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WO2006036739A3 - Gene silencing - Google Patents

Gene silencing Download PDF

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Publication number
WO2006036739A3
WO2006036739A3 PCT/US2005/033992 US2005033992W WO2006036739A3 WO 2006036739 A3 WO2006036739 A3 WO 2006036739A3 US 2005033992 W US2005033992 W US 2005033992W WO 2006036739 A3 WO2006036739 A3 WO 2006036739A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene silencing
downregulates
constructs
producing
nucleic acid
Prior art date
Application number
PCT/US2005/033992
Other languages
French (fr)
Other versions
WO2006036739A2 (en
Inventor
Caius M T Rommens
Hua Yan
Oleg Bougri
Kathy M M Swords
Original Assignee
Simplot Co J R
Caius M T Rommens
Hua Yan
Oleg Bougri
Kathy M M Swords
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CN200580040336.XA priority Critical patent/CN101389755B/en
Priority to DK05803305.1T priority patent/DK1799028T3/en
Priority to AU2005289769A priority patent/AU2005289769B2/en
Priority to US11/662,872 priority patent/US8158414B2/en
Priority to PL05803305T priority patent/PL1799028T3/en
Priority to ES05803305.1T priority patent/ES2534426T3/en
Priority to EP05803305.1A priority patent/EP1799028B1/en
Application filed by Simplot Co J R, Caius M T Rommens, Hua Yan, Oleg Bougri, Kathy M M Swords filed Critical Simplot Co J R
Priority to CA2581440A priority patent/CA2581440C/en
Publication of WO2006036739A2 publication Critical patent/WO2006036739A2/en
Publication of WO2006036739A3 publication Critical patent/WO2006036739A3/en
Priority to AU2011200793A priority patent/AU2011200793B8/en
Priority to US13/432,356 priority patent/US20120258233A1/en
Priority to US14/617,488 priority patent/US9706724B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • A23L19/12—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops of potatoes
    • A23L19/18—Roasted or fried products, e.g. snacks or chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)

Abstract

The present invention relates to unique strategies and constructs for producing a nucleic acid product that downregulates or prevents expression of a desired target polynucleotide.
PCT/US2005/033992 2004-09-24 2005-09-23 Gene silencing WO2006036739A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CA2581440A CA2581440C (en) 2004-09-24 2005-09-23 Gene silencing
AU2005289769A AU2005289769B2 (en) 2004-09-24 2005-09-23 Gene silencing
US11/662,872 US8158414B2 (en) 2004-09-24 2005-09-23 Gene silencing
PL05803305T PL1799028T3 (en) 2004-09-24 2005-09-23 Gene silencing
ES05803305.1T ES2534426T3 (en) 2004-09-24 2005-09-23 Gene silencing
CN200580040336.XA CN101389755B (en) 2004-09-24 2005-09-23 Gene silencing
DK05803305.1T DK1799028T3 (en) 2004-09-24 2005-09-23 Gene silencing
EP05803305.1A EP1799028B1 (en) 2004-09-24 2005-09-23 Gene silencing
AU2011200793A AU2011200793B8 (en) 2004-09-24 2011-02-24 Gene silencing
US13/432,356 US20120258233A1 (en) 2004-09-24 2012-03-28 Gene silencing
US14/617,488 US9706724B2 (en) 2004-09-24 2015-02-09 Gene silencing

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US61263804P 2004-09-24 2004-09-24
US60/612,638 2004-09-24
US61995904P 2004-10-20 2004-10-20
US60/619,959 2004-10-20
US65360905P 2005-02-16 2005-02-16
US60/653,609 2005-02-16
US66807105P 2005-04-05 2005-04-05
US60/668,071 2005-04-05

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US11/662,872 A-371-Of-International US8158414B2 (en) 2004-09-24 2005-09-23 Gene silencing
US13/432,356 Division US20120258233A1 (en) 2004-09-24 2012-03-28 Gene silencing

Publications (2)

Publication Number Publication Date
WO2006036739A2 WO2006036739A2 (en) 2006-04-06
WO2006036739A3 true WO2006036739A3 (en) 2008-11-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/033992 WO2006036739A2 (en) 2004-09-24 2005-09-23 Gene silencing

Country Status (10)

Country Link
US (5) US7713735B2 (en)
EP (2) EP2444497B1 (en)
CN (1) CN101389755B (en)
AU (2) AU2005289769B2 (en)
CA (2) CA2581440C (en)
DK (1) DK1799028T3 (en)
ES (2) ES2530671T3 (en)
PL (2) PL1799028T3 (en)
PT (1) PT1799028E (en)
WO (1) WO2006036739A2 (en)

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AU2005289769B2 (en) 2010-12-02
US20060156428A1 (en) 2006-07-13
US20150296728A1 (en) 2015-10-22
CN101389755A (en) 2009-03-18
DK1799028T3 (en) 2015-03-23
ES2530671T3 (en) 2015-03-04
CA2581440A1 (en) 2006-04-06
EP2444497B1 (en) 2015-01-28
AU2011200793A8 (en) 2013-03-14
AU2011200793B8 (en) 2013-03-14
US9706724B2 (en) 2017-07-18
US20090100548A1 (en) 2009-04-16
CN101389755B (en) 2014-06-11
EP1799028A2 (en) 2007-06-27
PT1799028E (en) 2015-05-11
PL1799028T3 (en) 2015-06-30
WO2006036739A2 (en) 2006-04-06
EP2444497A1 (en) 2012-04-25
CA2940718C (en) 2019-06-18
AU2011200793B2 (en) 2012-11-22
US20120258233A1 (en) 2012-10-11
CA2940718A1 (en) 2006-04-06
US20090220670A1 (en) 2009-09-03
PL2444497T3 (en) 2015-04-30
AU2011200793A1 (en) 2011-03-24
ES2534426T3 (en) 2015-04-22
US8158414B2 (en) 2012-04-17
CA2581440C (en) 2016-11-29
US7713735B2 (en) 2010-05-11
EP1799028B1 (en) 2015-03-04
EP1799028A4 (en) 2009-11-11
AU2005289769A1 (en) 2006-04-06

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