inorganic salt. Usually, 0.1 mg to LOg per 1 L of medium. In addition, special requirements include vitamins, nucleic acids, yeast extract, peptone, meat extract, malt extract, corn steep liquor, dry yeast, soybean meal, soybean oil, olive oil, corn oil, flax oil 1 type or 2 types or more of these are used. The addition ratio varies depending on the type of special requirement substances, but is usually 0.01 mg to LOOg per 1 L of medium.

 The pH of the medium is adjusted to 2-12, preferably 6-9.

 Culturing is carried out at a temperature of 10 to 70 ° C, preferably 20 to 35 ° C, usually for 1 to 20 days, preferably 2 to 9 days, with shaking or aeration and agitation.

 In the present invention, microorganisms that produce carotenoid pigments are cultured under such conditions.

When cultured in this way, microorganisms that produce carotenoid pigments produce significant amounts of carotenoid pigments inside and outside the cells. [0022] Next, the culture fluid obtained by the above method is usually removed. In order to obtain a carotenoid pigment-containing composition, the degree of water removal required for the culture solution depends on the state of the pigment content and the like of the culture solution.

 In the present invention, rice bran may be mixed with the culture broth itself as an excipient for improving the storage stability of the carotenoid pigment, but generally a filtration operation is first performed. The filtration method can be performed by a normal filtration method, a centrifugal separation method, or the like.

[0023] Next, rice bran is added to the culture solution or culture concentrate obtained by the above method as an excipient for improving the storage stability of the carotenoid pigment and mixed until uniform.

 Rice bran contains fat-soluble antioxidants such as phytic acid, ferulic acid, y oryzanol, and tocols, which suppresses carotenoid pigment degradation and is stable for long periods under high temperature and high humidity conditions. It is suitable for.

[0024] Next, in order to obtain a powder product, the mixture of carotenoid pigment and rice bran obtained by the above method is dried. Examples of the drying method include spray drying, spray granulation drying, drum drying, freeze drying and the like, which are not particularly limited.

 Thus, by the method of the present invention according to claim 1, a target carotenoid pigment-containing composition having excellent storage stability can be produced.

 In addition, the carotenoid pigment-containing composition produced in this manner may be added to a known carotenoid pigment stabilizer, for example, ethoxyquin, butylhydroxyl toluene (BHT), as long as the purpose of the present invention is not impaired as necessary. Butyl hydroxyl-sol (BHA) can be combined.

[0025] In the present invention, a microorganism that produces a carotenoid pigment is cultured, and the resulting culture solution or culture concentrate of the microorganism that produces the carotenoid pigment improves the storage stability of the carotenoid pigment. As an agent, it is necessary to mix rice bran and then dry it. For example, even if rice bran is mixed after drying the culture solution of microorganisms that produce carotenoid pigments, a carotenoid pigment-containing composition with excellent storage stability I can't get anything ヽ

[0026] Next, the present invention according to claim 6 provides the carotenoid pigment-containing composition thus produced. [0027] As described in claim 7, the microorganism that produces carotenoid pigment, the base sequence of DNA corresponding to the 16S liposomal RNA is substantially the base sequence described in SEQ ID NO: 1 or the base sequence. Examples include carotenoid pigment-producing microorganisms that are homologous.

 The term “substantially homologous” as used herein means that the homology is 98% or more in consideration of the error frequency when determining the DNA base sequence.

 Further, examples of the carotenoid pigment-producing microorganism having the above base sequence or a sequence substantially homologous to the base sequence include those described in the explanation relating to claim 4 above.

 [0028] Specifically, the microorganism producing a carotenoid pigment in the present invention according to claim 6, as described in claim 8, Paracoccus caroti nitoM E-396 strain (FERM BP — 4283) and its mutants, and Paracoccus carotinifaciens A— 581—1 (FERM BP-4671) and its mutants are also preferred.

 [0029] Next, the present invention according to claim 9 is a feed comprising the composition according to any one of claims 6 to 8, and the composition according to any one of claims 6 to 8. The product is blended as a body color improving agent or a color tone improving agent.

 Here, as described above, the composition of claim 6 cultivates a microorganism that produces carotenoid pigment, mixes rice bran with the culture solution or culture concentrate of the microorganism that produces carotenoid pigment, Subsequently, a carotenoid pigment-containing composition having excellent storage stability obtained by drying, the composition comprising a carotenoid pigment having excellent storage stability obtained by the method of the present invention according to claim 1. It is a composition. Claims 7 and 8 are subordinate to claim 6 above.

 Accordingly, the production conditions of the carotenoid pigment-containing composition can be carried out in the same manner as in the present invention according to claim 1.

 [0030] As feed raw materials, those that are usually used as feed for cultured fish and shellfish, or for chickens can be used according to the intended use. Examples include fish meat, fish meal, meat and bone meal, soybean oil meal, corn darten meal, wheat flour, vitamins and the like.

The feed of the present invention according to claim 9 is used for such a feed raw material. A carotenoid pigment-containing composition having excellent storage stability obtained by the method is blended as a body color improving agent or a color tone improving agent, and formed into a pellet or mash. The amount of the carotenoid pigment-containing composition excellent in storage stability obtained by the method of the present invention according to claim 1 in the feed is appropriately determined in consideration of the use scene, the period, etc. based on the regulations of the Feed Safety Act, etc. I hope to do it.

 According to the present invention according to claim 9 as described above, a feed excellent in body color improvement or color tone improvement ability is provided.

 Example

 [0031] Hereinafter, the present invention will be further described with reference to examples, but the present invention is not limited thereto.

[0032] Example 1

 This was carried out in accordance with the culture method for E-396 strain (FERM BP-4283) shown in Example 1 of JP-A-2001-352995.

 Specifically, first, lOOmL of a medium having the composition shown in Table 1 was placed in a 500 mL Erlenmeyer flask and steam sterilized at 121 ° C for 20 minutes.

 This was inoculated with 1 platinum ear of a microorganism that produces carotenoid pigment, Paracoccus carotinifaciens (^ me occus carotinifaciens) E-396 strain (FERM BP-4283), and cultured at 28 ° C for 2 days at 220 rpm with shaking. It was.

 10 mL of this culture solution was inoculated into a 2 L Erlenmeyer flask containing 500 mL of the medium having the same composition as described above in Table 1, and subjected to shaking culture at 28 ° C. for 24 hours at 220 rpm.

 Next, 200 mL of this culture broth was inoculated into a 7 L jar fermenter containing 4 L of the medium of the composition shown in Table 2, and the stirring was automatically controlled so that the dissolved oxygen concentration could be maintained at a minimum of 2.5 ppm at 28 ° C, aeration lwm. The culture was carried out for 150 to 170 hours with automatic control using caustic soda so that the pH during the culture could be maintained at 7.2 or higher. Since sucrose is consumed as it grows, 60 g each was added on the first and second days of culture.

The resulting culture broth was centrifuged to concentrate insoluble components, and 48 g and 72 g of rice bran (manufactured by Chinese fats and oils) were added per liter of this concentrate (containing carotenoid pigment 1280 mg / L, dry cell mass 40.4 g / L). Mixed. Spray dryer (inlet air temperature 150 ° C, outlet air temperature 100 The product was dried at ° C to obtain a carotenoid pigment-containing composition powder.

[0033] The obtained carotenoid pigment-containing composition powder was stored in an air atmosphere at 40 ° C and 75% humidity, and the change over time in the carotenoid pigment content during storage for one month was investigated.

 Table 3 shows the ratio of carotenoid pigment remaining after 7, 14, and 30 days to the initial (day 0) content in each experimental group as a ratio (residual rate).

 Table 3 shows that the residual rate of carotenoid pigment increases as the amount of rice bran added increases.

[0034] [Table 1]

[0035] [Table 2]

[0036] [Table 3]

[0037] Example 2 Culture concentrate of Paracoccus carotinifaciens E-396 strain (FERM BP-4283) prepared in the same manner as in Example 1 (containing carotenoid pigment 1 332 mg / L, dry cell mass 41.6 g / L ) 95 g of rice bran (manufactured by Chinese oil and fat) per liter was added and dried with a spray dryer to obtain a carotenoid pigment-containing composition powder.

 100 g of the obtained carotenoid pigment-containing composition powder was mixed with a commercial poultry feed to make 1000 g.

 This was stored in a bag for food preservation under airtight conditions at 40 ° C, humidity 75% and 50 ° C, humidity 75%, and the change over time in the carotenoid pigment content during storage for 1 month was investigated. .

[0038] Table 4 shows the carotenoid pigment residual ratio after 14 and 30 days in each experimental section.

 According to Table 4, when rice bran was not added at 40 ° C and 75% humidity, the residual rate tended to decrease due to mixing with food, whereas in the rice bran added zone, the residual rate increased and maintained. Increased stability was a major factor.

 Even under harsh conditions (50 ° C, humidity 75%), the remaining rate on the 14th day is more than double that of the additive-free zone, about 7 times on the 30th day, and 70% of the first one remains. A long-term stabilization effect under high temperature and high humidity conditions was observed.

[0039] [Table 4]

[0040] Example 3

 The stability of the carotenoid pigment-containing composition powder prepared in Example 2 (rice bran added powder) is generally used to improve the color of egg yolk of chicken eggs, and is commercially available paprika pigment (trade name: Color Up; Ethoxyquin 0.5% Contained).

 Each powder was stored in an air atmosphere at 50 ° C and 75% humidity, and the change in carotenoid pigment content with time during storage for 1 month was investigated. The results are shown in Table 5.

[0041] According to Table 5, for commercial paprika dye containing 0.5% ethoxyquin, The prepared carotenoid pigment-containing composition powder (powdered rice bran powder) was found to have a stability improvement effect with a high carotenoid pigment residual ratio on both the 14th and 30th days.

[Table 5]

Industrial applicability

 The present invention can be effectively used in the field of food, including the feed field.

Claims

The scope of the claims  [1] Cultivating microorganisms that produce carotenoid pigments, and mixing rice bran with the resulting culture solution or culture concentrate of carotenoid pigments, followed by drying, providing excellent storage stability A method for producing a carotenoid pigment-containing composition.  [2] Microbial ability to produce carotenoid pigments Paracoccus, Hematococcus  The method according to claim 1, which belongs to the genus (Haematococcus) or the genus Ehaffia.  [3] Paracoccus genus bull. The paracoccus carotinifaciens is a method described by Tominaga 2¾. [4] The microbial power producing carotenoid pigments The base sequence of DNA corresponding to 16S ribosomal RNA is the base sequence set forth in SEQ ID NO: 1 or substantially homologous to the base sequence. The method of crab. [5] Microbial ability to produce carotenoid pigments E-396 strain (FERM BP-4283) and its mutant strains, A-581-1 strain (FERM BP-4671) and its mutant strains are selected The method of claim 4. [6] Cultivating microorganisms that produce carotenoid pigments, resulting in carotenoid pigments obtained by mixing rice bran with the culture solution or culture concentrate of the microorganisms, and then drying, resulting in excellent storage stability Carotenoid pigment-containing composition. [7] The composition according to claim 6, wherein the base sequence of DNA corresponding to the microbial power 16S ribosomal RNA producing carotenoid pigment is substantially the same as the base sequence set forth in SEQ ID NO: 1 or the base sequence . [8] Microbial ability to produce carotenoid pigments E-396 strain (FERM BP-4283) and its mutant strains, and A-581-1 strain (FERM BP-4671) and its mutant strains are selected The composition according to claim 7. [9] A feed comprising the composition according to any one of claims 6 to 8.